Titration curves of proteins
... we get again the Poisson equation. The advantage of the LPBE is the additivity of electrostatic potentials and charge densities, what makes calculations faster and easier. A more detailed discussion about the PBE and the Debye-Hückel theory is given elsewhere, as for instance in chapter 15-1 (pp. 32 ...
... we get again the Poisson equation. The advantage of the LPBE is the additivity of electrostatic potentials and charge densities, what makes calculations faster and easier. A more detailed discussion about the PBE and the Debye-Hückel theory is given elsewhere, as for instance in chapter 15-1 (pp. 32 ...
Substrate Specificity and Mechanism from the Structure of
... in an anti conformation with the adenine ring situated in a hydrophobic pocket formed by the side-chains of residues Phe52, Trp69, Ile94, Phe110 and Leu100 (Figure 4(a)). This anti conformation of the adenine ring contrasts with the syn conformation proposed in the recent structure determination of ...
... in an anti conformation with the adenine ring situated in a hydrophobic pocket formed by the side-chains of residues Phe52, Trp69, Ile94, Phe110 and Leu100 (Figure 4(a)). This anti conformation of the adenine ring contrasts with the syn conformation proposed in the recent structure determination of ...
Utilisation of Whey
... glycomacropeptide (GMP) because it usually contains a number of carbohydrate attachments (hence the "glyco-" term). Its molecular weight, based on its amino acid content, is about 7,000; however, the carbohydrate units make it behave as if it were somewhat larger. This is important because the ultra ...
... glycomacropeptide (GMP) because it usually contains a number of carbohydrate attachments (hence the "glyco-" term). Its molecular weight, based on its amino acid content, is about 7,000; however, the carbohydrate units make it behave as if it were somewhat larger. This is important because the ultra ...
MODified™ Protein Domain Binding Kit Manual
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
Protein structure is conceptually divided into four
... of the connections of four antiparallel b strands is not a hairpin connection. The motif occurs when strand number n (in the barrel, NOT in the sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different pos ...
... of the connections of four antiparallel b strands is not a hairpin connection. The motif occurs when strand number n (in the barrel, NOT in the sequence) is connected to strand n + 3 (a) or n - 3 (b) instead of n + 1 or n - 1 in an eight-stranded antiparallel b sheet or barrel. The two different pos ...
general-organic-and-biological-chemistry-3rd-edition
... 21. The periodic table of the elements does not list whole numbers for atomic weights. Why? A. The atomic weights are not predictable. B. The atomic weights include protons and neutrons at 1 amu each, but they also include electrons, which weigh a lot less than one. C. The atomic weight is the weigh ...
... 21. The periodic table of the elements does not list whole numbers for atomic weights. Why? A. The atomic weights are not predictable. B. The atomic weights include protons and neutrons at 1 amu each, but they also include electrons, which weigh a lot less than one. C. The atomic weight is the weigh ...
Similarities and Differences in the Glycosylation Mechanisms in
... Recent years have witnessed a rapid growth in the number and diversity of prokaryotic proteins shown to carry N- and/or Oglycans, with protein glycosylation now considered as fundamental to the biology of these organisms as it is in eukaryotic systems. This article overviews the major glycosylation ...
... Recent years have witnessed a rapid growth in the number and diversity of prokaryotic proteins shown to carry N- and/or Oglycans, with protein glycosylation now considered as fundamental to the biology of these organisms as it is in eukaryotic systems. This article overviews the major glycosylation ...
The paradox of elongation factor 4: highly conserved, yet of no
... the PRE complex, competing with EF-G to inhibit the elongation cycle [79,80] (Figure 1). The rate of the reaction of EF4 with the PRE complex is as rapid as that of EF-G with the PRE complex [80]. Such effects of EF4 would be expected to slow down peptide synthesis and thereby facilitate co-translat ...
... the PRE complex, competing with EF-G to inhibit the elongation cycle [79,80] (Figure 1). The rate of the reaction of EF4 with the PRE complex is as rapid as that of EF-G with the PRE complex [80]. Such effects of EF4 would be expected to slow down peptide synthesis and thereby facilitate co-translat ...
Lecture#5 File
... 550 amino acids arranged in two chains HA1 and HA2. The first half of each chain has a lighter color in the diagram. A long stem like region, built up by residues from both chains, includes one of the longest a helices known in a globular structure, about 75 Å long. The globular head is formed by re ...
... 550 amino acids arranged in two chains HA1 and HA2. The first half of each chain has a lighter color in the diagram. A long stem like region, built up by residues from both chains, includes one of the longest a helices known in a globular structure, about 75 Å long. The globular head is formed by re ...
Gene Section FA1 (Fanconi anaemia 1) Atlas of Genetics and Cytogenetics
... Lo Ten Foe JR, Rooimans MA, Bosnoyan-Collins L, Alon N, Wijker M, Parker L, Lightfoot J, Carreau M, Callen DF, Savoia A, Cheng NC, van Berkel CG, Strunk MH, Gille JJ, Pals G, Kruyt FA, Pronk JC, Arwert F, Buchwald M, Joenje H. Expression cloning of a cDNA for the major Fanconi anaemia gene, FAA. Nat ...
... Lo Ten Foe JR, Rooimans MA, Bosnoyan-Collins L, Alon N, Wijker M, Parker L, Lightfoot J, Carreau M, Callen DF, Savoia A, Cheng NC, van Berkel CG, Strunk MH, Gille JJ, Pals G, Kruyt FA, Pronk JC, Arwert F, Buchwald M, Joenje H. Expression cloning of a cDNA for the major Fanconi anaemia gene, FAA. Nat ...
Structure and function of the eukaryotic ADP
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
Chapter 5 Photosynthesis
... A proton transfer across the membrane is coupled to the conformational transition. Halobacteria are, however, not able to use carbon dioxide as sole carbon source. Since the photosynthetic mechanism of these bacteria is fundamentally different to the oxygenic photosynthesis or anoxygenic photosynthe ...
... A proton transfer across the membrane is coupled to the conformational transition. Halobacteria are, however, not able to use carbon dioxide as sole carbon source. Since the photosynthetic mechanism of these bacteria is fundamentally different to the oxygenic photosynthesis or anoxygenic photosynthe ...
A fluorophore ligase for site-specific protein labeling inside living cells
... could then be chemoselectively derivatized using cyclooctynefluorophore conjugates. In this work, we wished to extend LplA-mediated labeling to intracellular proteins but recognized the challenges associated with our two-step labeling scheme. First, labeling sensitivity is limited by the kinetics of ...
... could then be chemoselectively derivatized using cyclooctynefluorophore conjugates. In this work, we wished to extend LplA-mediated labeling to intracellular proteins but recognized the challenges associated with our two-step labeling scheme. First, labeling sensitivity is limited by the kinetics of ...
Characterization and the role of carbonic anhydrase
... Characterization and the role of carbonic anhydrase activity in Microalgae 2.2. Kinetic study on carbonic anhydrase assay activity (Armstrong et al., 1996) Carbonic anhydrase activity was determined in 50 μl of extracted carboxysomal protein from Synechococcus sp. and Fischerella muscicola. Tris su ...
... Characterization and the role of carbonic anhydrase activity in Microalgae 2.2. Kinetic study on carbonic anhydrase assay activity (Armstrong et al., 1996) Carbonic anhydrase activity was determined in 50 μl of extracted carboxysomal protein from Synechococcus sp. and Fischerella muscicola. Tris su ...
Basic region of residues 228-231 of protein kinase CK1[alpha] is
... in these assays was produced by 35S labeling through an in vitro transcription-translation system or by allowing CK1a to autophosphorylate with 32P. Alternatively, the presence of active CK1a bound to axin on the sepharose beads can be assayed by determining its capacity to phosphorylate a specific ...
... in these assays was produced by 35S labeling through an in vitro transcription-translation system or by allowing CK1a to autophosphorylate with 32P. Alternatively, the presence of active CK1a bound to axin on the sepharose beads can be assayed by determining its capacity to phosphorylate a specific ...
BEL β-trefoil: A novel lectin with antineoplastic properties in king
... and the second used a column of human erythrocytic stroma incorporated into a polyacrylamide gel (Betail et al. 1975). While using the second method it was found that a second lectin was present in very significant quantities in the mushroom extracts. Here, we present our results on this second, tota ...
... and the second used a column of human erythrocytic stroma incorporated into a polyacrylamide gel (Betail et al. 1975). While using the second method it was found that a second lectin was present in very significant quantities in the mushroom extracts. Here, we present our results on this second, tota ...
Gene Section HDAC3 (histone deacetylase 3) Atlas of Genetics and Cytogenetics
... observed in the region 180-313 and these residues act as a NES (or as a binding site for a NES-containing protein) that uses CRM1 export pathway. A NLS has been characterised in the C-terminal region (313-428). Another important sequence, required for oligomerisation of HDAC3 with itself and for the ...
... observed in the region 180-313 and these residues act as a NES (or as a binding site for a NES-containing protein) that uses CRM1 export pathway. A NLS has been characterised in the C-terminal region (313-428). Another important sequence, required for oligomerisation of HDAC3 with itself and for the ...
4 - EMD Millipore
... antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. You will find this guide to be a useful resource, whether you are just beginning your research or you are training the new researchers in your laboratory. Specific techni ...
... antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. You will find this guide to be a useful resource, whether you are just beginning your research or you are training the new researchers in your laboratory. Specific techni ...
Towards the construction of Escherichia coli cell
... In this work, a DNA template was design and purified. The DNA template was purified using an alkaline lysis and hydrophobic interaction chromatography. This method allows us to produce in higher quantity and lower cost. The tRNA purification was achieved by extracting the nucleic acids by phenol ext ...
... In this work, a DNA template was design and purified. The DNA template was purified using an alkaline lysis and hydrophobic interaction chromatography. This method allows us to produce in higher quantity and lower cost. The tRNA purification was achieved by extracting the nucleic acids by phenol ext ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.