uniprotkb-goa_aug2011
... • Most popular type of GO analysis • Determines which GO terms are more often associated with a specified list of genes/proteins compared with a control list or rest of genome • Many tools available to do this analysis • User must decide which is best for their analysis ...
... • Most popular type of GO analysis • Determines which GO terms are more often associated with a specified list of genes/proteins compared with a control list or rest of genome • Many tools available to do this analysis • User must decide which is best for their analysis ...
you can view a sample report here.
... MTHFR C677TBHMT-rs3797546 CT AG down enzymatic rs1801133 function, incraeasing CBS The Cystathione Beta-Synthase (CBS) enzyme pulls homocysteine hydrogen sulfide MTHFR A1298C is involved in converting 5-methylfolate (5MTHF) tototetrahydrofolate the need for folate MTHFR 191150T (H2S) glutathione, re ...
... MTHFR C677TBHMT-rs3797546 CT AG down enzymatic rs1801133 function, incraeasing CBS The Cystathione Beta-Synthase (CBS) enzyme pulls homocysteine hydrogen sulfide MTHFR A1298C is involved in converting 5-methylfolate (5MTHF) tototetrahydrofolate the need for folate MTHFR 191150T (H2S) glutathione, re ...
The Sec34/35 Golgi Transport Complex Is Related to the Exocyst
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
Loss of Albino3 leads to the specific depletion of the light
... and nearly 10-fold in dark-grown cells. The accumulation of photosystem I, the cytochrome b6f complex, and ATP synthase is not affected in the ac29 mutant. Mild solubilization of thylakoid membranes reveals that Alb3 forms two distinct complexes, a lower molecular mass complex of a size similar to L ...
... and nearly 10-fold in dark-grown cells. The accumulation of photosystem I, the cytochrome b6f complex, and ATP synthase is not affected in the ac29 mutant. Mild solubilization of thylakoid membranes reveals that Alb3 forms two distinct complexes, a lower molecular mass complex of a size similar to L ...
Practical database searching
... of noncoding frames (although this latter issue still arises in DNA-as-protein searches). In addition, amino acids have chemical characteristics that allow degrees of similarity to be assessed rather than simple recognition of identity or non-identity. For these reasons, DNA versus DNA comparison (u ...
... of noncoding frames (although this latter issue still arises in DNA-as-protein searches). In addition, amino acids have chemical characteristics that allow degrees of similarity to be assessed rather than simple recognition of identity or non-identity. For these reasons, DNA versus DNA comparison (u ...
投影片 1
... Strains containing either ski4-1 or ski7Δ in combination with dcp1-2 or dcp2-7 are not able to grow under conditions restrictive for the decapping defect ...
... Strains containing either ski4-1 or ski7Δ in combination with dcp1-2 or dcp2-7 are not able to grow under conditions restrictive for the decapping defect ...
Sure, some prions can cause diseases, but others are
... a prion determines its configuration and how the resulting structures differ at the atomic level. HHMI investigator David Eisenberg, a protein structure specialist at the University of California, Los Angeles, uses various types of x-ray crystallography techniques to study the structure of the amylo ...
... a prion determines its configuration and how the resulting structures differ at the atomic level. HHMI investigator David Eisenberg, a protein structure specialist at the University of California, Los Angeles, uses various types of x-ray crystallography techniques to study the structure of the amylo ...
8.5
... are made up of twenty types of amino acids. The mRNA message is read as a series of non-overlapping codons, a sequence of three nucleotides that code for an amino acid. Many amino acids are coded for by more than one codon. In general, codons that code for the same amino acid share the same first tw ...
... are made up of twenty types of amino acids. The mRNA message is read as a series of non-overlapping codons, a sequence of three nucleotides that code for an amino acid. Many amino acids are coded for by more than one codon. In general, codons that code for the same amino acid share the same first tw ...
supplementary figures
... readings facilitated the comparison of relative protein expression levels with solvent treated control (which was set as “1”). b. After 24 h, the mRNA was purified, reverse transcribed and then qPCR was performed. The expression levels of MMP1 were normalised to GAPDH. qPCR was performed in triplica ...
... readings facilitated the comparison of relative protein expression levels with solvent treated control (which was set as “1”). b. After 24 h, the mRNA was purified, reverse transcribed and then qPCR was performed. The expression levels of MMP1 were normalised to GAPDH. qPCR was performed in triplica ...
Yeast epsins contain an essential N‐terminal ENTH domain, bind
... identified yAP180A and yAP180B, yeast homologs of the mammalian AP180 clathrin assembly polypeptide. In addition, we found a weakly positive clone corresponding to open reading frame (ORF) YDL161w (Wendland and Emr, 1998). Another yeast gene, YLR206w, predicts a protein that is 66% similar to YDL161 ...
... identified yAP180A and yAP180B, yeast homologs of the mammalian AP180 clathrin assembly polypeptide. In addition, we found a weakly positive clone corresponding to open reading frame (ORF) YDL161w (Wendland and Emr, 1998). Another yeast gene, YLR206w, predicts a protein that is 66% similar to YDL161 ...
Chapter 15
... There are still more codons than anticodons, because different codons can sometimes pair with the same anticodon through flexibility in base pairing at the third position of the codon. ...
... There are still more codons than anticodons, because different codons can sometimes pair with the same anticodon through flexibility in base pairing at the third position of the codon. ...
Sorting the Fatty Acid Chaff from the Toxin Wheat, or is it All
... Success in identifying genes and enzymes that are involved in the biosynthesis of toxins by dinoflagellates has been limited thus far, despite considerable efforts by many groups. The chemical structures of dinoflagellate polyketides suggest that they are produced by modular type I PKS enzymes in so ...
... Success in identifying genes and enzymes that are involved in the biosynthesis of toxins by dinoflagellates has been limited thus far, despite considerable efforts by many groups. The chemical structures of dinoflagellate polyketides suggest that they are produced by modular type I PKS enzymes in so ...
The Guanine Nucleotide–Binding Switch in Three Dimensions
... Because affinity studies showed the -phosphate–P loop interaction to be the most important element for tight binding of nucleotide, structural disturbance of the P loop is most likely the major reason for the drastically decreased affinity. In all the presently known complex structures besides RanR ...
... Because affinity studies showed the -phosphate–P loop interaction to be the most important element for tight binding of nucleotide, structural disturbance of the P loop is most likely the major reason for the drastically decreased affinity. In all the presently known complex structures besides RanR ...
Using storage organelles for the accumulation and encapsulation of
... to each antibody chain, which usually leads to the protein being secreted to the apoplast, although an optional Cterminal KDEL or HDEL tetrapeptide will retrieve secreted proteins to the endoplasmic reticulum (ER) [24]. The precise yields of a recombinant antibody achieved with this strategy vary fr ...
... to each antibody chain, which usually leads to the protein being secreted to the apoplast, although an optional Cterminal KDEL or HDEL tetrapeptide will retrieve secreted proteins to the endoplasmic reticulum (ER) [24]. The precise yields of a recombinant antibody achieved with this strategy vary fr ...
Heat shock proteins: the search for functions.
... of various thiol oxidants and heavy metals on chicken embryo fibroblasts and noted the induction of proteins resembling the HSPs (40). A comparison of the HSP90 and kinaseassociated P90 by proteolytic digestion indicated the two were identical and this was confirmed when the P90 showed a positive re ...
... of various thiol oxidants and heavy metals on chicken embryo fibroblasts and noted the induction of proteins resembling the HSPs (40). A comparison of the HSP90 and kinaseassociated P90 by proteolytic digestion indicated the two were identical and this was confirmed when the P90 showed a positive re ...
- Wiley Online Library
... size of RecA or SSB. The fact that pathogens usually pack their virulence proteins with several functions led to the hypothesis that the VirE2 protein may have an additional function. Several experimental results now show this to be the case. As the VirE2 protein had been found in the membrane fract ...
... size of RecA or SSB. The fact that pathogens usually pack their virulence proteins with several functions led to the hypothesis that the VirE2 protein may have an additional function. Several experimental results now show this to be the case. As the VirE2 protein had been found in the membrane fract ...
Chapter3summary
... When a protein fails to fold correctly, serious complications can occur. In Alzheimer’s disease, misfolded proteins accumulate in the brain and bind to one another, forming fibers in the areas of the brain that control memory, mood, and spatial awareness. ...
... When a protein fails to fold correctly, serious complications can occur. In Alzheimer’s disease, misfolded proteins accumulate in the brain and bind to one another, forming fibers in the areas of the brain that control memory, mood, and spatial awareness. ...
Introduction - Cedar Crest College
... When a protein fails to fold correctly, serious complications can occur. In Alzheimer’s disease, misfolded proteins accumulate in the brain and bind to one another, forming fibers in the areas of the brain that control memory, mood, and spatial awareness. ...
... When a protein fails to fold correctly, serious complications can occur. In Alzheimer’s disease, misfolded proteins accumulate in the brain and bind to one another, forming fibers in the areas of the brain that control memory, mood, and spatial awareness. ...
Chapter 3: Lecture Notes
... • The amino acids present in living organisms all exist in the L-configuration. (see Figure 2.21.) • The amino acids formed in the Miller-Urey experiments were mixtures of the D- and L-forms. • Recent experiments show that rocks present in abundance during the Archean had unique crystal structures t ...
... • The amino acids present in living organisms all exist in the L-configuration. (see Figure 2.21.) • The amino acids formed in the Miller-Urey experiments were mixtures of the D- and L-forms. • Recent experiments show that rocks present in abundance during the Archean had unique crystal structures t ...
Targeting of interleukin-2 to the periplasm of
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
Unit 2 - Protein Synthesis AAB - bushelman-hap
... 1. A second tRNA bonds with the next three bases of the mRNA, the amino acid links onto the amino acid of the first tRNA via a peptide bond. (Reminder) Each tRNA specific for one amino acid only, but some amino acids coded for by up to 6 codons. Order of bases in mRNA codons determine which tRNA ant ...
... 1. A second tRNA bonds with the next three bases of the mRNA, the amino acid links onto the amino acid of the first tRNA via a peptide bond. (Reminder) Each tRNA specific for one amino acid only, but some amino acids coded for by up to 6 codons. Order of bases in mRNA codons determine which tRNA ant ...
by gene expression, and of
... patterns)—is highly conserved and has now been identified in proteins from a wide variety of organisms, including humans . The DNA-binding segment of the domain is related to the helix-turn-helix motif. The DNA sequence that encodes this domain is known as the homeobox. ...
... patterns)—is highly conserved and has now been identified in proteins from a wide variety of organisms, including humans . The DNA-binding segment of the domain is related to the helix-turn-helix motif. The DNA sequence that encodes this domain is known as the homeobox. ...
Lecture 17 Outline Cell Motility: Encompasses both changes in cell
... movement of cilia different but mechanism same. Key is axonemal dynein that can bind MT at head and tail. Cross bridges between the neighboring tubule pairs ( via Nexin protein) allows movement of ciliary dyneins to not cause sliding of one filament over other, instead, bending of cilia or flagella. ...
... movement of cilia different but mechanism same. Key is axonemal dynein that can bind MT at head and tail. Cross bridges between the neighboring tubule pairs ( via Nexin protein) allows movement of ciliary dyneins to not cause sliding of one filament over other, instead, bending of cilia or flagella. ...
CHAPTER 1 PATHOGENESIS OF GOUSIEKTE 1.1
... Titin, previously called connectin, is a giant macromolecule of 3.0-3.7 mDa, and it spans the length of half a sarcomere to form a third filament system in vertebrate striated muscle. Titin molecules run from the Z-line through the I-band and A-band to the M-band, thereby linking the different sarco ...
... Titin, previously called connectin, is a giant macromolecule of 3.0-3.7 mDa, and it spans the length of half a sarcomere to form a third filament system in vertebrate striated muscle. Titin molecules run from the Z-line through the I-band and A-band to the M-band, thereby linking the different sarco ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.