hnRNPLL - Shirley Liu Lab
... the basal sIghg2b:mIghg2b ratio to be 45:1 in MPC11 cells, a ratio consistent with previous data on plasmacytoma cell lines (4). To deplete hnRNPLL protein in MPC11 cells, we transduced MPC11 cells with pLKO.1 lentivirus encoding short hairpins (shRNAs) that target Hnrpll transcripts. After eliminat ...
... the basal sIghg2b:mIghg2b ratio to be 45:1 in MPC11 cells, a ratio consistent with previous data on plasmacytoma cell lines (4). To deplete hnRNPLL protein in MPC11 cells, we transduced MPC11 cells with pLKO.1 lentivirus encoding short hairpins (shRNAs) that target Hnrpll transcripts. After eliminat ...
Loss of Growth Factor Dependence and Conversion of Transforming
... factors such as EGF and insulin, IGF-I or IGF-II, promote the transit of BALB/C-3T3 cells through G, and their entry into the S phase of the cell cycle (1, 3, 4). Competence factors act before progression factors in BALB/C-3T3 cells. GFs from the competence and progression families exert their effec ...
... factors such as EGF and insulin, IGF-I or IGF-II, promote the transit of BALB/C-3T3 cells through G, and their entry into the S phase of the cell cycle (1, 3, 4). Competence factors act before progression factors in BALB/C-3T3 cells. GFs from the competence and progression families exert their effec ...
Full Text - Verlag der Zeitschrift für Naturforschung
... (INA-1w cells) were cultured as described by Kadono et al. (2006). Briefly, the culture medium was prepared with a yeast extract-based nutrition tablet (1 EBIOS tablet/l; Asahi Food & Healthcare, Tokyo, Japan). The culture medium was re-newed every 2 weeks. One nutrition tablet (250 mg) contains 94. ...
... (INA-1w cells) were cultured as described by Kadono et al. (2006). Briefly, the culture medium was prepared with a yeast extract-based nutrition tablet (1 EBIOS tablet/l; Asahi Food & Healthcare, Tokyo, Japan). The culture medium was re-newed every 2 weeks. One nutrition tablet (250 mg) contains 94. ...
Chapter 3—Cells
... The Golgi complex packages and distributes proteins. It packages proteins in small bubbles made of a piece of membrane. The bubbles then break off and transport their contents to other parts of the cell. ...
... The Golgi complex packages and distributes proteins. It packages proteins in small bubbles made of a piece of membrane. The bubbles then break off and transport their contents to other parts of the cell. ...
DLG5 in Cell Polarity Maintenance and Cancer Development
... have self-association potential and can bind to the N-terminus of DL5 [22]. The characteristics of the coiled coil, PDZ3-PDZ4 and SH3-GUK domains suggest that DLG5 can self-associate and form a large protein complex containing multiple β-cateninbinding domains. Since DLG5 can interact with N-cadheri ...
... have self-association potential and can bind to the N-terminus of DL5 [22]. The characteristics of the coiled coil, PDZ3-PDZ4 and SH3-GUK domains suggest that DLG5 can self-associate and form a large protein complex containing multiple β-cateninbinding domains. Since DLG5 can interact with N-cadheri ...
Research Interests
... Research Interests - David R. Shook The Evolution of Morphogenic Machines The genetic pathways regulating embryonic patterning are well conserved across the metazoans, as are phylotypic body plans. But surprisingly, the morphogenic mechanisms, directed by the patterning pathways to shape that body p ...
... Research Interests - David R. Shook The Evolution of Morphogenic Machines The genetic pathways regulating embryonic patterning are well conserved across the metazoans, as are phylotypic body plans. But surprisingly, the morphogenic mechanisms, directed by the patterning pathways to shape that body p ...
Mitochondrial involvement in tracheary element
... lobing of the nucleus, nuclear DNA fragmentation, and disassembly into apoptotic bodies.1 Participation of mitochondria in apoptosis is based on observations using a cell free system for apoptosis,2 on immunolocalization of apoptotic and anti-apoptotic factors to the outer mitochondrial membrane,3 a ...
... lobing of the nucleus, nuclear DNA fragmentation, and disassembly into apoptotic bodies.1 Participation of mitochondria in apoptosis is based on observations using a cell free system for apoptosis,2 on immunolocalization of apoptotic and anti-apoptotic factors to the outer mitochondrial membrane,3 a ...
Exocytosis and Endocytosis
... and withdraw patches of the plasma membrane New membrane proteins and lipids are made in the ER, modified in Golgi bodies, and form vesicles that fuse with plasma membrane ...
... and withdraw patches of the plasma membrane New membrane proteins and lipids are made in the ER, modified in Golgi bodies, and form vesicles that fuse with plasma membrane ...
Chapter 7 Cellular Structure and Function HUMAN SKIN HUMAN
... For centuries, scientists had no idea that the human body consists of tril–lions of cells. Cells are so small that their existence was unknown before the invention of the microscope. In 1665, as indicated in Figure 7.1, an English scientist named Robert Hooke made a simple microscope and looked at a ...
... For centuries, scientists had no idea that the human body consists of tril–lions of cells. Cells are so small that their existence was unknown before the invention of the microscope. In 1665, as indicated in Figure 7.1, an English scientist named Robert Hooke made a simple microscope and looked at a ...
Regulation of developmental timing and germ cell proliferation in C
... also found that UV, similar to ionizing radiation, activates CEP-1-dependent apoptosis in the germline (Figure 2b). Given this strong CEP-1-dependent checkpoint response to UV, we sought to characterize the genes regulated by CEP-1 in response to this stress. A large number of genes are activated an ...
... also found that UV, similar to ionizing radiation, activates CEP-1-dependent apoptosis in the germline (Figure 2b). Given this strong CEP-1-dependent checkpoint response to UV, we sought to characterize the genes regulated by CEP-1 in response to this stress. A large number of genes are activated an ...
Cytoskeleton-Plasma Membrane-Cell Wall
... ECM in its vicinity that is often produced by other cells, occasionally at discrete domains known as focal adhesions (Critchley, 2000) or tight/adherens junctions and desmosomes in the case of cell-to-cell contacts (Geiger and Bershadsky, 2001). Entering the post-genomic era, contemporary cell biolo ...
... ECM in its vicinity that is often produced by other cells, occasionally at discrete domains known as focal adhesions (Critchley, 2000) or tight/adherens junctions and desmosomes in the case of cell-to-cell contacts (Geiger and Bershadsky, 2001). Entering the post-genomic era, contemporary cell biolo ...
Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan
... depend on how permeable they are. The permeabilities of these antibiotics may have been altered in the affected cells for unknown reasons. Conversely, the inhibition of chloroplast division may be due to a different effect of these antibiotics. There are two reports on the relationship between these ...
... depend on how permeable they are. The permeabilities of these antibiotics may have been altered in the affected cells for unknown reasons. Conversely, the inhibition of chloroplast division may be due to a different effect of these antibiotics. There are two reports on the relationship between these ...
Three-Dimensional Reconstruction of Tubular Structure of Vacuolar
... observed in the equatorial region (180 min, arrowheads), and tended to align in the longitudinal direction. At cytokinesis (200 min), the TVMs divided into the two daughter cells because of centrifugal expansion of the cell plate developing within the phragmoplast. After completion of cytokinesis, s ...
... observed in the equatorial region (180 min, arrowheads), and tended to align in the longitudinal direction. At cytokinesis (200 min), the TVMs divided into the two daughter cells because of centrifugal expansion of the cell plate developing within the phragmoplast. After completion of cytokinesis, s ...
Break of symmetry in regenerating tobacco protoplasts is
... member OsKCH as well as the tobacco member NtKCH have been shown to modulate premitotic nuclear positioning in tobacco BY-2 (Frey et al. 2010; Klotz and Nick 2012). To understand the role of nuclear migration in cell polarity, two factors of KCH play an important role: KCH exists in two functionally ...
... member OsKCH as well as the tobacco member NtKCH have been shown to modulate premitotic nuclear positioning in tobacco BY-2 (Frey et al. 2010; Klotz and Nick 2012). To understand the role of nuclear migration in cell polarity, two factors of KCH play an important role: KCH exists in two functionally ...
Cryopreservation of Cultured Plant Cells
... Undifferentiated cell-suspension cultures are important genetic resources used extensively in cytological, physiological, biochemical, and molecular biological studies. Cellsuspension cultures are homogeneous populations of nearly identical cells and provide advantages for investigating cellular pro ...
... Undifferentiated cell-suspension cultures are important genetic resources used extensively in cytological, physiological, biochemical, and molecular biological studies. Cellsuspension cultures are homogeneous populations of nearly identical cells and provide advantages for investigating cellular pro ...
RNA-dependent disassembly of nuclear bodies
... molecules within nucleoli are not as mobile as nucleolar proteins (Grob et al., 2014). FRAP analysis revealed that the rate of Men ε/β RNA exchange was significantly lower than that of paraspeckle proteins (Mao et al., 2011b). Maintenance of NBs appears to require a constant influx of new RNA molec ...
... molecules within nucleoli are not as mobile as nucleolar proteins (Grob et al., 2014). FRAP analysis revealed that the rate of Men ε/β RNA exchange was significantly lower than that of paraspeckle proteins (Mao et al., 2011b). Maintenance of NBs appears to require a constant influx of new RNA molec ...
gamma-delta T cells target the red blood cell
... as phosphoantigens, independent of processing or presentation by professional antigen presenting cells.5 The most active phosphoantigens are intermediate products of the deoxy-xylulose-5-phosphate (DOXP) isoprenoid synthesis pathway, which occurs in the Plasmodium apicoplast.6,7 During a primary mal ...
... as phosphoantigens, independent of processing or presentation by professional antigen presenting cells.5 The most active phosphoantigens are intermediate products of the deoxy-xylulose-5-phosphate (DOXP) isoprenoid synthesis pathway, which occurs in the Plasmodium apicoplast.6,7 During a primary mal ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.