Challenges in Endometrial biopsy
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
Review of the p53 Tumor Suppressor Gene and its Role in Gliomas
... In the same way, cells begin to differentiate at a ...
... In the same way, cells begin to differentiate at a ...
Nuclear and Nucleolar Localization by the N
... 36). In yeast, expression of human topo I is unhealthy and its overexpression is lethal but also dependent on the N-terminal domain (3). Most likely the N-terminus functions in nuclear targeting and since the 166-210-amino acid region of human topo I can form a complex with nucleolin, a possible mec ...
... 36). In yeast, expression of human topo I is unhealthy and its overexpression is lethal but also dependent on the N-terminal domain (3). Most likely the N-terminus functions in nuclear targeting and since the 166-210-amino acid region of human topo I can form a complex with nucleolin, a possible mec ...
A novel exon within the mdm2 gene modulates translation
... in-frame codons, including a UGA codon (Figures 1 and 2a). This additional sequence, termed the a exon, lies within the sequence encoding the p53 binding domain of mdm2. The a exon is highly conserved We next asked if human cells also express a mdm2a transcript and screened for the presence of mdm2a ...
... in-frame codons, including a UGA codon (Figures 1 and 2a). This additional sequence, termed the a exon, lies within the sequence encoding the p53 binding domain of mdm2. The a exon is highly conserved We next asked if human cells also express a mdm2a transcript and screened for the presence of mdm2a ...
p53-associated 3 exonuclease activity in nuclear and cytoplasmic
... Foord et al., 1993): (a) p53 itself has the ability to recognize and interact with several forms of DNA such as single-stranded (ss) and double-stranded (ds) DNA ends, DNA with insertion–deletion lesion mismatches (Steinmeyer and Deppert, 1988; Kern et al., 1991; Lee et al., 1995); (b) p53 promotes ...
... Foord et al., 1993): (a) p53 itself has the ability to recognize and interact with several forms of DNA such as single-stranded (ss) and double-stranded (ds) DNA ends, DNA with insertion–deletion lesion mismatches (Steinmeyer and Deppert, 1988; Kern et al., 1991; Lee et al., 1995); (b) p53 promotes ...
Review on Dengue viral Replication, assembly and entry into the
... opened new avenues that can be explored toward understanding the molecular mechanism behind E protein dynamics and the role of the immature particles in the virus life cycle. The E protein also provides the first point of contact between the virus and the host cell. Several cellular proteins and car ...
... opened new avenues that can be explored toward understanding the molecular mechanism behind E protein dynamics and the role of the immature particles in the virus life cycle. The E protein also provides the first point of contact between the virus and the host cell. Several cellular proteins and car ...
Regulation of the endothelial cell cycle by the ubiquitin
... from E2 to the target protein. Substrates can be modified with a single Ub or with Ub chains, but only poly-ubiquitination addresses proteins for degradation by the 26S-proteasome. In fact, monoubiquitination rather modulates growth factor endocytosis, PCNA activity during DNA-repair, and is involve ...
... from E2 to the target protein. Substrates can be modified with a single Ub or with Ub chains, but only poly-ubiquitination addresses proteins for degradation by the 26S-proteasome. In fact, monoubiquitination rather modulates growth factor endocytosis, PCNA activity during DNA-repair, and is involve ...
Replication of damaged DNA in vitro is blocked
... contains three copies of the RGC site (Fig. 1A and B). The abilities of the different forms of p53 protein to inhibit the replication of Vw16PyCAT in vitro as measured both by incorporation of labeled deoxynucleotide into progeny DNA (Fig. 1C) and DpnI-resistant DNA detected on a 1% vertical agarose ...
... contains three copies of the RGC site (Fig. 1A and B). The abilities of the different forms of p53 protein to inhibit the replication of Vw16PyCAT in vitro as measured both by incorporation of labeled deoxynucleotide into progeny DNA (Fig. 1C) and DpnI-resistant DNA detected on a 1% vertical agarose ...
Regulatory role of rpL3 in cell response to nucleolar stress induced
... 5nM of Act D for 24 h or untreated and analyzed by western blotting with anti-rpL3 and anti-p21 (Fig. S1). As shown in Fig. 2C-D in untreated cells the rpL3 silencing did not cause any significant alteration on p21 promoter activity compared to untreated cells transfected with scrambled siRNA. The tr ...
... 5nM of Act D for 24 h or untreated and analyzed by western blotting with anti-rpL3 and anti-p21 (Fig. S1). As shown in Fig. 2C-D in untreated cells the rpL3 silencing did not cause any significant alteration on p21 promoter activity compared to untreated cells transfected with scrambled siRNA. The tr ...
Maintenance of genomic integrity by p53: complementary
... interesting that IGF-BP3 is a target for transcriptional activation by p53. Overexpression of IGF-BP3 can inhibit the mitogenic activity of IGF receptor signaling, while in other systems it can inhibit the survival signals provided by IGF, thereby amplifying apoptosis. Thus the extracellular environ ...
... interesting that IGF-BP3 is a target for transcriptional activation by p53. Overexpression of IGF-BP3 can inhibit the mitogenic activity of IGF receptor signaling, while in other systems it can inhibit the survival signals provided by IGF, thereby amplifying apoptosis. Thus the extracellular environ ...
INHIBITING THE p53–MDM2 INTERACTION: AN IMPORTANT
... activation of p53 can lead to various responses (FIG. 1). For example, DNA damage might result in growth arrest to allow for repair of the damage, or apoptosis — both of these responses aim to prevent damaged cells from proliferating and passing mutations on to the next generation2. p53 acts as a tr ...
... activation of p53 can lead to various responses (FIG. 1). For example, DNA damage might result in growth arrest to allow for repair of the damage, or apoptosis — both of these responses aim to prevent damaged cells from proliferating and passing mutations on to the next generation2. p53 acts as a tr ...
The Nucleolus under Stress
... before and after CPT (camptothecin) treatment, a specific topoisomerase I inhibitor. This revealed rapid changes in concentration and localization of proteins involved in nucleolar stress, DNA damage, and oxidative stress pathways (Cohen et al., 2008). Interestingly, most proteins that translocated ...
... before and after CPT (camptothecin) treatment, a specific topoisomerase I inhibitor. This revealed rapid changes in concentration and localization of proteins involved in nucleolar stress, DNA damage, and oxidative stress pathways (Cohen et al., 2008). Interestingly, most proteins that translocated ...
Fanconi anemia genes act to suppress a cross-linker-inducible p53-
... medium supplemented with hygromycin (final concentration, 200 pg/mL; Boehringer, Mannheim, Germany). Cell counting was performed with a Coulter counter (Coulter Immunology, Hialeah, FL). Treatment with MMC (Kyowa Hakko Kyoga Ltd, Japan) was performed according to either one of two protocols: (1) exp ...
... medium supplemented with hygromycin (final concentration, 200 pg/mL; Boehringer, Mannheim, Germany). Cell counting was performed with a Coulter counter (Coulter Immunology, Hialeah, FL). Treatment with MMC (Kyowa Hakko Kyoga Ltd, Japan) was performed according to either one of two protocols: (1) exp ...
LEADING ARTICLE Concurrent disruption of p16INK4a and
... Alterations of several oncogenes and tumor suppressor genes have been demonstrated in NHL.3 As in many other types of cancer, the most commonly altered targets reported in NHL are the genes encoding the tumor suppressors p16INK4a and p53.4–22 These proteins are key components of the machinery that c ...
... Alterations of several oncogenes and tumor suppressor genes have been demonstrated in NHL.3 As in many other types of cancer, the most commonly altered targets reported in NHL are the genes encoding the tumor suppressors p16INK4a and p53.4–22 These proteins are key components of the machinery that c ...
Direct redox modulation of p53 protein: potential sources of redox
... Appropriate response to environmental stressors is essential for life. Many stressors, such as UV light, ionizing radiation, reactive oxygen intermediates (ROI), heat shock and hypoxia alter the redox potential of the cell. Recently, it has been shown that some of these stressors promote direct oxid ...
... Appropriate response to environmental stressors is essential for life. Many stressors, such as UV light, ionizing radiation, reactive oxygen intermediates (ROI), heat shock and hypoxia alter the redox potential of the cell. Recently, it has been shown that some of these stressors promote direct oxid ...
p53 in Hematologic Malignancies
... Most of the p53 mutations in these hematopoietic maligcan exist potentially in two conformations that may be denanciesare G:Cto A:Ttransitionalnucleotidealterations pendent on the cell cycle. This alteration of p53 conformaand most of these occur at CpG dinucleotides. This pattern tion in normal div ...
... Most of the p53 mutations in these hematopoietic maligcan exist potentially in two conformations that may be denanciesare G:Cto A:Ttransitionalnucleotidealterations pendent on the cell cycle. This alteration of p53 conformaand most of these occur at CpG dinucleotides. This pattern tion in normal div ...
Activation of Src Kinases p53/56@ and p59hckby @ in Myeloid Cells`
... IL-3-dependent cell line 32D was transfected with the plasmid pGD21O as described in “Materialsand Methods.―The resulting cell line 32Dp2l0 stably expressed the kinase; it could be grown with G4l8 (1 mg/mi) and without the addition of growth factors as described (1 1, 13). To investigate the eff ...
... IL-3-dependent cell line 32D was transfected with the plasmid pGD21O as described in “Materialsand Methods.―The resulting cell line 32Dp2l0 stably expressed the kinase; it could be grown with G4l8 (1 mg/mi) and without the addition of growth factors as described (1 1, 13). To investigate the eff ...
Golgi-SNARE GS28 potentiates cisplatin
... protein α) and CITED2 {CBP [CREB (cAMP-response-elementbinding protein)-binding protein]/p300-interacting transactivator, with glutamate/aspartate-rich C-terminal domain 2}, as being consistently overexpressed in cisplatin-resistant cells [12–14]. Notably, the products of these two ‘chemoresistance’ ...
... protein α) and CITED2 {CBP [CREB (cAMP-response-elementbinding protein)-binding protein]/p300-interacting transactivator, with glutamate/aspartate-rich C-terminal domain 2}, as being consistently overexpressed in cisplatin-resistant cells [12–14]. Notably, the products of these two ‘chemoresistance’ ...
Prostate Cancer - University of Wisconsin–Eau Claire
... •GSTp absent in almost every prostate tumor •GSTp may be only thing stopping prostate cancer ...
... •GSTp absent in almost every prostate tumor •GSTp may be only thing stopping prostate cancer ...
Transcriptional regulation of the H-ras1 proto
... (Zambetti et al, 1993, Hainaut, 1995). The mutant forms of P53 promote tumorigenesis by dominant-negative inhibition of wild-type P53 through cross-oligomerization. Moreover, P53 mutants were proved to exert oncogenic functions of their own (Dittmer et al, 1993). 1 . Regulation o f the c-H-ras by th ...
... (Zambetti et al, 1993, Hainaut, 1995). The mutant forms of P53 promote tumorigenesis by dominant-negative inhibition of wild-type P53 through cross-oligomerization. Moreover, P53 mutants were proved to exert oncogenic functions of their own (Dittmer et al, 1993). 1 . Regulation o f the c-H-ras by th ...
machinery pre-mRNA in sensing defects in the spliceosomal Mdm4
... P0 Prmt5F/FNes brains had a lower cellularity count in both the cortical plate (CP) and the ventricular zone/ subventricular zone (VZ/SVZ) (Fig. 1C) and a lower number of SOX2/Ki67-positive proliferating NPCs (Fig. 1D) as opposed to controls (PRMT5F/F). We could not observe any appreciable differenc ...
... P0 Prmt5F/FNes brains had a lower cellularity count in both the cortical plate (CP) and the ventricular zone/ subventricular zone (VZ/SVZ) (Fig. 1C) and a lower number of SOX2/Ki67-positive proliferating NPCs (Fig. 1D) as opposed to controls (PRMT5F/F). We could not observe any appreciable differenc ...
Genetic Polymorphisms in Meningioma Formation and Progression
... Discovering such associations allows for development of additional meningioma diagnostic procedures and treatments. As part of Human Genome Epidemiology, an evolving field of inquiry that applies an epidemiologic approach to studying the human genome, genetic correlations can be used to design geneti ...
... Discovering such associations allows for development of additional meningioma diagnostic procedures and treatments. As part of Human Genome Epidemiology, an evolving field of inquiry that applies an epidemiologic approach to studying the human genome, genetic correlations can be used to design geneti ...
The contribution of the Trp/Met/Phe residues to physical interactions
... domain to CBP C-terminus promotes acetylation of the p53 Cterminal lysine residues by CBP/p300. The acetylations of the lysine residues contribute to the p53 stabilization by disabling the lysines as the ubiquitylation targets. The acetylation of p53 also stimulates its sequence specific DNA-binding ...
... domain to CBP C-terminus promotes acetylation of the p53 Cterminal lysine residues by CBP/p300. The acetylations of the lysine residues contribute to the p53 stabilization by disabling the lysines as the ubiquitylation targets. The acetylation of p53 also stimulates its sequence specific DNA-binding ...
P53
Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. As such, p53 has been described as ""the guardian of the genome"" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene.The name p53 was given in 1979 describing the apparent molecular mass; SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. In addition to the full length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to 53 kDa. All these p53 proteins are called the p53 isoforms.The International Cancer Genome Consortium has established that the TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation.TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome.