Biological significance of structural differences between two highly
... suggesting that they recognize the surface region shared by the two proteins. This is not surprising given that the two proteins share >90% sequence identity in the core domain and adapt very similar folding in their secondary structures. As expected, Mabs that are positive for Uev1, but negative fo ...
... suggesting that they recognize the surface region shared by the two proteins. This is not surprising given that the two proteins share >90% sequence identity in the core domain and adapt very similar folding in their secondary structures. As expected, Mabs that are positive for Uev1, but negative fo ...
BVGH - Who We Are
... Recombinant or purified protein vaccines consist of protein antigens that have either been produced in a heterologous expression system (e.g., bacteria or yeast) or purified from large amounts of the pathogenic organism. The vaccinated person produces antibodies to the protein antigen, thus protecti ...
... Recombinant or purified protein vaccines consist of protein antigens that have either been produced in a heterologous expression system (e.g., bacteria or yeast) or purified from large amounts of the pathogenic organism. The vaccinated person produces antibodies to the protein antigen, thus protecti ...
Expression of the LXR Protein in Human Atherosclerotic Lesions
... To clarify the distribution of the LXR␣ protein in human atherosclerotic lesions, we examined the lesioned aorta of human subjects. As can be seen in Figure 6A and 6D, in human plaque lesions the LXR␣ protein was mainly detected in the nucleus of mononuclear cells and foam cells. LXR␣positive cells ...
... To clarify the distribution of the LXR␣ protein in human atherosclerotic lesions, we examined the lesioned aorta of human subjects. As can be seen in Figure 6A and 6D, in human plaque lesions the LXR␣ protein was mainly detected in the nucleus of mononuclear cells and foam cells. LXR␣positive cells ...
2006 Program
... “Production of the cold shock protein CspA by Staphylococcus aureus” Andrei Pomerantsev; Tallant-Blanco C.; Yeh, L; Gomis-Ruth, F.X.; Leppla, S.H. “Cre/Lox application to functional genomics of bacteria” Kimberly J. Mueller, Maurine D. Miner, Dr. Nancy E. Freitag “Novel mutations contributing to the ...
... “Production of the cold shock protein CspA by Staphylococcus aureus” Andrei Pomerantsev; Tallant-Blanco C.; Yeh, L; Gomis-Ruth, F.X.; Leppla, S.H. “Cre/Lox application to functional genomics of bacteria” Kimberly J. Mueller, Maurine D. Miner, Dr. Nancy E. Freitag “Novel mutations contributing to the ...
Soy Allergy Doc - Amherst College
... we purchase many items that contain soybean oil in them. Deep frying oil is made of canola oil Avoid any sauces as there is a great possibility that soybean oil is an ingredient. Ask the Chef or Dining Hall Manager for ingredients if there is a sauce that you would like to try out. For the Stir Fry ...
... we purchase many items that contain soybean oil in them. Deep frying oil is made of canola oil Avoid any sauces as there is a great possibility that soybean oil is an ingredient. Ask the Chef or Dining Hall Manager for ingredients if there is a sauce that you would like to try out. For the Stir Fry ...
THE ISOLATION OF WHEAT GERM NUCLEI AND SOME ASPECTS
... The present report deals with the isolation of wheat germ nuclei and with some aspects of their glycolytic metabolism. It has been the objective of this study to determine whether or not the nucleus has an energy-yielding metabolism of its own. The view here adopted is that a knowledge of the type o ...
... The present report deals with the isolation of wheat germ nuclei and with some aspects of their glycolytic metabolism. It has been the objective of this study to determine whether or not the nucleus has an energy-yielding metabolism of its own. The view here adopted is that a knowledge of the type o ...
EVOLUTIONARILY RELATED INSERTION PATHWAYS OF
... presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blobel (1980), in his signal hypothesis, proposed that membrane proteins contain sig ...
... presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blobel (1980), in his signal hypothesis, proposed that membrane proteins contain sig ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Tumor necrosis factor alpha ...
... Tumor necrosis factor alpha ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... The first work comprises the isolation and cDNA cloning of a new carboxypeptidase inhibitor from ticks, named TCI. The recombinant form of this protein is extensively characterized in terms of stability and function, and its possible biological activity is discussed. This work was done in part in Mu ...
... The first work comprises the isolation and cDNA cloning of a new carboxypeptidase inhibitor from ticks, named TCI. The recombinant form of this protein is extensively characterized in terms of stability and function, and its possible biological activity is discussed. This work was done in part in Mu ...
Lens Major Intrinsic Protein (MIP)
... 2: Aggregation of PC-Mbs with PS vesicles detected by the turbidity assay. The absorbance time course at 350 nm was monitored when PC-Mbs vesicles (-) and PC vesicles (A)were mixed with 25 pM PS vesicles. Vesicles were incubated in the same solution described for Figure 1. After 3 min of preincubati ...
... 2: Aggregation of PC-Mbs with PS vesicles detected by the turbidity assay. The absorbance time course at 350 nm was monitored when PC-Mbs vesicles (-) and PC vesicles (A)were mixed with 25 pM PS vesicles. Vesicles were incubated in the same solution described for Figure 1. After 3 min of preincubati ...
Characterization of the Cytosolic Proteins Involved in the Amoeboid
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
Infant Formula And Medical Foods
... Caloric Density when prepared as directed. Calories (kcal) per fluid ounce (fl oz) unless specified differently. Iron Content per liter when prepared as directed. Milligrams (mg) per liter (L) unless specified differently. Yield in fl oz of one oz can of powder, when mixed to standard dilution. Nutr ...
... Caloric Density when prepared as directed. Calories (kcal) per fluid ounce (fl oz) unless specified differently. Iron Content per liter when prepared as directed. Milligrams (mg) per liter (L) unless specified differently. Yield in fl oz of one oz can of powder, when mixed to standard dilution. Nutr ...
Temperature, pressure, and electrochemical
... bonds between sidechain groups and the alpha carbon of the backbone group; the second bond of the proline sidechain group is to the nitrogen of the backbone group. Note that the formulas given in (c) are those of the corresponding amino acid. ...
... bonds between sidechain groups and the alpha carbon of the backbone group; the second bond of the proline sidechain group is to the nitrogen of the backbone group. Note that the formulas given in (c) are those of the corresponding amino acid. ...
Early events in protein folding
... residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible configurations. If the sampling takes place at a rate equal to that of bond vibrations, i.e. 1013 s–1, then it would take 1027 years for an unfolded polypeptide chain ...
... residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible configurations. If the sampling takes place at a rate equal to that of bond vibrations, i.e. 1013 s–1, then it would take 1027 years for an unfolded polypeptide chain ...
Number and spatial distribution of nuclei in the muscle fibres of
... the injected fluorescent dyes, and the fibre type was determined with the following monoclonal antibodies: type 2a, SC-71; type 1, BS-D5; and type 2b, BF-F3 (gift from Stefano Schiaffino; Schiaffino et al. 1989). In the EDL the surface fibres are generally type 2b, and all of the injected fibres wer ...
... the injected fluorescent dyes, and the fibre type was determined with the following monoclonal antibodies: type 2a, SC-71; type 1, BS-D5; and type 2b, BF-F3 (gift from Stefano Schiaffino; Schiaffino et al. 1989). In the EDL the surface fibres are generally type 2b, and all of the injected fibres wer ...
Correlating ribosome function with high
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
Microtubules
... The minus end of a-tubulin may contribute an essential residue to the catalytic site of b-tubulin. Thus the minus end of an a subunit may serve as GAP (GTPase activating protein) for b-tubulin of the adjacent dimer in a protofilament. A homologous bacterial protein FtsZ is considered the ancestor of ...
... The minus end of a-tubulin may contribute an essential residue to the catalytic site of b-tubulin. Thus the minus end of an a subunit may serve as GAP (GTPase activating protein) for b-tubulin of the adjacent dimer in a protofilament. A homologous bacterial protein FtsZ is considered the ancestor of ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... The enzymes specifically aimed at detoxification of superoxide are SODs. The SODs are widely distributed enzymes, present in all kingdoms of life. The family of enzymes with common name SOD consists of three unrelated enzyme types with the ability to catalyze dismutation of superoxide anion into eit ...
... The enzymes specifically aimed at detoxification of superoxide are SODs. The SODs are widely distributed enzymes, present in all kingdoms of life. The family of enzymes with common name SOD consists of three unrelated enzyme types with the ability to catalyze dismutation of superoxide anion into eit ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.