1 Old Exam I Questions Choose an answer of A,B, C, or D for each
... A) Ionic bonds on the external surface of the folded protein are more stable than those buried in the protein interior. B) In an ionic bond, a hydrogen atom carrying a partial positive charge on the electronegative atom of one covalent bond interacts with the partial negative charge of an electroneg ...
... A) Ionic bonds on the external surface of the folded protein are more stable than those buried in the protein interior. B) In an ionic bond, a hydrogen atom carrying a partial positive charge on the electronegative atom of one covalent bond interacts with the partial negative charge of an electroneg ...
... courses of pNP formation from reactions of 21B3 on 12pNCA in 1 mm, 5 mm, and 10 mm H2O2. The enzyme is essentially inactive within 5 minutes in 10 mm H2O2. Inactivation appears to be turnover-dependent (similar TONs are reached from reactions in 1 mm, 5 mm, and 10 mm H2O2), and the ratio of peroxyge ...
Archaea
... • Consists of many classes, orders, and families • Often divided informally into five major groups 1. methanogens 2. halobacteria 3. thermoplasms 4. extremely thermophilic S0-metabolizers 5. sulfate-reducers ...
... • Consists of many classes, orders, and families • Often divided informally into five major groups 1. methanogens 2. halobacteria 3. thermoplasms 4. extremely thermophilic S0-metabolizers 5. sulfate-reducers ...
Deficient Saccharomyces cerevisiae and on the
... Initially a survey was made of the biotin-sparing activity of a number of substances (including amino acids, purines and related compounds, fatty acids) the hiosyntheses of which are known to be biotin-dependent. The growth-promoting action of each of thcsc substances, with the exception of oleic ac ...
... Initially a survey was made of the biotin-sparing activity of a number of substances (including amino acids, purines and related compounds, fatty acids) the hiosyntheses of which are known to be biotin-dependent. The growth-promoting action of each of thcsc substances, with the exception of oleic ac ...
Powerpoint on Proteins
... pointing toward the C-terminus. Adjacent β strands can form hydrogen bonds in antiparallel, parallel, or mixed arrangements. Antiparallel β strands alternate directions so that the N-terminus of one strand is adjacent to the Cterminus of the next. This produces the strongest inter-strand stability b ...
... pointing toward the C-terminus. Adjacent β strands can form hydrogen bonds in antiparallel, parallel, or mixed arrangements. Antiparallel β strands alternate directions so that the N-terminus of one strand is adjacent to the Cterminus of the next. This produces the strongest inter-strand stability b ...
File
... The TCA cycle occurs twice for every molecule of glucose oxidized The net result is 2 ATP and 4 CO2 The overall reaction for glycolysis, acetyl CoA formation and TCA cycle ...
... The TCA cycle occurs twice for every molecule of glucose oxidized The net result is 2 ATP and 4 CO2 The overall reaction for glycolysis, acetyl CoA formation and TCA cycle ...
Natural abundance of 15N in amino acids and
... the possibility that reactions to form homospd in nodule bacteroids may include the processes resulting in higher 15N enrichment in homospd than in rhizobia growing in the synthetic liquid media. Both put in rhizobia and in bacteroids may be synthesized via the ornithine pathway and/or from arginine ...
... the possibility that reactions to form homospd in nodule bacteroids may include the processes resulting in higher 15N enrichment in homospd than in rhizobia growing in the synthetic liquid media. Both put in rhizobia and in bacteroids may be synthesized via the ornithine pathway and/or from arginine ...
Clues from cell metabolism
... of the enzyme dihydrofolate reductase. This NADPH-dependent enzyme has a central role in nucleic-acid synthesis, and is activated by the high redox state induced by aerobic glycolysis and/or glutamine breakdown. Methotrexate, a related aminopterin compound, remains an important anticancer drug. Othe ...
... of the enzyme dihydrofolate reductase. This NADPH-dependent enzyme has a central role in nucleic-acid synthesis, and is activated by the high redox state induced by aerobic glycolysis and/or glutamine breakdown. Methotrexate, a related aminopterin compound, remains an important anticancer drug. Othe ...
the enzymatic synthesis of protein. i.
... important observation that neither enzyme was capable of producing plastein from any type of digest of gelatin; nor from any tryptic digest except of casein, and here only by pepsin. There is indicated in their work the possibility of the influence of dilution, of temperature, and of acidity on the ...
... important observation that neither enzyme was capable of producing plastein from any type of digest of gelatin; nor from any tryptic digest except of casein, and here only by pepsin. There is indicated in their work the possibility of the influence of dilution, of temperature, and of acidity on the ...
Biochemistry I, Spring Term 2005 - Second Exam:
... d) All of the above are correct. 2. In both hemoglobin and myoglobin the oxygen is bound to. a) the nitrogen atoms on the heme. b) polar pocket in the protein. c) histidine residues in the protein. d) the iron atom in the heme group. 3. A protein that binds two ligands in a non-cooperative manner wi ...
... d) All of the above are correct. 2. In both hemoglobin and myoglobin the oxygen is bound to. a) the nitrogen atoms on the heme. b) polar pocket in the protein. c) histidine residues in the protein. d) the iron atom in the heme group. 3. A protein that binds two ligands in a non-cooperative manner wi ...
Biochemistry for the Radiation Biologist
... donor; one CO2 molecule gets lost for each cycle Requires a lot of ATP equivalents for energy The cycle involves five enzymes, each of which must contribute to each 2-carbon growth Builds up to C16 or C18; then enzymes release products But until then the reactions are very tightly coupled because th ...
... donor; one CO2 molecule gets lost for each cycle Requires a lot of ATP equivalents for energy The cycle involves five enzymes, each of which must contribute to each 2-carbon growth Builds up to C16 or C18; then enzymes release products But until then the reactions are very tightly coupled because th ...
Feb20
... Discussion of PAM • The 1 PAM matrix was derived by constructing hypothetical phylogenetic trees relating sequences in 71 families. • The higher the power of the matrix, the more evolutionary time units represented by the matrix. • Criticism – raising M to high powers does not capture the true diff ...
... Discussion of PAM • The 1 PAM matrix was derived by constructing hypothetical phylogenetic trees relating sequences in 71 families. • The higher the power of the matrix, the more evolutionary time units represented by the matrix. • Criticism – raising M to high powers does not capture the true diff ...
Molecular genetics of nucleotide sugar interconversion pathways in
... mur1-8; mur stands for Latin murus, the wall) that are defective in the de novo synthesis of L-fucose were isolated as part of a larger effort to isolate cell wall mutants by directly screening for changes in the monosaccharide composition of leaf cell wall material (Reiter et al., 1993, 1997). Muta ...
... mur1-8; mur stands for Latin murus, the wall) that are defective in the de novo synthesis of L-fucose were isolated as part of a larger effort to isolate cell wall mutants by directly screening for changes in the monosaccharide composition of leaf cell wall material (Reiter et al., 1993, 1997). Muta ...
Biochemistry 2000 Sample Questions 5 Transport, Carbohydrates, Metabolism
... (b) A ketohexose in the linear and cyclic, α-anomeric form (c) A disaccharide of two riboses connected by a (β1-α1) glycosidic bond. (d) A disaccharide building block of the polysaccharide amylose. (5) The transformed, standard free energy change for the formation of glucose-6phosphate from glucose ...
... (b) A ketohexose in the linear and cyclic, α-anomeric form (c) A disaccharide of two riboses connected by a (β1-α1) glycosidic bond. (d) A disaccharide building block of the polysaccharide amylose. (5) The transformed, standard free energy change for the formation of glucose-6phosphate from glucose ...
A tale of two functions: enzymatic activity and
... regions of both subunit mRNAs and inhibits translation, an inhibition that is relieved by the substrate acetyl-CoA. mRNA binding reciprocally inhibits catalytic activity. Preferential binding of carboxyltransferase to RNA in situ was shown using fluorescence resonance energy transfer. We propose an ...
... regions of both subunit mRNAs and inhibits translation, an inhibition that is relieved by the substrate acetyl-CoA. mRNA binding reciprocally inhibits catalytic activity. Preferential binding of carboxyltransferase to RNA in situ was shown using fluorescence resonance energy transfer. We propose an ...
Chem 465 Biochemistry II
... Pyruvate dehydrogenase and á-ketoglutarate dehydrogenase are very similar in structure and mechanism. Both are large enzyme complexes that contain multiple copies of three major proteins usually called simply E1, E2, and E3. Both enzyme complexes also utilize the cofactors thiamine pyrophosphate, li ...
... Pyruvate dehydrogenase and á-ketoglutarate dehydrogenase are very similar in structure and mechanism. Both are large enzyme complexes that contain multiple copies of three major proteins usually called simply E1, E2, and E3. Both enzyme complexes also utilize the cofactors thiamine pyrophosphate, li ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.