EVANS GROUP RESEARCH PROJECT DESCRIPTIONS
... heavily investigated in the past, they offer new options for polymer synthesis. Organolanthanide complexes have the added advantage that they can initiate polymerization without an aluminum co-catalyst such as MAO. Efforts to define the basic mechanisms of lanthanide-based olefin polymerization with ...
... heavily investigated in the past, they offer new options for polymer synthesis. Organolanthanide complexes have the added advantage that they can initiate polymerization without an aluminum co-catalyst such as MAO. Efforts to define the basic mechanisms of lanthanide-based olefin polymerization with ...
Unit IX: Identification of a Gram
... For each unknown and media, inoculate three slants using three different colonies on the selective media that look like either salmonella or shigella, or like a coliform. The reason for using three slants is that you may have difficulty distinguishing Proteus from the SS pathogens. By inoculating th ...
... For each unknown and media, inoculate three slants using three different colonies on the selective media that look like either salmonella or shigella, or like a coliform. The reason for using three slants is that you may have difficulty distinguishing Proteus from the SS pathogens. By inoculating th ...
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular
... Particle pairs may be selected by use of the mouse over the matrix. Hovering performs a preselection and clicking a selection. Individual particles are preselected and selected when the mouse is just below the main diagonal of the matrix. Selections and preselections are kept consistent between the ...
... Particle pairs may be selected by use of the mouse over the matrix. Hovering performs a preselection and clicking a selection. Individual particles are preselected and selected when the mouse is just below the main diagonal of the matrix. Selections and preselections are kept consistent between the ...
Thermo Scientific Solutions for Quantitative
... from LC-MS/MS runs and integrating peak areas over the chromatographic time scale. Typically favored for shotgun proteomics, the label-free approach has been incorporated into large-scale biomarker discovery studies measuring disease-related changes. It has demonstrated high reproducibility and line ...
... from LC-MS/MS runs and integrating peak areas over the chromatographic time scale. Typically favored for shotgun proteomics, the label-free approach has been incorporated into large-scale biomarker discovery studies measuring disease-related changes. It has demonstrated high reproducibility and line ...
Theranostics Evolution- and Structure
... Although most variations in protein sequence are predicted to have little or no effect on protein function, some nsSNPs are known to be associated with disease. These disease-associated nsSNPs have diverse effects on protein properties and may affect a protein’s stability, catalytic activity, and/or ...
... Although most variations in protein sequence are predicted to have little or no effect on protein function, some nsSNPs are known to be associated with disease. These disease-associated nsSNPs have diverse effects on protein properties and may affect a protein’s stability, catalytic activity, and/or ...
On The Determination of Enzyme Structure, Function, and
... their ability to interact with X-rays. The experimental setup (see Figure 3) can be described as follows: a narrow beam of monochromatic X-rays of suitable wavelength is directed to the crystal; X-rays, being electromagnetic waves, either traverse straight through the crystal, in between the enzyme ...
... their ability to interact with X-rays. The experimental setup (see Figure 3) can be described as follows: a narrow beam of monochromatic X-rays of suitable wavelength is directed to the crystal; X-rays, being electromagnetic waves, either traverse straight through the crystal, in between the enzyme ...
Flavors of Protein Disorder
... than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D structure is likely encoded by the amino acid sequence11 and, furthermore, regions lacking specific 3 ...
... than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D structure is likely encoded by the amino acid sequence11 and, furthermore, regions lacking specific 3 ...
UltraClean 15 DNA Purification Kit
... We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be used. It is not necessary to use low melt agarose for good recoveries with this kit. If the ULTRA BIND Dries Out It ...
... We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be used. It is not necessary to use low melt agarose for good recoveries with this kit. If the ULTRA BIND Dries Out It ...
Kodaq 2X PCR MasterMix
... reaction buffer and a sophisticated blend of additives in a 2X concentration). The Kodaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. Kodaq 2X PCR MasterMix is the most robust PCR MasterMix ...
... reaction buffer and a sophisticated blend of additives in a 2X concentration). The Kodaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. Kodaq 2X PCR MasterMix is the most robust PCR MasterMix ...
Iron Oxyhydroxide Aerogels and Xerogels by Hydrolysis of FeCl3 ∙ 6
... only exception is the final step, which is more simple, namely ethanol can be used to remove the organic solvents instead of extraction with supercritical CO2. In consequence, the obtained oxyhydroxide gel is more compact, although the first stages of formation, the process of hydrolysis are the sam ...
... only exception is the final step, which is more simple, namely ethanol can be used to remove the organic solvents instead of extraction with supercritical CO2. In consequence, the obtained oxyhydroxide gel is more compact, although the first stages of formation, the process of hydrolysis are the sam ...
Database searching
... Building a Profile HMM Multiple alignment is used to construct the HMM model. Assign each column to a Match state in HMM. Add Insertion and Deletion state. Estimate the emission probabilities according to amino acid counts in column. Different positions in the protein will have different emis ...
... Building a Profile HMM Multiple alignment is used to construct the HMM model. Assign each column to a Match state in HMM. Add Insertion and Deletion state. Estimate the emission probabilities according to amino acid counts in column. Different positions in the protein will have different emis ...
ANALYSIS OF THE SILVER GROUP CATIONS
... be useful later. For example, AgCl acquires a slight purplish tint on standing in light for a while. Also, the crystals of PbCl2 look more like snow than the denser solid, AgCl. 2. In separating these ions from others, it is important to add enough HCl to get complete precipitation. Cold HCl is used ...
... be useful later. For example, AgCl acquires a slight purplish tint on standing in light for a while. Also, the crystals of PbCl2 look more like snow than the denser solid, AgCl. 2. In separating these ions from others, it is important to add enough HCl to get complete precipitation. Cold HCl is used ...
Marine Biotechnology
... This is interesting from a physiologic viewpoint because a third function is accomplished by combining 2 enzymes. Because lobsters feed on animal food, their digestive systems have to process connective tissue containing huge amounts of collagen. Lobsters have a protease of low molecular weight, aro ...
... This is interesting from a physiologic viewpoint because a third function is accomplished by combining 2 enzymes. Because lobsters feed on animal food, their digestive systems have to process connective tissue containing huge amounts of collagen. Lobsters have a protease of low molecular weight, aro ...
Molecular Characterization of –Lactalbumin Folding Variants That
... washed with 50 mM Tris/HCl with 1 mM EDTA, pH 7.5, and a-lactalbumin was then eluted from the column with 50 mM Tris/HCl with 1 mM CaCl2, pH 7.5, thus yielding the native, Ca21 bound form of a-lactalbumin. The classical molten globule state was obtained by lowering the pH of a solution of native mon ...
... washed with 50 mM Tris/HCl with 1 mM EDTA, pH 7.5, and a-lactalbumin was then eluted from the column with 50 mM Tris/HCl with 1 mM CaCl2, pH 7.5, thus yielding the native, Ca21 bound form of a-lactalbumin. The classical molten globule state was obtained by lowering the pH of a solution of native mon ...
NMR Analysis of Protein
... • Assigned 2D 1H-15N HSQC NMR Spectra overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peak ...
... • Assigned 2D 1H-15N HSQC NMR Spectra overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peak ...
THE THREE-DIMENSIONAL STRUCTURE OF PROTEINS
... Chapter 2, this entropy term is the major thermodynamic driving force for the association of hydrophobic groups in aqueous solution. Hydrophobic amino acid side chains therefore tend to be clustered in a protein’s interior, away from water. Under physiological conditions, the formation of hydrogen b ...
... Chapter 2, this entropy term is the major thermodynamic driving force for the association of hydrophobic groups in aqueous solution. Hydrophobic amino acid side chains therefore tend to be clustered in a protein’s interior, away from water. Under physiological conditions, the formation of hydrogen b ...
PSLDoc: Protein subcellular localization prediction based on
... the training data set whether it is below or above a similarity threshold of 30%. This classification of data is motivated by an observation that sequence homology and subcellular localization have strong correlation when the sequence identity is higher than 30%. Hence, they also propose a hybrid me ...
... the training data set whether it is below or above a similarity threshold of 30%. This classification of data is motivated by an observation that sequence homology and subcellular localization have strong correlation when the sequence identity is higher than 30%. Hence, they also propose a hybrid me ...
07_Lecture_Presentation
... Concept 7.2: Membrane structure results in selective permeability A cell must exchange materials with its surroundings, a process controlled by the plasma membrane Plasma membranes are selectively permeable, regulating the cell’s molecular traffic ...
... Concept 7.2: Membrane structure results in selective permeability A cell must exchange materials with its surroundings, a process controlled by the plasma membrane Plasma membranes are selectively permeable, regulating the cell’s molecular traffic ...
Supplementary Methods - Word file (146 KB )
... (New England Biolabs) in 1 ml of Taq Ligase buffer at 60 oC for 2 hours. The ligation products were ethanol-precipitated, resuspended in TE buffer, pH 8.0 and separated using 8% denaturing PAGE (40 cm x 1.5 mm). The correct-length ligation product was excised from the gel and extracted using standar ...
... (New England Biolabs) in 1 ml of Taq Ligase buffer at 60 oC for 2 hours. The ligation products were ethanol-precipitated, resuspended in TE buffer, pH 8.0 and separated using 8% denaturing PAGE (40 cm x 1.5 mm). The correct-length ligation product was excised from the gel and extracted using standar ...
Genomic DNA Extraction From Buccal Epithelial Cells
... number in your notebook. Only you will know this anonymous code. 2. To collect buccal epithelial cells, use a sterile toothpick or sterile pipette tip to gently scrape the inside of both cheeks. Scrape well so that you get lots of cells. However, this procedure should be non-invasive so don’t draw b ...
... number in your notebook. Only you will know this anonymous code. 2. To collect buccal epithelial cells, use a sterile toothpick or sterile pipette tip to gently scrape the inside of both cheeks. Scrape well so that you get lots of cells. However, this procedure should be non-invasive so don’t draw b ...
Translocation Arrest by Reversible Folding of a Precursor Protein
... by differential centrifugation were carried out as described (Pfanner and Neupert, 1985), except that the time for grinding of hyphae with sand was reduced to 30 s. Isolated milochondria were resuspended at a concentration of 1 mg/ml in SEM buffer (0.25 M sucrose, I mM EDTA, 10 mM morpholino propane ...
... by differential centrifugation were carried out as described (Pfanner and Neupert, 1985), except that the time for grinding of hyphae with sand was reduced to 30 s. Isolated milochondria were resuspended at a concentration of 1 mg/ml in SEM buffer (0.25 M sucrose, I mM EDTA, 10 mM morpholino propane ...
Lysines 72, 80 and 213 and aspartic acid 210 of the
... (5 min) in the presence of SDS-PAGE sample buffer. Subsequently, total cellular protein was separated on a 12.5% polyacrylamide-SDS gel and transferred to a nitrocellulose membrane (BA85; Schleicher & Schuell). The membrane was treated with rabbit polyclonal LacR antibodies and then incubated with p ...
... (5 min) in the presence of SDS-PAGE sample buffer. Subsequently, total cellular protein was separated on a 12.5% polyacrylamide-SDS gel and transferred to a nitrocellulose membrane (BA85; Schleicher & Schuell). The membrane was treated with rabbit polyclonal LacR antibodies and then incubated with p ...
MEDICAL BIOCHEMISTRY Problem Unit One 1999/2000 pH and
... aqueous, polar environment (e.g. cytoplasm); and the hydrophobic, or lipophilic, non-aqueous environment (e.g. membranes). Hydrophobic compounds are uncharged, nonpolar species and generally contain largely aliphatic and aromatic organic groups. Hydrophilic compounds are polar and include sugars, sa ...
... aqueous, polar environment (e.g. cytoplasm); and the hydrophobic, or lipophilic, non-aqueous environment (e.g. membranes). Hydrophobic compounds are uncharged, nonpolar species and generally contain largely aliphatic and aromatic organic groups. Hydrophilic compounds are polar and include sugars, sa ...
Characterization of the RNase A active site by Phage Panning
... purification were amplified and used to prepare DNA samples for sequence analysis. We analyzed the predicted amino acid sequences of active molecules for common features in order to develop hypotheses about how these proteins may interact with their target. We used Autodock molecular docking softwar ...
... purification were amplified and used to prepare DNA samples for sequence analysis. We analyzed the predicted amino acid sequences of active molecules for common features in order to develop hypotheses about how these proteins may interact with their target. We used Autodock molecular docking softwar ...