Stage and developmental specific gene expression during
... mice. Therefore, it can be concluded that the DNA sequences located between the 400 bp and 900 bp upstream of ATG can function as enhancer elements. For further characterization of DNA cis-acting elements we sequenced about 1 kb of the 5' flanking region of the proaccrosin gene of different mammals. ...
... mice. Therefore, it can be concluded that the DNA sequences located between the 400 bp and 900 bp upstream of ATG can function as enhancer elements. For further characterization of DNA cis-acting elements we sequenced about 1 kb of the 5' flanking region of the proaccrosin gene of different mammals. ...
MembraneStructure
... • To work properly with active enzymes and appropriate permeability, membrane must be fluid, about as fluid as salad oil. • Cells can alter the lipid composition of membranes to compensate for changes in fluidity caused by changing temperatures. • For example, cold-adapted organisms, such as winter ...
... • To work properly with active enzymes and appropriate permeability, membrane must be fluid, about as fluid as salad oil. • Cells can alter the lipid composition of membranes to compensate for changes in fluidity caused by changing temperatures. • For example, cold-adapted organisms, such as winter ...
The dark side of ribosome biogenesis
... Combining crystals, yeast, and red blood cells The impact of DBA mutations on the function of RPS19 was studied by determining the atomic structure of RPS19 using X-Ray crystallography. This structure revealed two classes of mutations, affecting either the stability of RPS19 or its capacity to inter ...
... Combining crystals, yeast, and red blood cells The impact of DBA mutations on the function of RPS19 was studied by determining the atomic structure of RPS19 using X-Ray crystallography. This structure revealed two classes of mutations, affecting either the stability of RPS19 or its capacity to inter ...
Gene Regulation
... inactivated lacIrare dominant lacc mutations... ...all mapped to lacI inactived LacI protein but it could still form tetramers As a Tool in Molecular Biology lac promoter is inducible. Allowing production of toxic genes IPTG, nonclevable derivative of allolactose Several colorimetric substrates exis ...
... inactivated lacIrare dominant lacc mutations... ...all mapped to lacI inactived LacI protein but it could still form tetramers As a Tool in Molecular Biology lac promoter is inducible. Allowing production of toxic genes IPTG, nonclevable derivative of allolactose Several colorimetric substrates exis ...
S1. Comparison of complex functions in MCL-GO
... are conserved in their biological processes in E. coli than yeast. Conversely fewer superfamilies are conserved in molecular function and cellular component in E. coli than yeast. Notice that the numbers are correlated with organismal complexity. The results suggest that more complex organisms have ...
... are conserved in their biological processes in E. coli than yeast. Conversely fewer superfamilies are conserved in molecular function and cellular component in E. coli than yeast. Notice that the numbers are correlated with organismal complexity. The results suggest that more complex organisms have ...
Seq_pattern_II
... not yet in plants. This gene is involved in an interesting biological process (e.g., apoptosis). You are interested in finding the orthologous gene in Arabidopsis. However, BLAST search using each of the known sequences failed to identify an Arabidopsis homologue. What else can you try? ...
... not yet in plants. This gene is involved in an interesting biological process (e.g., apoptosis). You are interested in finding the orthologous gene in Arabidopsis. However, BLAST search using each of the known sequences failed to identify an Arabidopsis homologue. What else can you try? ...
Will Entrez Find Every Sequence Record?
... • The sequences that you miss are the ones that have not been annotated with the current official gene symbol in the “gene” field • DO NOT use this method if you need to find every sequence for a particular gene ...
... • The sequences that you miss are the ones that have not been annotated with the current official gene symbol in the “gene” field • DO NOT use this method if you need to find every sequence for a particular gene ...
Gene Regulation
... arac muants are rare because the mutation must make AraC active without binding arabinose Inactivation of araC (unlike lacI) produces an ara- phenotype AraC must also be an antiactivator since... araCc mutations should be dominant (but they are not). IV. The trp operon (Negative regulation and trans ...
... arac muants are rare because the mutation must make AraC active without binding arabinose Inactivation of araC (unlike lacI) produces an ara- phenotype AraC must also be an antiactivator since... araCc mutations should be dominant (but they are not). IV. The trp operon (Negative regulation and trans ...
Poster Category 2: Sex and Sexual Development
... In the smut fungus Ustilago maydis, pathogenic development is controlled by the b‐mating type locus, orchestrating a regulatory network consisting of different transcription factors. A key factor for the regulation of this circuit is the Clp1 protein. Via physical interactions with t ...
... In the smut fungus Ustilago maydis, pathogenic development is controlled by the b‐mating type locus, orchestrating a regulatory network consisting of different transcription factors. A key factor for the regulation of this circuit is the Clp1 protein. Via physical interactions with t ...
Gene Section SDHD (succinate dehydrogenase complex II, subunit D, integral membrane protein)
... autosomal dominant disorder. The disease is transmitted through fathers and no disease phenotype is transmetted maternally in accordance with a maternal genomic imprinting. Paragangliomas are slow growing highly vascular tumor, usually benign, derived from crest-neural cells. They are preferentially ...
... autosomal dominant disorder. The disease is transmitted through fathers and no disease phenotype is transmetted maternally in accordance with a maternal genomic imprinting. Paragangliomas are slow growing highly vascular tumor, usually benign, derived from crest-neural cells. They are preferentially ...
Leukaemia Section t(5;14)(q33;q32) PDGFRB/TRIP11 Atlas of Genetics and Cytogenetics in Oncology and Haematology
... N-term leucine zipper from CEV14 fused to the transmembrane domain and the Tyr kinase domain of PDGFRb in C-term; the reciprocal transcript is not expressed; breakpoints at amino acids 1936 of PDGFRb and 567 of CEV14. Oncogenesis Ectopic constitutive tyrosine kinase activation of PDGFRb may occur. ...
... N-term leucine zipper from CEV14 fused to the transmembrane domain and the Tyr kinase domain of PDGFRb in C-term; the reciprocal transcript is not expressed; breakpoints at amino acids 1936 of PDGFRb and 567 of CEV14. Oncogenesis Ectopic constitutive tyrosine kinase activation of PDGFRb may occur. ...
Mechanisms of mitochondrial protein import
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
Background. We previously mapped an autosomal recessive form of
... three inbred kindreds from Tunisia. The defect and pathogenic mechanism underlying this ALS remain to be elucidated. Hypothesis. Loss of function mutations in a gene in this 6cM minimum candidate region cause ALS5. Methods. Whole-exome sequencing and Sanger sequencing were used to identify mutations ...
... three inbred kindreds from Tunisia. The defect and pathogenic mechanism underlying this ALS remain to be elucidated. Hypothesis. Loss of function mutations in a gene in this 6cM minimum candidate region cause ALS5. Methods. Whole-exome sequencing and Sanger sequencing were used to identify mutations ...
Abstract는
... Downregulation of DBC1 expression enhances the deacetylation of SIRT1-target proteins such as p53 and FOXO, and thus potentiates SIRT1-dependent inhibition of apoptosis induced by cellular stress, suggesting a possible role of DBC1 as a tumor suppressor. In addition, we have shown the novel function ...
... Downregulation of DBC1 expression enhances the deacetylation of SIRT1-target proteins such as p53 and FOXO, and thus potentiates SIRT1-dependent inhibition of apoptosis induced by cellular stress, suggesting a possible role of DBC1 as a tumor suppressor. In addition, we have shown the novel function ...
Fig. 1. - iGEM 2010
... proteins at room temperature only, thus production does not occur at incubation temperature (37°C). In order to make an environmentally safe insecticide, our design also incorporates a genetic circuit controlling the population size of E. coli, thus a surplus will never exist as E. coli population i ...
... proteins at room temperature only, thus production does not occur at incubation temperature (37°C). In order to make an environmentally safe insecticide, our design also incorporates a genetic circuit controlling the population size of E. coli, thus a surplus will never exist as E. coli population i ...
Leukaemia Section t(3;18)(q26;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0318q26q11ID1283.html DOI: 10.4267/2042/38563 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0318q26q11ID1283.html DOI: 10.4267/2042/38563 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Transport of proteins into and across the thylakoid membrane
... invariably synthesized without signal-type presequences. Apparently, these have been acquired after the transfer of the genes to the nucleus and the insertion mechanism thus differs markedly in this respect. Since the mature proteins appear very similar in structural terms, it is unclear why the imp ...
... invariably synthesized without signal-type presequences. Apparently, these have been acquired after the transfer of the genes to the nucleus and the insertion mechanism thus differs markedly in this respect. Since the mature proteins appear very similar in structural terms, it is unclear why the imp ...
End Semester Examination (July- Dec, 2015) BT 305: Enzyme
... (a) Explain catabolism and anabolism with an example. 2 (b) At the time of enzyme isolation, cell lysis methods are required. Explain briefly about cell lysis methods. ...
... (a) Explain catabolism and anabolism with an example. 2 (b) At the time of enzyme isolation, cell lysis methods are required. Explain briefly about cell lysis methods. ...
1 Problem set 3 Due dates: Official date is 12 Dec. However I will
... preparation for Southern analysis. Northern analysis on the mRNA was also performed, as was a Western analysis employing antibody to enzyme m. Based on the results shown to the right, say why person 'B' has no symptoms. Say why some of person 'B's children do have symptoms. For each of people 'C', ' ...
... preparation for Southern analysis. Northern analysis on the mRNA was also performed, as was a Western analysis employing antibody to enzyme m. Based on the results shown to the right, say why person 'B' has no symptoms. Say why some of person 'B's children do have symptoms. For each of people 'C', ' ...
The Operon 操縱元
... The repressor protein is a tetramer of identical subunits coded by the gene lacI. ...
... The repressor protein is a tetramer of identical subunits coded by the gene lacI. ...
BIRKBECK COLLEGE
... You will be given a start time with your exam instructions Students will be required to answer 10 out of 15 questions. All questions carry 10 marks each. Each question must start on a new page and the question number written at the top of each sheet. ...
... You will be given a start time with your exam instructions Students will be required to answer 10 out of 15 questions. All questions carry 10 marks each. Each question must start on a new page and the question number written at the top of each sheet. ...
Dankesrede von Prof. Dr. Charles Dinarello anlässlich der
... increasingly clear that the molecule did more than cause fever: endogenous pyrogen killed the insulin-producing cells, broke down cartilage in joints, induced sleep, depressed appetite but stimulated the liver and increased antibody production. It caused the loss of muscle mass, blood pressure to fa ...
... increasingly clear that the molecule did more than cause fever: endogenous pyrogen killed the insulin-producing cells, broke down cartilage in joints, induced sleep, depressed appetite but stimulated the liver and increased antibody production. It caused the loss of muscle mass, blood pressure to fa ...
UBIQUITIN AT FOX CHASE
... action of E1, glutathione, and the hydrolase results in a futile cycle converting ATP to AMP+PPi, Scheme 2. AMP-Ub is normally too tightly bound to E1 to lead to a futile cycle of its own in the presence of an active nucleophile. Amides of Ub were not available to test as substrates of the new Ubiqu ...
... action of E1, glutathione, and the hydrolase results in a futile cycle converting ATP to AMP+PPi, Scheme 2. AMP-Ub is normally too tightly bound to E1 to lead to a futile cycle of its own in the presence of an active nucleophile. Amides of Ub were not available to test as substrates of the new Ubiqu ...
Full Text - International Journal of Livestock Research
... fibrinogen, collagen, vitronectin, laminin, elastin, von Willebrand factor etc. Staphylococcal surface adhesion molecules like fibronectin, fibrinogen and collagen binding proteins have been shown to contribute to persistence of bacteria by adhering to sub epithelial tissue components after epitheli ...
... fibrinogen, collagen, vitronectin, laminin, elastin, von Willebrand factor etc. Staphylococcal surface adhesion molecules like fibronectin, fibrinogen and collagen binding proteins have been shown to contribute to persistence of bacteria by adhering to sub epithelial tissue components after epitheli ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.