Supplement Material…
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
Establishment of an experimental system allowing immobilization of
... Gram-positive bacteria code for one or more enzymes termed sortases which catalyze the covalent anchoring of substrate proteins on their cell wall. They recognize an amino acid sequence designated sorting motif, present close to the C-terminal end of the substrate proteins, cleave within this motif ...
... Gram-positive bacteria code for one or more enzymes termed sortases which catalyze the covalent anchoring of substrate proteins on their cell wall. They recognize an amino acid sequence designated sorting motif, present close to the C-terminal end of the substrate proteins, cleave within this motif ...
No Slide Title
... Inclusion cell disease (I cell disease) - rare disorder in which almost all hydrolytic enzymes are missing from lysosome I cell disease - single gene, recessive defect ...
... Inclusion cell disease (I cell disease) - rare disorder in which almost all hydrolytic enzymes are missing from lysosome I cell disease - single gene, recessive defect ...
Prokaryotic Gene Regulation (PowerPoint) Gulf Coast 2012
... Assign students into groups to discuss the sequence of the events that must happen in the model ...
... Assign students into groups to discuss the sequence of the events that must happen in the model ...
The Drosophila Ribosomal Protein S6 Gene Includes a 3
... can result in proteins with repeated functional domains. In work presented elsewhere (Stewart and Denell 1993), we have shown that two transposon-induced mutations causing a loss of growth control of the Drosophila larval hematopoietic organs affect the gene encoding ribosomal protein S6 (rpS6 ) . T ...
... can result in proteins with repeated functional domains. In work presented elsewhere (Stewart and Denell 1993), we have shown that two transposon-induced mutations causing a loss of growth control of the Drosophila larval hematopoietic organs affect the gene encoding ribosomal protein S6 (rpS6 ) . T ...
TissueNet database of human tissue protein–protein interactions
... (GO) tab provides the GO annotations of proteins selected in the network view (20). The ‘Chemicals’ tab lists chemicals known to interact with selected proteins, which might suggest candidates for experimental manipulation (21). Lastly, the ‘Tissues’ tab lists per tissue the subset of proteins that ...
... (GO) tab provides the GO annotations of proteins selected in the network view (20). The ‘Chemicals’ tab lists chemicals known to interact with selected proteins, which might suggest candidates for experimental manipulation (21). Lastly, the ‘Tissues’ tab lists per tissue the subset of proteins that ...
DNA Chip Analysis and Bioinformatics
... Choose at least one sequence to analyze. Copy the sequence. Go to the National Center for Biotechnology Information (NCBI) site http://www.ncbi.nlm.nih.gov/ From the menu on the right click on “BLAST” (Basic Local Alignment Search Tool). Choose the BASIC BLAST program "Nucleotide Blast". Paste the p ...
... Choose at least one sequence to analyze. Copy the sequence. Go to the National Center for Biotechnology Information (NCBI) site http://www.ncbi.nlm.nih.gov/ From the menu on the right click on “BLAST” (Basic Local Alignment Search Tool). Choose the BASIC BLAST program "Nucleotide Blast". Paste the p ...
Enzyme Structure and Function11
... “Lock and Key” no longer match; enzyme can’t bind to substrate when they collide. ...
... “Lock and Key” no longer match; enzyme can’t bind to substrate when they collide. ...
Chemistry In Your Life
... • Long strands of DNA are stored within the nucleus of most cells in the form of chromosomes. • The number of chromosomes varies with species (Human = 26 or 23 pairs) • The DNA within a chromosome is divided into many segments, many of which carry specific information in the form of genes. • Genes a ...
... • Long strands of DNA are stored within the nucleus of most cells in the form of chromosomes. • The number of chromosomes varies with species (Human = 26 or 23 pairs) • The DNA within a chromosome is divided into many segments, many of which carry specific information in the form of genes. • Genes a ...
Regulation-of-Gene
... This provides an exception to the general rule that genes on an operon are transcribed into a single mRNA ...
... This provides an exception to the general rule that genes on an operon are transcribed into a single mRNA ...
מצגת של PowerPoint - Tel Aviv University
... the organism to produce double the amount of the derived protein. 3. The two copies can diverge and each one will specialize in a different function. ...
... the organism to produce double the amount of the derived protein. 3. The two copies can diverge and each one will specialize in a different function. ...
Protein Phosphorylation and a Novel Phosphatase
... Protein phosphatases catalyze the dephosphorylation half of the cycle. These proteins function to remove phosphate groups from serine, threonine, or tyrosine residues. Two major phosphatase families are PP1, protein phosphatase type 1, and PP2, or protein phosphatase type 2. These two subsets are fo ...
... Protein phosphatases catalyze the dephosphorylation half of the cycle. These proteins function to remove phosphate groups from serine, threonine, or tyrosine residues. Two major phosphatase families are PP1, protein phosphatase type 1, and PP2, or protein phosphatase type 2. These two subsets are fo ...
(FADD) in a total cell lysate
... PS For a given tryptic peptide, the intensity of signal depends on the chosen transition. Transition A gives higher signals than transition D. ...
... PS For a given tryptic peptide, the intensity of signal depends on the chosen transition. Transition A gives higher signals than transition D. ...
No Slide Title
... 1 . AD related genes are clustered in a subnetwork of protein interactions, 2 . Randomly selected seed genes are uniformly distributed around the network. -Use set of “seed” genes derived from expert list, or linkage results, or 4 known genes - score each protein in network by how close it is to the ...
... 1 . AD related genes are clustered in a subnetwork of protein interactions, 2 . Randomly selected seed genes are uniformly distributed around the network. -Use set of “seed” genes derived from expert list, or linkage results, or 4 known genes - score each protein in network by how close it is to the ...
Show DNA to Protein HC
... rejoins remaining exons together (recognize special sequences) • Ribozymes – mRNA that catalyzes its own intron removal (not all enzymes are proteins) ...
... rejoins remaining exons together (recognize special sequences) • Ribozymes – mRNA that catalyzes its own intron removal (not all enzymes are proteins) ...
GENE WIKI CONCEPTUAL OVERVIEW
... Rank & Reason The scientist will also be able to rank and recommend additional research for a given gene or protein. S/he will be able to give very specific information on his/her reasons and requested research. Supporting data or information can be attached. ...
... Rank & Reason The scientist will also be able to rank and recommend additional research for a given gene or protein. S/he will be able to give very specific information on his/her reasons and requested research. Supporting data or information can be attached. ...
Conjugating Enzymes Belonging to the UbcP4/E2
... genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differe ...
... genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differe ...
Introduction to Proteins II
... a. -strand depicted as flat arrow. b. -helix depicted as barrel. Be familiar with these. c. A -sheet, the way the atoms align, is shown here. (More in August 12 lecture) The Sequence of Amino Acids in a Protein [S23] a. The exact sequence of amino acids is unique for each protein. Protein Structu ...
... a. -strand depicted as flat arrow. b. -helix depicted as barrel. Be familiar with these. c. A -sheet, the way the atoms align, is shown here. (More in August 12 lecture) The Sequence of Amino Acids in a Protein [S23] a. The exact sequence of amino acids is unique for each protein. Protein Structu ...
Lipid-modified morphogens: functions of fats - treisman lab
... involve the serine-linked palmitoleic acid. The retromer complex is also required for Wnt secretion, owing to its function in recycling Wls from the plasma membrane through endosomes to the Golgi [50]. Intriguingly, the recent finding that Drosophila WntD, a non-lipidated Wnt involved in dorsal-vent ...
... involve the serine-linked palmitoleic acid. The retromer complex is also required for Wnt secretion, owing to its function in recycling Wls from the plasma membrane through endosomes to the Golgi [50]. Intriguingly, the recent finding that Drosophila WntD, a non-lipidated Wnt involved in dorsal-vent ...
Abstract The family Solanaceae is unique in representing crops like
... forefront of scientific importance in the fields of comparative genomics and plastome biology research with other model organisms that are well studied, such as rice, Arabidopsis and tobacco, respectively. An understanding of the plastome biology of the Solanaceae makes this an ideal time to link pl ...
... forefront of scientific importance in the fields of comparative genomics and plastome biology research with other model organisms that are well studied, such as rice, Arabidopsis and tobacco, respectively. An understanding of the plastome biology of the Solanaceae makes this an ideal time to link pl ...
template - ISRFG 2016
... In rice roots, ground tissue (GT) is composed of one layer of exodermis, one layer of sclerenchyma, several layers of cortex and one layer of endodermis. These tissues play critical function for adaptation to variable stress conditions. The multilayered cortical tissue contains aerenchyma which are ...
... In rice roots, ground tissue (GT) is composed of one layer of exodermis, one layer of sclerenchyma, several layers of cortex and one layer of endodermis. These tissues play critical function for adaptation to variable stress conditions. The multilayered cortical tissue contains aerenchyma which are ...
Part 1
... Entropy helps in predicting the spontaneity of any process. An unfolded polypeptide chain has high entropy which goes on decreasing as the protein folds into its native state. 2. Free energy: The free energy, also known as Gibbs free energy, is the maximum amount of mechanical work that can be done ...
... Entropy helps in predicting the spontaneity of any process. An unfolded polypeptide chain has high entropy which goes on decreasing as the protein folds into its native state. 2. Free energy: The free energy, also known as Gibbs free energy, is the maximum amount of mechanical work that can be done ...
Gene Section BCL2L14 (BCL2-like 14 (apoptosis facilitator)) Atlas of Genetics and Cytogenetics
... several genes encoding pro-apoptotic Bcl-2 family members whose expression is markedly upregulated following shRNA-mediated depletion of Pdx1 in mouse insulinoma MIN6 cells (Fujimoto et al., 2010). Additionally BCL2L14 is one of several pro-apoptotic genes that is up-regulated upon exposure of human ...
... several genes encoding pro-apoptotic Bcl-2 family members whose expression is markedly upregulated following shRNA-mediated depletion of Pdx1 in mouse insulinoma MIN6 cells (Fujimoto et al., 2010). Additionally BCL2L14 is one of several pro-apoptotic genes that is up-regulated upon exposure of human ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.