1 Biochemistry I: Proteins and Enzyme BIOC12H Summer 2015
... proteins and the important role proteins play as enzymes, signaling molecules and structural molecules in the cell. Throughout the term we will discuss the importance of protein folding and post-translational modifications as it relates to protein structure and function. At the end of this course Yo ...
... proteins and the important role proteins play as enzymes, signaling molecules and structural molecules in the cell. Throughout the term we will discuss the importance of protein folding and post-translational modifications as it relates to protein structure and function. At the end of this course Yo ...
The Protein Product of the c-cb! Protooncogene Is Phosphorylated
... To identify proteins that are associated with the Btk gene product, we produced the human Btk SH3 domain as a GST fusion protein (SH3-GST) and used it to immobilize polypeptide ligands from human B cell lysates. Metabolically radiolabeled Daudi cells were solubilized in lysis buffer and the clarifie ...
... To identify proteins that are associated with the Btk gene product, we produced the human Btk SH3 domain as a GST fusion protein (SH3-GST) and used it to immobilize polypeptide ligands from human B cell lysates. Metabolically radiolabeled Daudi cells were solubilized in lysis buffer and the clarifie ...
Protein Targeting into the Complex Plastid of Cryptophytes
... al. 2000). This cytoplasm, the periplastidal compartment, is devoid of typical eukaryotic compartments, e.g., mitochondrion and Golgi apparatus, but still harbors 80S ribosomes and a pigmy nucleus, the nucleomorph (Maier et al. 2000, Douglas et al. 2001; Cavalier-Smith 2002; Gilson and McFadden 2002 ...
... al. 2000). This cytoplasm, the periplastidal compartment, is devoid of typical eukaryotic compartments, e.g., mitochondrion and Golgi apparatus, but still harbors 80S ribosomes and a pigmy nucleus, the nucleomorph (Maier et al. 2000, Douglas et al. 2001; Cavalier-Smith 2002; Gilson and McFadden 2002 ...
The angiotensin-converting enzyme (ACE) gene family ofAnopheles
... is likely that ANCE-2, ANCE-3, ANCE-4 and ANCE-5 do not function as peptidases since they lack one or more of the residues that are essential for peptidase activity [15]. ACER, like ANCE and human ACE, is a peptidyl dipeptidase, but is generally less efficient than ANCE at cleaving dipeptides from m ...
... is likely that ANCE-2, ANCE-3, ANCE-4 and ANCE-5 do not function as peptidases since they lack one or more of the residues that are essential for peptidase activity [15]. ACER, like ANCE and human ACE, is a peptidyl dipeptidase, but is generally less efficient than ANCE at cleaving dipeptides from m ...
Nuclear Factor-90 of Activated T-Cells: A Double
... DNA-binding activity and transcriptional activation to the immunosuppressant drugs cyclosporin A and FK506 (31, 32). Both subunits of the heterodimeric complex have been cloned and found to consist of subunits of Mr ) 45 000 (NF45) and Mr ) 90 000 (NF90). The phosphorylation state of this ARRE-2 bin ...
... DNA-binding activity and transcriptional activation to the immunosuppressant drugs cyclosporin A and FK506 (31, 32). Both subunits of the heterodimeric complex have been cloned and found to consist of subunits of Mr ) 45 000 (NF45) and Mr ) 90 000 (NF90). The phosphorylation state of this ARRE-2 bin ...
Organization of the Kidney Proximal
... subunits, we can calculate that there are some 3800 molecules of actin/microvillus, amounting to 9.0% of the microvillus protein. We can therefore estimate the number of monomers of some of the other microvillus proteins (Table 1). The role of a-actinin (Mooseker & Tilney, 1975; Booth & Kenny, 1976~ ...
... subunits, we can calculate that there are some 3800 molecules of actin/microvillus, amounting to 9.0% of the microvillus protein. We can therefore estimate the number of monomers of some of the other microvillus proteins (Table 1). The role of a-actinin (Mooseker & Tilney, 1975; Booth & Kenny, 1976~ ...
Comparing DNA Sequences to Understand Evolutionary
... sequence of the genes in each of these species are available for anyone in the world to access via the Internet. Why is this information important? Being able to identify the precise location and sequence of human genes will allow us to better understand genetic diseases. In addition, learning about ...
... sequence of the genes in each of these species are available for anyone in the world to access via the Internet. Why is this information important? Being able to identify the precise location and sequence of human genes will allow us to better understand genetic diseases. In addition, learning about ...
SC.912.L.18.11 Explain the role of enzymes as catalysts that lower
... substance on which an enzyme acts during a chemical reaction is called a substrate. Enzymes act only on specific substrates. An enzyme’s shape determines its activity. Typically, an enzyme is a large protein with one or more deep folds on its surface. These folds form pockets called active sites. As ...
... substance on which an enzyme acts during a chemical reaction is called a substrate. Enzymes act only on specific substrates. An enzyme’s shape determines its activity. Typically, an enzyme is a large protein with one or more deep folds on its surface. These folds form pockets called active sites. As ...
The Genetic Code
... 1.Prepare a bottle of DNA extraction buer from 900 mL water, 50 mL dish detergent, and two teaspoons of table salt. Mix by inversion (cap it and turn it upside down a few times). 2.Grind a strawberry and a kiwifruit by hand in a plastic bag, or using a mortar and pestle, or with a metal bowl and th ...
... 1.Prepare a bottle of DNA extraction buer from 900 mL water, 50 mL dish detergent, and two teaspoons of table salt. Mix by inversion (cap it and turn it upside down a few times). 2.Grind a strawberry and a kiwifruit by hand in a plastic bag, or using a mortar and pestle, or with a metal bowl and th ...
amp activated protein kinase-á2 protects heart from left ventricular
... synthesis. Activation of AMPK attenuates cardiomyocyte hypertrophy. However, the relative physiological role of AMPK-alpha1 and AMPK-alpha2 in the development of ventricular hypertrophy and congestive heart failure is not clear. We studied the response of AMPK-alpha2 KO mice and AMPK-alpha1 KO mice ...
... synthesis. Activation of AMPK attenuates cardiomyocyte hypertrophy. However, the relative physiological role of AMPK-alpha1 and AMPK-alpha2 in the development of ventricular hypertrophy and congestive heart failure is not clear. We studied the response of AMPK-alpha2 KO mice and AMPK-alpha1 KO mice ...
Diversity and Formation of Endoplasmic Reticulum
... et al., 2001). All the compartments that have been characterized share three common features: (1) The ER-derived compartments accumulate a large amount of a single protein or only a few different proteins. (2) The protein components do not act within the compartments, even if they are functional pro ...
... et al., 2001). All the compartments that have been characterized share three common features: (1) The ER-derived compartments accumulate a large amount of a single protein or only a few different proteins. (2) The protein components do not act within the compartments, even if they are functional pro ...
Supplementary Report 18 August 2005
... group (good genes), and vice versa for genes with positive statistics. FDR = False ...
... group (good genes), and vice versa for genes with positive statistics. FDR = False ...
Slide 1
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
Ribosomes and Proteins
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
Membranes and Cell Transport
... Most Signal Transduction Pathways use G Proteins Three stages of Signal Transduction: Reception – each target cell has membrane receptors that detect a specific signal molecule and binds to it Transduction – binding of the signal molecule changes the receptor protein in some way that initiates trans ...
... Most Signal Transduction Pathways use G Proteins Three stages of Signal Transduction: Reception – each target cell has membrane receptors that detect a specific signal molecule and binds to it Transduction – binding of the signal molecule changes the receptor protein in some way that initiates trans ...
What is so memorable about CREBBP?
... CREB turns on other genes to store in long term memory. Switched on to create new connections between nerve cells Turning off and on genes by the act of remembering. In other words, CREB is a gene that mediates long-term memory. It acts as a master gene that turns on other genes, assisted by CREBBP ...
... CREB turns on other genes to store in long term memory. Switched on to create new connections between nerve cells Turning off and on genes by the act of remembering. In other words, CREB is a gene that mediates long-term memory. It acts as a master gene that turns on other genes, assisted by CREBBP ...
The role of the tumour suppressor p33ING1b in human neoplasia
... first to be identified and later isolated using an approach to detect genes whose expression is suppressed in cancer. The others were isolated through homology and similarity searches in human and mouse databases. All members contain a plant homeodomain involved in macromolecule recognition. Apart f ...
... first to be identified and later isolated using an approach to detect genes whose expression is suppressed in cancer. The others were isolated through homology and similarity searches in human and mouse databases. All members contain a plant homeodomain involved in macromolecule recognition. Apart f ...
Automatically Extracting Structured Information from Biomedical Text University of Texas at Austin
... TI - Two potentially oncogenic cyclins, cyclin A and cyclin D1, share common properties of subunit configuration, tyrosine phosphorylation and physical association with the Rb protein AB - Originally identified as a ‘mitotic cyclin’, cyclin A exhibits properties of growth factor sensitivity, suscept ...
... TI - Two potentially oncogenic cyclins, cyclin A and cyclin D1, share common properties of subunit configuration, tyrosine phosphorylation and physical association with the Rb protein AB - Originally identified as a ‘mitotic cyclin’, cyclin A exhibits properties of growth factor sensitivity, suscept ...
The ts111 Mutation of Paramecium tetraurelia Affects a
... at 35 o C induces disassembly of the infraciliary lattice (ICL). In this study, we found that the ts111 mutation also results in significant abnormalities in the number and structure of contractile vacuole complexes (CVCs) and in their functioning at the restrictive temperature. In order to characte ...
... at 35 o C induces disassembly of the infraciliary lattice (ICL). In this study, we found that the ts111 mutation also results in significant abnormalities in the number and structure of contractile vacuole complexes (CVCs) and in their functioning at the restrictive temperature. In order to characte ...
Chemical constituents
... Buddhist Chi Hong Chi Lam Memorial College A.L. Bio. Notes (by Denise Wong) The Cell ...... Page 10 ...
... Buddhist Chi Hong Chi Lam Memorial College A.L. Bio. Notes (by Denise Wong) The Cell ...... Page 10 ...
Solid Tumour Section t(4;22)(q35;q12) in embryonal rhabdomyo-sarcoma (ERMS) Atlas of Genetics and Cytogenetics
... One may speculate that the N terminal transactivation domain of EWSR1 was fused to one of the DNA binding domains of DUX4 (either the domain amino acids 19-78, or the domain aa 94-153). ...
... One may speculate that the N terminal transactivation domain of EWSR1 was fused to one of the DNA binding domains of DUX4 (either the domain amino acids 19-78, or the domain aa 94-153). ...
Evolvement of LEM proteins as chromatin tethers at the nuclear
... can repress transcription [11,15], although this was not seen in all studies [23], and may also be influenced by the respective experimental set-up, such as the promoters used [24]. Although lamins and several INM proteins have been shown to bind DNA or chromatin in vitro [3,25], the role of these p ...
... can repress transcription [11,15], although this was not seen in all studies [23], and may also be influenced by the respective experimental set-up, such as the promoters used [24]. Although lamins and several INM proteins have been shown to bind DNA or chromatin in vitro [3,25], the role of these p ...
Jeopardy Biology 3 PowerPoint
... A canyon forms in the middle of a territory of a species of ground squirrel. Later, there are two separate species of squirrel on each side of the canyon. This isolation mechanism best explains what occurred. A – Temporal Isolation B – Geographical Isolation C – Behavioral Isolation D – Isocentric I ...
... A canyon forms in the middle of a territory of a species of ground squirrel. Later, there are two separate species of squirrel on each side of the canyon. This isolation mechanism best explains what occurred. A – Temporal Isolation B – Geographical Isolation C – Behavioral Isolation D – Isocentric I ...
ENZYME HOMEWORK 1. What are enzymes made of?
... Name: ___________________________________________ENZYME HOMEWORK ...
... Name: ___________________________________________ENZYME HOMEWORK ...
Identification of a novel protein encoded by third conserved gene
... a reverse correlation to that observed in the cytoplasmic fraction. We conclude that the intracellular localization of NWC protein is not restricted to the nucleus or to the cytoplasm. Considering the possibility that NWC protein functions as a transcriptional regulator (Hong et al., 2008), this las ...
... a reverse correlation to that observed in the cytoplasmic fraction. We conclude that the intracellular localization of NWC protein is not restricted to the nucleus or to the cytoplasm. Considering the possibility that NWC protein functions as a transcriptional regulator (Hong et al., 2008), this las ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.