Regulation of Gene Expression
... Summary 28.3 Regulation of Gene expression in Eukaryotes Hormones affect the regulation of gene expression in one of two ways. Steroid hormones interact directly with intracellular receptors that are DNA-binding regulatory proteins; binding of the hormone has either positive or negative effects o ...
... Summary 28.3 Regulation of Gene expression in Eukaryotes Hormones affect the regulation of gene expression in one of two ways. Steroid hormones interact directly with intracellular receptors that are DNA-binding regulatory proteins; binding of the hormone has either positive or negative effects o ...
Assembly of AO and DHAS - Journal of Cell Science
... matrix (data not shown). Although AO appears to cross the observed for DHAS, although the kinetics of association with peroxisomal membrane more slowly than DHAS, our data do peroxisomes was considerably faster (Goodman, 1985). The not allow us to conclude that mechanistic differences exist fraction ...
... matrix (data not shown). Although AO appears to cross the observed for DHAS, although the kinetics of association with peroxisomal membrane more slowly than DHAS, our data do peroxisomes was considerably faster (Goodman, 1985). The not allow us to conclude that mechanistic differences exist fraction ...
-Chain Gene in Epididymis α Expression of the C4b
... cDNA library was screened with a 0.2-kb fragment of guinea pig C4BP␣, previously isolated by RT-PCR. These clones were divided into two groups according to the nucleotide sequences of their coding regions. Four of these clones encoded seven SCRs followed by the C-terminal region and were identified ...
... cDNA library was screened with a 0.2-kb fragment of guinea pig C4BP␣, previously isolated by RT-PCR. These clones were divided into two groups according to the nucleotide sequences of their coding regions. Four of these clones encoded seven SCRs followed by the C-terminal region and were identified ...
Fish Meal (Mina) - UMK CARNIVORES 3
... when properly processed, are usually not as digestible as fishmeal, and their inclusion rate into the diet is often limited as it results in depressed growth rates and feed intake. Over-all protein digestibility values for fishmeal are consistently above 95%. In comparison protein digestibility for ...
... when properly processed, are usually not as digestible as fishmeal, and their inclusion rate into the diet is often limited as it results in depressed growth rates and feed intake. Over-all protein digestibility values for fishmeal are consistently above 95%. In comparison protein digestibility for ...
Gene Section YAP1 (Yes-associated protein 1, 65kDa) Atlas of Genetics and Cytogenetics
... able to interact with SH3 domains of many other proteins. In addition Yap contains another binding domain of a different nature. Due to the presence of two tryptophan residues, which appear to be conserved along evolution and that play an important role in the domain structure and function, it was n ...
... able to interact with SH3 domains of many other proteins. In addition Yap contains another binding domain of a different nature. Due to the presence of two tryptophan residues, which appear to be conserved along evolution and that play an important role in the domain structure and function, it was n ...
Nucleolin: A Multifunctional Major Nucleolar Phosphoprotein
... it is present in abundance at the heart of the nucleolus. It is highly conserved during evolution. Nucleolin contains a specific bipartite nuclear localization signal sequence and possesses a number of unusual structural features. It has unique tripartite structure and each domain performs a specifi ...
... it is present in abundance at the heart of the nucleolus. It is highly conserved during evolution. Nucleolin contains a specific bipartite nuclear localization signal sequence and possesses a number of unusual structural features. It has unique tripartite structure and each domain performs a specifi ...
protein phosphorylation in bacteria
... In the catalytic cleft of these enzymes there is a conserved cysteine residue, which is crucial for enzymatic activity. Those enzymes have molecular mass of about 30-40 kDa. Second group, Low Molecular Weight tyrosine phosphatases (LMW PTP), consists of small molecules of molecular mass equal to mor ...
... In the catalytic cleft of these enzymes there is a conserved cysteine residue, which is crucial for enzymatic activity. Those enzymes have molecular mass of about 30-40 kDa. Second group, Low Molecular Weight tyrosine phosphatases (LMW PTP), consists of small molecules of molecular mass equal to mor ...
Thierry Vrain, Innisfree Farm – The GMO Apple
... In 1996 the toxicologists of the FDA predicted that engineered foods would contain rogue proteins that could be toxic, or cause allergies, nutritional deficiencies, and other diseases. Biotechnology 2004 ...
... In 1996 the toxicologists of the FDA predicted that engineered foods would contain rogue proteins that could be toxic, or cause allergies, nutritional deficiencies, and other diseases. Biotechnology 2004 ...
The Bacterial Phosphotransferase System: Structure, Function
... In 1964, the three recognized activities of the PTS were presumed to correspond merely to three proteins. We now recognize dozens of PTS proteins in E. coli as well as hundreds of PTS proteins in other bacteria. Numerous genes encoding these proteins have been fully sequences, and their phylogenetic ...
... In 1964, the three recognized activities of the PTS were presumed to correspond merely to three proteins. We now recognize dozens of PTS proteins in E. coli as well as hundreds of PTS proteins in other bacteria. Numerous genes encoding these proteins have been fully sequences, and their phylogenetic ...
Expression systems for industrial Gram
... was based on the E. coli facf repressor gene, equipped with an appropriate promoter and ribosome binding site in conjunction with t!re inducible Spat-1 promoter, consisting of the promoter of phage SPO-1 coupled to the fat operator [44]. This system, however, could only be used for specific, small-s ...
... was based on the E. coli facf repressor gene, equipped with an appropriate promoter and ribosome binding site in conjunction with t!re inducible Spat-1 promoter, consisting of the promoter of phage SPO-1 coupled to the fat operator [44]. This system, however, could only be used for specific, small-s ...
cytoplasm nucleus and the A specific subset of
... domain is required for shuttling. This domain, however, is not sufficient to promote shuttling of an unrelated protein reporter, suggesting that stable RNA binding mediated by the RNA-recognition motifs may be required for shuttling. Consistent with such a requirement, a double point-mutation in RRM ...
... domain is required for shuttling. This domain, however, is not sufficient to promote shuttling of an unrelated protein reporter, suggesting that stable RNA binding mediated by the RNA-recognition motifs may be required for shuttling. Consistent with such a requirement, a double point-mutation in RRM ...
University of Groningen Characterization of the lytic-lysogenic
... ORF76 at the amino acid level, binds the switch region from Tuc2009 (data not shown). In order to determine the sequences recognized by Cro2009 DNase I protection assays were performed on the DNA encoding the intergenic region in the presence of Cro2009 (Fig. 2D). Two protected regions were observed ...
... ORF76 at the amino acid level, binds the switch region from Tuc2009 (data not shown). In order to determine the sequences recognized by Cro2009 DNase I protection assays were performed on the DNA encoding the intergenic region in the presence of Cro2009 (Fig. 2D). Two protected regions were observed ...
Structural investigation of single biomolecules
... using the AFM, to gain information about both the normal protein structure and its failure modes. In the case of bacteriorhodopsin, the protein forms very highly packed structures in the bacterial cell wall, and so is one of the few membrane proteins that can be crystallized for highresolution struc ...
... using the AFM, to gain information about both the normal protein structure and its failure modes. In the case of bacteriorhodopsin, the protein forms very highly packed structures in the bacterial cell wall, and so is one of the few membrane proteins that can be crystallized for highresolution struc ...
Pleiotropic control of glucose and hormone responses by PRL1, a
... porter genes (SUC1; Sauer and Stolz 1994) was found to be active in prl1, but not in wild-type plants grown in the absence of cytokinin. The sucrose synthase (SUS1), alcohol dehydrogenase (ADH), anionic peroxidase (PERA), and peroxidase C (PERC) genes showed derepression in the absence and enhanced ...
... porter genes (SUC1; Sauer and Stolz 1994) was found to be active in prl1, but not in wild-type plants grown in the absence of cytokinin. The sucrose synthase (SUS1), alcohol dehydrogenase (ADH), anionic peroxidase (PERA), and peroxidase C (PERC) genes showed derepression in the absence and enhanced ...
Chapter 16: Gene Regulation in Bacteria
... worked out. Our initial understanding of gene regulation can be traced back to the 1950s and the creative minds of two French scientists: François Jacob and Jacques Monod. They were interested in the phenomenon of enzyme adaptation, which refers to the observation that a particular enzyme appears wi ...
... worked out. Our initial understanding of gene regulation can be traced back to the 1950s and the creative minds of two French scientists: François Jacob and Jacques Monod. They were interested in the phenomenon of enzyme adaptation, which refers to the observation that a particular enzyme appears wi ...
HRT Gene Function Requires Interaction between a NAC Protein
... Two other Arabidopsis members of the NAC family of proteins, ATAF1 and ATAF2, were found to activate the 35S promoter of cauliflower mosaic virus in yeast cells (cited by Souer et al. [1996] as a personal communication). That suggests these two proteins may be transcription factors, although their f ...
... Two other Arabidopsis members of the NAC family of proteins, ATAF1 and ATAF2, were found to activate the 35S promoter of cauliflower mosaic virus in yeast cells (cited by Souer et al. [1996] as a personal communication). That suggests these two proteins may be transcription factors, although their f ...
File
... A mutant has been isolated that produces all three enzymes at the same level as induced cells do, even without any yuctose in the medium. ...
... A mutant has been isolated that produces all three enzymes at the same level as induced cells do, even without any yuctose in the medium. ...
Full Text - Genes | Genomes | Genetics
... function of these proteins in establishing the occluding junction, whereas very few studies have examined further developmental roles for them. Here we examined embryos with mutations in nine different core SJ genes and found that all nine result in defects in embryonic development as early as germ ...
... function of these proteins in establishing the occluding junction, whereas very few studies have examined further developmental roles for them. Here we examined embryos with mutations in nine different core SJ genes and found that all nine result in defects in embryonic development as early as germ ...
European Journal of Biochemistry
... proteins all have general pore properties, it has recently been shown that PhoE protein forms particularly efficient channels for organic and inorganic phosphate [4] and other negatively charged solutes [5], (Korteland et al., unpublished). The structural genes for the three pore proteins, ompF, omp ...
... proteins all have general pore properties, it has recently been shown that PhoE protein forms particularly efficient channels for organic and inorganic phosphate [4] and other negatively charged solutes [5], (Korteland et al., unpublished). The structural genes for the three pore proteins, ompF, omp ...
Tackling Unwanted Proteolysis in Plant Production Hosts Used for
... BY-2 cells and other recombinant proteins, such as human α1 -antitrypsin or BSA, spiked into the intercellular washing fluid of tobacco plants was partially inhibited by the addition of PMSF (Delannoy et al., 2008; Navarre et al., 2012; Castilho et al., 2014). Because most pharmaceutical proteins ar ...
... BY-2 cells and other recombinant proteins, such as human α1 -antitrypsin or BSA, spiked into the intercellular washing fluid of tobacco plants was partially inhibited by the addition of PMSF (Delannoy et al., 2008; Navarre et al., 2012; Castilho et al., 2014). Because most pharmaceutical proteins ar ...
Recombinant DNA WS
... 3. Gene B (1450 bp) will be cut with Enzyme A and Enzyme B, and inserted into Plasmid P. a. How many pieces of plasmid will result from the cut? b. What is the length of each plasmid piece? c. The cut plasmid is run through a gel. Draw & label each band(s) on the resulting gel. d. What is the result ...
... 3. Gene B (1450 bp) will be cut with Enzyme A and Enzyme B, and inserted into Plasmid P. a. How many pieces of plasmid will result from the cut? b. What is the length of each plasmid piece? c. The cut plasmid is run through a gel. Draw & label each band(s) on the resulting gel. d. What is the result ...
Primary cilia and polycystic kidney disease
... Although it was speculated early on that polycystin-2 acts as an ion channel (Mochizuki et al., 1996), it took several years before formal proof was obtained. Meanwhile it has been firmly established that polycystin-2 belongs to the TRP family of cation channels. It is permeable for mono- and divale ...
... Although it was speculated early on that polycystin-2 acts as an ion channel (Mochizuki et al., 1996), it took several years before formal proof was obtained. Meanwhile it has been firmly established that polycystin-2 belongs to the TRP family of cation channels. It is permeable for mono- and divale ...
Objectives 23 - u.arizona.edu
... - enzymes are effective biological catalysts bring reactants together in optimal orientation - catalysts speed up reaction by stabilizing transition state between reactants and products lower activation energy for reaction; catalysts not consumed - enzymes are highly specific for substrates; one ...
... - enzymes are effective biological catalysts bring reactants together in optimal orientation - catalysts speed up reaction by stabilizing transition state between reactants and products lower activation energy for reaction; catalysts not consumed - enzymes are highly specific for substrates; one ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.