Metabolomics of a single vacuole reveals metabolic dynamism in an
... acting as a micropipette under a microscope to isolate the molecular content from the cytoplasm of a live single cell, which was then examined by mass spectrometry. Although this technique may become an important method with future technical advances, it is presently impossible to apply this techniq ...
... acting as a micropipette under a microscope to isolate the molecular content from the cytoplasm of a live single cell, which was then examined by mass spectrometry. Although this technique may become an important method with future technical advances, it is presently impossible to apply this techniq ...
Mitotic Block Induced in HeLa Cells by Low Concentrations of
... cells had exited mitosis into an apparent interphase state (as deter mined by decondensation of chromosomes and reformation of nuclear membrane, described further below and in Fig. 5). For example, 24 h after 10 nM paclitaxel was removed from the medium, the percentage of cells in mitosis was reduce ...
... cells had exited mitosis into an apparent interphase state (as deter mined by decondensation of chromosomes and reformation of nuclear membrane, described further below and in Fig. 5). For example, 24 h after 10 nM paclitaxel was removed from the medium, the percentage of cells in mitosis was reduce ...
In vitro–expanded human CD4 CD25 T-regulatory
... microbeads (2 L/107 cells; Miltenyi Biotec, Auburn, CA), and purified over an LS⫹ column. Cells were then applied to a second magnetic column, washed, and eluted again. After the double-column procedure, cells were routinely more than 93% pure (for CD25) by FACS analysis. Alternatively, cells were ...
... microbeads (2 L/107 cells; Miltenyi Biotec, Auburn, CA), and purified over an LS⫹ column. Cells were then applied to a second magnetic column, washed, and eluted again. After the double-column procedure, cells were routinely more than 93% pure (for CD25) by FACS analysis. Alternatively, cells were ...
PPT5 - Ycmou
... Endospores are probably the most durable cell produced in nature. They are formed by vegetative cells in response to environmental signals that indicate a limiting factor for vegetative growth, such as depletion of an essential nutrient. They are highly resistant to environmental stresses such ...
... Endospores are probably the most durable cell produced in nature. They are formed by vegetative cells in response to environmental signals that indicate a limiting factor for vegetative growth, such as depletion of an essential nutrient. They are highly resistant to environmental stresses such ...
Remapping by hippocampal place cells
... Taking a slightly different view, in their elegant discussion of spatial context, Nadel and Willner (1980) argue that context is paradoxical: it both “is made up of” and “contains” the same stimuli. In other words, a given stimulus could be either a discrete cue or part of the context, depending on ...
... Taking a slightly different view, in their elegant discussion of spatial context, Nadel and Willner (1980) argue that context is paradoxical: it both “is made up of” and “contains” the same stimuli. In other words, a given stimulus could be either a discrete cue or part of the context, depending on ...
2010 - Department of Biology
... adult workers; RC3 refers to loss of the sperm storage organ (spermatheca); RC4 refers to the reduction of ovariole number in the workers; and finally, RC5 refers to the complete loss of worker reproductive organs. Our general goal is to analyse the molecular and developmental basis of these constra ...
... adult workers; RC3 refers to loss of the sperm storage organ (spermatheca); RC4 refers to the reduction of ovariole number in the workers; and finally, RC5 refers to the complete loss of worker reproductive organs. Our general goal is to analyse the molecular and developmental basis of these constra ...
Protists junior
... Protists are very small organisms, generally made up of just one cell, that have to take care of all the activities required to live: feeding, movement and reproduction. Protists include micro-organisms with very different shapes, structures and lifestyles. All protists can reproduce “asexually”, th ...
... Protists are very small organisms, generally made up of just one cell, that have to take care of all the activities required to live: feeding, movement and reproduction. Protists include micro-organisms with very different shapes, structures and lifestyles. All protists can reproduce “asexually”, th ...
Discs large 5, an Essential Gene in Drosophila, Regulates Egg
... detected in 40% of ovarioles (150 ovarioles) (Figure 2C). Transient ...
... detected in 40% of ovarioles (150 ovarioles) (Figure 2C). Transient ...
Fine mapping of re-arranged Y chromosome in three infertile
... Patsalis et al., 2005; Siffroi et al., 2000). The proportion of 45,X cells is highly variable between individuals, ranging from 1% to 90%, and can also differ between the different cell lineages within the same individual. These mosaic karyotypes are associated with a wide spectrum of clinical pheno ...
... Patsalis et al., 2005; Siffroi et al., 2000). The proportion of 45,X cells is highly variable between individuals, ranging from 1% to 90%, and can also differ between the different cell lineages within the same individual. These mosaic karyotypes are associated with a wide spectrum of clinical pheno ...
Science Jeopardy - Broward County Public Schools
... • ANSWER: Explain the difference between a cell membrane and a cell wall. • QUESTION: Cell membranes control what passes in and out of a cell, whereas a cell wall gives a cell a rigid shape. ...
... • ANSWER: Explain the difference between a cell membrane and a cell wall. • QUESTION: Cell membranes control what passes in and out of a cell, whereas a cell wall gives a cell a rigid shape. ...
Tese Hugo Fraga
... The Brazilian conifer Araucaria angustifolia naturally occurs in the Atlantic Forest Biome, and is popularly known as araucaria or Brazilianpine. According to the IUCN, this species is critically endangered, reinforcing the need for further studies aimed at their preservation and propagation. Plant ...
... The Brazilian conifer Araucaria angustifolia naturally occurs in the Atlantic Forest Biome, and is popularly known as araucaria or Brazilianpine. According to the IUCN, this species is critically endangered, reinforcing the need for further studies aimed at their preservation and propagation. Plant ...
The membrane form of the DNA repair protein Ku interacts at the cell
... component of mammalian DNA DSB repair pathway (Jackson, 2002). Accordingly, mice with deletions of Ku genes are hypersensitive to ionizing radiation and lack mature lymphocytes (for review, see Jackson, 2002). Although Ku appears to be predominantly nuclear, several reports point out the presence of ...
... component of mammalian DNA DSB repair pathway (Jackson, 2002). Accordingly, mice with deletions of Ku genes are hypersensitive to ionizing radiation and lack mature lymphocytes (for review, see Jackson, 2002). Although Ku appears to be predominantly nuclear, several reports point out the presence of ...
How to build a grid cell
... are unlikely to oscillate independently at different frequencies over sufficiently long periods of time [18], making it more likely that the VCOs are driven by synaptic inputs from direction-sensitive neurons [15,19]. At any rate, action potential firing in this type of model is produced when the VC ...
... are unlikely to oscillate independently at different frequencies over sufficiently long periods of time [18], making it more likely that the VCOs are driven by synaptic inputs from direction-sensitive neurons [15,19]. At any rate, action potential firing in this type of model is produced when the VC ...
c - SCHOOLinSITES
... • Hyperthermophiles—organisms that can survive at temperatures exceeding 100o C, such as near deep-sea vents • Examples: – Pyrolobus fumarii (“fire lobe of the chimney”) is chemosynthetic, and grows at temperatures between 90o and 113o C – “strain 121” survived 2 hours of incubation at 130o C © 2006 ...
... • Hyperthermophiles—organisms that can survive at temperatures exceeding 100o C, such as near deep-sea vents • Examples: – Pyrolobus fumarii (“fire lobe of the chimney”) is chemosynthetic, and grows at temperatures between 90o and 113o C – “strain 121” survived 2 hours of incubation at 130o C © 2006 ...
A proteomic chronology of gene expression through the cell cycle in
... characterization of gene expression in human cells, including cell cycle-associated changes in the proteome and transcriptome. We thus address the key question of how different layers of gene expression affect corresponding levels of protein and mRNA in a biologically important, dynamic system. The ...
... characterization of gene expression in human cells, including cell cycle-associated changes in the proteome and transcriptome. We thus address the key question of how different layers of gene expression affect corresponding levels of protein and mRNA in a biologically important, dynamic system. The ...
Cellular Maturation Defects in Bruton`s Tyrosine
... the pre-BCR signaling molecules Syk or p85␣ do not enter the expansion phase (reviewed in Ref. 3). In contrast, absence of the pre-BCR signaling proteins Btk and BLNK/SLP-65 was shown to be associated with defects in cell cycle exit, resulting in a high incidence of pre-B cell tumors (4 – 8). In SLP ...
... the pre-BCR signaling molecules Syk or p85␣ do not enter the expansion phase (reviewed in Ref. 3). In contrast, absence of the pre-BCR signaling proteins Btk and BLNK/SLP-65 was shown to be associated with defects in cell cycle exit, resulting in a high incidence of pre-B cell tumors (4 – 8). In SLP ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
... titer of Fl-specific antibody in B10, B10.A, and BALB.B mice (Fig. 2). Both antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody ...
... titer of Fl-specific antibody in B10, B10.A, and BALB.B mice (Fig. 2). Both antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody ...
the peroxisomal endomembrane system and the role of the ER
... ER (Beevers, 1979). These nascent proteins were proposed to then be sequestered into an expanding vesicle that would eventually bud from the ER to produce a mature, functional peroxisome (Beevers, 1979). However, subsequent observations suggested that peroxisomal proteins were not synthesized on the ...
... ER (Beevers, 1979). These nascent proteins were proposed to then be sequestered into an expanding vesicle that would eventually bud from the ER to produce a mature, functional peroxisome (Beevers, 1979). However, subsequent observations suggested that peroxisomal proteins were not synthesized on the ...
Significance of hydrolytic enzymes expressed during xylem
... and tracheids evolved independently and are not homologous (Ligrone et al. 2000, Ligrone et al. 2002). The extinct protracheophytes such as Aglaophyton were found to have a central vascular cylinder, but the conducting cells lacked secondary thickenings (Edwards 1986). As the protoplast of a living ...
... and tracheids evolved independently and are not homologous (Ligrone et al. 2000, Ligrone et al. 2002). The extinct protracheophytes such as Aglaophyton were found to have a central vascular cylinder, but the conducting cells lacked secondary thickenings (Edwards 1986). As the protoplast of a living ...
3 Ultradian Growth Oscillations in Organs: Physiological Signal or
... into an explicit model 40 years ago (Israelsson and Johnsson 1967), is that a stem responds gravitropically, overshoots its target angle, bends again, and overshoots again, thus creating an oscillation. Although the occurrence of circumnutation in space flight where gravitational force is all but ab ...
... into an explicit model 40 years ago (Israelsson and Johnsson 1967), is that a stem responds gravitropically, overshoots its target angle, bends again, and overshoots again, thus creating an oscillation. Although the occurrence of circumnutation in space flight where gravitational force is all but ab ...
Vacuolar Function in the Phosphate Homeostasis of the Yeast
... (Fig. 2A). The amounts of cytosolic phosphorous comResults pounds did not change during phosphate starvation, inDeprivation of phosphate is a critical situation to liv- dicating that the amount of inorganic phosphate was kept ing organisms. We examined growth response of yeast to constant (Fig. 2A). ...
... (Fig. 2A). The amounts of cytosolic phosphorous comResults pounds did not change during phosphate starvation, inDeprivation of phosphate is a critical situation to liv- dicating that the amount of inorganic phosphate was kept ing organisms. We examined growth response of yeast to constant (Fig. 2A). ...