Host cell protein analysis by mass spectrometry and its application
... A biosimilar company needs to justify adequate low levels of HCPs for their own process ...
... A biosimilar company needs to justify adequate low levels of HCPs for their own process ...
Cybertory Manual (WP) - Attotron Biosensor Corporation
... the path of the migrating DNA molecules can retard the progress of larger molecules, thus making it possible to separate a mixture of DNA fragments by size. The most commonly used substances for producing a matrix for electrophoretic separation are agarose (a gelatinous substance purified from the t ...
... the path of the migrating DNA molecules can retard the progress of larger molecules, thus making it possible to separate a mixture of DNA fragments by size. The most commonly used substances for producing a matrix for electrophoretic separation are agarose (a gelatinous substance purified from the t ...
Protein Engineering in the Development of
... causing macroscopic changes in hydrogel structures (15, 16); elastin-like polypeptides undergo a reversible inverse phase transition to form insoluble β-spiral aggregates (17, 18); and controlled degradation of a hydrogel matrix is possible with the incorporation of protease cleavage sequences ...
... causing macroscopic changes in hydrogel structures (15, 16); elastin-like polypeptides undergo a reversible inverse phase transition to form insoluble β-spiral aggregates (17, 18); and controlled degradation of a hydrogel matrix is possible with the incorporation of protease cleavage sequences ...
Lac
... milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found in milk of species which transport high levels of immunoglobulins during c ...
... milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found in milk of species which transport high levels of immunoglobulins during c ...
Chromatography
... solute particles to pass through uninhibited. • Small particles permeate gel and are slowed down. The smaller the particles, the longer it takes for them to get through column. • Separation is according to particle size. ...
... solute particles to pass through uninhibited. • Small particles permeate gel and are slowed down. The smaller the particles, the longer it takes for them to get through column. • Separation is according to particle size. ...
Function of Conserved Tryptophans in the Aspergillus niger
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
immobilised metal affinity chromatography (imac) beads for
... obtained by several techniques such as ion exchange chromatography, ultrafiltration and affinity chromatography. However rigorous limitations occur including lengthy steps, complicated and expensive for large scale production has hampered the applications. Separation technique for protein by Immobil ...
... obtained by several techniques such as ion exchange chromatography, ultrafiltration and affinity chromatography. However rigorous limitations occur including lengthy steps, complicated and expensive for large scale production has hampered the applications. Separation technique for protein by Immobil ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
... the ovule by its polarily growing pollen tube. Yet the molecular genetic basis of these functions is poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, ...
From Structure to Function
... analogy holds for many proteins and ligands: more than one key will sometimes fit into a lock but most other keys will not, and usually only the right key will open the lock. Similarly, more than one ligand will sometimes fit into a binding site on the surface of a protein, but most other ligands wi ...
... analogy holds for many proteins and ligands: more than one key will sometimes fit into a lock but most other keys will not, and usually only the right key will open the lock. Similarly, more than one ligand will sometimes fit into a binding site on the surface of a protein, but most other ligands wi ...
Biochemistry Syllabus
... 2. I can explain how the shape of enzymes, active sites and interaction with specific molecules are essential for basic functioning of the enzyme. a. I can explain how for an enzyme-mediated chemical reaction to occur, the substrate must be complementary to the surface properties (shape and charge o ...
... 2. I can explain how the shape of enzymes, active sites and interaction with specific molecules are essential for basic functioning of the enzyme. a. I can explain how for an enzyme-mediated chemical reaction to occur, the substrate must be complementary to the surface properties (shape and charge o ...
Text S1.
... “For the smaller non-polar residues this number is equal to the maximum area loss that could occur in going from an isolated α-helix to a fully buried environment in the complex. For the larger non-polar residues only a fraction of this value is used since they can never be totally buried in a singl ...
... “For the smaller non-polar residues this number is equal to the maximum area loss that could occur in going from an isolated α-helix to a fully buried environment in the complex. For the larger non-polar residues only a fraction of this value is used since they can never be totally buried in a singl ...
Cold-Shock Response in Microorganisms
... temperature, and the cellular response to this is termed the “cold-shock response."Earth’s biosphere ranges in temperature from above 100 °C to below 0 °C. However, the bulk of the biosphere is cold (e.g., most of the ocean, alpine regions, and natural environments exposed to the winter season, as w ...
... temperature, and the cellular response to this is termed the “cold-shock response."Earth’s biosphere ranges in temperature from above 100 °C to below 0 °C. However, the bulk of the biosphere is cold (e.g., most of the ocean, alpine regions, and natural environments exposed to the winter season, as w ...
¹⁵N Heteronuclear Single Quantum Coherence
... The abundance of this protein makes it a logical choice for the investigation of protein structure and function relationships. It is similar in structure to hemoglobin, but is smaller and less complex since it exists as a monomer instead of a heterotetramer. Myoglobin has been used as a model to stu ...
... The abundance of this protein makes it a logical choice for the investigation of protein structure and function relationships. It is similar in structure to hemoglobin, but is smaller and less complex since it exists as a monomer instead of a heterotetramer. Myoglobin has been used as a model to stu ...
RFLP Lab Report
... by restriction enzymes, “sticky” or “blunt” ends are formed. Sticky ends occur when singlestranded regions of the ends are complementary, and blunt ends occur when cut are opposite each other. The size of DNA fragments generated depends on the distance between recognition sites. Though RFLP analysis ...
... by restriction enzymes, “sticky” or “blunt” ends are formed. Sticky ends occur when singlestranded regions of the ends are complementary, and blunt ends occur when cut are opposite each other. The size of DNA fragments generated depends on the distance between recognition sites. Though RFLP analysis ...
Electrophoresisppt
... The porous material is made of microscopic particles suspended in a gel. The microscopic particles attach to one another forming tunnels that act as a sieve to separate the molecules. Small molecules can move faster than large molecules. ...
... The porous material is made of microscopic particles suspended in a gel. The microscopic particles attach to one another forming tunnels that act as a sieve to separate the molecules. Small molecules can move faster than large molecules. ...
1055 BIOTECHNOLOGYDERIVED ARTICLES—PEPTIDE
... involves the chemical or enzymatic treatment of a protein, resulting in the formation of peptide fragments, followed by separation and identification of the resultant fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from eve ...
... involves the chemical or enzymatic treatment of a protein, resulting in the formation of peptide fragments, followed by separation and identification of the resultant fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from eve ...
proteins
... • rich in other B-complex vitamins and lactose • dried whey is available in shops (esp. fitness centres) ...
... • rich in other B-complex vitamins and lactose • dried whey is available in shops (esp. fitness centres) ...
Lesson 4.Protein
... hydrogen atom (Fig. 4.1; in glycine, the R group is another hydrogen atom). The α-carbon atom is thus a chiral center. Because of the tetrahedral arrangement of the bonding orbitals around the α-carbon atom, the four different groups can occupy two unique spatial arrangements, and thus amino acids h ...
... hydrogen atom (Fig. 4.1; in glycine, the R group is another hydrogen atom). The α-carbon atom is thus a chiral center. Because of the tetrahedral arrangement of the bonding orbitals around the α-carbon atom, the four different groups can occupy two unique spatial arrangements, and thus amino acids h ...
1259-1260. Yeast as a model organism.
... accumulation of knowledge on all the proteins of an eukaryotic cell, the yeast Saccharomyces cerevisiae. This yeast is the most thoroughly studied living organism, with over 3000 of Its 6000 proteins characterized by either biochemistry or genetics or both. The wealth of knowledge on these proteins ...
... accumulation of knowledge on all the proteins of an eukaryotic cell, the yeast Saccharomyces cerevisiae. This yeast is the most thoroughly studied living organism, with over 3000 of Its 6000 proteins characterized by either biochemistry or genetics or both. The wealth of knowledge on these proteins ...
`Hybrid Protein Model`for optimally defining 3D protein structure
... may adopt only a limited number of folds (Govindarajan et al., 1999), the determination of their 3D structure from their sequence remains a difficult task (Baker and Sali, 2001). The conventional methods, such as homology modeling (Fiser and Sali, 2002) and threading (Kelley et al., 2000) take advan ...
... may adopt only a limited number of folds (Govindarajan et al., 1999), the determination of their 3D structure from their sequence remains a difficult task (Baker and Sali, 2001). The conventional methods, such as homology modeling (Fiser and Sali, 2002) and threading (Kelley et al., 2000) take advan ...
Exploring Mouse Protein Function via Multiple Approaches
... novel approach to predict mouse protein functions. The approach was a sequential combination of a similarity-based approach, an interaction-based approach and a pseudo amino acid composition-based approach. The method achieved an accuracy of about 0.8450 for the 1st-order predictions in the leave-on ...
... novel approach to predict mouse protein functions. The approach was a sequential combination of a similarity-based approach, an interaction-based approach and a pseudo amino acid composition-based approach. The method achieved an accuracy of about 0.8450 for the 1st-order predictions in the leave-on ...
Document
... grounding system. EnzyMiner was used as development corpus. • originally created for mutation impact extraction • it only contains information about mutations whose impact is studied • there may be other mutations associated with specific proteins but not with impacts • we only compute our performan ...
... grounding system. EnzyMiner was used as development corpus. • originally created for mutation impact extraction • it only contains information about mutations whose impact is studied • there may be other mutations associated with specific proteins but not with impacts • we only compute our performan ...
PTC Taster Lab Student`s Guide
... up to one day ahead of the second period and stored in a refrigerator, covered in plastic wrap and protected from light. 1. Prepare a clean and dry agarose gel casting tray ● Seal off the ends of the tray as indicated for your apparatus (not needed for blueGel™ users) ● Place a well-forming comb at ...
... up to one day ahead of the second period and stored in a refrigerator, covered in plastic wrap and protected from light. 1. Prepare a clean and dry agarose gel casting tray ● Seal off the ends of the tray as indicated for your apparatus (not needed for blueGel™ users) ● Place a well-forming comb at ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
... Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubilized immediately after its purification, or whether it was first washed with acetone and dried as described above (see 'M ...
... Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubilized immediately after its purification, or whether it was first washed with acetone and dried as described above (see 'M ...
Studying Protein Flexibility in a Statistical Framework: Tools and
... For fragment and domain protein motions the database systematizes the motions on the basis of the packing of atoms inside of proteins, which is a fundamental constraint on protein structure29,34-42. Interfaces between different parts of a protein are usually packed very tightly. Consequently, two ba ...
... For fragment and domain protein motions the database systematizes the motions on the basis of the packing of atoms inside of proteins, which is a fundamental constraint on protein structure29,34-42. Interfaces between different parts of a protein are usually packed very tightly. Consequently, two ba ...