Protein Sorting and Transport - The Endoplasmic Reticulum, Golgi
... These proteins have a normal amino-terminal signal sequence, which is cleaved by signal peptidase during translocation of the polypeptide chain across the ER membrane through the Sec61 channel. They are then anchored in the membrane by a second membrane-spanning helix in the middle of the protein. ...
... These proteins have a normal amino-terminal signal sequence, which is cleaved by signal peptidase during translocation of the polypeptide chain across the ER membrane through the Sec61 channel. They are then anchored in the membrane by a second membrane-spanning helix in the middle of the protein. ...
Characterization of the RNase A active site by Phage Panning
... purification were amplified and used to prepare DNA samples for sequence analysis. We analyzed the predicted amino acid sequences of active molecules for common features in order to develop hypotheses about how these proteins may interact with their target. We used Autodock molecular docking softwar ...
... purification were amplified and used to prepare DNA samples for sequence analysis. We analyzed the predicted amino acid sequences of active molecules for common features in order to develop hypotheses about how these proteins may interact with their target. We used Autodock molecular docking softwar ...
(A6103) - Datasheet - Sigma
... agents like 2-mercaptoethanol.5 Due to its compact tertiary structure, aprotinin is relatively stable against denaturation due to high temperature, organic solvents, or proteolytic degradation (See Table 2). Only thermolysin has been found capable of degrading ...
... agents like 2-mercaptoethanol.5 Due to its compact tertiary structure, aprotinin is relatively stable against denaturation due to high temperature, organic solvents, or proteolytic degradation (See Table 2). Only thermolysin has been found capable of degrading ...
PowerPoint Template
... linear, unequivocal flow of information takes place. This is however a simplistic view of the situation in the cell and is mostly born of an incomplete knowledge of the pathway. Branching of Signaling Pathways As more information on the various signaling pathways of the cell was obtained the more ...
... linear, unequivocal flow of information takes place. This is however a simplistic view of the situation in the cell and is mostly born of an incomplete knowledge of the pathway. Branching of Signaling Pathways As more information on the various signaling pathways of the cell was obtained the more ...
Sample Chapter 5: Amino Acids, Peptides, and Proteins
... with more than 50 amino acids. Each protein consists of one or more polypeptide chains. The distinction between proteins and peptides is often imprecise. For example, some biochemists define oligopeptides as polymers consisting of two to ten amino acids and polypeptides as having more than ten resid ...
... with more than 50 amino acids. Each protein consists of one or more polypeptide chains. The distinction between proteins and peptides is often imprecise. For example, some biochemists define oligopeptides as polymers consisting of two to ten amino acids and polypeptides as having more than ten resid ...
pH scale. Buffer solutions. Colligative properties of solutions
... Test papers of universal indicator are used only for an approximate pH of the solution. For precise determination of the pH value, colorimetric or potentiometric methods are used. ...
... Test papers of universal indicator are used only for an approximate pH of the solution. For precise determination of the pH value, colorimetric or potentiometric methods are used. ...
Peptides and proteins Chapter 36:
... by cells of the hypothalamus, some neurons, and by keratinocytes and melanocytes of the skin and scalp. However, this protein does not seem to have a function of its own. Depending on the cell in which it is produced, it is broken down by endopeptidase enzymes into smaller fragments, or peptides, wh ...
... by cells of the hypothalamus, some neurons, and by keratinocytes and melanocytes of the skin and scalp. However, this protein does not seem to have a function of its own. Depending on the cell in which it is produced, it is broken down by endopeptidase enzymes into smaller fragments, or peptides, wh ...
First Poly(2-oxazoline)s with Pendant Amino Groups
... terminal amine groups have been described so far.[16] Our interest in the reactivity of this group is focused on the possibility of synthesizing polymer-peptide conjugates. Through the linking groups introduced in the side chain or as end functionalization, the polymers can be coupled to peptides by ...
... terminal amine groups have been described so far.[16] Our interest in the reactivity of this group is focused on the possibility of synthesizing polymer-peptide conjugates. Through the linking groups introduced in the side chain or as end functionalization, the polymers can be coupled to peptides by ...
Design, Synthesis, Biological Activity and Molecular Dynamics
... the negative regulation of the insulin signaling pathway and a valid potential drug target for the treatment of type 2 diabetes and other associated metabolic syndromes [5,6]. It acts by dephosphorylation of specific phosphotyrosine (pTyr) residues on the insulin receptor and insulin receptor substr ...
... the negative regulation of the insulin signaling pathway and a valid potential drug target for the treatment of type 2 diabetes and other associated metabolic syndromes [5,6]. It acts by dephosphorylation of specific phosphotyrosine (pTyr) residues on the insulin receptor and insulin receptor substr ...
Journal of Agricultural and Food Chemistry
... 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stability and structure of cereal-like plant proteins, such as amaranth and quinoa, which can be used as dietary proteins in food systems. The present study focuses on the denaturant-induced secondary and tertiary str ...
... 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stability and structure of cereal-like plant proteins, such as amaranth and quinoa, which can be used as dietary proteins in food systems. The present study focuses on the denaturant-induced secondary and tertiary str ...
Mitochondrial protein acetylation regulates metabolism
... example, mitochondrial protein acetylation increases in the liver during fasting. In mice, 62% of acetylated mitochondrial proteins were identified in mitochondrial fractions isolated from both fed and fasted animals, whereas 14% of acetylated mitochondrial proteins were unique to fed mice, and 24% ...
... example, mitochondrial protein acetylation increases in the liver during fasting. In mice, 62% of acetylated mitochondrial proteins were identified in mitochondrial fractions isolated from both fed and fasted animals, whereas 14% of acetylated mitochondrial proteins were unique to fed mice, and 24% ...
Clathrinmediated transport: assembly required
... binding sites for phosphatidylinositol (4,5)-bisphosphate (PIP2) have been identified in both the α- and the μ2-subunits and seem to be involved in the recruitment of AP2 to the plasma membrane. Owen presented the crystal structure of a complex comprising the N-terminal two-thirds of the α- and β2-t ...
... binding sites for phosphatidylinositol (4,5)-bisphosphate (PIP2) have been identified in both the α- and the μ2-subunits and seem to be involved in the recruitment of AP2 to the plasma membrane. Owen presented the crystal structure of a complex comprising the N-terminal two-thirds of the α- and β2-t ...
Single-stranded DNA-binding Proteins
... repair, RecA must bind in a generally nonsequence-specific manner to the damaged ssDNA, then bind a second strand (the strand with the homologous but undamaged segment), and the complementary strand that will be required to produce the repaired dsDNA (Figure 3). Since recombination requires that rela ...
... repair, RecA must bind in a generally nonsequence-specific manner to the damaged ssDNA, then bind a second strand (the strand with the homologous but undamaged segment), and the complementary strand that will be required to produce the repaired dsDNA (Figure 3). Since recombination requires that rela ...
Equilibrium in CHNOSZ
... help page (type ?mosaic at the R command line). Step-by-step examples of some of the calculations, particularly the reaction matrix algorithm, are provided in the Appendix. For further description of the equilibration method applied to proteins see Dick and Shock (2013) (also with a derivation of en ...
... help page (type ?mosaic at the R command line). Step-by-step examples of some of the calculations, particularly the reaction matrix algorithm, are provided in the Appendix. For further description of the equilibration method applied to proteins see Dick and Shock (2013) (also with a derivation of en ...
Slides
... Increases the rate of chemical reaction / biological process Remains unchanged Biomolecules that catalyze chemical reactions Usually proteins ...
... Increases the rate of chemical reaction / biological process Remains unchanged Biomolecules that catalyze chemical reactions Usually proteins ...
311-320 - CiteSeerX
... 1960); quenching of the fluorescence is due to the exposure of the buried tryptophan residues. Thus the first event in the denaturation reaction appears to be perturbation of the aromatic amino acid residues. This is followed by the exposure of the buried aromatic amino acids and finally by a confor ...
... 1960); quenching of the fluorescence is due to the exposure of the buried tryptophan residues. Thus the first event in the denaturation reaction appears to be perturbation of the aromatic amino acid residues. This is followed by the exposure of the buried aromatic amino acids and finally by a confor ...
PDF - Biotechnology for Biofuels
... different pH conditions studied. The relative expression levels were presented as a fold change (log2) between the control sample (pH 7.3) and the test sample (pH 6.3). In total more than 290 genes were found to be differentially expressed, with 72% being up-regulated and only 28% down-regulated at ...
... different pH conditions studied. The relative expression levels were presented as a fold change (log2) between the control sample (pH 7.3) and the test sample (pH 6.3). In total more than 290 genes were found to be differentially expressed, with 72% being up-regulated and only 28% down-regulated at ...
Structural biology and drug design
... than one of the following criteria 1. Not more than 5 hydrogen bond donors 2. Not more than 10 hydrogen bond acceptors 3. A molecular mass below 500 Dalton (g/mol) 4. An octanol-water partition-coefficient logP not greater than 5 • These rules ensures that the compound is not too soluble and polar, ...
... than one of the following criteria 1. Not more than 5 hydrogen bond donors 2. Not more than 10 hydrogen bond acceptors 3. A molecular mass below 500 Dalton (g/mol) 4. An octanol-water partition-coefficient logP not greater than 5 • These rules ensures that the compound is not too soluble and polar, ...
3 | biological macromolecules
... Benefits of Carbohydrates Are carbohydrates good for you? People who wish to lose weight are often told that carbohydrates are bad for them and should be avoided. Some diets completely forbid carbohydrate consumption, claiming that a low-carbohydrate diet helps people to lose weight faster. However, ...
... Benefits of Carbohydrates Are carbohydrates good for you? People who wish to lose weight are often told that carbohydrates are bad for them and should be avoided. Some diets completely forbid carbohydrate consumption, claiming that a low-carbohydrate diet helps people to lose weight faster. However, ...
Chemical Reactions - thsicp-23
... Products in combustion are ALWAYS carbon dioxide and water. (although incomplete burning does cause some byproducts like carbon monoxide) Combustion is used to heat homes and run automobiles (octane, as in gasoline, is C8H18) ...
... Products in combustion are ALWAYS carbon dioxide and water. (although incomplete burning does cause some byproducts like carbon monoxide) Combustion is used to heat homes and run automobiles (octane, as in gasoline, is C8H18) ...
Protein Synthesis and the Stress Response
... observation, it has been proposed that the translation activity could be rapidly recovered after oxidative stress through the reduction of cysteines 105 and 242 by thioredoxin. This last protein in turn would be reduced by NADPH-thioredoxin reductase and ferredoxinthioredoxin reductase with reducing ...
... observation, it has been proposed that the translation activity could be rapidly recovered after oxidative stress through the reduction of cysteines 105 and 242 by thioredoxin. This last protein in turn would be reduced by NADPH-thioredoxin reductase and ferredoxinthioredoxin reductase with reducing ...
AlgPred: prediction of allergenic proteins and mapping of
... raghava/algpred/). AlgPred is available at www. imtech.res.in/raghava/algpred/. INTRODUCTION Allergy involves a series of complex reactions and both intrinsic and extrinsic factors contribute to the development of the disease and triggering of the symptoms. Type I hypersensitive reaction is induced ...
... raghava/algpred/). AlgPred is available at www. imtech.res.in/raghava/algpred/. INTRODUCTION Allergy involves a series of complex reactions and both intrinsic and extrinsic factors contribute to the development of the disease and triggering of the symptoms. Type I hypersensitive reaction is induced ...
FES Active (F4930) - Datasheet - Sigma
... baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM 002005. It is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ∼125 kDa Purity: ≥70% (SDS-PAGE, see Figure 1) Specif ...
... baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM 002005. It is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ∼125 kDa Purity: ≥70% (SDS-PAGE, see Figure 1) Specif ...
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
The protein acetylome and the regulation of metabolism - Serval
... (NatC) contains the Maintenance of Killer proteins Mak3, Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amin ...
... (NatC) contains the Maintenance of Killer proteins Mak3, Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amin ...