PCNA Protein Expression during Spermatogenesis of the
... entire open reading frame of the PCNA yielded a single band (data not shown), indicating that only one species of mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amin ...
... entire open reading frame of the PCNA yielded a single band (data not shown), indicating that only one species of mRNA was responsible for the production of the two PCNA proteins. To further characterize these two PCNA proteins, we used antibodies that recognized different epitopes of eel PCNA (amin ...
Chapter 5 The Structure and Function of Large Biological Molecules
... A) bonding together of several polypeptide chains by weak bonds. B) order in which amino acids are joined in a polypeptide chain. C) unique three-dimensional shape of the fully folded polypeptide. D) organization of a polypeptide chain into an α helix or β pleated sheet. E) overall protein structure ...
... A) bonding together of several polypeptide chains by weak bonds. B) order in which amino acids are joined in a polypeptide chain. C) unique three-dimensional shape of the fully folded polypeptide. D) organization of a polypeptide chain into an α helix or β pleated sheet. E) overall protein structure ...
pdf-1 - The Wolfson Centre for Applied Structural Biology
... variety of research and diagnostic applications. No other current technology allows researchers to design and manufacture such highly specific molecular recognition tools. In addition to their high specificity, several important features make antibodies particularly conducive to development as probe ...
... variety of research and diagnostic applications. No other current technology allows researchers to design and manufacture such highly specific molecular recognition tools. In addition to their high specificity, several important features make antibodies particularly conducive to development as probe ...
Lewis lung carcinoma regulation of mechanical stretch - AJP-Cell
... newly synthesized protein was determined by Western blots. Myotube diameter measurement. C2C12 myotube diameter was quantified as previously published (69). Briefly, the diameter of each ...
... newly synthesized protein was determined by Western blots. Myotube diameter measurement. C2C12 myotube diameter was quantified as previously published (69). Briefly, the diameter of each ...
Detergents and Surfactants
... charges from residual anionic (negative ions) detergent molecules. Since the negative charges repel each other, the positive cationic detergent neutralizes this charge. It may be surprising that it even works because the ammonium (+1) nitrogen is buried under the methyl groups as can be seen in the ...
... charges from residual anionic (negative ions) detergent molecules. Since the negative charges repel each other, the positive cationic detergent neutralizes this charge. It may be surprising that it even works because the ammonium (+1) nitrogen is buried under the methyl groups as can be seen in the ...
Structure, mechanism and function of prenyltransferases
... has essential biological functions (Table 1). A general mechanism of product chain-length determination and the reaction kinetics derived from a pre-steady-state kinetic analysis for trans-IPPS and cis-IPPS are described. ...
... has essential biological functions (Table 1). A general mechanism of product chain-length determination and the reaction kinetics derived from a pre-steady-state kinetic analysis for trans-IPPS and cis-IPPS are described. ...
PDF - Walter Lab
... (Figure 2, lanes 4 and 5). In particular, three additional bands of higher molecular weight were visible that correspond to the addition of one, two, or three core-oligosaccharide moieties, and are labeled paF.1, paF.2, and paF.3. Increasing the membrane concentration caused a shift toward the fully ...
... (Figure 2, lanes 4 and 5). In particular, three additional bands of higher molecular weight were visible that correspond to the addition of one, two, or three core-oligosaccharide moieties, and are labeled paF.1, paF.2, and paF.3. Increasing the membrane concentration caused a shift toward the fully ...
PDF - ScienceAsia
... set at 200 °C. Helium was used as the carrier gas. The oven temperature protocol was to hold at 65 °C for 5 min, increase 5 °C/min for 16 min, hold for 3 min, then increase again for another 3 min, and finally hold at 200 °C for 5 min. The standardization was done under the same conditions, except t ...
... set at 200 °C. Helium was used as the carrier gas. The oven temperature protocol was to hold at 65 °C for 5 min, increase 5 °C/min for 16 min, hold for 3 min, then increase again for another 3 min, and finally hold at 200 °C for 5 min. The standardization was done under the same conditions, except t ...
Zaenab Aljassim thesis-6_1
... or, in their absence, by the Head of the Department in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that this copy is being made avai ...
... or, in their absence, by the Head of the Department in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that this copy is being made avai ...
Cloning vectors for the expression of green fluorescent protein
... A series of versatile cloning vectors has been constructed that facilitate the expression of protein fusions to the Aequorea victoria green fluorescent protein (GFP) in plant cells. Amino-terminal- and carboxy-terminal protein fusions have been created and visualized by epifluorescence microscopy, b ...
... A series of versatile cloning vectors has been constructed that facilitate the expression of protein fusions to the Aequorea victoria green fluorescent protein (GFP) in plant cells. Amino-terminal- and carboxy-terminal protein fusions have been created and visualized by epifluorescence microscopy, b ...
Enzymatic activation of sulfur for incorporation into biomolecules in
... 6 mM–5.2 mM (http://www.brenda.uni-koeln.de) but many reports give Km-values of around 0.5 mM. Direct estimates of cellular sulfide concentrations have only rarely been reported and fall in the range 20–160 mM (Schmidt & Jäger, 1992; Wang, 2002; Theissen et al., 2003). It is interesting to note tha ...
... 6 mM–5.2 mM (http://www.brenda.uni-koeln.de) but many reports give Km-values of around 0.5 mM. Direct estimates of cellular sulfide concentrations have only rarely been reported and fall in the range 20–160 mM (Schmidt & Jäger, 1992; Wang, 2002; Theissen et al., 2003). It is interesting to note tha ...
Comparison of the activities of protein disulphide
... the assay was then converted into the quantity of RNAase in the PDI incubation by a factor F, which depended on the volumes used. When the assay sample (A ,ll) was withdrawn from the PDI incubation (original volume V,ul) and assayed in the cuvette (C ,tl), F = CV/A. Thus I ,umol of RNAase generated ...
... the assay was then converted into the quantity of RNAase in the PDI incubation by a factor F, which depended on the volumes used. When the assay sample (A ,ll) was withdrawn from the PDI incubation (original volume V,ul) and assayed in the cuvette (C ,tl), F = CV/A. Thus I ,umol of RNAase generated ...
Engineering subunit association of multisubunit proteins
... analysis of complexes, assumes, as a first-order approximation, rigid body association. The same binding free energy evaluation model could also be used to address the third problem for further design of dimeric streptavidins, in which the biotinbinding affinity, reduced due to the subunit separatio ...
... analysis of complexes, assumes, as a first-order approximation, rigid body association. The same binding free energy evaluation model could also be used to address the third problem for further design of dimeric streptavidins, in which the biotinbinding affinity, reduced due to the subunit separatio ...
Chapter 4 Calsequestrin - Department of Molecular Physiology and
... pH (R. A. F. Reithmeier, 1983). This result suggests that the mobility of calsequestrin is sensitive to pH rather than to differences in the buffer system employed. Michalak et al. (1980) showed that calsequestrin could be identified in a mixture of cellular proteins if they were separated first at ...
... pH (R. A. F. Reithmeier, 1983). This result suggests that the mobility of calsequestrin is sensitive to pH rather than to differences in the buffer system employed. Michalak et al. (1980) showed that calsequestrin could be identified in a mixture of cellular proteins if they were separated first at ...
88KB
... nucleotide analogue interference mapping.21 Using these techniques, bases with unusually shifted pKa values have been observed adjacent to the active sites of the lead-dependent ribozyme22 and the hairpin ribozyme,23 and in the peptidyl transferase center of the ribosome.20,24 To measure the pKa of ...
... nucleotide analogue interference mapping.21 Using these techniques, bases with unusually shifted pKa values have been observed adjacent to the active sites of the lead-dependent ribozyme22 and the hairpin ribozyme,23 and in the peptidyl transferase center of the ribosome.20,24 To measure the pKa of ...
G a - Pontificia Universidad Javeriana, Cali
... Using a Timed Concurrent Constraint Process Calculus for Modeling Biomolecular Interactions ...
... Using a Timed Concurrent Constraint Process Calculus for Modeling Biomolecular Interactions ...
The Plant Cell - Semantic Scholar
... The phylogenetic study in Figure 1 showed that FtsH1, FtsH6, and FtsH8 are related closely to VAR1/VAR2. This finding prompted us to examine whether a mutation at either locus would show any phenotype similar to that of var mutants. To study this possibility, we obtained transgenic lines in which T- ...
... The phylogenetic study in Figure 1 showed that FtsH1, FtsH6, and FtsH8 are related closely to VAR1/VAR2. This finding prompted us to examine whether a mutation at either locus would show any phenotype similar to that of var mutants. To study this possibility, we obtained transgenic lines in which T- ...
Chemistry 433 BIOCHEMISTRY LABORATORY MANUAL
... methods portion, unless the methods involved are novel or are crucial to understanding the findings presented. Thousands of papers are published every week. Most literature database search engines include the title and abstract, but do not include the remainder of the paper. In writing the abstract, ...
... methods portion, unless the methods involved are novel or are crucial to understanding the findings presented. Thousands of papers are published every week. Most literature database search engines include the title and abstract, but do not include the remainder of the paper. In writing the abstract, ...
Rodolfo GhirlandoƗ, Radina Mutskova¥, Chad
... PARTIAL SPECIFIC VOLUME AND MOLAR MASS DETERMINATION To obtain an estimate of the molar mass, we assumed that this species had the same size and shape as apoferritin and fixed f/fo to 1.27; the value for the partial specific volume was refined in the data analysis returning a molar mass of 890 kDa. ...
... PARTIAL SPECIFIC VOLUME AND MOLAR MASS DETERMINATION To obtain an estimate of the molar mass, we assumed that this species had the same size and shape as apoferritin and fixed f/fo to 1.27; the value for the partial specific volume was refined in the data analysis returning a molar mass of 890 kDa. ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.