Review Structural glycobiology: A game of snakes and ladders
... Thus far, only rarely has the design of carbohydrate-based therapeutic agents made extensive use of 3D structural information, reflecting in part the difficulties of determining carbohydrate conformation, as well as a paucity of structural data for many carbohydrate—protein complexes. To help revers ...
... Thus far, only rarely has the design of carbohydrate-based therapeutic agents made extensive use of 3D structural information, reflecting in part the difficulties of determining carbohydrate conformation, as well as a paucity of structural data for many carbohydrate—protein complexes. To help revers ...
Contribution of defined amino acid residues to the immunogenicity
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
application of hydroxyapatite in protein purification
... The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700oC of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is ...
... The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700oC of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is ...
Bacterial Screening PCR Kit
... In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control appli ...
... In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control appli ...
Identification of Novel microRNA Regulatory Proteins in Neurons
... siRNAs affect 3’UTR–dependent translation in that the identified RBPs seem to play a more general a more general fashion. To test this hypothesis, role in miR-134–dependent translational control. we repeated the Luciferase assay for the identified candidates using a Luciferase reporter construct wit ...
... siRNAs affect 3’UTR–dependent translation in that the identified RBPs seem to play a more general a more general fashion. To test this hypothesis, role in miR-134–dependent translational control. we repeated the Luciferase assay for the identified candidates using a Luciferase reporter construct wit ...
Adaptative biochemical pathways and regulatory networks in
... drainages, which represents one of the major elements to make rust-red the acidic waters. In this case, iron can represent an environmental hazard for life, especially for its oxidative properties and for the presence of other metals which increase the total toxicity of mine drainages and cause a si ...
... drainages, which represents one of the major elements to make rust-red the acidic waters. In this case, iron can represent an environmental hazard for life, especially for its oxidative properties and for the presence of other metals which increase the total toxicity of mine drainages and cause a si ...
ENZYMES
... The Chemical nature of enzymes • Enzymes are globular proteins. They have a complex tertiary and quaternary structure in which polypeptides are folded around each other to form a roughly spherical or globular shape. The overall 3D shape of an enzyme molecule is very important: if it is altered, the ...
... The Chemical nature of enzymes • Enzymes are globular proteins. They have a complex tertiary and quaternary structure in which polypeptides are folded around each other to form a roughly spherical or globular shape. The overall 3D shape of an enzyme molecule is very important: if it is altered, the ...
Jacob Corn
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
AMINO ACIDS, PEPTIDES, AND PROTEINS
... roteins are the most abundant biological macromolecules, occurring in all cells and all parts of cells. Proteins also occur in great variety; thousands of different kinds, ranging in size from relatively small peptides to huge polymers with molecular weights in the millions, may be found in a single ...
... roteins are the most abundant biological macromolecules, occurring in all cells and all parts of cells. Proteins also occur in great variety; thousands of different kinds, ranging in size from relatively small peptides to huge polymers with molecular weights in the millions, may be found in a single ...
Amino Acids, Peptides, and Proteins
... bonds between their amino acids. Similarly, the special functions of polysaccharides, nucleic acids, and lipids can be understood as a direct manifestation of their chemical structure, with their characteristic monomeric subunits linked in precise functional polymers. Sugars linked together become e ...
... bonds between their amino acids. Similarly, the special functions of polysaccharides, nucleic acids, and lipids can be understood as a direct manifestation of their chemical structure, with their characteristic monomeric subunits linked in precise functional polymers. Sugars linked together become e ...
Full-Text PDF
... PIG-L domain (Pfam [2] PF02585; Table 1). The PIG-L domain name itself comes from the enzyme N-acetyl-D-glucosaminylphosphatidylinositol deacetylase (PIG-L), one of the initial members of this family to be identified [3]. Although the structure of PIG-L has not been reported, the structures of five ...
... PIG-L domain (Pfam [2] PF02585; Table 1). The PIG-L domain name itself comes from the enzyme N-acetyl-D-glucosaminylphosphatidylinositol deacetylase (PIG-L), one of the initial members of this family to be identified [3]. Although the structure of PIG-L has not been reported, the structures of five ...
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives
... complexes (SWI2/SNF2, SWR1, HAT). Conventional actins and ARPs together define a large family of homologous proteins, the actin superfamily, with a tertiary structure known as the “actin fold”. Since 1997 (Poch and Winsor), the unified classification of ARPs is composed of 11 families, based primari ...
... complexes (SWI2/SNF2, SWR1, HAT). Conventional actins and ARPs together define a large family of homologous proteins, the actin superfamily, with a tertiary structure known as the “actin fold”. Since 1997 (Poch and Winsor), the unified classification of ARPs is composed of 11 families, based primari ...
Relationship Between the Occurrence of Cysteine in Proteins and
... two other amino acids (C-(X)2-C motif; X may stand for any coded amino acid). Indeed, it is known that C-(X)2C motifs are well conserved parts of various iron-sulfur proteins and oxidoreductases (Shuber et al. 1986; Ammendola et al. 1992). However, we recognized that the occurrence of C-(X)2-C domai ...
... two other amino acids (C-(X)2-C motif; X may stand for any coded amino acid). Indeed, it is known that C-(X)2C motifs are well conserved parts of various iron-sulfur proteins and oxidoreductases (Shuber et al. 1986; Ammendola et al. 1992). However, we recognized that the occurrence of C-(X)2-C domai ...
Biological Networks Underlying Abiotic Stress Tolerance in
... proteome level, especially when using 2DE based approaches, alterations in protein abundance are scarcely detected due to a relatively low abundance of signaling proteins with respect to other cellular proteins and due to the rapidity of their changes during an alarm phase of stress response. Howeve ...
... proteome level, especially when using 2DE based approaches, alterations in protein abundance are scarcely detected due to a relatively low abundance of signaling proteins with respect to other cellular proteins and due to the rapidity of their changes during an alarm phase of stress response. Howeve ...
Effect of Six Decades of Selective Breeding on
... had not varied substantially from the ancestral lines to the present commercial cultivars. There was noticeably less β-subunit of β-conglycinin, a protein devoid of sulfur amino acids, in the modern cultivars represented by Mustang, Pioneer 93B09, and Asgrow 3602. Comparison of the amino acid profil ...
... had not varied substantially from the ancestral lines to the present commercial cultivars. There was noticeably less β-subunit of β-conglycinin, a protein devoid of sulfur amino acids, in the modern cultivars represented by Mustang, Pioneer 93B09, and Asgrow 3602. Comparison of the amino acid profil ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... sulphate or by kethoxal. The sites of chemical modification and the drug protection site(s) are detected by isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carri ...
... sulphate or by kethoxal. The sites of chemical modification and the drug protection site(s) are detected by isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carri ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
... conserved among pathogenic bacteria. It has been identified as a sugar isomerase with specificity for the rare sugar D-lyxose as well as D-mannose based on structural homology to the cupin phosphoglucose isomerases, suggesting a role for EcSI in metabolism of alternative carbon sources. Structural h ...
... conserved among pathogenic bacteria. It has been identified as a sugar isomerase with specificity for the rare sugar D-lyxose as well as D-mannose based on structural homology to the cupin phosphoglucose isomerases, suggesting a role for EcSI in metabolism of alternative carbon sources. Structural h ...
Recombinant expression and characterisation of monofunctional S-
... E. coli and this diminishes the expression levels of the recombinant proteins (Baca and Ho~ 2000; Sayers, et al., 1995). One method to overcome the codon bias is to reengineer the Plasmodium gene by synthesising the gene so that it uses the preferred codons of E. coli (Pan, et ai., 1999; Prapunwatta ...
... E. coli and this diminishes the expression levels of the recombinant proteins (Baca and Ho~ 2000; Sayers, et al., 1995). One method to overcome the codon bias is to reengineer the Plasmodium gene by synthesising the gene so that it uses the preferred codons of E. coli (Pan, et ai., 1999; Prapunwatta ...
Free amino acids and proteins dynamics in somatic embryogenesis
... proteins. The residual was retaken two times in the previous buffer for 20 min with at each time centrifugation at 6000 gn for 20 min and elimination of the supernatant. The residual was mixed and incubated in 1 ml cold Trismaleate buffer 0.01M, pH 7 containing sodium chloride 1M at 4 °C during 40 m ...
... proteins. The residual was retaken two times in the previous buffer for 20 min with at each time centrifugation at 6000 gn for 20 min and elimination of the supernatant. The residual was mixed and incubated in 1 ml cold Trismaleate buffer 0.01M, pH 7 containing sodium chloride 1M at 4 °C during 40 m ...
ENZYMES
... They do this by lowering activation energy. They create a new reaction pathway “a short cut” ...
... They do this by lowering activation energy. They create a new reaction pathway “a short cut” ...
Characterization of Low Molecular Weight Glutenin Subunits by
... sequences of the faster-moving C group of LMW-GS corresponded mainly to y-type and a-type gliadins. These gliadinlike C subunits presumably resulted from gliadin genes that acquired an extra cysteine residue due to a point mutation; the resulting odd number of cysteines necessitates the incorporatio ...
... sequences of the faster-moving C group of LMW-GS corresponded mainly to y-type and a-type gliadins. These gliadinlike C subunits presumably resulted from gliadin genes that acquired an extra cysteine residue due to a point mutation; the resulting odd number of cysteines necessitates the incorporatio ...
Complex forming reactions and complexometry Complex forming
... salt. The titrant needs to be standardized due to its high affinity towards metal ions. The standardization can be done by using e.g. a standard Zn(II) solution, either prepared from the pure metal or from its salt. Due to metallic impurities in glass containers, plastic containers are recommended f ...
... salt. The titrant needs to be standardized due to its high affinity towards metal ions. The standardization can be done by using e.g. a standard Zn(II) solution, either prepared from the pure metal or from its salt. Due to metallic impurities in glass containers, plastic containers are recommended f ...
NIH Public Access
... cThese authors contributed equally to this work. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and ...
... cThese authors contributed equally to this work. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and ...
Proteases of Senescing Oat Leaves
... endoprotease, while the neutral one is an exoprotease. Evidence is pre- point, and omission of the DEAE step avoids the large loss of sented that these proteases undergo autolysis in vitro. activity normally encountered at the final stage; the primary disadvantage is that they are not separated from ...
... endoprotease, while the neutral one is an exoprotease. Evidence is pre- point, and omission of the DEAE step avoids the large loss of sented that these proteases undergo autolysis in vitro. activity normally encountered at the final stage; the primary disadvantage is that they are not separated from ...