- BioTek Instruments
... structural support to proteins acting as enzymes for modulating signal transduction pathways, such as kinases, proteases and phosphatases; to transmembrane proteins that allow for extracellular interactions, such as GPCRs and ion channels. Although almost all proteins are made from the same set of 2 ...
... structural support to proteins acting as enzymes for modulating signal transduction pathways, such as kinases, proteases and phosphatases; to transmembrane proteins that allow for extracellular interactions, such as GPCRs and ion channels. Although almost all proteins are made from the same set of 2 ...
Overview of tag protein fusions
... the target protein itself (e.g. stability, hydrophobicity), the expression system, and the application of the purified protein. This review provides an overview on the most frequently used and interesting tag-protein fusion systems (Table 2). ...
... the target protein itself (e.g. stability, hydrophobicity), the expression system, and the application of the purified protein. This review provides an overview on the most frequently used and interesting tag-protein fusion systems (Table 2). ...
The Copines, a Novel Class of C2 Domain-containing, Calcium
... flow-through fractions from this column contained the purified copine. Assay of the Lipid Binding and Aggregating Activities of Copine— Phospholipid binding activity of purified copine was determined by incubation of 5 mg of copine with 0.5–1 mg of phospholipid vesicles prepared as for the purificat ...
... flow-through fractions from this column contained the purified copine. Assay of the Lipid Binding and Aggregating Activities of Copine— Phospholipid binding activity of purified copine was determined by incubation of 5 mg of copine with 0.5–1 mg of phospholipid vesicles prepared as for the purificat ...
Gene Section DUSP1 (dual specificity phosphatase 1) Atlas of Genetics and Cytogenetics
... Mitogen activated protein kinases (MAPK) are a family of kinases that include the extracellular signal regulated kinases (ERKs), p38 and c-Jun NH2-terminal kinase (JNKs). They play an important role in regulation and are activated by a number of stimuli such as growth factors, cytokines or stress co ...
... Mitogen activated protein kinases (MAPK) are a family of kinases that include the extracellular signal regulated kinases (ERKs), p38 and c-Jun NH2-terminal kinase (JNKs). They play an important role in regulation and are activated by a number of stimuli such as growth factors, cytokines or stress co ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... different type and magnitude of selection pressure [13, 14]. For example, a buried position which maintains the protein’s configurational stability would be under strong selection; a surface residue with no functional role would be under no, or weak selection while a residue present on active-site i ...
... different type and magnitude of selection pressure [13, 14]. For example, a buried position which maintains the protein’s configurational stability would be under strong selection; a surface residue with no functional role would be under no, or weak selection while a residue present on active-site i ...
Results
... Elucidating these protein functions and linking their functionally related proteins improves our understanding of the mechanisms of biological systems at the molecular level [1]. As the number of sequenced genomes is increasing, conducting biological experiments to identify all protein pairs that ar ...
... Elucidating these protein functions and linking their functionally related proteins improves our understanding of the mechanisms of biological systems at the molecular level [1]. As the number of sequenced genomes is increasing, conducting biological experiments to identify all protein pairs that ar ...
A1980JB88800001
... been so often cited, I am beset with conflicting desires, to express irritation at the computerassisted trivialization of renown and to express pleasure at receiving an honor, however dubious. “The work came about as a consequence of a review, which I prepared for a few colleagues in 1957, of the th ...
... been so often cited, I am beset with conflicting desires, to express irritation at the computerassisted trivialization of renown and to express pleasure at receiving an honor, however dubious. “The work came about as a consequence of a review, which I prepared for a few colleagues in 1957, of the th ...
Improved topology prediction using the terminal
... separation between TM and non-TM proteins drastically. Using MSA, the overall accuracy increases to 74% without the prefilter and to 85% with the prefilter, but still only 43% of the proteins with long non-TM domains are predicted correctly. The overall performance of SCAMPI2 is 79–81% when using si ...
... separation between TM and non-TM proteins drastically. Using MSA, the overall accuracy increases to 74% without the prefilter and to 85% with the prefilter, but still only 43% of the proteins with long non-TM domains are predicted correctly. The overall performance of SCAMPI2 is 79–81% when using si ...
Gene expression
... coli to respond to its environment and switch its use of sugars. • Gene expression in bacteria was predicted to be triggered by specific signals from the environment. ...
... coli to respond to its environment and switch its use of sugars. • Gene expression in bacteria was predicted to be triggered by specific signals from the environment. ...
Trees from proteins I
... Proteins and amino acids • Proteins determine shape and structure of cells and carry most catalytic processes - 3D • Proteins are polymers of 20 different amino acids • Amino acids sequences determine the structure (2ndary, 3ary…) and function of the protein • Amino acids can be categorized by thei ...
... Proteins and amino acids • Proteins determine shape and structure of cells and carry most catalytic processes - 3D • Proteins are polymers of 20 different amino acids • Amino acids sequences determine the structure (2ndary, 3ary…) and function of the protein • Amino acids can be categorized by thei ...
Bending membranes
... Figure 1 Mechanisms to generate membrane curvature. (a) Local spontaneous membrane curvature generated by lipids. Lipids with a large area ratio of polar head groups to acyl chains create positive curvature; lipids with the opposite ratio create negative curvature (as does the insertion of cholester ...
... Figure 1 Mechanisms to generate membrane curvature. (a) Local spontaneous membrane curvature generated by lipids. Lipids with a large area ratio of polar head groups to acyl chains create positive curvature; lipids with the opposite ratio create negative curvature (as does the insertion of cholester ...
Leukaemia Section t(8;9)(p22;p24) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Reiter A, Walz C, Watmore A, Schoch C, Blau I, Schlegelberger B, Berger U, Telford N, Aruliah S, Yin JA, Vanstraelen D, Barker HF, Taylor PC, O'Driscoll A, Benedetti F, Rudolph C, Kolb HJ, Hochhaus A, Hehlmann R, Chase A, Cross NC. The t(8;9)(p22;p24) is a recurrent abnormality in chronic and acute ...
... Reiter A, Walz C, Watmore A, Schoch C, Blau I, Schlegelberger B, Berger U, Telford N, Aruliah S, Yin JA, Vanstraelen D, Barker HF, Taylor PC, O'Driscoll A, Benedetti F, Rudolph C, Kolb HJ, Hochhaus A, Hehlmann R, Chase A, Cross NC. The t(8;9)(p22;p24) is a recurrent abnormality in chronic and acute ...
Guide for Bioinformatics Project Module 3 - SGD-Wiki
... gene’s product in the cell based on its structural elements and similarity to structures of known proteins or protein domains by searching in several different databases. We will also look ...
... gene’s product in the cell based on its structural elements and similarity to structures of known proteins or protein domains by searching in several different databases. We will also look ...
Caulobacter Export™ Manual
... The LPS has been studied in some detail, in part because of a concern that it would act as an endotoxin in this protein expression system, much like the LPS of E. coli. Fortunately, Caulobacter has one of the rare forms of LPS that exhibits much lower endotoxin activity—at least 100-1000 fold lower ...
... The LPS has been studied in some detail, in part because of a concern that it would act as an endotoxin in this protein expression system, much like the LPS of E. coli. Fortunately, Caulobacter has one of the rare forms of LPS that exhibits much lower endotoxin activity—at least 100-1000 fold lower ...
References
... region targeted by P65-6 and upstream of the region targeted by P65-3b. Thus it was clear that we had amplified and cloned a cDNA fragment that encodes a large portion of the amino-terminal region of the Cd CA. Two nested non-degenerate primers CdCT-2 (5'- GGTCGACGTCGATCCTCAAGGC-3') and CdCT- 1 (5' ...
... region targeted by P65-6 and upstream of the region targeted by P65-3b. Thus it was clear that we had amplified and cloned a cDNA fragment that encodes a large portion of the amino-terminal region of the Cd CA. Two nested non-degenerate primers CdCT-2 (5'- GGTCGACGTCGATCCTCAAGGC-3') and CdCT- 1 (5' ...
sv-lncs - School of Mathematical and Computer Sciences
... account, by using the GO cellular component annotation, when calculating weighted cohesiveness, we are able to improve the results compared to previous work where only two of GO aspects (molecular function and biological process), were combined. We evaluate the predicted modules by calculating their ...
... account, by using the GO cellular component annotation, when calculating weighted cohesiveness, we are able to improve the results compared to previous work where only two of GO aspects (molecular function and biological process), were combined. We evaluate the predicted modules by calculating their ...
Identification of a gene cluster encoding an arginine ATP
... affinity (Kd=0?39±0?06 mM). Competition experiments revealed only partial inhibition by excess L-lysine (38 %) and L-ornithine (46 %), while no inhibition was observed with L-histidine or other amino acids tested. The membrane-associated transport complex, composed of a permease (designated ArtM) an ...
... affinity (Kd=0?39±0?06 mM). Competition experiments revealed only partial inhibition by excess L-lysine (38 %) and L-ornithine (46 %), while no inhibition was observed with L-histidine or other amino acids tested. The membrane-associated transport complex, composed of a permease (designated ArtM) an ...
Using the NCBI Genome Databases to Compare the
... acid sequence and, of course, in secondary, tertiary, and quaternary structhe genetic code is degenerate (more than one codon can code for the tures. In this activity, you will explore whether the gene that codes for same amino acid), two different genes can code for the same protein. They the beta ...
... acid sequence and, of course, in secondary, tertiary, and quaternary structhe genetic code is degenerate (more than one codon can code for the tures. In this activity, you will explore whether the gene that codes for same amino acid), two different genes can code for the same protein. They the beta ...
Impact of clostridial glucosylating toxins on the
... Background: The anaerobe Clostridium difficile produces two major virulence factors toxin A and B that inactivate Rho proteins by glucosylation of a pivotal threonine residue. Purified toxins induce reorganization of the cytoskeleton and cell death in colonic cells. Whether all toxin effects on targ ...
... Background: The anaerobe Clostridium difficile produces two major virulence factors toxin A and B that inactivate Rho proteins by glucosylation of a pivotal threonine residue. Purified toxins induce reorganization of the cytoskeleton and cell death in colonic cells. Whether all toxin effects on targ ...
1 Abstract
... on hydration. A 2D (Tricine-Tris) SDS-PAGE protein separation system was also used in attempts to isolate novel low molecular weight proteins from the pollen coats of both B. oleracea and A. thaliana. Here, results indicate that at least two of the four isoforms of the PCP-B family (PCP-Bβ and PCP-B ...
... on hydration. A 2D (Tricine-Tris) SDS-PAGE protein separation system was also used in attempts to isolate novel low molecular weight proteins from the pollen coats of both B. oleracea and A. thaliana. Here, results indicate that at least two of the four isoforms of the PCP-B family (PCP-Bβ and PCP-B ...
Bioinorganic motifs: towards functional classification of metalloproteins
... On the other hand, the field of bioinorganic chemistry is not confined to metalloproteins. Siderophores and antibiotics such as bleomycin are examples of naturally occurring non-protein metal-binding biological molecules (Lippard and Berg, 1994) which have their functional analogues in the protein w ...
... On the other hand, the field of bioinorganic chemistry is not confined to metalloproteins. Siderophores and antibiotics such as bleomycin are examples of naturally occurring non-protein metal-binding biological molecules (Lippard and Berg, 1994) which have their functional analogues in the protein w ...
294_2005_38_MOESM43_ESM - Springer Static Content Server
... Ptp2 and Ptp3: These two phosphotyrosine protein phosphatases are only 23% identical to each other and similarity is mainly focused on the C-terminal catalytic domain. Ptp2 and Ptp3 have partially redundant roles in S. cerevisiae as negative regulators of Hog1, but seem to perform their function in ...
... Ptp2 and Ptp3: These two phosphotyrosine protein phosphatases are only 23% identical to each other and similarity is mainly focused on the C-terminal catalytic domain. Ptp2 and Ptp3 have partially redundant roles in S. cerevisiae as negative regulators of Hog1, but seem to perform their function in ...
The Identity of Proteins Associated with a Small Heat Shock Protein
... (from 50 ml of heat-stressed cells) was separated by two-dimensional gel electrophoresis and stained with Coomassie Blue. Polypeptides identified as heat-associated with Hsp16.6 and released by DnaK, DnaJ, and GrpE were excised from the gel and subjected to trypsin digestion according to Shevchenko ...
... (from 50 ml of heat-stressed cells) was separated by two-dimensional gel electrophoresis and stained with Coomassie Blue. Polypeptides identified as heat-associated with Hsp16.6 and released by DnaK, DnaJ, and GrpE were excised from the gel and subjected to trypsin digestion according to Shevchenko ...
Supplementary method
... proteins. The peptide concentration was kept at 200 M and ATP concentration was 2 mM for Aurora specific activity assays. In microfluidic EphA3 assays, 100 ng of protein was evaluated in a kinetic experiment with 5 M EphA3 fluorescent peptide substrate (5-FAMEFPIYDFLPAKKK-CONH2). Phosphorylation w ...
... proteins. The peptide concentration was kept at 200 M and ATP concentration was 2 mM for Aurora specific activity assays. In microfluidic EphA3 assays, 100 ng of protein was evaluated in a kinetic experiment with 5 M EphA3 fluorescent peptide substrate (5-FAMEFPIYDFLPAKKK-CONH2). Phosphorylation w ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.