Molecular evolution of the p53 network in reptiles
... apoptosis, cellular senescence, and temporary arrest of cellular growth for DNA repair. The p53 network’s tight regulation of cellular fate after damage has obvious beneficial effects of preventing tumorigenesis, and possible costly effects later in life such as the accumulat ...
... apoptosis, cellular senescence, and temporary arrest of cellular growth for DNA repair. The p53 network’s tight regulation of cellular fate after damage has obvious beneficial effects of preventing tumorigenesis, and possible costly effects later in life such as the accumulat ...
Discovery of a novel ligand that modulates the
... Fig. 1 Identification of small molecules targeting reptin using an in silico screening programme. (a) Structure of hit compound 1 was identified through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtain ...
... Fig. 1 Identification of small molecules targeting reptin using an in silico screening programme. (a) Structure of hit compound 1 was identified through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtain ...
The ~53 Proto-Oncogene Can Act as a Suppressor of Transformation
... et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been detected in osteosarcomas (Friend et al., 1986) small ...
... et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been detected in osteosarcomas (Friend et al., 1986) small ...
Winter 2011 - Society of Toxicology
... tumors relative to Elston Grade 1 tumors at the p<0.0001 level of significance. Our results suggest that decreased FRY expression or loss is associated with the least differentiated and most aggressive grade of tumor types. These studies support the hypothesis that the mammalian FRY gene encodes a t ...
... tumors relative to Elston Grade 1 tumors at the p<0.0001 level of significance. Our results suggest that decreased FRY expression or loss is associated with the least differentiated and most aggressive grade of tumor types. These studies support the hypothesis that the mammalian FRY gene encodes a t ...
Update on the Pathogenesis and Immunotherapy of Esophageal
... Assessment of p53 mutations status may be clinically important as a parameter for the definition of risk groups after potentiallycurativeresections(34). ...
... Assessment of p53 mutations status may be clinically important as a parameter for the definition of risk groups after potentiallycurativeresections(34). ...
E-selectin prefers fatty-sweet receptors on rolling neutrophils
... UNIVERSITY OF CALIFORNIA AT DAVIS ...
... UNIVERSITY OF CALIFORNIA AT DAVIS ...
New drugs for hepatitis C
... Nakamoto, S., Kanda, T., Wu, S., Shirasawa, H., & Yokosuka, O. (2014). Hepatitis C virus NS5A inhibitors and drug resistance mutations. World Journal of Gastroenterology : WJG, 20(11), 2902–2912. ...
... Nakamoto, S., Kanda, T., Wu, S., Shirasawa, H., & Yokosuka, O. (2014). Hepatitis C virus NS5A inhibitors and drug resistance mutations. World Journal of Gastroenterology : WJG, 20(11), 2902–2912. ...
Interactions between the otitis media gene, Fbxo11, and p53 in the
... Our previous studies of the Jeff mutant indicate that FBXO11 is involved in controlling TGFβ signalling by regulating the levels of pSMAD2 in embryonic epithelial cells (Tateossian et al., 2009). While Jeff heterozygous mice develop deafness due to chronic proliferative otitis media (Hardisty et al ...
... Our previous studies of the Jeff mutant indicate that FBXO11 is involved in controlling TGFβ signalling by regulating the levels of pSMAD2 in embryonic epithelial cells (Tateossian et al., 2009). While Jeff heterozygous mice develop deafness due to chronic proliferative otitis media (Hardisty et al ...
- Wiley Online Library
... and p53 is rapidly stabilized [3]. The accumulated p53 protein is subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination, sumoylation, neddylation and glycosylation (reviewed in references [4,5]). It is broadly accepted that these mo ...
... and p53 is rapidly stabilized [3]. The accumulated p53 protein is subject to extensive post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination, sumoylation, neddylation and glycosylation (reviewed in references [4,5]). It is broadly accepted that these mo ...
p53, the Cellular Gatekeeper Review for Growth and Division
... Product of an oncogene; inactivates p53-mediated transcription and so forms an autoregulatory loop with p53 activity GADD45 Induced upon DNA damage; binds to PCNA and can arrest the cell cycle; involved directly in DNA nucleotide excision repair Cyclin G A novel cyclin (it does not cycle with cell d ...
... Product of an oncogene; inactivates p53-mediated transcription and so forms an autoregulatory loop with p53 activity GADD45 Induced upon DNA damage; binds to PCNA and can arrest the cell cycle; involved directly in DNA nucleotide excision repair Cyclin G A novel cyclin (it does not cycle with cell d ...
Simian Virus 40 Large T Antigen and p53 Are
... Lanes 1 and 2 with Lanes 3-10). Each of the M, 45,00060,000 proteins ...
... Lanes 1 and 2 with Lanes 3-10). Each of the M, 45,00060,000 proteins ...
msb20105-sup-0002 - Molecular Systems Biology
... As in our previous model (Proctor & Gray, 2008), we assume that DNA damage activates ATM which then phosphorylates both p53 and Mdm2 to interrupt their binding. This leads to stabilisation of p53. Since p53 is a transcription factor for Mdm2, there is also an increase in Mdm2 levels, which then bind ...
... As in our previous model (Proctor & Gray, 2008), we assume that DNA damage activates ATM which then phosphorylates both p53 and Mdm2 to interrupt their binding. This leads to stabilisation of p53. Since p53 is a transcription factor for Mdm2, there is also an increase in Mdm2 levels, which then bind ...
emboj7601343-sup
... analyzed for cell cycle distribution with a FACS Calibur flow cytometer (fluorescenceactivated cell sorter; FACS) and CellQuest software (Becton Dickinson). Red fluorescence (585 ± 42 nm) was evaluated on a linear scale, and pulse width analysis was used to exclude cell doublets and aggregates from ...
... analyzed for cell cycle distribution with a FACS Calibur flow cytometer (fluorescenceactivated cell sorter; FACS) and CellQuest software (Becton Dickinson). Red fluorescence (585 ± 42 nm) was evaluated on a linear scale, and pulse width analysis was used to exclude cell doublets and aggregates from ...
p53 Pathway in Renal Cell Carcinoma Is Repressed by a Dominant
... HeLa (cervical carcinoma), A293 (embryonic kidney epithelial cells transRenal cell carcinoma (RCC) accounts for approximately 85% of all formed with DNA of adenovirus type 5), and LNCaP (prostate adenocarcikidney cancers. Approximately 30,000 patients in the United States noma)] were purchased from ...
... HeLa (cervical carcinoma), A293 (embryonic kidney epithelial cells transRenal cell carcinoma (RCC) accounts for approximately 85% of all formed with DNA of adenovirus type 5), and LNCaP (prostate adenocarcikidney cancers. Approximately 30,000 patients in the United States noma)] were purchased from ...
Phosphatases - Georgia Institute of Technology
... • adrenaline β-AR AC PKA – PKAPhK phosphorylaseglycogen breakdown into glucose • PhK--|GSsynthesis of glycogen from glucose ...
... • adrenaline β-AR AC PKA – PKAPhK phosphorylaseglycogen breakdown into glucose • PhK--|GSsynthesis of glycogen from glucose ...
p53 transcriptional activity is essential for p53dependent apoptosis
... p53 is the most commonly mutated tumor suppressor gene in human cancers, and its role in tumor suppression is further highlighted by the creation of p53±/± mice, which are highly cancer prone and develop a large spectrum of tumors (Donehower et al., 1992; Jacks et al., 1994). It has become clear tha ...
... p53 is the most commonly mutated tumor suppressor gene in human cancers, and its role in tumor suppression is further highlighted by the creation of p53±/± mice, which are highly cancer prone and develop a large spectrum of tumors (Donehower et al., 1992; Jacks et al., 1994). It has become clear tha ...
Prediction of protein disorder: basic concepts and practical hints
... Disordered protein complexes • Interaction sites are usually linear ...
... Disordered protein complexes • Interaction sites are usually linear ...
Gene Section NLRC4 (NLR Family, CARD domain containing 4)
... NLRC4 as truncation of this domain makes the protein constitutively active. NLRC4 is involved in the regulation of caspase-1, which is activated within the 'inflammasome', a complex comprising several adaptors and permitting pro-IL-1beta processing and secretion of mature IL1beta. It is required for ...
... NLRC4 as truncation of this domain makes the protein constitutively active. NLRC4 is involved in the regulation of caspase-1, which is activated within the 'inflammasome', a complex comprising several adaptors and permitting pro-IL-1beta processing and secretion of mature IL1beta. It is required for ...
TP53 Mutations In Human Skin Cancers
... may give rise to mutations, after one or two rounds of replication, all along the genomic DNA including tumor suppressor genes (such as the p53 gene TP53; MIM# 191170) or proto-oncogenes (such as the ras family genes) and therefore can lead to carcinogenesis [Dumaz et al., 1994]. The general dogma i ...
... may give rise to mutations, after one or two rounds of replication, all along the genomic DNA including tumor suppressor genes (such as the p53 gene TP53; MIM# 191170) or proto-oncogenes (such as the ras family genes) and therefore can lead to carcinogenesis [Dumaz et al., 1994]. The general dogma i ...
Regulation of developmental timing and germ cell proliferation in C
... The p53 tumor suppressor regulates a large number of genes that collaborate to modulate cell proliferation, DNA repair, apoptosis, and senescence; however, little is known about how this complex response is orchestrated to dictate various cellular outcomes in response to cellular stresses.1 Activati ...
... The p53 tumor suppressor regulates a large number of genes that collaborate to modulate cell proliferation, DNA repair, apoptosis, and senescence; however, little is known about how this complex response is orchestrated to dictate various cellular outcomes in response to cellular stresses.1 Activati ...
Novel p53 mutants selected in BRCA
... inducer of reactive oxygen species (ROS) which are powerful inducers of apoptosis. The importance of transactivation in mediating the biological eects of p53 has been illustrated by studies of human tumour associated p53 mutations the vast majority of which occur in evolutionarily conserved regions ...
... inducer of reactive oxygen species (ROS) which are powerful inducers of apoptosis. The importance of transactivation in mediating the biological eects of p53 has been illustrated by studies of human tumour associated p53 mutations the vast majority of which occur in evolutionarily conserved regions ...
New insights into regulation of p53 protein degradation
... E4orf6, E1B55K and BZLF1 [21], these Mdm2independent ubiquitination proteasome pathways also are involved in p53 degradation. Covalent modifications of p53, include phosphorylation, acetylation, methylation, ubiquitination, sumoylation, neddylation, glycosylation, ribosylation and O-GlcNAcylation [2 ...
... E4orf6, E1B55K and BZLF1 [21], these Mdm2independent ubiquitination proteasome pathways also are involved in p53 degradation. Covalent modifications of p53, include phosphorylation, acetylation, methylation, ubiquitination, sumoylation, neddylation, glycosylation, ribosylation and O-GlcNAcylation [2 ...
Expression of the papillomavirus E2 protein in HeLa cells leads to
... genotoxic agents (Butz et al., 1995). Since E2 could repress E6 expression, we next investigated whether this resulted in stabilization of p53. The activity of p53 was assayed via co-transfected p53-responsive CAT reporter plasmids. As shown in Figure 2A, expression of fulllength BPV-1 E2 resulted i ...
... genotoxic agents (Butz et al., 1995). Since E2 could repress E6 expression, we next investigated whether this resulted in stabilization of p53. The activity of p53 was assayed via co-transfected p53-responsive CAT reporter plasmids. As shown in Figure 2A, expression of fulllength BPV-1 E2 resulted i ...
Nonhereditary p53 Mutations in T-cell Acute
... of which had been grownfrom relapse T-ALL cases, possess mutations on both p53 suggesting that p53 serves a critical role in the generation of the fully tumorigenic leukemic T cells. However, our experiments did not show whether these p53 mutations originated in vivo or whether the p53 gene had been ...
... of which had been grownfrom relapse T-ALL cases, possess mutations on both p53 suggesting that p53 serves a critical role in the generation of the fully tumorigenic leukemic T cells. However, our experiments did not show whether these p53 mutations originated in vivo or whether the p53 gene had been ...
Supplemental Digital Content
... The stainings were performed on the Autostainer link 48 (AS480, DAKO, Glostrup, Denmark). Here the slides were submitted to blocking of endogen peroxidase and incubated with the primary antibody for 30 minutes. A Mouse LINKER was then added for 20 minutes to enhance the signal and after washing in w ...
... The stainings were performed on the Autostainer link 48 (AS480, DAKO, Glostrup, Denmark). Here the slides were submitted to blocking of endogen peroxidase and incubated with the primary antibody for 30 minutes. A Mouse LINKER was then added for 20 minutes to enhance the signal and after washing in w ...
P53
Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. As such, p53 has been described as ""the guardian of the genome"" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene.The name p53 was given in 1979 describing the apparent molecular mass; SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. In addition to the full length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to 53 kDa. All these p53 proteins are called the p53 isoforms.The International Cancer Genome Consortium has established that the TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation.TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome.