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Autophosphorylation Activity of the Arabidopsis Ethylene Receptor
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor

... termed phosphorelays. In these pathways the receptors are often hybrid proteins containing a receiver domain at the carboxyl terminus of their kinase domain. After autophosphorylation of the histidine residue in the kinase domain, the phosphoryl group is transferred intra-molecularly to the receiver ...
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins

... the multigenic family of non-specific lipid transfer proteins (ns-LTPs). ns-LTPs are ubiquitous lipid binding proteins in plants. They have been comprehensively reviewed by Yamada (1992) or Kader (1996). Two main groups of ns-LTPs, ns-LTP1 and ns-LTP2 have been identified with molecular masses of ab ...
Molecular Models for Biochemistry at CMU
Molecular Models for Biochemistry at CMU

... standard biochemistry texts where more explanation is provided, but where interactive 3-D images of the molecules are not available. Please see viewing suggestions below before choosing a topic. SMALL MOLECULE GALLERY Introduction & Index to Chime displays of some textbook examples used in the Moder ...
INTRODUCTION - international journal of advances in
INTRODUCTION - international journal of advances in

... The redox energy from NADH and FADH2 is transferred to oxygen (O2) in several steps via the electron transport chain. This electron transport chain is shown in fig.3. These energy-rich molecules are produced within the matrix via the Citric Acid Cycle but are also produced in the cytoplasm by glycol ...
The regulation of receptor protein tyrosine
The regulation of receptor protein tyrosine

... The PTPRs can be dimerized or oligomerized via ligand binding on extracellular domains (Fukada et al., 2006; Perez-Pinera et al., 2007). This interaction can change the structure of the intracellular domain and inactivate phosphatase activity. Several studies have proven that the dimerization of the ...
Are GMOs Safe?
Are GMOs Safe?

... 10) One study suggested that Bt toxin has toxic effects on human cells in vitro, causing them to die prematurely. (11) This could cause damage to gut endothelial cells if the toxicity is found to occur in vivo. The potential intestinal effects of GMO consumption go beyond Bt toxin. Some argue that g ...
Medical Biochemistry: Third Edition Chapter 2 P. 9: Ka = [H+][A
Medical Biochemistry: Third Edition Chapter 2 P. 9: Ka = [H+][A

... Fig.10.7: Structure B is not explained. Moreover, the second structure (B) of cholic acid is missing side groups: ...
Topology and Phosphorylation of Soybean Nodulin
Topology and Phosphorylation of Soybean Nodulin

... is derived from plasma membrane of the host cell and contains several protein bands common to the plasma membrane. A 26-kD peptide is also present in the plasma membrane of uninfected soybean root (Fortin et al., 1985). To determine its identity, antibodies were raised against a synthetic peptide co ...
Cyanuric acid hydrolase: evolutionary innovation by structural
Cyanuric acid hydrolase: evolutionary innovation by structural

... scattering data, these observations suggest that the native metal for AtzD is magnesium. The three potential catalytic dyads were investigated by site-directed mutagenesis. Amino acid substitutions at Lys42 (Ala), Ser85 (Ala), Lys162 (Ala, Arg), Ser233 (Ala), Lys296 (Ala, Arg) and Ser344 (Ala) yield ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... helix, the helices built from the Step 2 were optimized separately. In this procedure, we first use SCWRL for side-chain placement, then carry out molecular dynamics (MD) (either Cartesian or torsional MD called NEIMO) simulations at 300 K for 500 ps, then choose the structure with the lowest total ...
Structural Prediction of Membrane
Structural Prediction of Membrane

... [l, 21. With their advent have come many secondary-structure prediction methods which require only a knowledge of the amino acid sequence (cf. 13 - 51). These techniques generally rely on a statistical or informational analysis of the frequency with which the 20 amino acids appear within the observe ...
33. Botulinum Toxins
33. Botulinum Toxins

... The surgical care he received was surprisingly good, even when judged by today’s standards. Heydrich’s initial postoperative course was satisfactory, although he was modestly febrile and there was drainage from the wound of entrance. His condition worsened suddenly on the seventh postoperative day a ...
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION

... studies have shown that a substitution of the serine by alanine in the human NaS1 resulted in an increased Km for sulfate transport 13. ...
Molecular analysis of an operon in Bacillus subtilis
Molecular analysis of an operon in Bacillus subtilis

... present. In addition, between the Walker motifs I and 11, EcsA contains a glutamine- and glycine-rich motif (consensus L/FSGGQQ/R/KQR) that is well-conserved in the ATP-binding components of ABC-transporters (Ames e t al., 1992). Homology that extends beyond the ATP-binding motifs was observed betwe ...
Functional characterisation and cell walll interactions of
Functional characterisation and cell walll interactions of

... the cell wall, thus affecting the interaction with inert surfaces (157). They could also be involved in adhesion to other microorganisms or to eukaryotic cells (e.g. colonization of the ...
Short transmembrane domains with high
Short transmembrane domains with high

... with the COPI-interacting protein Vps74 (Tu et al., 2008). Other reports assign importance to the TMD for localization of glycosyltransferases (GTs) in the Golgi (Opat et al., 2001; Tang et al., 1992; Teasdale et al., 1992). This capacity was attributed to particular amino acid residues in the TMD ( ...
Cell membrane
Cell membrane

... Examples: Ions ,Glucose or amino acids moving from blood into a cell. There are two kinds of transport protein: 1- Channel Proteins : form a water-filled pore or channel in the membrane. This allows charged substances (usually ions) to diffuse across membranes. Most channels can be gated (opened or ...
Test 1 Study Guide  Chapter  1 – Introduction
Test 1 Study Guide Chapter 1 – Introduction

... a. Nucleus in center – made from protons (+) and neutrons (=). Both have an atomic weight of 1 b. Shell on outside. Made up of electrons (-) with close to zero weight. Electrons orbit the nucleus. (Fig. 2.1) c. Common elements (Tab. 2.1) d. Isotopes are atoms with a different number of neutrons. E.g ...
Test 1 Study Guide
Test 1 Study Guide

... a. Nucleus in center – made from protons (+) and neutrons (=). Both have an atomic weight of 1 b. Shell on outside. Made up of electrons (-) with close to zero weight. Electrons orbit the nucleus. (Fig. 2.1) c. Common elements (Tab. 2.1) d. Isotopes are atoms with a different number of neutrons. E.g ...
Interacting specificity of a histidine kinase and its cognate response
Interacting specificity of a histidine kinase and its cognate response

... formed to generate PrrB–DevS chimeric proteins. Two rounds of PCR were carried out using pfu polymerase. The plasmids pUI1643 and pBSC were used as the templates for amplification of portions of prrB and devS, respectively. Two primary PCR reactions were performed with the primers PrrBc(BamHI)+ and ...
Molecular architecture of the pyruvate dehydrogenase complex
Molecular architecture of the pyruvate dehydrogenase complex

... to the 2-fold axis of symmetry [21]. Thus the association of a second molecule of E2-SBD with either E1 or E3 is not possible. Occupation of both binding sites would result in steric clashes in one of the E2-SBD loop regions. In contrast, it has been known for a long time that a total of 30 E1 and s ...
Classification and substrate head-group specificity of membrane
Classification and substrate head-group specificity of membrane

... the ω3-LCPUFAs biosynthesis pathway [20,63,64]. This is because some Δ6 desaturases utilize acyl-PC substrates, whereas the elongases at the next step utilize acyl-CoA substrates [20]. Thus, for acyl-PC-specific Δ6 desaturases, the product of the Δ6 desaturation has to be converted into an acyl-CoA ...
See Source - Pentelute Lab
See Source - Pentelute Lab

... milligram scale using the approach shown in Figure 2a. In particular, a one-pot method was employed whereby we first removed the N-terminal SUMO tag with SUMO protease and subsequently added SrtA*, Ni-NTA agarose beads, and oligoglycine αthioester peptide. After completion of the SrtA*-mediated liga ...
Identification of Surface Residues Involved in Protein
Identification of Surface Residues Involved in Protein

... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
human-physiology-ii-lecture-endomembrane
human-physiology-ii-lecture-endomembrane

... Source: Collected from different sources on the internet-http://koning.ecsu.ctstateu.edu/cell/cell.html ...
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Anthrax toxin



Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis—the causative agent of anthrax. The toxin was first discovered by Harry Smith in 1954. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.
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