Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast
... colonies from each independent culture for further analysis. We performed initial complementation testing and found that these 24 mutants fell into several complementation groups, suggesting that suppression can be caused by a mutation in any of several different genes. To find potential causative m ...
... colonies from each independent culture for further analysis. We performed initial complementation testing and found that these 24 mutants fell into several complementation groups, suggesting that suppression can be caused by a mutation in any of several different genes. To find potential causative m ...
Transcriptome Analysis of Proliferating
... schemes. Since both gave similar insight into our microarray data, we present the analysis based only on the TAIR GO terms. However, complete analysis using both vocabularies is provided in Supplemental Tables S4 to S7. Analysis of the EP partition indicated enrichment for GO terms associated with t ...
... schemes. Since both gave similar insight into our microarray data, we present the analysis based only on the TAIR GO terms. However, complete analysis using both vocabularies is provided in Supplemental Tables S4 to S7. Analysis of the EP partition indicated enrichment for GO terms associated with t ...
1 METT-10, A Putative Methyltransferase, Inhibits Germ
... zone, but not in the adjacent meiotic region (Figure 1B). The suspected ectopic proliferating cells in mett-10(oz36) mutants label with EdU within a 3 hour period, showing that they are cycling (Figure 1C). Because EdU labels cells in both mitotic and meiotic S-phase, we also stained for additional ...
... zone, but not in the adjacent meiotic region (Figure 1B). The suspected ectopic proliferating cells in mett-10(oz36) mutants label with EdU within a 3 hour period, showing that they are cycling (Figure 1C). Because EdU labels cells in both mitotic and meiotic S-phase, we also stained for additional ...
Chemical genetics discloses the importance of heme
... Chlamydiae are pathogens that cause infections in humans and animals (Horn 2008). In humans, more than 100 million new chlamydiae infections are estimated each year (Batteiger 2012). The taxonomy is confusing (Stephens, Myers et al. 2009) and therefore I will refer to chlamydiae, which in fact inclu ...
... Chlamydiae are pathogens that cause infections in humans and animals (Horn 2008). In humans, more than 100 million new chlamydiae infections are estimated each year (Batteiger 2012). The taxonomy is confusing (Stephens, Myers et al. 2009) and therefore I will refer to chlamydiae, which in fact inclu ...
Lats kinase is involved in the intestinal apical
... in the fly, the Hpo pathway regulates differentiation of the polar follicle cell (PFC). Mutations in the core Hpo pathway genes in the PFC lead to a misoriented mitotic spindle, a multilayered epithelium and abnormal cell polarity, consistent with the Hpo pathway being involved in the regulation of ...
... in the fly, the Hpo pathway regulates differentiation of the polar follicle cell (PFC). Mutations in the core Hpo pathway genes in the PFC lead to a misoriented mitotic spindle, a multilayered epithelium and abnormal cell polarity, consistent with the Hpo pathway being involved in the regulation of ...
Complex networks orchestrate epithelial–mesenchymal transitions
... Tissue-culture studies have been instrumental in defining the molecular regulation of EMT. These studies show that several extracellular activators can trigger EMT, that extensive crosstalk exists between the signalling pathways that activate and repress EMT, and that EMT-inducing signalling pathway ...
... Tissue-culture studies have been instrumental in defining the molecular regulation of EMT. These studies show that several extracellular activators can trigger EMT, that extensive crosstalk exists between the signalling pathways that activate and repress EMT, and that EMT-inducing signalling pathway ...
Phospholipase D promotes Arcanobacterium haemolyticum
... PLDs are ubiquitous enzymes which cleave phospholipids, including phosphatidylcholine (PC) and sphingomyelin (SM), both of which are abundant in the mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, memb ...
... PLDs are ubiquitous enzymes which cleave phospholipids, including phosphatidylcholine (PC) and sphingomyelin (SM), both of which are abundant in the mammalian plasma membrane [17]. SM, with cholesterol and GPI-anchored proteins, predominantly partitions to lipid rafts, which are tightly packed, memb ...
Slam and germ cell migration - Development
... that contact germ cells during their migration, or more directly in the production or localization of a guidance cue. As the migration defect is first observed when the germ cells leave the midgut, we analyzed the development of the midgut and the gonadal mesoderm in mutant embryos. In situ hybridiz ...
... that contact germ cells during their migration, or more directly in the production or localization of a guidance cue. As the migration defect is first observed when the germ cells leave the midgut, we analyzed the development of the midgut and the gonadal mesoderm in mutant embryos. In situ hybridiz ...
151 Compact cell image projector
... 3 s; two patterns of double pulses: c) DP1000, in which stimuli were separated by alternating intervals of 1 and 3 s; and, d) DP700, in which stimuli were separated by alternating intervals of 0.7 and 3.3 s (Figure 2). Thus, cells were stimulated at the constant average frequency of 0.5 Hz, but with ...
... 3 s; two patterns of double pulses: c) DP1000, in which stimuli were separated by alternating intervals of 1 and 3 s; and, d) DP700, in which stimuli were separated by alternating intervals of 0.7 and 3.3 s (Figure 2). Thus, cells were stimulated at the constant average frequency of 0.5 Hz, but with ...
comparative study of extracellular fibrils on the ectodermal layer in
... observed similar fibrils in the flank region of neurulae of several different species. He also observed that there are many fewer fibrils in R. palustris embryos than in A. maculatum embryos. Johnson, Silver & Kelley (1979) also failed to observe discrete fibrillar structures on the inner aspect of ...
... observed similar fibrils in the flank region of neurulae of several different species. He also observed that there are many fewer fibrils in R. palustris embryos than in A. maculatum embryos. Johnson, Silver & Kelley (1979) also failed to observe discrete fibrillar structures on the inner aspect of ...
The relative roles of specific N- and C
... B). Consistent with this correlation, fractionation of this kinase on a molecular sieve column (Sephacryl S-200) with proteins of known molecular mass also suggested that the kinase had an apparent molecular mass of 37 kDa (Fig. 1C). Additional evidence that the 37 kDa protein was a protein kinase c ...
... B). Consistent with this correlation, fractionation of this kinase on a molecular sieve column (Sephacryl S-200) with proteins of known molecular mass also suggested that the kinase had an apparent molecular mass of 37 kDa (Fig. 1C). Additional evidence that the 37 kDa protein was a protein kinase c ...
Auxin transport through non-hair cells sustains root
... Auxin distribution is controlled by the movement of auxin into and out of individual cells. Protonated auxin enters cells from the cell wall by diffusion, while the ion enters via the activity of AUX1/LAX auxin-influx facilitators. AUX1 activity increases efficiency of auxin uptake compared to diffu ...
... Auxin distribution is controlled by the movement of auxin into and out of individual cells. Protonated auxin enters cells from the cell wall by diffusion, while the ion enters via the activity of AUX1/LAX auxin-influx facilitators. AUX1 activity increases efficiency of auxin uptake compared to diffu ...
Effector-triggered defence against apoplastic fungal pathogens
... PTI is rapidly activated by extracellular host recognition of PAMPs by pattern recognition receptors (PRRs); most PRRs are receptor-like kinases with an extracellular receptor domain, a transmembrane domain, and typically a cytoplasmic kinase domain. Pathogens produce effectors to suppress PTI and c ...
... PTI is rapidly activated by extracellular host recognition of PAMPs by pattern recognition receptors (PRRs); most PRRs are receptor-like kinases with an extracellular receptor domain, a transmembrane domain, and typically a cytoplasmic kinase domain. Pathogens produce effectors to suppress PTI and c ...
One-eyed pinhead regulates cell motility independent of Squint
... comes up between 4 and 5 hours postfertilization, initially overlapping with the maternal pattern of oep transcripts. At this time, both transcripts are uniformly expressed throughout the zebrafish blastoderm. However, by 5– 6 h, before the onset of gastrulation, expression becomes restricted to the ...
... comes up between 4 and 5 hours postfertilization, initially overlapping with the maternal pattern of oep transcripts. At this time, both transcripts are uniformly expressed throughout the zebrafish blastoderm. However, by 5– 6 h, before the onset of gastrulation, expression becomes restricted to the ...
p.1 MicroRNA miR-144 modulates oxidative stress
... In this report, we have analyzed the erythrocyte microRNAs to identify a group of SCD patients with a more severe anemic phenotype. We have found that high erythrocytic miR-144 expression is associated with severe anemia in SCD and describe a potential mechanism supporting this association. Increase ...
... In this report, we have analyzed the erythrocyte microRNAs to identify a group of SCD patients with a more severe anemic phenotype. We have found that high erythrocytic miR-144 expression is associated with severe anemia in SCD and describe a potential mechanism supporting this association. Increase ...
TBX5 is required for embryonic cardiac cell cycle - UNC
... proliferation begins after cardiac cell commitment, and therefore occurs coincident with, or shortly after, gastrulation, a period when the cardiac precursor cells are positioned in two populations on both sides of the organizer or node. Once cells are committed to the cardiac lineage, the two popul ...
... proliferation begins after cardiac cell commitment, and therefore occurs coincident with, or shortly after, gastrulation, a period when the cardiac precursor cells are positioned in two populations on both sides of the organizer or node. Once cells are committed to the cardiac lineage, the two popul ...
ANSWER
... • ANSWER: this is the only organelle without a membrane, it is the site where proteins are made. • QUESTION: What is the ribosome? ...
... • ANSWER: this is the only organelle without a membrane, it is the site where proteins are made. • QUESTION: What is the ribosome? ...
View PDF - G3: Genes | Genomes | Genetics
... that affect entry into and recovery from telomere-driven senescence, we combined a gene deletion disrupting telomerase (est1D) with the systematic yeast deletion collection and measured senescence characteristics in high-throughput assays. As expected, the vast majority of gene deletions showed no s ...
... that affect entry into and recovery from telomere-driven senescence, we combined a gene deletion disrupting telomerase (est1D) with the systematic yeast deletion collection and measured senescence characteristics in high-throughput assays. As expected, the vast majority of gene deletions showed no s ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultur ...
... and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultur ...
Enhancement of photovoltaic performance of quasi-solid state dye sensitized solar cell
... performs particularly well when sensitized with Rose Bengal [22]. Being an organic dye, there are no concerns about environmental damage, as it does not contain any noble metal, such as ruthenium. RB dye has been successfully used by several groups for DSSCs structures. It has high extinction coeffi ...
... performs particularly well when sensitized with Rose Bengal [22]. Being an organic dye, there are no concerns about environmental damage, as it does not contain any noble metal, such as ruthenium. RB dye has been successfully used by several groups for DSSCs structures. It has high extinction coeffi ...
Krp1, a novel kelch related protein that is involved in pseudopod
... membrane rue-like structures at their tips. Pseudopodia have previously been shown to be implicated in motility and invasion of tumour cells, highlighting the importance of cytoskeletal reorganization in invasion (Guirguis et al., 1987; Hay et al., 1989; Lamb et al., 1997b; Liotta et al., 1991b; Na ...
... membrane rue-like structures at their tips. Pseudopodia have previously been shown to be implicated in motility and invasion of tumour cells, highlighting the importance of cytoskeletal reorganization in invasion (Guirguis et al., 1987; Hay et al., 1989; Lamb et al., 1997b; Liotta et al., 1991b; Na ...
Nucleolar localization of an isoform of the IGF
... this as an effect of the strength of the secretory signal peptide in competition with the nuclear and nucleolar localization signal(s). To exclude the possibility that the exon 2encoded signal peptide was directly involved in the nuclear and nucleolar localization, it was deleted from the constructi ...
... this as an effect of the strength of the secretory signal peptide in competition with the nuclear and nucleolar localization signal(s). To exclude the possibility that the exon 2encoded signal peptide was directly involved in the nuclear and nucleolar localization, it was deleted from the constructi ...
CDP1, a novel component of chloroplast division site
... overexpressing AtCDP1 were heterogeneous and the division site was misplaced (Figure 1A, 1B). Although two gene structures of AtCDP1 were present in the NCBI database (Figure 2M), only one of them was obtained by screening the expression library (shown with a black asterisk in Figure 2M). Moreover, ...
... overexpressing AtCDP1 were heterogeneous and the division site was misplaced (Figure 1A, 1B). Although two gene structures of AtCDP1 were present in the NCBI database (Figure 2M), only one of them was obtained by screening the expression library (shown with a black asterisk in Figure 2M). Moreover, ...
The epithelial junction: bridge, gate, and fence.
... by observing no voltage step (because everyone then assumed the resistance between different epithelial cells to be infinite). Instead, they found to their astonishment that the voltage step was nearly as large as when both microelectrodes were in the same cell!. Evidently, there were some bridge-li ...
... by observing no voltage step (because everyone then assumed the resistance between different epithelial cells to be infinite). Instead, they found to their astonishment that the voltage step was nearly as large as when both microelectrodes were in the same cell!. Evidently, there were some bridge-li ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.