Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... crystallin (Röll et al. 1995). Searching the protein databases with the B-crystallin sequence resulted in the identification of 65 significantly related proteins. An initial alignment and phylogenetic tree constructed from these sequences (not shown) confirmed that this superfamily of proteins, th ...
... crystallin (Röll et al. 1995). Searching the protein databases with the B-crystallin sequence resulted in the identification of 65 significantly related proteins. An initial alignment and phylogenetic tree constructed from these sequences (not shown) confirmed that this superfamily of proteins, th ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... The enzymes specifically aimed at detoxification of superoxide are SODs. The SODs are widely distributed enzymes, present in all kingdoms of life. The family of enzymes with common name SOD consists of three unrelated enzyme types with the ability to catalyze dismutation of superoxide anion into eit ...
... The enzymes specifically aimed at detoxification of superoxide are SODs. The SODs are widely distributed enzymes, present in all kingdoms of life. The family of enzymes with common name SOD consists of three unrelated enzyme types with the ability to catalyze dismutation of superoxide anion into eit ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... Epstein-Barr virus (EBV). NPC tumor cells express viral proteins, including BARF1, which is secreted and is considered to have oncogenic and immune-modulating properties. Recently, we found conserved mutations in the BARF1 gene in NPC isolates. This study describes the expression and purification of ...
... Epstein-Barr virus (EBV). NPC tumor cells express viral proteins, including BARF1, which is secreted and is considered to have oncogenic and immune-modulating properties. Recently, we found conserved mutations in the BARF1 gene in NPC isolates. This study describes the expression and purification of ...
Yeast
... obtained by using a convenient single-step procedure. Such separation was possible because of the solubility of the Cu–Zn SOD in chloroform, whereas most of accompanying proteins precipitated. It is thus possible that the reduction of almost 35·5% of the total enzyme units found in the crude extract ...
... obtained by using a convenient single-step procedure. Such separation was possible because of the solubility of the Cu–Zn SOD in chloroform, whereas most of accompanying proteins precipitated. It is thus possible that the reduction of almost 35·5% of the total enzyme units found in the crude extract ...
Stereochemistry and Mechanism of Reactions Catalyzed by
... and 2 IU of the fil protein mixed with 2-fold excess of the u protein of tryptophan synthetase from E. co& In order to minimize exchange of the o-hydrogen of tryptophan after it has been formed, the incubation was carried out at 37°C for only 20 min; under these conditions the yield was not more tha ...
... and 2 IU of the fil protein mixed with 2-fold excess of the u protein of tryptophan synthetase from E. co& In order to minimize exchange of the o-hydrogen of tryptophan after it has been formed, the incubation was carried out at 37°C for only 20 min; under these conditions the yield was not more tha ...
Extreme sweetness: protein glycosylation in archaea
... lower eukaryotes (for example, invertebrates and protists)9, variants of this oligomer — using the same N‑acetylglucosamine (GlcNAc)- and mannose-based core but lacking or including additional sugar residues from a limited roster at various positions — serve to expand the repertoire of N‑linked glyc ...
... lower eukaryotes (for example, invertebrates and protists)9, variants of this oligomer — using the same N‑acetylglucosamine (GlcNAc)- and mannose-based core but lacking or including additional sugar residues from a limited roster at various positions — serve to expand the repertoire of N‑linked glyc ...
Scientific Opinion on Dietary Reference Values for protein
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
The Case of Protein Kinase CK2
... The grouping of the protein kinases based on catalytic subunit sequence similarity results in clustering of kinases that share functional features, such as preferred sites of phosphorylation, the mode of regulation and cellular localization. The similarity in the amino acid sequence of the catalytic ...
... The grouping of the protein kinases based on catalytic subunit sequence similarity results in clustering of kinases that share functional features, such as preferred sites of phosphorylation, the mode of regulation and cellular localization. The similarity in the amino acid sequence of the catalytic ...
Protein Creation Pathway
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
Protein Creation Pathway
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
EP 1790660 B1
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
Herculex® I Product Overview
... Herculex® I insect protection for corn, co-developed by Dow AgroSciences and Pioneer Hi-Bred is a superior example of the use of a natural Bt protein. The Bt protein (Cry1F) produced in corn with the Herculex I trait offers the broadest spectrum of above-the-ground insect pest control on the market. ...
... Herculex® I insect protection for corn, co-developed by Dow AgroSciences and Pioneer Hi-Bred is a superior example of the use of a natural Bt protein. The Bt protein (Cry1F) produced in corn with the Herculex I trait offers the broadest spectrum of above-the-ground insect pest control on the market. ...
The Effect of Ultrasound on the Functional Properties of
... which in turn produce high shear energy waves and turbulence in the cavitation zone. The combination of pressure, heat and turbulence affects the ultrasounded liquid [4]. This is one aspect of the acoustic cavitation phenomenon. Another aspect is the development of strong micro-streaming currents re ...
... which in turn produce high shear energy waves and turbulence in the cavitation zone. The combination of pressure, heat and turbulence affects the ultrasounded liquid [4]. This is one aspect of the acoustic cavitation phenomenon. Another aspect is the development of strong micro-streaming currents re ...
Essays41 Chap03 - Essays in Biochemistry
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
... easily cover this distance by diffusion, and by use of these substrates it has been found that archaebacterial proteasomes preferentially hydrolyse peptide bonds at the C-terminal side of large hydrophobic amino-acid residues, thus exhibiting chymotrypsin-like specificity. However, within protein su ...
Chemistry 100 Second Homework
... This homework will not be turned in. It is to help you study for the final exam. The answer key will be provided Thursday. 1. Draw the structure of the addition polymer formed from each of these compounds. CH3 a. CCl2=CH2 b. H2C ...
... This homework will not be turned in. It is to help you study for the final exam. The answer key will be provided Thursday. 1. Draw the structure of the addition polymer formed from each of these compounds. CH3 a. CCl2=CH2 b. H2C ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... Understanding the how CesA proteins interact is of great importance to the development of a robust model of plant cellulose biosynthesis. In order to characterize the interaction between the CesA proteins by quantitative means, we have studied the in vitro interactions between Arabidopsis CesA N-ter ...
... Understanding the how CesA proteins interact is of great importance to the development of a robust model of plant cellulose biosynthesis. In order to characterize the interaction between the CesA proteins by quantitative means, we have studied the in vitro interactions between Arabidopsis CesA N-ter ...
Лекция 2. Структура и функция белка
... 10), sugars and polysaccharides (Chapter 11), and nucleotides and nucleic acids (Chapter 12). We begin in each case by considering the covalent structure of the simple subunits (amino acids, fatty acids, monosaccharides, and nucleotides). These subunits are a major part of the language of biochemist ...
... 10), sugars and polysaccharides (Chapter 11), and nucleotides and nucleic acids (Chapter 12). We begin in each case by considering the covalent structure of the simple subunits (amino acids, fatty acids, monosaccharides, and nucleotides). These subunits are a major part of the language of biochemist ...
Structural studies on lysosomal proteins
... received a Nobel prize of Physiology and Medicine for his findings in 1974. The first lysosomal storage disorder was described by H. G. Hers in 1963, when he reported the absence of lysosomal !-glucosidase in Pompe disease (Hers, 1963). In the case of the lysosome, the separation of the organelle’s ...
... received a Nobel prize of Physiology and Medicine for his findings in 1974. The first lysosomal storage disorder was described by H. G. Hers in 1963, when he reported the absence of lysosomal !-glucosidase in Pompe disease (Hers, 1963). In the case of the lysosome, the separation of the organelle’s ...
Protein metabolism in exercising human with special reference to
... tissue is being synthesized. This is often observed in children, during pregnancy, in recovery from illness, and during resistance exercise training where protein synthesis occurs in muscle cells. A greater output of nitrogen relative to its intake indicates protein use for energy and a possible enc ...
... tissue is being synthesized. This is often observed in children, during pregnancy, in recovery from illness, and during resistance exercise training where protein synthesis occurs in muscle cells. A greater output of nitrogen relative to its intake indicates protein use for energy and a possible enc ...
The SMN Complex Is Associated with snRNPs throughout Their
... the entire process of their biogenesis in the cytoplasm and may have multiple functions throughout this process. The small nuclear ribonucleoprotein particles (snRNPs) consist of snRNAs (U1, U2, U4/U6, or U5), several specific proteins that are unique to each snRNA, and a set of seven common Sm prot ...
... the entire process of their biogenesis in the cytoplasm and may have multiple functions throughout this process. The small nuclear ribonucleoprotein particles (snRNPs) consist of snRNAs (U1, U2, U4/U6, or U5), several specific proteins that are unique to each snRNA, and a set of seven common Sm prot ...
Accumulation of xylem transported protein at pit membranes and
... very similar at 19 52561 382 and 19 14861 744 microplate fluorescence units, respectively (means 6SD, n¼4). Transpiring, leafy tobacco shoots and olive twigs, i.e. explants, were allowed to take up fluorescent albumin solution via their cut bases and confocal laser scanning microscope images of fres ...
... very similar at 19 52561 382 and 19 14861 744 microplate fluorescence units, respectively (means 6SD, n¼4). Transpiring, leafy tobacco shoots and olive twigs, i.e. explants, were allowed to take up fluorescent albumin solution via their cut bases and confocal laser scanning microscope images of fres ...
Refining the Definition of Plant Mitochondrial
... of whole Arabidopsis and rice mitochondria. In conventional protein identifications after trypsin digestion using MS/MS peptide spectra, searches are usually undertaken using parameters that require peptides to be products of a defined protease digestion (e.g. trypsin digestion) of a set of precurso ...
... of whole Arabidopsis and rice mitochondria. In conventional protein identifications after trypsin digestion using MS/MS peptide spectra, searches are usually undertaken using parameters that require peptides to be products of a defined protease digestion (e.g. trypsin digestion) of a set of precurso ...
Promiscuity of the Euonymus Carbohydrate-Binding Domain
... lower plant Physcomitrella patens (PhypaEULS3, JGI: scaffold_74 (415096:416617), and the S2 type EUL protein from the monocot Oryza sativa (Os07g0684000, OrysaEULS2). Analysis of the EUL sequences ArathEULS3, PhypaEULS3 and OrysaEULS2 revealed that the EUL domains from these different plant species ...
... lower plant Physcomitrella patens (PhypaEULS3, JGI: scaffold_74 (415096:416617), and the S2 type EUL protein from the monocot Oryza sativa (Os07g0684000, OrysaEULS2). Analysis of the EUL sequences ArathEULS3, PhypaEULS3 and OrysaEULS2 revealed that the EUL domains from these different plant species ...
Biomarker discovery for psychiatric disorders
... established and optimized. Mouse synaptosome proteome profiling was carried out to create a reference map for quantitative proteomics experiments. Furthermore, the quantitative proteomics platform based on metabolic labeling of the HAB/NAB/LAB mouse model with the isotope was established, and the ...
... established and optimized. Mouse synaptosome proteome profiling was carried out to create a reference map for quantitative proteomics experiments. Furthermore, the quantitative proteomics platform based on metabolic labeling of the HAB/NAB/LAB mouse model with the isotope was established, and the ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.