Universitat Autònoma de Barcelona
... 2. Aggregation of the neuroblastoma-associated mutant (S120G) of the human nucleoside diphosphate kinase-A/NM23-H1 into amyloid fibrils Georgescauld F, Sabaté R, Espargaró A, Ventura S, Chaignepain S, Lacombe ML, Lascu I Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Ba ...
... 2. Aggregation of the neuroblastoma-associated mutant (S120G) of the human nucleoside diphosphate kinase-A/NM23-H1 into amyloid fibrils Georgescauld F, Sabaté R, Espargaró A, Ventura S, Chaignepain S, Lacombe ML, Lascu I Naunyn Schmiedeberg’s Archives of Pharmacology, Oct 2011;384(4-5):373-381 3. Ba ...
Arabidopsis RTM1 and RTM2 Genes Function in Phloem to Restrict
... which is production of antifungal and insecticidal compounds (Bones and Rossiter, 1996). A JR protein from Maclura pomifera has direct insecticidal activity (Murdock et al., 1990). Thus, proteins with JRs function in plant defense but by mechanisms that appear distinct from virus resistance (Chishol ...
... which is production of antifungal and insecticidal compounds (Bones and Rossiter, 1996). A JR protein from Maclura pomifera has direct insecticidal activity (Murdock et al., 1990). Thus, proteins with JRs function in plant defense but by mechanisms that appear distinct from virus resistance (Chishol ...
LIINA JAKOBSON The roles of abscisic acid, CO , and the cuticle in
... the colonization of land by plants was an essential event for the contemporary ecosystems. This was accompanied by a sequence of evolutionary adaptations necessary for plants to survive in a dry environment. The pathways of stomatal regulation and cuticle biosynthesis evolved during the same era in ...
... the colonization of land by plants was an essential event for the contemporary ecosystems. This was accompanied by a sequence of evolutionary adaptations necessary for plants to survive in a dry environment. The pathways of stomatal regulation and cuticle biosynthesis evolved during the same era in ...
Caspary T, Larkins CE, Anderson KV. Dev Cell. 2007 May;12(5):767-78. The graded response to Sonic Hedgehog depends on cilia architecture.
... The Ciliary Axoneme Is Abnormal in hnn Mutants Because Arl13b localized to cilia, we examined the structure of the cilia in the node of e8.0 embryos, which are relatively long and easy to visualize. Scanning electron microscopy (SEM) showed that hnn nodal cilia were approximately half the length of ...
... The Ciliary Axoneme Is Abnormal in hnn Mutants Because Arl13b localized to cilia, we examined the structure of the cilia in the node of e8.0 embryos, which are relatively long and easy to visualize. Scanning electron microscopy (SEM) showed that hnn nodal cilia were approximately half the length of ...
-ATPases:
... same subfamily (I) and are both localized to the phloem (see below) where they may have the same function. Whether this reflects the general picture, however, is still too early to say. Another important question is: Do the various members of the H+-ATPase family in a given species have different ca ...
... same subfamily (I) and are both localized to the phloem (see below) where they may have the same function. Whether this reflects the general picture, however, is still too early to say. Another important question is: Do the various members of the H+-ATPase family in a given species have different ca ...
Pseudomonas aeruginosa Anaerobic Respiration in Biofilms
... viability on glass coverslips using confocal laser scanning microscopy (Figure 2D, parts A [top view] and B, [sagittal view]). Consistent with the results discussed above, isogenic pilA and fliC mutants formed very poor anaerobic biofilms relative to that formed by wild-type bacteria. In contrast, l ...
... viability on glass coverslips using confocal laser scanning microscopy (Figure 2D, parts A [top view] and B, [sagittal view]). Consistent with the results discussed above, isogenic pilA and fliC mutants formed very poor anaerobic biofilms relative to that formed by wild-type bacteria. In contrast, l ...
Salmonella pathogenicity island 1 (SPI-1) type III
... sufficient to mediate secretion through its cognate T3SS, requiring a secondary NSS identified as the chaperonebinding domain (CBD). The CBD is located within the first ~140 amino acids of some secreted proteins and harbours the binding site for the cognate chaperone, which is generally required for ...
... sufficient to mediate secretion through its cognate T3SS, requiring a secondary NSS identified as the chaperonebinding domain (CBD). The CBD is located within the first ~140 amino acids of some secreted proteins and harbours the binding site for the cognate chaperone, which is generally required for ...
Intermediary Metabolism and Life-History Trade
... focusing on the differential allocation of internal resources to competing life-history traits (Tinkle and Hadley 1975; Pianka 1981; Boggs 1997a; Rose and Bradley 1998; Zera and Harshman 2001; Zhao and Zera 2002). However, in vivo mechanisms of differential allocation remain poorly understood at the ...
... focusing on the differential allocation of internal resources to competing life-history traits (Tinkle and Hadley 1975; Pianka 1981; Boggs 1997a; Rose and Bradley 1998; Zera and Harshman 2001; Zhao and Zera 2002). However, in vivo mechanisms of differential allocation remain poorly understood at the ...
Conservation of PHO pathway in ascomycetes and the role of Pho84
... Persson et al. 1999) (figure 1). These transporters have 12 transmembrane domains with a long N-terminal SPX domain (Syg1-Pho81-Xpr1), a domain that is common to many PHO genes. Earlier, Pho91 was also considered as a low-affinity transport system but it is localized onto the vacuoles and, thus, not ...
... Persson et al. 1999) (figure 1). These transporters have 12 transmembrane domains with a long N-terminal SPX domain (Syg1-Pho81-Xpr1), a domain that is common to many PHO genes. Earlier, Pho91 was also considered as a low-affinity transport system but it is localized onto the vacuoles and, thus, not ...
Autophagy at the crossroads of catabolism and anabolism
... specific cargoes such as mitochondria, peroxisomes and ribosomes, and protein aggregates. Selective autophagy is mediated by autophagy cargo receptors that bind cargo earmarked with degradation signals, most commonly ubiquitin in mammals, through their ubiquitin-binding domain (UBD). These receptors ...
... specific cargoes such as mitochondria, peroxisomes and ribosomes, and protein aggregates. Selective autophagy is mediated by autophagy cargo receptors that bind cargo earmarked with degradation signals, most commonly ubiquitin in mammals, through their ubiquitin-binding domain (UBD). These receptors ...
Nuclear export signal located within the DNAbinding domain of the
... To investigate whether CRM1 was the shuttling receptor responsible for mediating STAT1 export, we performed a similar experiment in the presence of the speci®c CRM1 inhibitor, leptomycin B (Figure 2A, right panels). Leptomycin B binds CRM1 speci®cally and inhibits its export activity (Wolff et al., ...
... To investigate whether CRM1 was the shuttling receptor responsible for mediating STAT1 export, we performed a similar experiment in the presence of the speci®c CRM1 inhibitor, leptomycin B (Figure 2A, right panels). Leptomycin B binds CRM1 speci®cally and inhibits its export activity (Wolff et al., ...
The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic
... milliliters of the cell-free reaction volumes [33,37,38]. However, EcMraY was found to form aggregates in the presence of a range of detergents, and any purified material was inactive [38]. The cell-free expression system was useful for investigating the role of lipid composition on EcMraY function. ...
... milliliters of the cell-free reaction volumes [33,37,38]. However, EcMraY was found to form aggregates in the presence of a range of detergents, and any purified material was inactive [38]. The cell-free expression system was useful for investigating the role of lipid composition on EcMraY function. ...
Tetrazine−trans-cyclooctene Mediated Conjugation of Antibodies to
... promising components for developing nanoscale devices1,2 due to their small size, energy efficiency,3 and detection sensitivity.4,5 Recently, first proof-of-principle devices employing kinesin-1 and microtubules (MTs) have been fabricated to transport, sort, detect and manipulate cargo.6−16 In these ex ...
... promising components for developing nanoscale devices1,2 due to their small size, energy efficiency,3 and detection sensitivity.4,5 Recently, first proof-of-principle devices employing kinesin-1 and microtubules (MTs) have been fabricated to transport, sort, detect and manipulate cargo.6−16 In these ex ...
curriculum vitae- michael hippler
... and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many proteins of the fermentative metabolism. Comparative quantitative analyses were performed with the chlor ...
... and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many proteins of the fermentative metabolism. Comparative quantitative analyses were performed with the chlor ...
Robust methods for purification of histones from cultured
... Post-translational modifications (PTMs) of histones play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these pro ...
... Post-translational modifications (PTMs) of histones play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these pro ...
REVIEWS - Haseloff Lab
... Box 1 | Ubiquitin and the ubiquitin–26S proteasome system In the initial ATP-consuming reaction, an E1 (or ubiquitin-activating enzyme) first activates the ubiquitin moiety by forming a high energy thioester bond between an E1 Cys residue and the carboxy-terminal Gly of ubiquitin (see the figure, pa ...
... Box 1 | Ubiquitin and the ubiquitin–26S proteasome system In the initial ATP-consuming reaction, an E1 (or ubiquitin-activating enzyme) first activates the ubiquitin moiety by forming a high energy thioester bond between an E1 Cys residue and the carboxy-terminal Gly of ubiquitin (see the figure, pa ...
Transport and Sorting of the Solanum tuberosum Sucrose
... substrate concentration gradient by transporting sucrose in symport with a proton (Boorer et al., 1996). Protein–protein interactions play an important role in the function and regulation of proteins in general. The function of many bacterial and mammalian membrane transport proteins is coupled to t ...
... substrate concentration gradient by transporting sucrose in symport with a proton (Boorer et al., 1996). Protein–protein interactions play an important role in the function and regulation of proteins in general. The function of many bacterial and mammalian membrane transport proteins is coupled to t ...
Intraflagellar transport and the generation of dynamic, structurally
... Figure 1. Canonical motile and non-motile ciliary ultrastructures and different cilia morphologies. (a) Ubiquitous basal body-ciliary ultrastructures. The basal body region is normally built from triplet microtubules, labeled A, B C. Transitional fibers, which emanate from the distal end of the basa ...
... Figure 1. Canonical motile and non-motile ciliary ultrastructures and different cilia morphologies. (a) Ubiquitous basal body-ciliary ultrastructures. The basal body region is normally built from triplet microtubules, labeled A, B C. Transitional fibers, which emanate from the distal end of the basa ...
Nuclear-fallout, a Drosophila protein that cycles from the cytoplasm
... embryos, the network of metaphase furrows exhibit extensive gaps (Fig. 2F). These gaps often occur in regions of the furrow that are in line with the metaphase plate (Fig. 2F, arrows). As revealed by embryos double stained for microfilaments and centrosomes (Heuer et al., 1995; Li and Kaufman, 1996) ...
... embryos, the network of metaphase furrows exhibit extensive gaps (Fig. 2F). These gaps often occur in regions of the furrow that are in line with the metaphase plate (Fig. 2F, arrows). As revealed by embryos double stained for microfilaments and centrosomes (Heuer et al., 1995; Li and Kaufman, 1996) ...
Hereditary spastic paraplegia: clinical features and pathogenetic
... form of HSP with a variable age of onset.42 It is relatively common, and mutations in the REEP1 gene have been identified in 3% of a sample of unrelated patients with HSP, which increased to 8·2% in pure HSP if those with SPG3A and SPAST mutations were excluded.59 A base change in ZFYVE27 (SPG33) was ...
... form of HSP with a variable age of onset.42 It is relatively common, and mutations in the REEP1 gene have been identified in 3% of a sample of unrelated patients with HSP, which increased to 8·2% in pure HSP if those with SPG3A and SPAST mutations were excluded.59 A base change in ZFYVE27 (SPG33) was ...
The neomuran origin of archaebacteria, the negibacterial root of the
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
The neomuran origin of archaebacteria, the negibacterial root of the
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
The neomuran origin of archaebacteria, the
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
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... one-cell embryo (Tsai and Ahringer, 2007; Cowan and Hyman, 2004b; Sonneville and Gönczy, 2004). Microtubules from the meitoic spindle can also induce polarization in the absence of centrosomes in embryos arrested in metaphase of meiosis I (Wallenfang and Seydoux, 2000). However, during embryogenesis ...
... one-cell embryo (Tsai and Ahringer, 2007; Cowan and Hyman, 2004b; Sonneville and Gönczy, 2004). Microtubules from the meitoic spindle can also induce polarization in the absence of centrosomes in embryos arrested in metaphase of meiosis I (Wallenfang and Seydoux, 2000). However, during embryogenesis ...
A Role for A-to-I RNA Editing in Temperature Adaptation
... there are five closely spaced editing sites in sequence that encode the intracellular loop that couples the receptor with its G protein (9). The fully edited form reduces the stability of the interaction between the receptor and G protein (45). In vertebrate GABAA receptors, one of the primary media ...
... there are five closely spaced editing sites in sequence that encode the intracellular loop that couples the receptor with its G protein (9). The fully edited form reduces the stability of the interaction between the receptor and G protein (45). In vertebrate GABAA receptors, one of the primary media ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.