Nucleolar translocalization of GRA10 of Toxoplasma gondii
... Tg378, and then by precipitating the immunocomplexes with protein A agarose. The immunoprecipitates were washed once with 1 ml RIPA buffer. Then, the beads were washed twice with 1 ml TBS and centrifuged at 7,500 g for 2 min. The beads were then suspended in SDS sample buffer containing 0.125 M DTT ...
... Tg378, and then by precipitating the immunocomplexes with protein A agarose. The immunoprecipitates were washed once with 1 ml RIPA buffer. Then, the beads were washed twice with 1 ml TBS and centrifuged at 7,500 g for 2 min. The beads were then suspended in SDS sample buffer containing 0.125 M DTT ...
Merry Christmas for Patients with Hemophilia B
... two patients received a threefold higher dose and achieved about 2.5% of normal activity, and two patients received a 10-fold higher dose and achieved about 7% of normal activity. Expression has been seen for over 6 months in all patients, and prophylactic use of factor concentrate has either been e ...
... two patients received a threefold higher dose and achieved about 2.5% of normal activity, and two patients received a 10-fold higher dose and achieved about 7% of normal activity. Expression has been seen for over 6 months in all patients, and prophylactic use of factor concentrate has either been e ...
Orthologs, paralogs and homology inference Where are we now?
... families, from all RefGenome species, plus 35 other “phylogenetically informative” species • Tree Curation Tool has been updated by Paul’s and Suzi’s groups in collaboration – Retrieves and displays tree, and UniProt information for each sequence – Displays OrthoMCL clustering results-- scalable to ...
... families, from all RefGenome species, plus 35 other “phylogenetically informative” species • Tree Curation Tool has been updated by Paul’s and Suzi’s groups in collaboration – Retrieves and displays tree, and UniProt information for each sequence – Displays OrthoMCL clustering results-- scalable to ...
HiPer® Affinity Chromatography Teaching Kit
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
HIV-1 and Ebola virus encode small peptide motifs that recruit
... Short sequence from Gag protein and EbVp40 are sufficient to bind Tsg101 .This strategy can be used in anti viral activity against HIV and Ebola by using small inhibitors ...
... Short sequence from Gag protein and EbVp40 are sufficient to bind Tsg101 .This strategy can be used in anti viral activity against HIV and Ebola by using small inhibitors ...
2006 bradley de novo
... proteins were 102 amino acids in length, which is similar to a number of natural four-helix bundles. Characterization of five sequences chosen arbitrarily from this second-generation library showed that all were substantially more stable than the parental protein #86 (15). In addition, most of them ...
... proteins were 102 amino acids in length, which is similar to a number of natural four-helix bundles. Characterization of five sequences chosen arbitrarily from this second-generation library showed that all were substantially more stable than the parental protein #86 (15). In addition, most of them ...
DNA mimicry by proteins - Biochemical Society Transactions
... method of control is the use of sequence-specific DNAbinding proteins which block access of another protein, such as RNA polymerase, to the same sequence. The binding affinity of these repressor proteins for their DNA target depends on the cellular environment. Where two such repressor proteins act ...
... method of control is the use of sequence-specific DNAbinding proteins which block access of another protein, such as RNA polymerase, to the same sequence. The binding affinity of these repressor proteins for their DNA target depends on the cellular environment. Where two such repressor proteins act ...
Document
... Several rhoptry proteins are injected into the host cell cytoplasm during invasion They accumulate in the host cell nucleus Interestingly, some of them are enzymes capable of changing the phosphorylation state of proteins (kinases & phosphatases) Their precise function remains to be determin ...
... Several rhoptry proteins are injected into the host cell cytoplasm during invasion They accumulate in the host cell nucleus Interestingly, some of them are enzymes capable of changing the phosphorylation state of proteins (kinases & phosphatases) Their precise function remains to be determin ...
The Genetic Code
... shown below. The region of sequence shown is from the transcriptional start site to the transcriptional stop site. The gene’s one small intron is shown for you in bold. 5’-CTACGTACTAGCTATTCCGATCTATACTCGATCTAGTCGCATTCCGATTCGATCGTAC-3’ 3’-GATGCATGATCGATAAGGCTAGATATGAGCTAGATCAGCGTAAGGCTAAGCTAGCATG-5’ ( ...
... shown below. The region of sequence shown is from the transcriptional start site to the transcriptional stop site. The gene’s one small intron is shown for you in bold. 5’-CTACGTACTAGCTATTCCGATCTATACTCGATCTAGTCGCATTCCGATTCGATCGTAC-3’ 3’-GATGCATGATCGATAAGGCTAGATATGAGCTAGATCAGCGTAAGGCTAAGCTAGCATG-5’ ( ...
lecture 7
... - prefoldin can stabilize an unfolded protein for subsequent folding by chaperonin (explanation in class) - range of proteins archaeal prefoldin stabilizes is considerable: 14-62 kDa Archaeal prefoldin (with 2 different subunits) may play a general role in protein folding whereas the eukaryotic ch ...
... - prefoldin can stabilize an unfolded protein for subsequent folding by chaperonin (explanation in class) - range of proteins archaeal prefoldin stabilizes is considerable: 14-62 kDa Archaeal prefoldin (with 2 different subunits) may play a general role in protein folding whereas the eukaryotic ch ...
Supplemental Material
... proteins are given to the left. B) Western analysis of shifted material. Gel shifts were obtained in duplicate under standard conditions (domains and linkers are given in the panel). One set of gelshifted materials was used directly for obtaining an autoradiogram (three left panels marked gel shift) ...
... proteins are given to the left. B) Western analysis of shifted material. Gel shifts were obtained in duplicate under standard conditions (domains and linkers are given in the panel). One set of gelshifted materials was used directly for obtaining an autoradiogram (three left panels marked gel shift) ...
Extent of Gene Duplication in the Genomes of
... of two nonhomologous proteins into the same family because of the shared domain alone. Second, deciding whether two proteins are homologous becomes difficult when their sequence identity is low. In this paper, we improved the criterion adopted by Rost (1999), which is only based on the sequence iden ...
... of two nonhomologous proteins into the same family because of the shared domain alone. Second, deciding whether two proteins are homologous becomes difficult when their sequence identity is low. In this paper, we improved the criterion adopted by Rost (1999), which is only based on the sequence iden ...
An eye on trafficking genes: identification of four eye color mutations
... Two eye color mutants, lightoid (Rab32) and claret, its putative GEF, have been shown to affect pigment granule morphology and autophagy; lightoid’s transcript has also been shown to be enriched in neurons. The human Rab32 participates in transport of enzymes involved in melanin production to the me ...
... Two eye color mutants, lightoid (Rab32) and claret, its putative GEF, have been shown to affect pigment granule morphology and autophagy; lightoid’s transcript has also been shown to be enriched in neurons. The human Rab32 participates in transport of enzymes involved in melanin production to the me ...
EnCor Biotechnology Inc
... in C. elegans as a gene involved in sex determination. Fox is an acronym of "Feminizing locus on X". The mammalian genome contains three genes homologous to C. elegans Fox-1, called Fox1, Fox2 and Fox3. The Fox-1 protein and mammalian homologues are all about 46kDa in size, each of which includes a ...
... in C. elegans as a gene involved in sex determination. Fox is an acronym of "Feminizing locus on X". The mammalian genome contains three genes homologous to C. elegans Fox-1, called Fox1, Fox2 and Fox3. The Fox-1 protein and mammalian homologues are all about 46kDa in size, each of which includes a ...
The Investigation of Primary Failure of Eruption Etiology
... Higher proportion of patients with CIII skeletal ...
... Higher proportion of patients with CIII skeletal ...
IN VITRO TRANSCRIPTION . TRANSLATION - UTH e
... E. coli Cell-Free System E. coli cell-free systems consist of a crude extract that is rich in endogenous mRNA. The extract is incubated during preparation so that this endogenous mRNA is translated and subsequently degraded. Because the levels of endogenous mRNA in the prepared lysate is low, the ex ...
... E. coli Cell-Free System E. coli cell-free systems consist of a crude extract that is rich in endogenous mRNA. The extract is incubated during preparation so that this endogenous mRNA is translated and subsequently degraded. Because the levels of endogenous mRNA in the prepared lysate is low, the ex ...
Recombinant DNA procedures for producing small antimicrobial
... expressed in yeast and purified (Reichhart et al., 1992). The only example of an antimicrobial cationic peptide to be expressed in bacteria is a scorpion insectotoxin (Pang et al., 1992). This peptide was expressed in E. coli, but due to improper processing, had extra aa at the N terminus, and no bi ...
... expressed in yeast and purified (Reichhart et al., 1992). The only example of an antimicrobial cationic peptide to be expressed in bacteria is a scorpion insectotoxin (Pang et al., 1992). This peptide was expressed in E. coli, but due to improper processing, had extra aa at the N terminus, and no bi ...
The Genetic Code
... 1.Prepare a bottle of DNA extraction buer from 900 mL water, 50 mL dish detergent, and two teaspoons of table salt. Mix by inversion (cap it and turn it upside down a few times). 2.Grind a strawberry and a kiwifruit by hand in a plastic bag, or using a mortar and pestle, or with a metal bowl and th ...
... 1.Prepare a bottle of DNA extraction buer from 900 mL water, 50 mL dish detergent, and two teaspoons of table salt. Mix by inversion (cap it and turn it upside down a few times). 2.Grind a strawberry and a kiwifruit by hand in a plastic bag, or using a mortar and pestle, or with a metal bowl and th ...
Control of Gene Expression
... Gene Regulation In addition to sugars like glucose and lactose E. coli cells also require ...
... Gene Regulation In addition to sugars like glucose and lactose E. coli cells also require ...
Aipotu Part III: Molecular Biology
... • In the DNA sequence: o Promoters are shown in green o Terminators are shown in red • In the pre-mRNA sequence: o Exons are shown in color o Introns are not colored • In the mature mRNA sequence: o Exons are colored as in the pre-mRNA o Start and stop codons are underlined The Gene Windows work lik ...
... • In the DNA sequence: o Promoters are shown in green o Terminators are shown in red • In the pre-mRNA sequence: o Exons are shown in color o Introns are not colored • In the mature mRNA sequence: o Exons are colored as in the pre-mRNA o Start and stop codons are underlined The Gene Windows work lik ...
09_chapter 4
... the packing material is suspended in buffer, the charged groups become loosely associated with ions of the opposite charge. Since the buffer contains NaCl that dissociates into Na+ and Cl-, the loosely bound ions are called mobile counterions ((Walker, J.M.; 2005) Ion exchange chromatography separat ...
... the packing material is suspended in buffer, the charged groups become loosely associated with ions of the opposite charge. Since the buffer contains NaCl that dissociates into Na+ and Cl-, the loosely bound ions are called mobile counterions ((Walker, J.M.; 2005) Ion exchange chromatography separat ...
Photosynthesis is the portal through which the energy of sunlight
... structure across species. Using sequence information for 62 psbS proteins in 44 species, we identified domains that are highly variable in amino acid composition. Since we can assume that these diverse psbS proteins are capable of causing quenching in their native species, the variable domains could ...
... structure across species. Using sequence information for 62 psbS proteins in 44 species, we identified domains that are highly variable in amino acid composition. Since we can assume that these diverse psbS proteins are capable of causing quenching in their native species, the variable domains could ...
Gene Annotation
... • Choose a gene of interest from Rice, A. thaliana or related characterised organism • Iden9fy gene homologs/orthologs in other species by homology based BLAST analysis • Obtain protein and nucleo9de sequences ...
... • Choose a gene of interest from Rice, A. thaliana or related characterised organism • Iden9fy gene homologs/orthologs in other species by homology based BLAST analysis • Obtain protein and nucleo9de sequences ...
Genetically encoded phenyl azide photochemistry drives
... protein domains and additional cofactors. As demonstrated here with the widely used autouorescent protein mCherry, the position within the protein's molecular structure is critical to how the potential photochemical pathway taken will ultimately impact function. Through incorporation of a single nc ...
... protein domains and additional cofactors. As demonstrated here with the widely used autouorescent protein mCherry, the position within the protein's molecular structure is critical to how the potential photochemical pathway taken will ultimately impact function. Through incorporation of a single nc ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.