Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Clones for Expressing Bacterial Proteins–-The GST-Hairy fusion proteins bHLH, Orange-WRPW, N30 (an N-terminal 30-amino acid fragment of Hairy), Basic (basic DNA-binding elements), and HLH have been described previously (5, 16). Dmp53 (obtained from M. Brodsky) is a BamHI/BglII fragment inserted in t ...
... Clones for Expressing Bacterial Proteins–-The GST-Hairy fusion proteins bHLH, Orange-WRPW, N30 (an N-terminal 30-amino acid fragment of Hairy), Basic (basic DNA-binding elements), and HLH have been described previously (5, 16). Dmp53 (obtained from M. Brodsky) is a BamHI/BglII fragment inserted in t ...
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... growth and development [9], the vacuolar PR1-mCherry signal appeared much stronger. A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ...
... growth and development [9], the vacuolar PR1-mCherry signal appeared much stronger. A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ...
ASMS 2004 de Novo
... • It is demonstrated that fragmentations of charged TMPP-Ac derivatives follow different pathways under low energy CID performed in a Q-TOF mass spectrometer. ...
... • It is demonstrated that fragmentations of charged TMPP-Ac derivatives follow different pathways under low energy CID performed in a Q-TOF mass spectrometer. ...
A Raman spectroscopic study of the interaction between nucleotides
... polynucleotide-protein complexes the nucleotide shields the 4-6 tyrosine residues from coordination by the COO- groups even at high protein concentration. The presence of the nucleotide causes no shielding of the SH groups. With Raman difference spectroscopy i t is shown that binding of the protein ...
... polynucleotide-protein complexes the nucleotide shields the 4-6 tyrosine residues from coordination by the COO- groups even at high protein concentration. The presence of the nucleotide causes no shielding of the SH groups. With Raman difference spectroscopy i t is shown that binding of the protein ...
RELIC – A bioinformatics server for combinatorial
... data bank (PDB) file). The combined use of these three programs allows a user to determine whether any of the peptides obtained via affinity selection are mimicking one or more epitopes within a protein structure; facilitates the visualization of those mimicked epitopes within that protein structure ...
... data bank (PDB) file). The combined use of these three programs allows a user to determine whether any of the peptides obtained via affinity selection are mimicking one or more epitopes within a protein structure; facilitates the visualization of those mimicked epitopes within that protein structure ...
Biochemistry - Brookwood High School
... ex: oxygen gas, water, glucose • molecular formula – uses atomic symbols to represent atoms bound together in a compound ex: O2, H2O, C6H12O6 ...
... ex: oxygen gas, water, glucose • molecular formula – uses atomic symbols to represent atoms bound together in a compound ex: O2, H2O, C6H12O6 ...
Physical and chemical interactions between aphids and plants
... most likely candidates for fast plugging events. There is a variety of protein forms in the sieve tubes. Presumably most of the phloem-specific proteins are in a soluble form in the phloem sap, a few are present as insoluble deposits along the SE plasma membrane of dicotyledons, while again others a ...
... most likely candidates for fast plugging events. There is a variety of protein forms in the sieve tubes. Presumably most of the phloem-specific proteins are in a soluble form in the phloem sap, a few are present as insoluble deposits along the SE plasma membrane of dicotyledons, while again others a ...
The Other Lives of Ribosomal Proteins - PDXScholar
... Cellular mRNAs are produced in the nucleus and exported to the cytoplasm through nuclear pore complexes (NPC) that are found in the nuclear membrane. The correctly processed mRNAs are exported in the form of messenger ribonucleoproteins (mRNPs). Since the NPC allows the passage of only one mRNA mole ...
... Cellular mRNAs are produced in the nucleus and exported to the cytoplasm through nuclear pore complexes (NPC) that are found in the nuclear membrane. The correctly processed mRNAs are exported in the form of messenger ribonucleoproteins (mRNPs). Since the NPC allows the passage of only one mRNA mole ...
A model for mis-sense error in protein synthesis: mis
... is also the correct one, as directed by the corresponding template; the other end of the same cognate tRNA molecule, referred to as anti-codon, matches perfectly, by complementary base pairing, with the codon on the template mRNA. In contrast, increasing degree of mismatch makes the tRNA near-cognat ...
... is also the correct one, as directed by the corresponding template; the other end of the same cognate tRNA molecule, referred to as anti-codon, matches perfectly, by complementary base pairing, with the codon on the template mRNA. In contrast, increasing degree of mismatch makes the tRNA near-cognat ...
ref. #28 of the TIBS article
... structural and positional changes in a TMH. An obvious advantage is that the experiments are performed under in vivo-like conditions; the price one pays is that the complexity of the experimental system makes direct structural interpretations of the data dif®cult. However, we have shown here that th ...
... structural and positional changes in a TMH. An obvious advantage is that the experiments are performed under in vivo-like conditions; the price one pays is that the complexity of the experimental system makes direct structural interpretations of the data dif®cult. However, we have shown here that th ...
the structure and function of cartilage proteoglycans
... G3 (Fig. 1), each containing cysteine residues that participate in disulphide bond formation (Sandy et al., 1990). The G1 and G2 regions are separated by a short interglobular domain (IGD), and the G2 and G3 regions are separated by a long glycosaminoglycan (GAG)attachment region, which consists of ...
... G3 (Fig. 1), each containing cysteine residues that participate in disulphide bond formation (Sandy et al., 1990). The G1 and G2 regions are separated by a short interglobular domain (IGD), and the G2 and G3 regions are separated by a long glycosaminoglycan (GAG)attachment region, which consists of ...
Sample pages 1 PDF
... the panel of human ribosomes, the yeast 80S structure is shown in grayscale, and dashed lines indicate the positions of long RNA expansion segments, which are the most distinctive feature of ribosomes from higher eukaryotes ...
... the panel of human ribosomes, the yeast 80S structure is shown in grayscale, and dashed lines indicate the positions of long RNA expansion segments, which are the most distinctive feature of ribosomes from higher eukaryotes ...
Experimental Analysis of the Rice Mitochondrial
... MS), and the identification of 122 nonredundant rice mitochondrial proteins (Heazlewood et al., 2003). Subsequently, a separate set of 112 nonredundant rice mitochondrial proteins was identified and listed in the rice proteome database (Komatsu, 2005) using mitochondria isolated by Suc gradient cent ...
... MS), and the identification of 122 nonredundant rice mitochondrial proteins (Heazlewood et al., 2003). Subsequently, a separate set of 112 nonredundant rice mitochondrial proteins was identified and listed in the rice proteome database (Komatsu, 2005) using mitochondria isolated by Suc gradient cent ...
Cytosolic Hsp70 and co-chaperones constitute a novel system for
... eLife digest Plants, animals, and fungi all store their DNA inside their cells within a structure called the nucleus, which is surrounded by a nuclear envelope that separates it from the rest of the cell. This DNA contains the instructions to build proteins, but proteins are actually built elsewhere ...
... eLife digest Plants, animals, and fungi all store their DNA inside their cells within a structure called the nucleus, which is surrounded by a nuclear envelope that separates it from the rest of the cell. This DNA contains the instructions to build proteins, but proteins are actually built elsewhere ...
New Reactions in the Crotonase Superfamily: Structure of
... II and III as well. For the refinement of the apo-MMCD model, 10% of the X-ray data were excluded for the required calculation of Rfree as listed in Table 2. In that all X-ray data are important for the Fourier synthesis, however, these data were ultimately included in the final stages of the refine ...
... II and III as well. For the refinement of the apo-MMCD model, 10% of the X-ray data were excluded for the required calculation of Rfree as listed in Table 2. In that all X-ray data are important for the Fourier synthesis, however, these data were ultimately included in the final stages of the refine ...
Electron microscopy in structural studies of Photosystem II
... hydrophobic plane of the lipid bilayer to produce two complementary fractured faces. Since the integral protein complexes that span the membrane bilayer are not splitted during the fracturing process, they are seen as ‘particles’ that rise above a smooth surface. In freeze-etching studies, fracture ...
... hydrophobic plane of the lipid bilayer to produce two complementary fractured faces. Since the integral protein complexes that span the membrane bilayer are not splitted during the fracturing process, they are seen as ‘particles’ that rise above a smooth surface. In freeze-etching studies, fracture ...
Ciliary Microtubule Capping Structures Contain A
... and Chlamydomonas flagella were stained with the affinitypurified 97-kD antibodies. None of the membrane proteins were stained with the antisera (data not shown). Immunofluorescence staining of Tetrahymena cilia was carried out using the affinity-purified 97-kD antibodies. The affinity-purified anti ...
... and Chlamydomonas flagella were stained with the affinitypurified 97-kD antibodies. None of the membrane proteins were stained with the antisera (data not shown). Immunofluorescence staining of Tetrahymena cilia was carried out using the affinity-purified 97-kD antibodies. The affinity-purified anti ...
The Three-Dimensional Structure of Aspergillus niger Pectin Lyase
... less than 3.9 Å, a hydrogen-acceptor distance of less than 2.5 Å, and associated angles greater than 90o. A hydrophobic contact was assumed to exist between two apolar residues if the carbon-carbon distance was less than 4.0 Å. Amino acids were assigned to secondary structural elements if they exhib ...
... less than 3.9 Å, a hydrogen-acceptor distance of less than 2.5 Å, and associated angles greater than 90o. A hydrophobic contact was assumed to exist between two apolar residues if the carbon-carbon distance was less than 4.0 Å. Amino acids were assigned to secondary structural elements if they exhib ...
Effects of macromolecular crowding on protein folding and
... an average molecular mass of ~70 kDa. In addition to these compounds, it was decided to use BSA and chicken albumin (ovalbumin) as crowding agents, in order to monitor any effects due to more specific interactions of these proteins with refolding lysozyme. The results are summarized in Figure 2A. It ...
... an average molecular mass of ~70 kDa. In addition to these compounds, it was decided to use BSA and chicken albumin (ovalbumin) as crowding agents, in order to monitor any effects due to more specific interactions of these proteins with refolding lysozyme. The results are summarized in Figure 2A. It ...
Amino Acids
... • Each fibrous protein exhibits special mechanical properties, resulting from unique structure, obtained by specific aa’s combined into regular 2º structural elements. • In contrast to globular proteins, whose shapes result from complex interactions b/w 2º, 3º, and sometimes 4º elements. ...
... • Each fibrous protein exhibits special mechanical properties, resulting from unique structure, obtained by specific aa’s combined into regular 2º structural elements. • In contrast to globular proteins, whose shapes result from complex interactions b/w 2º, 3º, and sometimes 4º elements. ...
SoyMilk - Soyfoods Association of North America
... whole soybeans that are cooked at varying temperatures and filtered yielding a protein-rich soy base that naturally consists of soy protein, oil, fiber, sugars, water, and bio-active compounds. This soy base may be combined with a sweetener (such as rice syrup or cane juice), some flavor, and a stabili ...
... whole soybeans that are cooked at varying temperatures and filtered yielding a protein-rich soy base that naturally consists of soy protein, oil, fiber, sugars, water, and bio-active compounds. This soy base may be combined with a sweetener (such as rice syrup or cane juice), some flavor, and a stabili ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.