simulating protein analysis using gel electrophoresis
... 4. A current of 200 volts is passed through the electrodes for (30) minutes. This current will cause the negatively charged proteins to move through the gel towards the positively charged electrode. The proteins will migrate at a known rate based on their size, with the smaller proteins moving a gre ...
... 4. A current of 200 volts is passed through the electrodes for (30) minutes. This current will cause the negatively charged proteins to move through the gel towards the positively charged electrode. The proteins will migrate at a known rate based on their size, with the smaller proteins moving a gre ...
37151
... Proteomics is usually carried out to study the complement of protein expressed by a cell at any one time or at a particular stage ...
... Proteomics is usually carried out to study the complement of protein expressed by a cell at any one time or at a particular stage ...
A-Ag
... Gel electrophoresis is a procedure that separates molecules on the basis of their rate of movement through a gel under the influence of an electrical field. We will be using agarose gel electrophoresis to determine the presence and size of PCR products. ...
... Gel electrophoresis is a procedure that separates molecules on the basis of their rate of movement through a gel under the influence of an electrical field. We will be using agarose gel electrophoresis to determine the presence and size of PCR products. ...
Protein Purification and Characterization Techniques
... • Separation on the basis of size (MW) • Stationary (cross-linked gel particles): consist of one of two kinds of polymers; the 1st is a carb. polymer (ex. dextran or agarose); often referred to by Sephadex and Sepharose. The 2nd is based on polyacrylamide (Bio-Gel) • Extent of crosslinking & pore si ...
... • Separation on the basis of size (MW) • Stationary (cross-linked gel particles): consist of one of two kinds of polymers; the 1st is a carb. polymer (ex. dextran or agarose); often referred to by Sephadex and Sepharose. The 2nd is based on polyacrylamide (Bio-Gel) • Extent of crosslinking & pore si ...
(Western) Blotting
... Cells are grown to desired density (OD) Samples are centrifuged to collect cells and separate media (discard supernatant) Wash samples in buffer to remove salts Coat samples in SDS-loading buffer Boil samples for 5 minutes to denature proteins ...
... Cells are grown to desired density (OD) Samples are centrifuged to collect cells and separate media (discard supernatant) Wash samples in buffer to remove salts Coat samples in SDS-loading buffer Boil samples for 5 minutes to denature proteins ...
Prezentace aplikace PowerPoint
... • The location of the antibody is revealed by incubating it with a substrate that the attached enzyme converts to a product that can be seen and followed and then photographed. ...
... • The location of the antibody is revealed by incubating it with a substrate that the attached enzyme converts to a product that can be seen and followed and then photographed. ...
Proteomics techniques used to identify proteins
... Identification of regulatory proteins from human cells using 2D-GE and LC-MS/MS Victor Paromov Christian Muenyi William L. Stone ...
... Identification of regulatory proteins from human cells using 2D-GE and LC-MS/MS Victor Paromov Christian Muenyi William L. Stone ...
Gel Electrophoresis
... * Innovative ideas- do science at home * Think outside the textbook realm * Molecular biology = very important for new ideas ...
... * Innovative ideas- do science at home * Think outside the textbook realm * Molecular biology = very important for new ideas ...
The Nobel Prize in Chemistry 1948 Arne Tiselius
... TESS The Nobel Prize in Chemistry 1948 Arne Tiselius ...
... TESS The Nobel Prize in Chemistry 1948 Arne Tiselius ...
charge-to-mass ratio. The electrophoretic mobility is defined as the
... similarity between the above equation and that used for gel filtration. For example, if hemoglobin were run as a standard, it would result in a band on the gel at a mobility corresponding to Mr = 16 kDa, i.e. its monomer molecular weight and myoglobin (Mr = 17 kDa) would be nearby because it is a si ...
... similarity between the above equation and that used for gel filtration. For example, if hemoglobin were run as a standard, it would result in a band on the gel at a mobility corresponding to Mr = 16 kDa, i.e. its monomer molecular weight and myoglobin (Mr = 17 kDa) would be nearby because it is a si ...
No Slide Title
... Gel Electrophoresis native: mobility = (voltage)(charge)/(mass) SDS-PAGE: minimizes contribution of charge IEF: minimizes contribution of size Isoelectric Focusing • separates proteins by isoelectric points • large pore size of gel and equilibrium conditions minimize molecular sieving • native or de ...
... Gel Electrophoresis native: mobility = (voltage)(charge)/(mass) SDS-PAGE: minimizes contribution of charge IEF: minimizes contribution of size Isoelectric Focusing • separates proteins by isoelectric points • large pore size of gel and equilibrium conditions minimize molecular sieving • native or de ...
Polyacrylamide gels
... Bis acrylamide is a cross linking agent and links long polymers of acrylamide (N, N’-methylene bisacrylamide) Pore size is determined by % acrylamide and the amount of cross linker The copolymerization of acrylamide with methylenebisacrylamide produces a mesh-like network in three dimensions, consis ...
... Bis acrylamide is a cross linking agent and links long polymers of acrylamide (N, N’-methylene bisacrylamide) Pore size is determined by % acrylamide and the amount of cross linker The copolymerization of acrylamide with methylenebisacrylamide produces a mesh-like network in three dimensions, consis ...