Probing peroxisomal β-oxidation and the labelling of acetyl
... studies had concluded that the labelling of liver mitochondrial acetyl-CoA is not homogeneous [23,24]. However, to our knowledge of the literature, this is the first demonstration of the influence of the concentration of a labelled acetyl-CoA precursor on the labelling homogeneity of mitochondrial a ...
... studies had concluded that the labelling of liver mitochondrial acetyl-CoA is not homogeneous [23,24]. However, to our knowledge of the literature, this is the first demonstration of the influence of the concentration of a labelled acetyl-CoA precursor on the labelling homogeneity of mitochondrial a ...
Ketone body metabolism and cardiovascular disease - AJP
... Fig. 1. Ketone body metabolism pathways. A: ketogenesis within hepatic mitochondria is the primary source of circulating ketone bodies. B: primary metabolic fate of ketone bodies is terminal oxidation within mitochondria of extrahepatic tissues via CoA transferase [succinyl-CoA:3-oxoacid-CoA transfe ...
... Fig. 1. Ketone body metabolism pathways. A: ketogenesis within hepatic mitochondria is the primary source of circulating ketone bodies. B: primary metabolic fate of ketone bodies is terminal oxidation within mitochondria of extrahepatic tissues via CoA transferase [succinyl-CoA:3-oxoacid-CoA transfe ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... interaction in L12 dimer, had no functional relevance and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupy ...
... interaction in L12 dimer, had no functional relevance and was contradicted by an NMR structure where both the hinges were seen in fully extended form (9,11). Thus it is now universally accepted that the L12 dimer is ‘antiparallel’ where NTDs of two L12 molecules form a four-helix bundle dimer occupy ...
SUPPORTING FILE S1 SUPPLEMENTARY MATERIAL AND
... were washed twice with 0.9 % NaCl, frozen in liquid nitrogen and then lyophilised overnight. ...
... were washed twice with 0.9 % NaCl, frozen in liquid nitrogen and then lyophilised overnight. ...
Influence of Aerobic and Phototrophic Growth
... Federal Republic of Germany (Received I I February 1977) ...
... Federal Republic of Germany (Received I I February 1977) ...
Influence of Aerobic and Phototrophic Growth
... Federal Republic of Germany (Received I I February 1977) ...
... Federal Republic of Germany (Received I I February 1977) ...
Full-Text PDF
... generated as the unintended side products of the electron transport chain that generates ATP. As such, while the generation of ATP makes the mitochondria obligatory for survival and life, the generation of reactive oxygen species that damage cellular macromolecules such as DNA/RNA, proteins, and lip ...
... generated as the unintended side products of the electron transport chain that generates ATP. As such, while the generation of ATP makes the mitochondria obligatory for survival and life, the generation of reactive oxygen species that damage cellular macromolecules such as DNA/RNA, proteins, and lip ...
Global transcriptional control by glucose and
... of the virulence genes is an important step of the pathogenicity process. In C. difficile, toxin production increases as cells enter into stationary phase and is modulated in response to different environmental signals, such as temperature, biotin limitation, the presence of antibiotics, of butyric a ...
... of the virulence genes is an important step of the pathogenicity process. In C. difficile, toxin production increases as cells enter into stationary phase and is modulated in response to different environmental signals, such as temperature, biotin limitation, the presence of antibiotics, of butyric a ...
Developement of strategies for the isotopic labeling of methyl
... enhances the intensity of detectable long-range nOes by a factor 4. The described protocol also permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using thi ...
... enhances the intensity of detectable long-range nOes by a factor 4. The described protocol also permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using thi ...
Advances in Chemical Protein Modification
... methods to preferentially modify primary amines (although often these methods also modify the N-terminus).18 These protocols involve direct reaction with electrophilic reagents such as activated acids, vinyl sulfones, sulfonyl chlorides, iso(thio)cyanates, and squaric acids as well as reductive amin ...
... methods to preferentially modify primary amines (although often these methods also modify the N-terminus).18 These protocols involve direct reaction with electrophilic reagents such as activated acids, vinyl sulfones, sulfonyl chlorides, iso(thio)cyanates, and squaric acids as well as reductive amin ...
The Proposed Effects of Nicotinamide Adenine Dinucleotide (NAD
... delivery is only limiting at VO2max where beyond this point oxygen uptake and delivery will become limiting. With regards to the majority of sporting events, exercise is carried out sub maximally for the athlete and so oxygen supply will not be limiting. An early experiment concluded that it seems u ...
... delivery is only limiting at VO2max where beyond this point oxygen uptake and delivery will become limiting. With regards to the majority of sporting events, exercise is carried out sub maximally for the athlete and so oxygen supply will not be limiting. An early experiment concluded that it seems u ...
Targeting apicoplasts in malaria parasites
... had been lost, but it was confirmed that the apicoplast carried four identifiable metabolic pathways: isoprenoid precursor synthesis, fatty acid synthesis, heme synthesis and ironsulfur cluster biogenesis. Also present were the components of the ‘housekeeping’ functions of genome replication, transc ...
... had been lost, but it was confirmed that the apicoplast carried four identifiable metabolic pathways: isoprenoid precursor synthesis, fatty acid synthesis, heme synthesis and ironsulfur cluster biogenesis. Also present were the components of the ‘housekeeping’ functions of genome replication, transc ...
From Sequence to Structure
... titratable –N–H groups, each with pKa values around 6. When one of these –N–H groups loses a proton, however, the pKa of the other one becomes much greater than 10. When both are protonated, the residue as a whole is positively charged. When only one is protonated (usually it is the one farthest fro ...
... titratable –N–H groups, each with pKa values around 6. When one of these –N–H groups loses a proton, however, the pKa of the other one becomes much greater than 10. When both are protonated, the residue as a whole is positively charged. When only one is protonated (usually it is the one farthest fro ...
COMPARATIVE HYDROLYSIS OF GELATIN BY PEPSIN, TRYPSIN
... reaction was similar with pepsin and acid, and with trypsin and alkali. The study of the hydrolytic products of trypsin and pepsin leads to the same conclusion? I t is usually stated that trypsin hydrolysis leads to more amino-acid formation. More quantitative evidence may be obtained, however, by a ...
... reaction was similar with pepsin and acid, and with trypsin and alkali. The study of the hydrolytic products of trypsin and pepsin leads to the same conclusion? I t is usually stated that trypsin hydrolysis leads to more amino-acid formation. More quantitative evidence may be obtained, however, by a ...
Aalborg Universitet metabolic engineering approach
... If you believe that this document breaches copyright please contact us at [email protected] providing details, and we will remove access to the work immediately and investigate your claim. ...
... If you believe that this document breaches copyright please contact us at [email protected] providing details, and we will remove access to the work immediately and investigate your claim. ...
Expression and activity of hexokinase in the early mouse embryo
... 1982). Hexokinase I has been the most extensively studied (largely in the rat) and is the only isoenzyme whose DNA sequence is available for mouse, derived from tumour tissue (Arora et al., 1990). The gene consists of two structural halves, both coding for proteins containing an ATP and a glucose bi ...
... 1982). Hexokinase I has been the most extensively studied (largely in the rat) and is the only isoenzyme whose DNA sequence is available for mouse, derived from tumour tissue (Arora et al., 1990). The gene consists of two structural halves, both coding for proteins containing an ATP and a glucose bi ...
Propionic Acid Degradation by Syntrophic Bacteria During
... phases in biogas production. Such perturbations should be avoided in order to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic ...
... phases in biogas production. Such perturbations should be avoided in order to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic ...
Journal of Applied Microbiology
... atypical E. c. atroseptica because, although they produce acid from a-methylglucoside and reducing substances from sucrose like E. c. atrospetica, they differ in having the ability to grow at 37C. Some of these strains, isolated from several hosts, including chicory, produce odorous volatile metabo ...
... atypical E. c. atroseptica because, although they produce acid from a-methylglucoside and reducing substances from sucrose like E. c. atrospetica, they differ in having the ability to grow at 37C. Some of these strains, isolated from several hosts, including chicory, produce odorous volatile metabo ...
Lecture 33 - University of Arizona
... Pathway Questions 3. What are the key enzymes in glycogen metabolism? Glycogen phosphorylase – enzyme catalyzing the phosphorylysis reaction that uses Pi to remove one glucose at a time from nonreducing ends of glycogen resulting in the formation of glucose-1P.. Glycogen synthase - enzyme catalyzin ...
... Pathway Questions 3. What are the key enzymes in glycogen metabolism? Glycogen phosphorylase – enzyme catalyzing the phosphorylysis reaction that uses Pi to remove one glucose at a time from nonreducing ends of glycogen resulting in the formation of glucose-1P.. Glycogen synthase - enzyme catalyzin ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.