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Biochemistry I, Spring Term 2003 - Second Exam:
Biochemistry I, Spring Term 2003 - Second Exam:

... the protein and the ligand. You need not calculate ∆Go, a qualitative discussion will suffice. Hint: ∆Go = ∆Ho T∆So. (4 pts) ...
NOW Foods - 5-HTP 50 mg 5-Hydroxy-L-Tryptophan
NOW Foods - 5-HTP 50 mg 5-Hydroxy-L-Tryptophan

... 5-HTP (5-hydroxytryptophan) is a metabolite of the amino acid tryptophan. You may know tryptophan as the agent in turkey that makes you feel like taking a nap after Thanksgiving dinner (in addition to a full belly). 5-HTP is a direct precursor of the important inhibitory neurotransmitter serotonin, ...
Supplementary document Trehalose/2
Supplementary document Trehalose/2

... of the NhaC family, and a multisubunit Na+:H+ antiporter similar to MnhABCDEFG. As ...
NOW Foods - 5-HTP 50 mg 5-Hydroxy-L-Tryptophan
NOW Foods - 5-HTP 50 mg 5-Hydroxy-L-Tryptophan

... 5-HTP (5-hydroxytryptophan) is a metabolite of the amino acid tryptophan. You may know tryptophan as the agent in turkey that makes you feel like taking a nap after Thanksgiving dinner (in addition to a full belly). 5-HTP is a direct precursor of the important inhibitory neurotransmitter serotonin, ...
Xu-7-integration
Xu-7-integration

... Blood glucose levels are not constant—they rise and fall depending on the body's needs, regulated by hormones. This results in glucose levels normally ranging from 70 to 110 mg/dl. The blood glucose level can rise for three reasons: diet, breakdown of glycogen, or through hepatic synthesis of glucos ...
elisa - WordPress.com
elisa - WordPress.com

Biochemistry I, Spring Term 2004 - Second Exam:
Biochemistry I, Spring Term 2004 - Second Exam:

... d) the dissociation constant cannot be determined from a binding curve in this case. 4. The oxygen bound to hemoglobin or myoglobin is directly attached to the a) helix-F in the protein. b) the proximal histidine. c) the iron atom. d) the heme group. 5. Enzymes increase the rate of chemical reaction ...
Biochemistry I, Spring Term 2004 - Second Exam:
Biochemistry I, Spring Term 2004 - Second Exam:

... d) the dissociation constant cannot be determined from a binding curve in this case. 4. The oxygen bound to hemoglobin or myoglobin is directly attached to the a) helix-F in the protein. b) the proximal histidine. c) the iron atom. d) the heme group. 5. Enzymes increase the rate of chemical reaction ...
chromatography
chromatography

... unknown amino acids is seen to contain four different amino acids Of these four amino acids, two can be positively identified The mixture contains four amino acids; two unknown together with arginine & leucine ...
Bacterial-Invertebrate Interactions in Uptake of Dissolved Organic
Bacterial-Invertebrate Interactions in Uptake of Dissolved Organic

... Since bacteria rely exclusively on dissolved nutrients, microbial degradation of particulate detritus introduced by meta" i zoans includes secretion of extracellular enzymes and hydrolysis of solid substrates to yield the low molecular weight organic FIG. 5. Nereis diversicolor, attracted by small p ...
The Effect of Actidione and other Antifungal Agents on Nucleic Acid
The Effect of Actidione and other Antifungal Agents on Nucleic Acid

... there was complete inhibition of DNA synthesis whereas the synthesis of RNA was not completely inhibited at 100 pg. actidione/ml. (Fig. 2). The residual RNA synthesis in the presence of actidione could account for the degree of inhibition observed when total nucleic acid synthesis was measured, sinc ...
Tricarboxylic Acid Cycle (TCA), Krebs Cycle
Tricarboxylic Acid Cycle (TCA), Krebs Cycle

... Regulation of Pyr. Dehydrogenase Complex Allosteric activation of kinase & Phosphatase: - Cyclic AMP-independent protein kinase ( activated)activates phosphorylated E1 ( inactive ) & inhibits dephosphorylated ( active )  inhibit Pyr DH. protein kinase allosterically activated by ATP, acetyl CoA, ...
Microbiology pathways
Microbiology pathways

... oxidation and reduction Electrons are passed down the chain in a sequential and orderly fashion Energy is released from the flow of electrons down the chain This release of energy is coupled to the generation ATP by oxidative phosphorylation ...
A report on TAK-875 analysis using the Heptox Virtual Liver Platform
A report on TAK-875 analysis using the Heptox Virtual Liver Platform

... Perturbation in mitochondrial ETC causes depletion of cellular ATP due to depolarisation of mitochondrial membrane resulting in potential for necrotic cell death at higher exposure Minor change in cellular GSH is predicted Tak-875 is predicted to be a direct inhibitor of fatty acid transport into th ...
Cellular Metabolism Pathways
Cellular Metabolism Pathways

Supplementary Notes - Word file
Supplementary Notes - Word file

... that emit green (PplGR) and yellow-green light (PplYG) possess an Ile residue at the position corresponding to Ile288 of Lcr luciferase, but this position is substituted with a Val residue in the Ppl luciferases that emit light with longer wavelengths: yellow (PplYE) and orange (PplOR) (Fig. S2). We ...
Full Text
Full Text

... aligned protein sequences using standard statistical tests and for representing them with Bayesian networks. In this paper, we demonstrate the power of our discovery program and representation by analyzing pairs of residues from α-helices. The sequence correlations we find represent physical and che ...
Sathgudi Sweet orange
Sathgudi Sweet orange

... for 5 minutes. iii. The tube was heated in a 42oC in water bath or heating block for 30 seconds without shaking.iv. The tube was then incubated on ice for 2 minutes. v. 250 µl SOC medium was added to the tube and 100 µl of each transformation mixture was then directly plated on to Luria ...
Cellular Respirationx
Cellular Respirationx

... groups are added to the glucose molecule from 2 ATPs, making a 6-C compound that can be split. Step 2 – The 6-C compound is split into two 3-C molecules of PGAL (phosphoglyceraldehyde). Step 3 – The two PGAL molecules are oxidized and receive another phosphate group. The oxidation of PGAL accompanie ...
ScrFl restriction/modification system from
ScrFl restriction/modification system from

... activity. Overnight cultures of E. coli and L. lactis were pelleted by centrifugation, washed in quarter-strength Ringer's solution and resuspended in 0.75 ml ice-cold extraction buffer : 0.05 M Tris/HCl p H 7.5, 1 mM Na,EDTA and 7 mM 2mercaptoethanol. Cells were ruptured by adding 0.5 g glass beads ...
physiology – metabolism
physiology – metabolism

... E. All of the above 14. Excretion of the following substance does NOT reduce in starvation: A. Urea B. Uric acid C. Creatinine D. Etheral sulfate E. All of the above 15. Prolonged starvation in non-diabetic man is NOT characterized by: A. Ketosis B. Hypoglycemia C. Glycogen depletion D. Protein cata ...
Document
Document

... forming a transient covalent bond with residues in the active site of the enzyme or with a cofactor. • This adds an additional covalent intermediate to the reaction, and helps to reduce the energy of later transition states of the reaction. • Group X can be transferred from A-X to B in two steps via ...
Learning objectives
Learning objectives

medical chemistry and biochemistry
medical chemistry and biochemistry

Dining Out—The Pollinator Buffet
Dining Out—The Pollinator Buffet

... Dining Out—The Pollinator Buffet Not all flowers can provide pollinators with the food and nutrition they need. Also, not all pollinators are able to eat at all flowers due to their different body parts. So, pollinators are picky eaters! One thing most pollinators do have in common is their nutritio ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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