Sp100 is important for the stimulatory effect of
... expression. Coexpression of HIPK2 and Sp100 synergistically stimulated reporter gene activity, thus suggesting a transactivating role for Sp100. To ensure that the observed effects are dependent on the p53-binding sites, the experiment was repeated using the pMG15-luciferase reporter gene containing ...
... expression. Coexpression of HIPK2 and Sp100 synergistically stimulated reporter gene activity, thus suggesting a transactivating role for Sp100. To ensure that the observed effects are dependent on the p53-binding sites, the experiment was repeated using the pMG15-luciferase reporter gene containing ...
Types of Programmed Cell Death The mechanisms by which cells
... aging and contribute to aging-related loss of function in various adult tissues. This accumulation may result from the fact that senescent cells are resistant to apoptosis due to repressed activity of PCD pathway components such as caspase 3 and cell cycle factors that function in both cell division ...
... aging and contribute to aging-related loss of function in various adult tissues. This accumulation may result from the fact that senescent cells are resistant to apoptosis due to repressed activity of PCD pathway components such as caspase 3 and cell cycle factors that function in both cell division ...
Quantification of p53-expressing cells and neurodegenerative cells
... Exposure to PBDEs during the brains critical growth period has shown to induce permanent developmental neurotoxicity in adult mice. This has not been observed with TBBPA. The mechanism for how the neurotoxicity is induced is still unclear, however it is suggested that apoptosis play a major role. Ap ...
... Exposure to PBDEs during the brains critical growth period has shown to induce permanent developmental neurotoxicity in adult mice. This has not been observed with TBBPA. The mechanism for how the neurotoxicity is induced is still unclear, however it is suggested that apoptosis play a major role. Ap ...
Male idiopathic infertility and the TP53 polymorphism in
... cells (Jobling and Tyler-Smith, 2003). Maintaining the integrity of testicular germ cells during spermatogenesis is crucial to fertility. Spontaneous germ cell death has been recognized as a normal cellular process in the mammalian testis (Kerr, 1992). Cell suppression has been found at different st ...
... cells (Jobling and Tyler-Smith, 2003). Maintaining the integrity of testicular germ cells during spermatogenesis is crucial to fertility. Spontaneous germ cell death has been recognized as a normal cellular process in the mammalian testis (Kerr, 1992). Cell suppression has been found at different st ...
Supplementary Information (doc 30K)
... Fig. S1. Western blot analysis in HEp2 for stable USP7 depletion and HEp2 or H1299 cells to compare expression of p53, Daxx and USP7. (A) Western blot representing USP7 depletion in HEp2 cells stably depleted by control or USP7 shRNAs. (B) Western blot analysis of p53, Daxx and USP7 in HEp2 and H129 ...
... Fig. S1. Western blot analysis in HEp2 for stable USP7 depletion and HEp2 or H1299 cells to compare expression of p53, Daxx and USP7. (A) Western blot representing USP7 depletion in HEp2 cells stably depleted by control or USP7 shRNAs. (B) Western blot analysis of p53, Daxx and USP7 in HEp2 and H129 ...
Deletions of 17p and p53 Mutations in
... cancer, and of growth factor receptor genes in non-small cell lung cancer (1—3).The involvement of known or presumed tumor suppressor genes has been suggested by the presence of recurrent chromosomal deletions or losses and confirmed by restriction fragment length polymorphism analyses showing los ...
... cancer, and of growth factor receptor genes in non-small cell lung cancer (1—3).The involvement of known or presumed tumor suppressor genes has been suggested by the presence of recurrent chromosomal deletions or losses and confirmed by restriction fragment length polymorphism analyses showing los ...
p53 AND CANCER - Journal of Chemical and Pharmaceutical
... Trp53 is a master transcription factor which regulates the expression of a plethora of genes involved in the crucial biological processes, many of which encipher proteins that control the cell cycle or induce apoptosis. Because of its critical impact on cell predestination, cellular p53 activity mus ...
... Trp53 is a master transcription factor which regulates the expression of a plethora of genes involved in the crucial biological processes, many of which encipher proteins that control the cell cycle or induce apoptosis. Because of its critical impact on cell predestination, cellular p53 activity mus ...
HAUSP, a deubiquitinating enzyme for p53, is polyubiquitinated
... Western blotting; WCL, whole cell lysate ...
... Western blotting; WCL, whole cell lysate ...
PDF
... processes in the developing Drosophila CNS. They show that canonical Notch signalling in specialised glial cells causes the extrusion of a mesh of fine filopodia by nearby differentiating neurons and shapes this mesh into a carpet that links adjacent segments. Simultaneously, non-canonical Notch sig ...
... processes in the developing Drosophila CNS. They show that canonical Notch signalling in specialised glial cells causes the extrusion of a mesh of fine filopodia by nearby differentiating neurons and shapes this mesh into a carpet that links adjacent segments. Simultaneously, non-canonical Notch sig ...
Cancer What is cancer? Properties of cancer cells Properties of
... be activated by various pathways: phosphorylation (Hupp et al., 1992), antibody specific for the carboxy-terminus of the protein (Hupp et al., 1992), small peptides which could mimic the carboxy-terminus of the p53 (Hupp et al., 1995), short single stranded DNA (Jayaraman & Prives, 1995), deletion o ...
... be activated by various pathways: phosphorylation (Hupp et al., 1992), antibody specific for the carboxy-terminus of the protein (Hupp et al., 1992), small peptides which could mimic the carboxy-terminus of the p53 (Hupp et al., 1995), short single stranded DNA (Jayaraman & Prives, 1995), deletion o ...
Cancer
... be activated by various pathways: phosphorylation (Hupp et al., 1992), antibody specific for the carboxy-terminus of the protein (Hupp et al., 1992), small peptides which could mimic the carboxy-terminus of the p53 (Hupp et al., 1995), short single stranded DNA (Jayaraman & Prives, 1995), deletion o ...
... be activated by various pathways: phosphorylation (Hupp et al., 1992), antibody specific for the carboxy-terminus of the protein (Hupp et al., 1992), small peptides which could mimic the carboxy-terminus of the p53 (Hupp et al., 1995), short single stranded DNA (Jayaraman & Prives, 1995), deletion o ...
Supplemental Methods
... according to which, residues that vary amongst closer homologous sequences are ranked less important than those that only vary amongst distant homologous sequences. The magnitude of the substitution ( i) was measured by ranks of amino acid substitution odds [3], however, these odds were computed fo ...
... according to which, residues that vary amongst closer homologous sequences are ranked less important than those that only vary amongst distant homologous sequences. The magnitude of the substitution ( i) was measured by ranks of amino acid substitution odds [3], however, these odds were computed fo ...
Supplemental Figure Legends
... Supplementary Figure Legends Supplementary Figure S1. Related to Figure 1. Unravelling the mechanism of C646 action. A–C, ChIP analysis was performed on MCF7 cells treated as in Fig. 1A, PCR products for the indicated genes were labeled with SYBR-green dye. Quantification was based on ΔCt values fro ...
... Supplementary Figure Legends Supplementary Figure S1. Related to Figure 1. Unravelling the mechanism of C646 action. A–C, ChIP analysis was performed on MCF7 cells treated as in Fig. 1A, PCR products for the indicated genes were labeled with SYBR-green dye. Quantification was based on ΔCt values fro ...
Inhibition of the p53 tumor suppressor by MDM2
... The P53 protein is classified as a tumor suppressor and is tasked with keeping the genomic stability of cells by inducing apoptosis and cell cycle arrest in cancerous cells (Frum, 2014). A key characteristic of p53 is its susceptibility to genetic mutation, these mutations can either be germline (pa ...
... The P53 protein is classified as a tumor suppressor and is tasked with keeping the genomic stability of cells by inducing apoptosis and cell cycle arrest in cancerous cells (Frum, 2014). A key characteristic of p53 is its susceptibility to genetic mutation, these mutations can either be germline (pa ...
ppt - Scientific Data Analysis Lab
... we have developed a neural network predictor (NNP) that uses amino acid sequence data to predict disorder in a given region. This Predictor of Natural Disordered Regions is termed PONDR®. ...
... we have developed a neural network predictor (NNP) that uses amino acid sequence data to predict disorder in a given region. This Predictor of Natural Disordered Regions is termed PONDR®. ...
Sarah Pulliam
... p53 gene in managing DNA repair. The Arf/p53 pathway is therefore known collectively as a tumor suppressor pathway (Blasco et al. 375). One of the main reasons the p53 gene is currently an area or intense research is due to its known cancer resistance effects. Scientists desire to determine the mec ...
... p53 gene in managing DNA repair. The Arf/p53 pathway is therefore known collectively as a tumor suppressor pathway (Blasco et al. 375). One of the main reasons the p53 gene is currently an area or intense research is due to its known cancer resistance effects. Scientists desire to determine the mec ...
Supplemental Figure legends
... extracted and analyzed by qRT-PCR for the induction of TRIML2 mRNA, relative to control (cyclophilin A). The gene expression level in 39˚C-incubated P72 cells was set to 1-fold and the data are normalized to cyclophilin A. The data depicted are the averaged results from three independent experiment ...
... extracted and analyzed by qRT-PCR for the induction of TRIML2 mRNA, relative to control (cyclophilin A). The gene expression level in 39˚C-incubated P72 cells was set to 1-fold and the data are normalized to cyclophilin A. The data depicted are the averaged results from three independent experiment ...
Immunohistochemistry Assessment of P53 Protein in Basal Cell
... The spectrum of p53 mutation in BCCs clearly shows that unrepaired photolesions resulting in UVinduced mutations play a decisive role in tumorigenesis. In the p53 gene, the CC to CT(TC) substitutions are strongly reminiscent of typical CC→TT transitions, specific to UVB-induced DNA damage.20 This is ...
... The spectrum of p53 mutation in BCCs clearly shows that unrepaired photolesions resulting in UVinduced mutations play a decisive role in tumorigenesis. In the p53 gene, the CC to CT(TC) substitutions are strongly reminiscent of typical CC→TT transitions, specific to UVB-induced DNA damage.20 This is ...
Gene Section TP53 (tumor protein p53 (Li-Fraumeni syndrome)) in Oncology and Haematology
... The five domains are highly-conserved regions between species (from human to fly). Two new genes homologous to p53 have been discovered, p73 localized at 1p36 and p63 localized at 3q27. ...
... The five domains are highly-conserved regions between species (from human to fly). Two new genes homologous to p53 have been discovered, p73 localized at 1p36 and p63 localized at 3q27. ...
p53 activates transcription of genes - California State University, Los
... Normal Cells are able to prevent cancer by activating a natural defense mechanism ...
... Normal Cells are able to prevent cancer by activating a natural defense mechanism ...
Chapter 12 Presentation-The Cell Cycle
... the “guardian of the genome”-it acts as a gate keeper. • The p53 gene makes p53 protein which does many different things. •It can arrest cell proliferation by holding the cell cycle at the G1/S checkpoint when DNA damage is p53 17q13.1: This gene encodes a tumor suppressor protein containing recogni ...
... the “guardian of the genome”-it acts as a gate keeper. • The p53 gene makes p53 protein which does many different things. •It can arrest cell proliferation by holding the cell cycle at the G1/S checkpoint when DNA damage is p53 17q13.1: This gene encodes a tumor suppressor protein containing recogni ...
Gene Section RPL26 (ribosomal protein L26) Atlas of Genetics and Cytogenetics
... within the 5' and 3'UTR of p53 mRNA creates a double-stranded RNA structure necessary for RPL26 binding to p53 mRNA. Disruption of this doublestranded RNA structure, either by base mutations in the two complementary UTR sequences or with a singlestranded oligonucleotide targeting the 5'-3'UTR base p ...
... within the 5' and 3'UTR of p53 mRNA creates a double-stranded RNA structure necessary for RPL26 binding to p53 mRNA. Disruption of this doublestranded RNA structure, either by base mutations in the two complementary UTR sequences or with a singlestranded oligonucleotide targeting the 5'-3'UTR base p ...
Suppression of rat collagen-induced arthritis by lentiviral vector
... component of pannus tissues in rheumatoid arthritis (RA). In tumor cells, the binding of inhibitory apoptosis stimulating protein of p53 (iASPP) with transcription factor p53 family members can inhibit the downstream apoptosis signaling. We hypothesized that iASPP molecule is involved in the RA path ...
... component of pannus tissues in rheumatoid arthritis (RA). In tumor cells, the binding of inhibitory apoptosis stimulating protein of p53 (iASPP) with transcription factor p53 family members can inhibit the downstream apoptosis signaling. We hypothesized that iASPP molecule is involved in the RA path ...
Gene Section UBE3A (ubiquitin protein ligase E3A) Atlas of Genetics and Cytogenetics
... The E6 oncoprotein of so-called high risk HPVs that have been etiologically associated with malignant lesion of the anogenital tract (most notably, cervical cancer) have the ability to bind to E6AP. The E6/E6AP complex binds to the p53 tumor suppressor, thereby targeting p53 for ubiquitylation and p ...
... The E6 oncoprotein of so-called high risk HPVs that have been etiologically associated with malignant lesion of the anogenital tract (most notably, cervical cancer) have the ability to bind to E6AP. The E6/E6AP complex binds to the p53 tumor suppressor, thereby targeting p53 for ubiquitylation and p ...
P53
Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. As such, p53 has been described as ""the guardian of the genome"" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene.The name p53 was given in 1979 describing the apparent molecular mass; SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. In addition to the full length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to 53 kDa. All these p53 proteins are called the p53 isoforms.The International Cancer Genome Consortium has established that the TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation.TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome.