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The Biochemistry of C 4 Photosynthesis
The Biochemistry of C 4 Photosynthesis

... 2. NAD-ME Type Bundle sheath chloroplasts of NAD-ME C4 species have thylakoid membranes with developed grana stackings. Both chloroplasts and mitochondria are located together in a centripetal position relative to the vascular bundle, except in some grass species of Panicum and Eragrostis (Ohsugi et ...
TETHERING: Fragment-Based Drug Discovery
TETHERING: Fragment-Based Drug Discovery

... NON-TETHERING METHODS Detecting fragments that bind a target can be challenging. Traditional high-throughput screening (HTS) typically relies on inhibition assays. But high concentrations of compound may be needed to find weak inhibitors. For example, an inhibitor with an IC50 of 1 mM produces less ...
Path of Glucose Breakdown and Cell Yields of a
Path of Glucose Breakdown and Cell Yields of a

... aconitase, and NADP-linked glutamic dehydrogenase were present in relatively high amounts in A . naeslundii (Table 2). Table 2. Demonstration of certain enzymes in cell-free extracts of Act inomyces naeslundii ...
Formate Dehydrogenase, Molecular Modeling and Docking with
Formate Dehydrogenase, Molecular Modeling and Docking with

... (Koeller and Wong, 2001; Schmid et al., 2001; Schoemaker et al., 2003). Cofactors such as NAD+ and NADH are compounds that are important for several enzymatic reactions. In recent years, researchers developed cofactor regeneration using many methods. Enzymatic reactions usually are the best choice f ...
Untitled
Untitled

... course guide is to provide you with clear description of the course objectives, contents of each topic together with its lectures, tutorials and practicals, which are presented in a sequential manner. Also it states clearly what is expected from you to achieve together with the evaluation procedures ...
World Index of BioMolecular Visualization Resources
World Index of BioMolecular Visualization Resources

... Cross-indexing terms: deoxyribonucleic acid, DNA, major groove, minor groove. This Chime resource contains animations to illustrate the structure of DNA. The animations are initiated by clicking buttons on the right pannel. They show a base pair in the context of a larger helix and highlight the bas ...
Protein Similarity Score - Santa Clara Law Digital Commons
Protein Similarity Score - Santa Clara Law Digital Commons

... this paper, I will focus on single chain proteins and make the simplifying assumption that all the amino acids that make up a protein are "standard" amino acids, i.e., selected from the group of twenty amino acids encoded by the standard genetic code. 7 Proteins can be defined structurally in terms ...
Note - EtoosIndia
Note - EtoosIndia

...  NADH2 & FADH2 obtained from glycolysis and Kreb's cycle enter in electron transport chain and form water molecule by oxidation with the help of molecular oxygen.  In this process, energy is released which is used for the synthesis of ATP.  Transfer of electrons from NADH2 or FADH2 occurs through ...
Metabolic Engineering of Tomato Fruit Organic
Metabolic Engineering of Tomato Fruit Organic

... transport of citrate between these subcellular compartments will also have a bearing on its rate of accumulation (Shiratake and Martinoia, 2007). Export from the mitochondrion is by counterexchange with other carboxylic acids that are imported. Therefore, the capacity of other parts of the TCA cycle ...
The role of the C8 proton of ATP in the catalysis of shikimate kinase
The role of the C8 proton of ATP in the catalysis of shikimate kinase

... binding and/or phosphoryl transfer within a range of kinase and synthetase enzymes. The role of the C8-H of ATP in the binding and/or phosphoryl transfer on the enzyme activity of a number of kinase and synthetase enzymes has been elucidated. The intrinsic catalysis rate mediated by each kinase enzy ...
Indexing Tricarboxylic Acid Cycle Flux in Intact Hearts by Carbon
Indexing Tricarboxylic Acid Cycle Flux in Intact Hearts by Carbon

... incorporated into the C-2 or C-3 position of fumarate, malate, and oxaloacetate. After the condensation of [2-13C]oxaloacetate or [3 -13C]oxaloacetate and acetyl-CoA, 13C is incorporated into the C-2 or C-3 of citrate and 2-oxoglutarate. Most citric acid cycle intermediates occur in concentrations t ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors

... coli (and in this organism only) [30]. It was shown by denaturing electrophoresis in polyacrylamide gel that NarZYV consists of two subunits with molecular mass of 150 and 60 kD encoded by narZ and narY genes and contains Moco. Subunit γ of NarZYV encoded by narV was lost during purification, but it ...
Regulation of Arabidopsis 14-3
Regulation of Arabidopsis 14-3

... GABA regulates 14-3-3 gene expression in Arabidopsis seedlings. Since 14-3-3 proteins regulate several aspects N metabolism in plants, we were interested to determine whether the 14-3-3s themselves might be subject to regulation by N metabolites. We therefore examined the expression of all members o ...
PDF File - Computational Biochemistry Group
PDF File - Computational Biochemistry Group

... Such thiyl radicals usually activate a substrate molecule by hydrogen atom abstraction, although besides addition to a carbonyl group, oxidation of an anion or other chemical transformation can occur [Frey et al., 2008]. The catalytic pathway then proceeds via intermediate radicals until the product ...
Fate of ammonium15N in a Norway spruce forest under long
Fate of ammonium15N in a Norway spruce forest under long

... to NO by V(III)Cl3 in HCl. As also NO2- was reduced to NO by V(III)Cl3, the formed NO represents a mixture of both N-species. In soil solutions NO3predominates over NO2-, hence we did not correct for NO2-. 15N abundances were expressed as atom%. Microbial biomass Microbial biomass N was determined b ...
Characterization of the Plasmodium falciparum and P. berghei
Characterization of the Plasmodium falciparum and P. berghei

... studies with the human malaria parasite Plasmodium falciparum and the rodent models Plasmodium berghei and Plasmodium yoelii subsequently overturned this assumption, with FASII enzymes found to be minimally expressed (Bozdech et al., 2003; Le Roch et al., 2003; Llinás, 2006) and ultimately dispensab ...
video slide
video slide

... molecules and yields ATP • Although carbohydrates, fats, and proteins are all consumed as fuel, it is helpful to trace cellular respiration with the sugar glucose: C6H12O6 + 6O2  6CO2 + 6H2O + Energy (ATP + heat) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
2 H
2 H

... molecules and yields ATP • Although carbohydrates, fats, and proteins are all consumed as fuel, it is helpful to trace cellular respiration with the sugar glucose: C6H12O6 + 6O2  6CO2 + 6H2O + Energy (ATP + heat) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
The change from lipid to carbohydrate during the respiratory rise in
The change from lipid to carbohydrate during the respiratory rise in

... kind, and it is a matter of some interest to identify the actual endogenous substrate. Respiration in plant tissues may comprise more than one respiratory path. The matter is further complicated by evidence that the relative contribution of coexisting respiratory paths may change with time, as is th ...
Arginine-induced stimulation of protein synthesis and survival in
Arginine-induced stimulation of protein synthesis and survival in

... to synthesize arginine (20, 37, 41), and the small intestine is a key site of net arginine synthesis in neonates (39, 42, 43). Thus the gut appears to play a critical role in maintaining arginine homeostasis in neonates (13, 40). Evidence from in vitro metabolic studies with primary cells and transf ...
THE APPLICATION OF TANDEM MASS SPECTROMETRY TO
THE APPLICATION OF TANDEM MASS SPECTROMETRY TO

... important to note that quantification of the compounds in a DBS is different from clinical analyses that use liquid blood, plasma, or urine samples. Traditional isotope dilution techniques require addition of a stable isotope standard to a liquid blood sample, where it can uniformly mix with the unl ...
Molecular Characterisation of the 76 kDa Iron
Molecular Characterisation of the 76 kDa Iron

... between the mitochondrial and nuclear genomes in different organisms, whereas genes for a homologous multisubunit complex in chloroplasts have to date only been found on the plastid genome. In potato (Solatium tuberosum L.), the gene coding for the mitochondrial 76 kDa iron-sulphur protein is identi ...
2.2. Garrido-Franco, M. Structure E. coli
2.2. Garrido-Franco, M. Structure E. coli

... remarkable difference is the binding of a phosphate ion in the active site of monomer A. The average B factor of the Pi (54.1 Å2) is slightly higher than the B factors of the surrounding residues (45.6 Å2) and the PNP (33.5 Å2). Attempts to refine a Pi at an equivalent position in monomer B, C and D ...
metabolic pathways - MPG Systems Biology Forum
metabolic pathways - MPG Systems Biology Forum

... D. Letscher and B.O. Palsson, J. theor. Biol. 203 (2000) 229) - distinction between internal and exchange reactions, all internal reversible reactions are split up into forward and reverse steps ...
2 H + 1 / 2 O 2
2 H + 1 / 2 O 2

... • The citric acid cycle, also called the Krebs cycle, takes place within the mitochondrial matrix • The cycle oxidizes organic fuel derived from pyruvate, generating 1 ATP, 3 NADH, and 1 FADH2 per turn ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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