National Microbial Pathogen Database Resource (NMPDR): a
... complete. Genome data include the contig sequences, defined sequence features such as genes that encode RNAs or proteins, and annotations associated with each feature. Annotations include accurately determined boundaries of the features, and the assignment of a functional name to encoded proteins. N ...
... complete. Genome data include the contig sequences, defined sequence features such as genes that encode RNAs or proteins, and annotations associated with each feature. Annotations include accurately determined boundaries of the features, and the assignment of a functional name to encoded proteins. N ...
Full-Text PDF
... DCMECs from three biological replicates were provided by our laboratory. These cells were cultured according to the previous report [29]. DCMECs were grown in Dulbecco Modified Eagle Medium: Nutrient Mixture 12 (DMEM: F12) containing 10% fetal bovine serum (FBS), insulin (bovine, 5 μg mL−1), hydroco ...
... DCMECs from three biological replicates were provided by our laboratory. These cells were cultured according to the previous report [29]. DCMECs were grown in Dulbecco Modified Eagle Medium: Nutrient Mixture 12 (DMEM: F12) containing 10% fetal bovine serum (FBS), insulin (bovine, 5 μg mL−1), hydroco ...
Biological Chemistry: Engineering New Functions for Natural Systems
... simulating biochemical effects of some diseases . A zinc finger nuclease is even in clinical trials for HIV gene therapy, as of May 2012 .[3] Scientists engineer the binding domain on these zinc finger proteins so they can control where to insert a new gene in a chromosome . But the nucleases tend ...
... simulating biochemical effects of some diseases . A zinc finger nuclease is even in clinical trials for HIV gene therapy, as of May 2012 .[3] Scientists engineer the binding domain on these zinc finger proteins so they can control where to insert a new gene in a chromosome . But the nucleases tend ...
Large Biological Molecules Organic Chemistry, Hydrocarbons
... 44. Briefly explain why proteins, carbohydrates and nucleic acids are considered biomolecular polymers. 45. Which of the three components of an amino acid is most comparable in functionality to the nitrogen base of a nucleotide? 46. Amino acids each have an H atom on one and an OH group on the other ...
... 44. Briefly explain why proteins, carbohydrates and nucleic acids are considered biomolecular polymers. 45. Which of the three components of an amino acid is most comparable in functionality to the nitrogen base of a nucleotide? 46. Amino acids each have an H atom on one and an OH group on the other ...
www.njctl.org Biology Large Biological Molecules
... 44. Briefly explain why proteins, carbohydrates and nucleic acids are considered biomolecular polymers. 45. Which of the three components of an amino acid is most comparable in functionality to the nitrogen base of a nucleotide? 46. Amino acids each have an H atom on one and an OH group on the other ...
... 44. Briefly explain why proteins, carbohydrates and nucleic acids are considered biomolecular polymers. 45. Which of the three components of an amino acid is most comparable in functionality to the nitrogen base of a nucleotide? 46. Amino acids each have an H atom on one and an OH group on the other ...
electrical engineering
... mechanisms that counter most mutations. However, some mutations can cause _________________. Many mutations occur when cells divide as a result of errors during DNA replication. Others are caused by environmental agents, called __________________. Since mutations can cause cancer, these agents are a ...
... mechanisms that counter most mutations. However, some mutations can cause _________________. Many mutations occur when cells divide as a result of errors during DNA replication. Others are caused by environmental agents, called __________________. Since mutations can cause cancer, these agents are a ...
How Do Plant Mitochondria Avoid Importing Chloroplast Proteins
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
Bionemo: molecular information on
... elements. From there, the user can navigate to different views. Then, by clicking on a reaction name, the user will be taken to a new page showing all the enzymatic complexes able to perform that reaction, and from there to one describing proteins, their coding genes, their transcriptional organizati ...
... elements. From there, the user can navigate to different views. Then, by clicking on a reaction name, the user will be taken to a new page showing all the enzymatic complexes able to perform that reaction, and from there to one describing proteins, their coding genes, their transcriptional organizati ...
Supplementary Table S2 (doc 37K)
... W312 residue lays at the entrance of the active site cavity. In particular, the analysis predicted that this aromatic residue may form a stacking interaction with the guanidine group of the residue R285. Comparing the structures of the apo-enzyme to the one of the protein bound to N-butyl-deoxyn ...
... W312 residue lays at the entrance of the active site cavity. In particular, the analysis predicted that this aromatic residue may form a stacking interaction with the guanidine group of the residue R285. Comparing the structures of the apo-enzyme to the one of the protein bound to N-butyl-deoxyn ...
Title Screening candidate genes required for CENP
... Centromere is the specialized chromosomal region where the assembly of a large protein complex called the kinetochore takes place. The kinetochore functions in mediating the attachment of spindle fibres to sister chromatids during cell division. Successful formation of a complete kinetochore ensures ...
... Centromere is the specialized chromosomal region where the assembly of a large protein complex called the kinetochore takes place. The kinetochore functions in mediating the attachment of spindle fibres to sister chromatids during cell division. Successful formation of a complete kinetochore ensures ...
Data Mining in Ensembl with BioMart
... http://www.biomart.org/biomart/martview http://www.ensembl.org/biomart/martview ...
... http://www.biomart.org/biomart/martview http://www.ensembl.org/biomart/martview ...
Slide 1
... Protein structure classification •Classification systems allows identification of relationships between structures •Provide evolutionary view of all structures •Newly solved structures can be fitted into hierarchy, defining possible functions SCOP (Structural Classification of Proteins) Manual; exa ...
... Protein structure classification •Classification systems allows identification of relationships between structures •Provide evolutionary view of all structures •Newly solved structures can be fitted into hierarchy, defining possible functions SCOP (Structural Classification of Proteins) Manual; exa ...
T. brucei basal body component - Journal of Cell Science
... with anti-TBBC and either anti-BBA4 or anti-γ-tubulin ...
... with anti-TBBC and either anti-BBA4 or anti-γ-tubulin ...
Protein-Protein Interactions: Stability, Function and Landscape
... The Accessible surface area (ASA) of the complexes is calculated using an implementaion of the Lee and Richards (1971) algorithm devloped by Hubbard (1992). With a probe sphere, of radius 1.4 angstroms, the ASA was defined as the surface mapped out by the centre of the probe as if it were rolled aro ...
... The Accessible surface area (ASA) of the complexes is calculated using an implementaion of the Lee and Richards (1971) algorithm devloped by Hubbard (1992). With a probe sphere, of radius 1.4 angstroms, the ASA was defined as the surface mapped out by the centre of the probe as if it were rolled aro ...
In GAS, we have identified four Rgg regulators (Rgg1
... One of the Rggs (Rgg4, renamed ComR) is conserved throughout pyogenic, mutans and bovis species of streptococci. We have determined that a small open reading frame, found adjacent to comR in each streptococcal genome, encodes a pro-peptide (ComS) that is secreted from cells. Processing of the propet ...
... One of the Rggs (Rgg4, renamed ComR) is conserved throughout pyogenic, mutans and bovis species of streptococci. We have determined that a small open reading frame, found adjacent to comR in each streptococcal genome, encodes a pro-peptide (ComS) that is secreted from cells. Processing of the propet ...
The antibody validation experiments were essentially performed as
... 14. A substrate for chemiluminescence (Immobilon Western Chemiluminescence HRP Substrate, Millipore) was used for detection and the image was acquired using a ChemiDoc MP and Image Lab 5.2. 15. The relative signal intensity of the band in the siRNA lane was compared to the band in the negative contr ...
... 14. A substrate for chemiluminescence (Immobilon Western Chemiluminescence HRP Substrate, Millipore) was used for detection and the image was acquired using a ChemiDoc MP and Image Lab 5.2. 15. The relative signal intensity of the band in the siRNA lane was compared to the band in the negative contr ...
Complete genome sequence of an M1 strain of Streptococcus
... (rpoD)] as well as an identifiable minor factor (homolog of E). The E (also known as 24) is one of the major factors necessary for transcription of heat-induced proteins in E. coli (20), and the homolog found in S. pyogenes may play a similar role when the organism encounters elevated temperatu ...
... (rpoD)] as well as an identifiable minor factor (homolog of E). The E (also known as 24) is one of the major factors necessary for transcription of heat-induced proteins in E. coli (20), and the homolog found in S. pyogenes may play a similar role when the organism encounters elevated temperatu ...
Text S1 Y2H Interactome Mapping The literature was curated to
... (fraction) of Hrs-bound EsxGMt EsxHMt to which the Hill equation was fitted. However, under current experimental conditions, we may not be able to accurately measure the bound fraction with 100% efficiency, and the system may or may not be at steady-state equilibrium. For this reason, we also took i ...
... (fraction) of Hrs-bound EsxGMt EsxHMt to which the Hill equation was fitted. However, under current experimental conditions, we may not be able to accurately measure the bound fraction with 100% efficiency, and the system may or may not be at steady-state equilibrium. For this reason, we also took i ...
Journal of Bacteriology
... Isolation of a cosmid clone involved in the production of outer membrane antigen group III. For isolation of a clone involved in production of antigen group III of R. leguminosarum bv. viciae strain 248, we made use of the strainspecific reactivity of one of the monoclonal antibodies that recognize ...
... Isolation of a cosmid clone involved in the production of outer membrane antigen group III. For isolation of a clone involved in production of antigen group III of R. leguminosarum bv. viciae strain 248, we made use of the strainspecific reactivity of one of the monoclonal antibodies that recognize ...
Modular Structure of Transcription Factors: Implications for Gene
... Modular protein domains or structural units are ideally suited for complexes in gene regulation, because they facilitate the design of interacting protein structures that are so important for cooperative and/or allosteric interactions. For example, interactions mediated by the C-terminal domain of h ...
... Modular protein domains or structural units are ideally suited for complexes in gene regulation, because they facilitate the design of interacting protein structures that are so important for cooperative and/or allosteric interactions. For example, interactions mediated by the C-terminal domain of h ...
Leukaemia Section t(3;11)(q26;p15) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0311q26p15ID1474.html DOI: 10.4267/2042/38480 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
... Online updated version: http://AtlasGeneticsOncology.org/Anomalies/t0311q26p15ID1474.html DOI: 10.4267/2042/38480 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Expression of Cloned Gene
... solubility by expressing the protein at a lower temperature. Also expression of your target protein as a fusion protein with a highly soluble partner such as glutathione-Stransferase (GST), maltose binding protein (MBP), or DsbA can improve its solubility. Often, however, it is better to change to a ...
... solubility by expressing the protein at a lower temperature. Also expression of your target protein as a fusion protein with a highly soluble partner such as glutathione-Stransferase (GST), maltose binding protein (MBP), or DsbA can improve its solubility. Often, however, it is better to change to a ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.