Power tools for gene expression and clonal analysis in
... 18 °C but does not repress GAL4 at 29 °C or higher temperatures. Alternatively, one can use recombinases to temporally control the expression of GAL80, as we will discuss below. This threecomponent system (GAL4-UAS, transgene and GAL80) is the basis of two powerful technologies: MARCM (discussed bel ...
... 18 °C but does not repress GAL4 at 29 °C or higher temperatures. Alternatively, one can use recombinases to temporally control the expression of GAL80, as we will discuss below. This threecomponent system (GAL4-UAS, transgene and GAL80) is the basis of two powerful technologies: MARCM (discussed bel ...
A-new-precipitation-technique-provides-evidence-for-the
... the permeability of cell walls and especially of endodermal Casparian bands (CBs) for ions was tested in young roots of corn (Zea mays) and rice (Oryza sativa). The test was based on suction of either 100 mM CuSO4 or 200 mM K4[Fe(CN)6] into the root from its medium using a pump (excised roots) or tr ...
... the permeability of cell walls and especially of endodermal Casparian bands (CBs) for ions was tested in young roots of corn (Zea mays) and rice (Oryza sativa). The test was based on suction of either 100 mM CuSO4 or 200 mM K4[Fe(CN)6] into the root from its medium using a pump (excised roots) or tr ...
A Cell is Like A (100 points) You will compare an animal or plant
... ribosomes, cytoplasm, mitochondria, endoplasmic reticulum, Golgi body, cell membrane, chromosomes, lysosome, vacuole. o Plant Cell Projects MUST include the following 12 organelles: nucleus, ribosomes, cytoplasm, mitochondria, endoplasmic reticulum, Golgi body, cell membrane, chromosomes, lysosome, ...
... ribosomes, cytoplasm, mitochondria, endoplasmic reticulum, Golgi body, cell membrane, chromosomes, lysosome, vacuole. o Plant Cell Projects MUST include the following 12 organelles: nucleus, ribosomes, cytoplasm, mitochondria, endoplasmic reticulum, Golgi body, cell membrane, chromosomes, lysosome, ...
The Role of the Leukemia Inhibitory Factor (LIF)
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
Modeling the Gastric Mill Central Pattern Generator of the Lobster
... time it should be consistent with the experimental data. One has to find a balance between simplicity, which implies using the smallest possible number of parameters in a model, and faithfulness to the experimental data, which may well require an essentially unlimited number of parameters. Many soph ...
... time it should be consistent with the experimental data. One has to find a balance between simplicity, which implies using the smallest possible number of parameters in a model, and faithfulness to the experimental data, which may well require an essentially unlimited number of parameters. Many soph ...
isolation and genetic analysis of mutant strains in gametic
... by R. K. TOGASAKI and taken to Indiana University, where they were maintained in culture. Subsequently, DR. TOGASAKI sent us cells from his cultures (which we propose to designate 137c-H mt+ and 137c-H mt-). Both groups of investigators proceeded to use these as parent strains for the isolation of v ...
... by R. K. TOGASAKI and taken to Indiana University, where they were maintained in culture. Subsequently, DR. TOGASAKI sent us cells from his cultures (which we propose to designate 137c-H mt+ and 137c-H mt-). Both groups of investigators proceeded to use these as parent strains for the isolation of v ...
Chromosomal DNA demethylation specified by
... were done over a 102-day interval (this was 152 days after LacI integration). The DNA harvested was double-digested with HindIII and HhaI for methylation analysis. No demethylation at the three HhaI sites within the lacO sequence was observed throughout the experimental interval of 102 days with IPT ...
... were done over a 102-day interval (this was 152 days after LacI integration). The DNA harvested was double-digested with HindIII and HhaI for methylation analysis. No demethylation at the three HhaI sites within the lacO sequence was observed throughout the experimental interval of 102 days with IPT ...
The importance of foetal movement for co
... increased strength of bones in the dominant limbs of athletes), the role of movement in shaping the skeleton during embryogenesis has been acknowledged only relatively recently. A number of intriguing studies have emerged which have documented the clinical significance of mechanical stimulation to n ...
... increased strength of bones in the dominant limbs of athletes), the role of movement in shaping the skeleton during embryogenesis has been acknowledged only relatively recently. A number of intriguing studies have emerged which have documented the clinical significance of mechanical stimulation to n ...
Mutations in a novel gene, myoblast city, provide evidence
... they stretch and send out long processes (Fig. 1C). These two schedule at 9 hours AEL, in cells that appear by their position myoblast populations and their behaviours are reminiscent of to belong to the somatic mesoderm. Because of their position the two myoblast types postulated to exist during wi ...
... they stretch and send out long processes (Fig. 1C). These two schedule at 9 hours AEL, in cells that appear by their position myoblast populations and their behaviours are reminiscent of to belong to the somatic mesoderm. Because of their position the two myoblast types postulated to exist during wi ...
Mechanism of Inhibition of Cell Proliferation by
... Vinca alkaloids inhibit cell proliferation primarily by means of their effects on mitotic spindle microtubules or by another mechanism or by a combination of mechanisms. Five Vìnca alkaloids were used to investigate the relationship in Urla cells between inhibition of cell proliferation and blockag ...
... Vinca alkaloids inhibit cell proliferation primarily by means of their effects on mitotic spindle microtubules or by another mechanism or by a combination of mechanisms. Five Vìnca alkaloids were used to investigate the relationship in Urla cells between inhibition of cell proliferation and blockag ...
Retinoic acid-mediated activation of HNF-3a
... a specific set of genes. Some genes respond rapidly to the action of RA, indicating that their regulatory sequences are direct targets for retinoic acid receptors [8, 9]. This has been corroborated by the identification of retinoic acid response elements in a number of different promoters [10—12]. I ...
... a specific set of genes. Some genes respond rapidly to the action of RA, indicating that their regulatory sequences are direct targets for retinoic acid receptors [8, 9]. This has been corroborated by the identification of retinoic acid response elements in a number of different promoters [10—12]. I ...
- Wiley Online Library
... the employment of cytoskeletal proteins, and the use of the membrane properties, that is, curvature, electric potential, and composition, as localization signals. The most surprising mechanism discovered thus far is targeting of certain mRNAs to the subcellular domains where their protein products a ...
... the employment of cytoskeletal proteins, and the use of the membrane properties, that is, curvature, electric potential, and composition, as localization signals. The most surprising mechanism discovered thus far is targeting of certain mRNAs to the subcellular domains where their protein products a ...
Chapter 4
... Microscopes as Windows on the World of Cells – Light microscopes can be used to explore the structures and functions of cells. – When scientists examine a specimen on a microscope slide • Light passes through the specimen • Lenses enlarge, or magnify, the image – Magnification is an increase in the ...
... Microscopes as Windows on the World of Cells – Light microscopes can be used to explore the structures and functions of cells. – When scientists examine a specimen on a microscope slide • Light passes through the specimen • Lenses enlarge, or magnify, the image – Magnification is an increase in the ...
Nucleolar targeting of BN46/51 - Journal of Cell Science
... BN46/51 expressing cells were prepared as described (Brodsky et al., 1998). In earlier experiments total yeast protein used for analysis of mAbs was isolated from spheroplasts prepared as described (Kilmartin and Adams, 1984) except that the fixation step was omitted. Proteins were fractionated on 7 ...
... BN46/51 expressing cells were prepared as described (Brodsky et al., 1998). In earlier experiments total yeast protein used for analysis of mAbs was isolated from spheroplasts prepared as described (Kilmartin and Adams, 1984) except that the fixation step was omitted. Proteins were fractionated on 7 ...
Chapter Five - Building a Fly Eye: Terminal
... 2.2. Terminal differentiation of fly PRs Fly PR development occurs in two major steps: PR cell specification and terminal differentiation (Mollereau et al., 2001). PR cell specification occurs during the latest stages of larval development, and has been a topic of extensive study (for a review, see ...
... 2.2. Terminal differentiation of fly PRs Fly PR development occurs in two major steps: PR cell specification and terminal differentiation (Mollereau et al., 2001). PR cell specification occurs during the latest stages of larval development, and has been a topic of extensive study (for a review, see ...
Coca Cola
... intracellular organization Locomotion by cellular appendages (cilia and flagella) Segregation of chromosomes during mitosis ...
... intracellular organization Locomotion by cellular appendages (cilia and flagella) Segregation of chromosomes during mitosis ...
Cleavage furrow formation and ingression during animal cytokinesis
... ingression of the cleavage furrow (Inoue et al., 2004). Timelapse analysis revealed that a set of ‘peripheral’ astral microtubules contacts the cortex exactly at the cleavage site and forms overlapping antiparallel bundles that promote furrow ingression. These bundles then merge with a distinct ‘int ...
... ingression of the cleavage furrow (Inoue et al., 2004). Timelapse analysis revealed that a set of ‘peripheral’ astral microtubules contacts the cortex exactly at the cleavage site and forms overlapping antiparallel bundles that promote furrow ingression. These bundles then merge with a distinct ‘int ...
PDF
... different epithelial compartments, the hair germ and the bulge. The hair germ (light green) is located close to the mesenchymal dermal papilla (red), which acts as a signaling center. The bulge (dark green) is located further away from the dermal papilla. The cells with the most label retention are ...
... different epithelial compartments, the hair germ and the bulge. The hair germ (light green) is located close to the mesenchymal dermal papilla (red), which acts as a signaling center. The bulge (dark green) is located further away from the dermal papilla. The cells with the most label retention are ...
PD-1 promotes immune exhaustion by inducing antiviral T cell
... iors would differ in these two different microenvironments after infection (Fig. 1 A and Videos 1–3). For example, at day 4 after infection subtle differences in T cell motility had already emerged between the two models of infection (Fig. 1 B and Video 2). When compared with Arm infection, P14 cell ...
... iors would differ in these two different microenvironments after infection (Fig. 1 A and Videos 1–3). For example, at day 4 after infection subtle differences in T cell motility had already emerged between the two models of infection (Fig. 1 B and Video 2). When compared with Arm infection, P14 cell ...
Ultrastructural and Physiological Characterization of YELP, a Novel
... Disruption of the cla-1 (chloroplasts altered) gene in Arabidopsis thaliana affects the expression of the other photosynthesis-related genes encoded in the nucleus and in the chloroplast [20]. Plastids with mutations in dcl, dag and cla-1 are arrested in a state of proplastid, while some plastids of ...
... Disruption of the cla-1 (chloroplasts altered) gene in Arabidopsis thaliana affects the expression of the other photosynthesis-related genes encoded in the nucleus and in the chloroplast [20]. Plastids with mutations in dcl, dag and cla-1 are arrested in a state of proplastid, while some plastids of ...
Active Stable Maintenance Functions in Low Copy
... Abstract Active support is needed for low copy-number plasmids to be stably maintained in bacterial cells. The mechanisms that fulfill this role are (i) partition systems (PAR) acting to separate plasmid molecules to daughter cells and (ii) toxin-andidote (TA) (post-segregational killing-PSK) system ...
... Abstract Active support is needed for low copy-number plasmids to be stably maintained in bacterial cells. The mechanisms that fulfill this role are (i) partition systems (PAR) acting to separate plasmid molecules to daughter cells and (ii) toxin-andidote (TA) (post-segregational killing-PSK) system ...
Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva
... the death domains demonstrate functional heterogeneity. In the case of TRADD, its death domain is essential for binding to the death domain of TNFR1 and also for the induction of apoptosis (Hsu et al., 1995). The death domain of FADD, however, is only necessary for binding to Fas but not for the ind ...
... the death domains demonstrate functional heterogeneity. In the case of TRADD, its death domain is essential for binding to the death domain of TNFR1 and also for the induction of apoptosis (Hsu et al., 1995). The death domain of FADD, however, is only necessary for binding to Fas but not for the ind ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.