The Mac Daddies of Molecules
... They are fats,steroids,oils and waxes Examples are margarine, shortening, meats, olive oil, peanut oil Lipids are used for storing energy (why it pays to have some fat on you!) ...
... They are fats,steroids,oils and waxes Examples are margarine, shortening, meats, olive oil, peanut oil Lipids are used for storing energy (why it pays to have some fat on you!) ...
LAB SESSION 1: Bioprocessing
... Within the gel column, students can visually watch as the GFP is separated from the other proteins. They will learn techniques used to quantify the amount of protein as well as the purification technique used. Students will gain an understanding of the usefulness of both the techniques and the uniqu ...
... Within the gel column, students can visually watch as the GFP is separated from the other proteins. They will learn techniques used to quantify the amount of protein as well as the purification technique used. Students will gain an understanding of the usefulness of both the techniques and the uniqu ...
LAB SESSION 1: Bioprocessing
... Within the gel column students can visually watch as the GFP is separated from the other proteins. They will learn techniques used to quantify the amount of protein as well as the purification technique used. Students will gain an understanding of the usefulness of both the techniques and the unique ...
... Within the gel column students can visually watch as the GFP is separated from the other proteins. They will learn techniques used to quantify the amount of protein as well as the purification technique used. Students will gain an understanding of the usefulness of both the techniques and the unique ...
Quiz Next Tuesday (09/18) - Chemistry at Winthrop University
... NH3+ groups of two amino acids can react with the resulting loss of a water molecule to form a ...
... NH3+ groups of two amino acids can react with the resulting loss of a water molecule to form a ...
PDF
... gradient polyacrylamide gel [5]. Another useful technique is Differential In-Gel Electrophoresis (DIGE) which is when proteins labeled with up to three different fluorescent dyes, run on the same 2-D gel and are individually scanned at appropriate fluorescent wavelengths [6]. Mass spectrometry (MS) ...
... gradient polyacrylamide gel [5]. Another useful technique is Differential In-Gel Electrophoresis (DIGE) which is when proteins labeled with up to three different fluorescent dyes, run on the same 2-D gel and are individually scanned at appropriate fluorescent wavelengths [6]. Mass spectrometry (MS) ...
Affinity Chromatography
... Affinity chromatography is a powerful technique to purify one component out of highly complex mixtures. It is based on the interaction of two molecules (biotin-avidin). One is fixed on the column, while the other is selected out of the mixture. A special form of affinity chromatography is metal chel ...
... Affinity chromatography is a powerful technique to purify one component out of highly complex mixtures. It is based on the interaction of two molecules (biotin-avidin). One is fixed on the column, while the other is selected out of the mixture. A special form of affinity chromatography is metal chel ...
ELECTROPHORESIS
... helping further resolution. The two different porosity gels are used 1. Stacking gel ( high porosity) 2. Separating or running gel (low porosity). ...
... helping further resolution. The two different porosity gels are used 1. Stacking gel ( high porosity) 2. Separating or running gel (low porosity). ...
Dr Asmat Salim MM707-electrophoresis 2014
... In the biological system, many molecules are electrically charged and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape ...
... In the biological system, many molecules are electrically charged and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape ...
Supporting Information File SF5
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
Electrophoresis
... Load samples by pipetting slowly through buffer into wells Attach leads to tank and power supply; set V so I < 60 mA Agarose concentration needed is determined by sizes of DNA fragments to be separated o for fragments between 0.3 and 3 kb, 0.7-1% are good standard concentrations o to separate smal ...
... Load samples by pipetting slowly through buffer into wells Attach leads to tank and power supply; set V so I < 60 mA Agarose concentration needed is determined by sizes of DNA fragments to be separated o for fragments between 0.3 and 3 kb, 0.7-1% are good standard concentrations o to separate smal ...
TD7: Gel Electrophoresis Photoaffinity probes GEL
... TD7: Gel Electrophoresis Photoaffinity probes GEL ELECTROPHORESIS ...
... TD7: Gel Electrophoresis Photoaffinity probes GEL ELECTROPHORESIS ...
Mass Spectrometers - Porto Conte Ricerche
... MALDI (Matrix Assisted Laser Desorption Ionization) source provides accurate information on the structure and molecular weight of biomolecules such as peptides, proteins, oligonucleotides and carbohydrates, as well as synthetic polymers. Furthermore, through the PMF (Peptide MassFingerprinting) tech ...
... MALDI (Matrix Assisted Laser Desorption Ionization) source provides accurate information on the structure and molecular weight of biomolecules such as peptides, proteins, oligonucleotides and carbohydrates, as well as synthetic polymers. Furthermore, through the PMF (Peptide MassFingerprinting) tech ...
Student worksheet for Proteins
... Strand: Molecules and Cells Name___________________________ Date__________________ School_________________ Student—please print this worksheet and complete it as you interact with the tutorial. The completed worksheet should be turned in to your assigned teacher. Tutorial: Proteins Protein Definitio ...
... Strand: Molecules and Cells Name___________________________ Date__________________ School_________________ Student—please print this worksheet and complete it as you interact with the tutorial. The completed worksheet should be turned in to your assigned teacher. Tutorial: Proteins Protein Definitio ...
Acrylamide -gel patterns of total soluble proteins at different stages +
... From cultures of synchronously developing larvae, samples were collected at different times of development. Larvae were collected in 2 M sucrose, washed twice in Tris-EDTA-Borate buffer and carefully dried on kleenex tissues. The total soluble proteins of the larvae and the hemolymph specific protei ...
... From cultures of synchronously developing larvae, samples were collected at different times of development. Larvae were collected in 2 M sucrose, washed twice in Tris-EDTA-Borate buffer and carefully dried on kleenex tissues. The total soluble proteins of the larvae and the hemolymph specific protei ...
Pre-lab 1 and Lab 1 2010 - Sonoma Valley High School
... Genetic engineering allows humans to insert human DNA into other organisms and then have these genetically modified organisms make human proteins. These proteins can be used to treat a wide variety of diseases and help millions of people. The sequence of labs in the Amgen Biotech Experience mimics t ...
... Genetic engineering allows humans to insert human DNA into other organisms and then have these genetically modified organisms make human proteins. These proteins can be used to treat a wide variety of diseases and help millions of people. The sequence of labs in the Amgen Biotech Experience mimics t ...
pH of the electrophoresis buffer
... Molecules move towards opposite polarity under current • Settle at Isoelectric point :Molecule no longer has a net electric charge (due to the protonation or deprotonation of the associated functional groups) ...
... Molecules move towards opposite polarity under current • Settle at Isoelectric point :Molecule no longer has a net electric charge (due to the protonation or deprotonation of the associated functional groups) ...
Protein Electrophoresis
... to accurately separate proteins by molecular weight and not by shape or charge, the secondary structure of the protein is unfolded using the anionic detergent sodium dodecyl sulfate (SDS) and a reducing agent. The SDS molecules form a complex with the protein, negating its inherent charge. The reduc ...
... to accurately separate proteins by molecular weight and not by shape or charge, the secondary structure of the protein is unfolded using the anionic detergent sodium dodecyl sulfate (SDS) and a reducing agent. The SDS molecules form a complex with the protein, negating its inherent charge. The reduc ...
COA: phiX174 DNA/BsuRI (HaeIII) Marker, 9, ready-to
... Description ΦX174 DNA/BsuRI (HaeIII) Marker, 9, is premixed with DNA Loading Dye at a total DNA concentration of 0.1 µg/µl and can be directly applied onto agarose or polyacrylamide gels. The Marker contains the following 11 discrete fragments (in base pairs): 1353, 1078, 872, 603, 310, 281, 271, ...
... Description ΦX174 DNA/BsuRI (HaeIII) Marker, 9, is premixed with DNA Loading Dye at a total DNA concentration of 0.1 µg/µl and can be directly applied onto agarose or polyacrylamide gels. The Marker contains the following 11 discrete fragments (in base pairs): 1353, 1078, 872, 603, 310, 281, 271, ...
ELECTROPHORESIS
... Factors affecting the distance of movement 1- Net charge on the molecule: Particles with negative net charges move toward the anode (positive pole) where as particles with positive charges migrate toward the cathode (negative pole). 2- Size and shape of the molecule: Particles of identical net charg ...
... Factors affecting the distance of movement 1- Net charge on the molecule: Particles with negative net charges move toward the anode (positive pole) where as particles with positive charges migrate toward the cathode (negative pole). 2- Size and shape of the molecule: Particles of identical net charg ...
Hortmon and Udenfriend (I969 Anal. ... Fisher, C. R. Anilinonophthalene rulfonote ...
... system produced by ORTEC has prov~ided superior resolution of Neurorenzyme activity after electrophoresir. pore proteins, but recovery of SAICAR rynthetore (Fisher 1969 Biochim. Biophyr. Ac+o 178:380) activity has been quite low. The dam of Fonhr and Furminger (1967 Nature 215:750) indicated that th ...
... system produced by ORTEC has prov~ided superior resolution of Neurorenzyme activity after electrophoresir. pore proteins, but recovery of SAICAR rynthetore (Fisher 1969 Biochim. Biophyr. Ac+o 178:380) activity has been quite low. The dam of Fonhr and Furminger (1967 Nature 215:750) indicated that th ...
1.16891 / 1.16886 Fractogel® EMD COO
... Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and chemical stability. Since the functional ion excha ...
... Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and chemical stability. Since the functional ion excha ...
Document
... • DNA is negatively charged • Smaller sized DNA moves faster than Larger DNA • Markers are used to determine relative sizes of DNA pieces ...
... • DNA is negatively charged • Smaller sized DNA moves faster than Larger DNA • Markers are used to determine relative sizes of DNA pieces ...