Control de la estabilidad de ciclinas de G1 por nutrientes Sara
... hen environmental conditions change, Saccharomyces cerevisiae, like other organisms, takes several coordinated decisions about growth, cell division, and maintaining homeostasis. Nutrient status is among the most important environmental conditions that must be accurately sensed and responded to in o ...
... hen environmental conditions change, Saccharomyces cerevisiae, like other organisms, takes several coordinated decisions about growth, cell division, and maintaining homeostasis. Nutrient status is among the most important environmental conditions that must be accurately sensed and responded to in o ...
Imaging neural crest cell dynamics during formation
... cell arrangements, suggesting that a sophisticated set of underlying patterning mechanisms and extrinsic cues play a role in sculpting the migration pattern (Kulesa and Fraser, 1998; Teddy and Kulesa, 2004). By contrast, there is a paucity of knowledge on the migratory behaviors of neural crest cell ...
... cell arrangements, suggesting that a sophisticated set of underlying patterning mechanisms and extrinsic cues play a role in sculpting the migration pattern (Kulesa and Fraser, 1998; Teddy and Kulesa, 2004). By contrast, there is a paucity of knowledge on the migratory behaviors of neural crest cell ...
Control of the Cell Cycle in Early Embryos
... by elevated pH. Oocytes were lysed in buffer held at the prefertilization pH of 6.8, and a 12,000g supernatant was prepared. Aliquots of the lysate were mixed with an equal volume of pH 6.8 or pH 8.0 buffer, and histone H1 kinase activity was assayed at 0 and 60 min later. oocytes (Swenson et al. 19 ...
... by elevated pH. Oocytes were lysed in buffer held at the prefertilization pH of 6.8, and a 12,000g supernatant was prepared. Aliquots of the lysate were mixed with an equal volume of pH 6.8 or pH 8.0 buffer, and histone H1 kinase activity was assayed at 0 and 60 min later. oocytes (Swenson et al. 19 ...
Sodium Current Density Correlates with Expression of Specific
... by autoradiography (Fig. IB) indicated that para transcripts including the 21 amino acid exon a, as well as transcripts excluding exon a, were expressed in many cells. However, we also observed additional bands from some cells (Fig. lB), suggesting the presence of a previously uncharacterized altern ...
... by autoradiography (Fig. IB) indicated that para transcripts including the 21 amino acid exon a, as well as transcripts excluding exon a, were expressed in many cells. However, we also observed additional bands from some cells (Fig. lB), suggesting the presence of a previously uncharacterized altern ...
Imaging neural crest cell dynamics during formation
... cell arrangements, suggesting that a sophisticated set of underlying patterning mechanisms and extrinsic cues play a role in sculpting the migration pattern (Kulesa and Fraser, 1998; Teddy and Kulesa, 2004). By contrast, there is a paucity of knowledge on the migratory behaviors of neural crest cell ...
... cell arrangements, suggesting that a sophisticated set of underlying patterning mechanisms and extrinsic cues play a role in sculpting the migration pattern (Kulesa and Fraser, 1998; Teddy and Kulesa, 2004). By contrast, there is a paucity of knowledge on the migratory behaviors of neural crest cell ...
PDF - Bezanilla Lab
... plants lacking GFP fluorescence in the nucleus are undergoing active silencing, and are analyzed for a growth phenotype. One-week old moss plants, regenerated from a single transformed protoplast, develop from polarized tip-growing protonemal cells. To quantify RNAi-induced phenotypes, plant area, a ...
... plants lacking GFP fluorescence in the nucleus are undergoing active silencing, and are analyzed for a growth phenotype. One-week old moss plants, regenerated from a single transformed protoplast, develop from polarized tip-growing protonemal cells. To quantify RNAi-induced phenotypes, plant area, a ...
Expression of the Nucleus-Encoded Chloroplast Division Genes and
... course of cell division and from generation to generation. The majority of algae (both unicellular and multicellular) have just one or at most only a few chloroplasts per cell, and it is obvious that a direct and precise relationship must exist between the host cell and chloroplast division. That is ...
... course of cell division and from generation to generation. The majority of algae (both unicellular and multicellular) have just one or at most only a few chloroplasts per cell, and it is obvious that a direct and precise relationship must exist between the host cell and chloroplast division. That is ...
The Life Cycle 12. - mt
... Ans. 1. The process of transfer of pollen grains from the anther to the stigma is called pollination. 2. If this transfer of pollen occurs in the same flower or another flower of the same plant, it is known as self pollination. 3. On the other hand, if pollen is transferred from one flower to the fl ...
... Ans. 1. The process of transfer of pollen grains from the anther to the stigma is called pollination. 2. If this transfer of pollen occurs in the same flower or another flower of the same plant, it is known as self pollination. 3. On the other hand, if pollen is transferred from one flower to the fl ...
Developmental origin and lineage plasticity of endogenous cardiac
... the epicardial layer. These cells emanate from a transitory progenitor structure appended to the primary heart tube called the proepicardium. Both the proepicardium and epicardium are marked by expression of the transcription factor Wilms tumor 1 (WT1). The proepicardium has its origins within the c ...
... the epicardial layer. These cells emanate from a transitory progenitor structure appended to the primary heart tube called the proepicardium. Both the proepicardium and epicardium are marked by expression of the transcription factor Wilms tumor 1 (WT1). The proepicardium has its origins within the c ...
Heat shock results in cell cycle delay and synchronisation of mitotic
... the early domains have already initiated mitosis. Such an embryo is shown in Fig. 5. The early domains, that have completed cycle 14, can be seen as areas of increased cell density (indicated by arrowheads). These cells and the surrounding ones that have not undertaken the 14th division are all in i ...
... the early domains have already initiated mitosis. Such an embryo is shown in Fig. 5. The early domains, that have completed cycle 14, can be seen as areas of increased cell density (indicated by arrowheads). These cells and the surrounding ones that have not undertaken the 14th division are all in i ...
Ccbe1 regulates Vegfc-mediated induction of Vegfr3
... from vegfr3 and ccbe1 mutants at the level of gross embryo morphology (supplementary material Fig. S1A-D), and that displayed a severe reduction in secondary angiogenesis and block in lymphatic precursor sprouting from the cardinal vein (Fig. 1A-D; Fig. 2A-H; supplementary material Fig. S1E-K). At 5 ...
... from vegfr3 and ccbe1 mutants at the level of gross embryo morphology (supplementary material Fig. S1A-D), and that displayed a severe reduction in secondary angiogenesis and block in lymphatic precursor sprouting from the cardinal vein (Fig. 1A-D; Fig. 2A-H; supplementary material Fig. S1E-K). At 5 ...
www .bio x.com Anti-VIRAL HAEMORRHAGIC SEPTICAEMIA
... thousand tons of trout are lost to this disease each year. Three distinct serotypes of this virus have been identified. The rainbow trout (Oncorhynchus mykiss) appears to be very sensitive to this virus. However, other Salmonidae, such as the brown trout (Salmo trutta fario L.), grayling (Thymallus ...
... thousand tons of trout are lost to this disease each year. Three distinct serotypes of this virus have been identified. The rainbow trout (Oncorhynchus mykiss) appears to be very sensitive to this virus. However, other Salmonidae, such as the brown trout (Salmo trutta fario L.), grayling (Thymallus ...
Regulated protein degradation controls PKA function and cell
... SP60 prespore-specific promoter. Expression from the ecmAO prestalk-specific promoter results in a mature fruiting body with an enlarged sorus containing mature spores and a shorter, thicker stalk than is observed in a wild-type fruiting body (compare fruiting bodies in Figs. 2C and 5A, open arrow; ...
... SP60 prespore-specific promoter. Expression from the ecmAO prestalk-specific promoter results in a mature fruiting body with an enlarged sorus containing mature spores and a shorter, thicker stalk than is observed in a wild-type fruiting body (compare fruiting bodies in Figs. 2C and 5A, open arrow; ...
Regulated Expression of Vimentin cDNA in Cells in the Presence
... (1986). 50 subelones were obtained and examined for human vimentin content by immunofluorescence microscopy. 25 of the subclones were found to be essentially vimentin negative, 22 were mosaics with a significant number of positive and negative cells, and 3 subclones were obtained that appeared to co ...
... (1986). 50 subelones were obtained and examined for human vimentin content by immunofluorescence microscopy. 25 of the subclones were found to be essentially vimentin negative, 22 were mosaics with a significant number of positive and negative cells, and 3 subclones were obtained that appeared to co ...
Plastid degeneration in Tillandsia (Bromeliaceae) provides
... cytoplasm of the tapetal cells tended to become more electron dense (Fig.s 2 and 3). In this stage many MLBs were observed entering a small vacuole (Fig. 2) or already included in it (Fig. 3). In this latter situation the MLB appeared to have a central area with a lower electron density but always c ...
... cytoplasm of the tapetal cells tended to become more electron dense (Fig.s 2 and 3). In this stage many MLBs were observed entering a small vacuole (Fig. 2) or already included in it (Fig. 3). In this latter situation the MLB appeared to have a central area with a lower electron density but always c ...
Targeting of Salmonella typhimurium to Vesicles Containing
... transport newly synthesized soluble enzymes containing M6P from the TGN to late endosomes via clathrin-coated vesicles (von Figura and Hasilik, 1986; Kornfeld, 1987, 1992; Kornfeld and MeUman, 1989). Many intracellular pathogens interact with the prelysosomal and lysosomal compartments of phagocytic ...
... transport newly synthesized soluble enzymes containing M6P from the TGN to late endosomes via clathrin-coated vesicles (von Figura and Hasilik, 1986; Kornfeld, 1987, 1992; Kornfeld and MeUman, 1989). Many intracellular pathogens interact with the prelysosomal and lysosomal compartments of phagocytic ...
as Adobe PDF - Edinburgh Research Explorer
... Mouse mutants that lack LHX2 expression show unusually high levels of proliferation in the ventral diencephalic floor, with concomitant failure of infundibular evagination (Zhao et al., 2010). In Fgf10 knockout mice, furthermore, the infundibulum similarly fails to evaginate fully and infundibular c ...
... Mouse mutants that lack LHX2 expression show unusually high levels of proliferation in the ventral diencephalic floor, with concomitant failure of infundibular evagination (Zhao et al., 2010). In Fgf10 knockout mice, furthermore, the infundibulum similarly fails to evaginate fully and infundibular c ...
Isolation of obligately alkaliphilic magnetotactic bacteria from
... question is whether and how many magnetotactic prokaryotic organisms have been isolated and deposited in culture collections but have never been recognized as magnetotactic for various reasons. This may be most applicable to the sulfate-reducing bacteria, as another magnetotactic sulfate-reducing ba ...
... question is whether and how many magnetotactic prokaryotic organisms have been isolated and deposited in culture collections but have never been recognized as magnetotactic for various reasons. This may be most applicable to the sulfate-reducing bacteria, as another magnetotactic sulfate-reducing ba ...
Marrow-Derived Facilitating Cells Transplantation: Role of Bone
... of CpG-stimulated and unstimulated FC were incubated with the mAb for CD86 (anti-CD86 PE-Cy5) or isotype control (Rat-IgG PE-Cy5) for 30 min at 4°C. The cells were washed twice with FACS buffer and then fixed with 2% paraformaldehyde in PBS. For analysis of FoxP3 protein, intracellular labeling was ...
... of CpG-stimulated and unstimulated FC were incubated with the mAb for CD86 (anti-CD86 PE-Cy5) or isotype control (Rat-IgG PE-Cy5) for 30 min at 4°C. The cells were washed twice with FACS buffer and then fixed with 2% paraformaldehyde in PBS. For analysis of FoxP3 protein, intracellular labeling was ...
Cell Communication and Signaling
... [41,42]. Mouse embryonic fibroblasts from Vav1/2-/- deficient were prepared as described recently [43]. These cells were grown on gelatine-coated culture dishes in DMEM containing 10% FCS, non-essential amino acids and sodium pyruvate [43]. Human embryonic intestinal epithelial cells (INT-407), obta ...
... [41,42]. Mouse embryonic fibroblasts from Vav1/2-/- deficient were prepared as described recently [43]. These cells were grown on gelatine-coated culture dishes in DMEM containing 10% FCS, non-essential amino acids and sodium pyruvate [43]. Human embryonic intestinal epithelial cells (INT-407), obta ...
Gene Section PTPRJ (protein tyrosine phosphatase, receptor type, J)
... DEP-1 is a candidate human homologue of the mouse colon cancer susceptibility locus SCC1. Germline allele-specific epigenetic silencing of the gene PTPRJ, encoding DEP-1, is described in earlyonset familial colorectal cancer, due to a 170-kb intragenic duplication affecting the 5' end of the phospha ...
... DEP-1 is a candidate human homologue of the mouse colon cancer susceptibility locus SCC1. Germline allele-specific epigenetic silencing of the gene PTPRJ, encoding DEP-1, is described in earlyonset familial colorectal cancer, due to a 170-kb intragenic duplication affecting the 5' end of the phospha ...
Significance of hydrolytic enzymes expressed during xylem
... conducting cells lacked secondary thickenings (Edwards 1986). As the protoplast of a living cell greatly impedes water conductance (Raven 1993), it can be assumed that those structures were dead empty cell corpses. Conduits that are supported by secondary cell wall thickenings, the characteristic of ...
... conducting cells lacked secondary thickenings (Edwards 1986). As the protoplast of a living cell greatly impedes water conductance (Raven 1993), it can be assumed that those structures were dead empty cell corpses. Conduits that are supported by secondary cell wall thickenings, the characteristic of ...
Correlation of Endothelial Cell Shape and Wall Shear Stress in a
... vessels (i.e., away from branch points) the endothelial cells are elongated and aligned with the axis of the vessel, which is presumed to be the direction of blood flow. In regions of branching, however, the endothelial cell pattern not only is altered but also appears to be indicative of a time-ave ...
... vessels (i.e., away from branch points) the endothelial cells are elongated and aligned with the axis of the vessel, which is presumed to be the direction of blood flow. In regions of branching, however, the endothelial cell pattern not only is altered but also appears to be indicative of a time-ave ...
Synthesis of a Viral Protein with Molecular
... dried onto Whatman No. 3MM chromatography paper (0.33 mm thick), and exposed to preflashed (15) Kodak XR-1 X-Omat R film at 70°by the procedure described by Bonner and Laskey (3). Development was performed in Kodak DX-80 developer. The films were then scanned in a densitometer. We did not quantitat ...
... dried onto Whatman No. 3MM chromatography paper (0.33 mm thick), and exposed to preflashed (15) Kodak XR-1 X-Omat R film at 70°by the procedure described by Bonner and Laskey (3). Development was performed in Kodak DX-80 developer. The films were then scanned in a densitometer. We did not quantitat ...
Serotonin synchronises convergent extension of
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.