Programmed Cell Death in Neurons
... their trophic support in vivo. Difficulties encountered in this experimental model are those that are common to other primary neuronal culture paradigms, including the inability to obtain a large number of cells from each animal and the lack of an efficient transfection method to ectopically express ...
... their trophic support in vivo. Difficulties encountered in this experimental model are those that are common to other primary neuronal culture paradigms, including the inability to obtain a large number of cells from each animal and the lack of an efficient transfection method to ectopically express ...
Modulation of T cell signaling by the actin cytoskeleton
... imaging at interfaces. When an excitation laser is totally reflected at the interface between glass and cell media, only fluorescent molecules located within ,200 nm of the interface are illuminated. TIRFM is a powerful technique to visualize single molecules in or near cell membranes (for details, ...
... imaging at interfaces. When an excitation laser is totally reflected at the interface between glass and cell media, only fluorescent molecules located within ,200 nm of the interface are illuminated. TIRFM is a powerful technique to visualize single molecules in or near cell membranes (for details, ...
Inhibition of the p53 tumor suppressor by MDM2
... (Fortunato, 2014); also, mutant p53 -/- along with an miRNA control group containing wild type p53 are also included. In order to obtain direct evidence of miRNA-1827 interaction with MDM2 in these cell lines, a Biotin-streptavidin systematic procedure will be performed. Streptavidin, a purified pro ...
... (Fortunato, 2014); also, mutant p53 -/- along with an miRNA control group containing wild type p53 are also included. In order to obtain direct evidence of miRNA-1827 interaction with MDM2 in these cell lines, a Biotin-streptavidin systematic procedure will be performed. Streptavidin, a purified pro ...
Growth and killing of a Salmonella enterica serovar
... the moment of their introduction into the cytosol, and therefore on the bacterial response. This second method has been applied to deliver S. typhimurium, Yersinia enterocolitica and non-pathogenic E. coli into the cytosol of epithelial cells, where none displayed any replication (Goetz et al., 2001 ...
... the moment of their introduction into the cytosol, and therefore on the bacterial response. This second method has been applied to deliver S. typhimurium, Yersinia enterocolitica and non-pathogenic E. coli into the cytosol of epithelial cells, where none displayed any replication (Goetz et al., 2001 ...
DELIVERY OF PROTEIN USING NANOPARTICLE Research Article A.ROBIN
... Nanomedicine, a branch of Nanotechnology involves the use of drugs and carriers to manipulate at the level of molecules and atoms. It’s a promising field of research – to locate, diagnose and treat disease1. It involves the use of particles at nano scale to carry the medicinal agents to identify and ...
... Nanomedicine, a branch of Nanotechnology involves the use of drugs and carriers to manipulate at the level of molecules and atoms. It’s a promising field of research – to locate, diagnose and treat disease1. It involves the use of particles at nano scale to carry the medicinal agents to identify and ...
Refraction effect in an in-plane-switching blue
... diameter and short coherence length, and (3) optically isotropic dark state. Since no surfactant is needed to generate uniform molecular alignment for PS-BPLC, the device fabrication process can be much simplified. And fast response time is particularly desirable because it not only reduces motion b ...
... diameter and short coherence length, and (3) optically isotropic dark state. Since no surfactant is needed to generate uniform molecular alignment for PS-BPLC, the device fabrication process can be much simplified. And fast response time is particularly desirable because it not only reduces motion b ...
PDF - ScienceAsia
... PHA (as determined by 1 H NMR spectroscopy). The residue was then re-dissolved into 7.00 ml. To each 1.00-ml aliquot was added 2 ml of various cold solvents (methanol, ethanol, propanol, isopropanol, ethyl acetate, hexane, or acetone) to re-precipitate to obtain a solid which then was filtered, drie ...
... PHA (as determined by 1 H NMR spectroscopy). The residue was then re-dissolved into 7.00 ml. To each 1.00-ml aliquot was added 2 ml of various cold solvents (methanol, ethanol, propanol, isopropanol, ethyl acetate, hexane, or acetone) to re-precipitate to obtain a solid which then was filtered, drie ...
PDF （576KB）
... Recently, small membrane vesicles, called exosomes (also known as extracellular vesicles), have been at the center of attention again. Current vigorous worldwide research found that exosomes play multiple important roles in physiological and pathological phenomena. In this review, we will summarize ...
... Recently, small membrane vesicles, called exosomes (also known as extracellular vesicles), have been at the center of attention again. Current vigorous worldwide research found that exosomes play multiple important roles in physiological and pathological phenomena. In this review, we will summarize ...
Viability and Cytotoxicity Assay Kits for Diverse Cell Types
... cell-mediated cytotoxicity by fluorescence microscopy and the viability of boar sperm by fluorescence microscopy. The LIVE/DEAD Viability/Cytotoxicity Kit is intended for use with animal cells that can be analyzed within about an hour of adding the dyes to the cells. The kit components, number of as ...
... cell-mediated cytotoxicity by fluorescence microscopy and the viability of boar sperm by fluorescence microscopy. The LIVE/DEAD Viability/Cytotoxicity Kit is intended for use with animal cells that can be analyzed within about an hour of adding the dyes to the cells. The kit components, number of as ...
Monomeric alpha-catenin links cadherin to the actin cytoskeleton
... Ridhdhi Desai1,3 , Ritu Sarpal1,3 , Noboru Ishiyama2 , Milena Pellikka1 , Mitsuhiko Ikura2 and Ulrich Tepass1,4 The linkage of adherens junctions to the actin cytoskeleton is essential for cell adhesion. The contribution of the cadherin–catenin complex to the interaction between actin and the adhere ...
... Ridhdhi Desai1,3 , Ritu Sarpal1,3 , Noboru Ishiyama2 , Milena Pellikka1 , Mitsuhiko Ikura2 and Ulrich Tepass1,4 The linkage of adherens junctions to the actin cytoskeleton is essential for cell adhesion. The contribution of the cadherin–catenin complex to the interaction between actin and the adhere ...
www.XtremePapers.com
... different variants of some question papers for our most popular assessments with large and widespread candidature. The question papers are closely related and the relationships between them have been thoroughly established using our assessment expertise. All versions of the paper give assessment of ...
... different variants of some question papers for our most popular assessments with large and widespread candidature. The question papers are closely related and the relationships between them have been thoroughly established using our assessment expertise. All versions of the paper give assessment of ...
ABSTRACT Title of Document:
... Embryonic stem cells (ESCs) are characterized by their ability to self-renew and capacity to give rise to a broad spectrum of differentiated cell types. Pluripotency is maintained during ESC self-renewal through the promotion of proliferation and the prevention of differentiation. ESCs can prolifera ...
... Embryonic stem cells (ESCs) are characterized by their ability to self-renew and capacity to give rise to a broad spectrum of differentiated cell types. Pluripotency is maintained during ESC self-renewal through the promotion of proliferation and the prevention of differentiation. ESCs can prolifera ...
SNARE complexes of different composition jointly
... followed by a C-terminal hydrophobic anchor (“tail anchor”) that mediates insertion into the endoplasmic reticulum (ER) membrane via the GET pathway (Denic, 2012). The α-helices can fold back on to the SNARE domain, which represents the closed, fusion-incompetent conformation of the syntaxin, or be ...
... followed by a C-terminal hydrophobic anchor (“tail anchor”) that mediates insertion into the endoplasmic reticulum (ER) membrane via the GET pathway (Denic, 2012). The α-helices can fold back on to the SNARE domain, which represents the closed, fusion-incompetent conformation of the syntaxin, or be ...
Using monoclonal antibodies to label living root hairs: a novel tool
... Similarly, mAbs have been important in mapping specific polymers in various plants and parts therein using light microscopy (LM)-based immunofluorescnce and transmission electron microscopy (TEM)-based immunogold labeling [22-24]. For virtually all of these studies, cells and tissues are typically f ...
... Similarly, mAbs have been important in mapping specific polymers in various plants and parts therein using light microscopy (LM)-based immunofluorescnce and transmission electron microscopy (TEM)-based immunogold labeling [22-24]. For virtually all of these studies, cells and tissues are typically f ...
Brucella Intracellular Replication Requires Trafficking Through the
... variety of mammals including humans (1). Brucellae are 678 www.traffic.dk ...
... variety of mammals including humans (1). Brucellae are 678 www.traffic.dk ...
RECQ5 helicase promotes resolution of conflicts between
... To determine which region of RECQ5 is required for its interaction with RNAPI, different C-terminally truncated variants of GFP-tagged RECQ5 were overexpressed in HEK293 cells and tested for RPA194 binding by IP (Fig. S1, B and C). The results showed that the interaction between RECQ5 and RPA194 wa ...
... To determine which region of RECQ5 is required for its interaction with RNAPI, different C-terminally truncated variants of GFP-tagged RECQ5 were overexpressed in HEK293 cells and tested for RPA194 binding by IP (Fig. S1, B and C). The results showed that the interaction between RECQ5 and RPA194 wa ...
The L5 epitope: an early marker for neural induction in the chick
... Hensen's node from a donor embryo. As controls, we used either: two other IgM-secreting hybridoma cell lines, whose antibodies recognise cell surface antigens that are also present at the same stages of development in all cells (4B5; Norris, 1989) or in subsets of cells (HNK1; Canning and Stern, 198 ...
... Hensen's node from a donor embryo. As controls, we used either: two other IgM-secreting hybridoma cell lines, whose antibodies recognise cell surface antigens that are also present at the same stages of development in all cells (4B5; Norris, 1989) or in subsets of cells (HNK1; Canning and Stern, 198 ...
pdf-Dokument - Universität Bonn
... the conservation of numerous proteins including the GAP proteins. Among all these proteins, only a few have been characterized in detail. Recent studies have shown that three ARFGAPs, called VAN3, OsAGAP, SCARFACE, may play an important role in vesicle transport from the plasma membrane to the endos ...
... the conservation of numerous proteins including the GAP proteins. Among all these proteins, only a few have been characterized in detail. Recent studies have shown that three ARFGAPs, called VAN3, OsAGAP, SCARFACE, may play an important role in vesicle transport from the plasma membrane to the endos ...
Retinoid signaling regulates primitive (yolk sac
... apparently not essential. RXR␣ knockout mice do not die until approximately embryonic day 15.5 from severe cardiac defect. It was determined that there is a transient defect in definitive hematopoiesis in the mutant mouse caused by a delay in fetal liver hematopoietic development.7 In contrast, cons ...
... apparently not essential. RXR␣ knockout mice do not die until approximately embryonic day 15.5 from severe cardiac defect. It was determined that there is a transient defect in definitive hematopoiesis in the mutant mouse caused by a delay in fetal liver hematopoietic development.7 In contrast, cons ...
Direct killing of Epstein-Barr virus (EBV)–infected B
... PBL- or SF-derived lymphocytes were maintained in RPMI 1640 supplemented with 10% pooled human serum, 1 mM L-glutamine, and 150 IU/mL recombinant interleukin 2 (IL-2). Positive selection for CD4⫹ PBMCs was performed by immunomagnetic sorting using an anti-CD4–specific monoclonal Ab (mAb) as previous ...
... PBL- or SF-derived lymphocytes were maintained in RPMI 1640 supplemented with 10% pooled human serum, 1 mM L-glutamine, and 150 IU/mL recombinant interleukin 2 (IL-2). Positive selection for CD4⫹ PBMCs was performed by immunomagnetic sorting using an anti-CD4–specific monoclonal Ab (mAb) as previous ...
Chapter 4 PowerPoint - Hillsborough Community College
... • Three characteristics make connective tissues different from other primary tissues: – All have common embryonic origin: all arise from mesenchyme tissue as their tissue of origin – Have varying degrees of vascularity (cartilage is avascular, bone is highly vascularized) – Cells are suspended/embed ...
... • Three characteristics make connective tissues different from other primary tissues: – All have common embryonic origin: all arise from mesenchyme tissue as their tissue of origin – Have varying degrees of vascularity (cartilage is avascular, bone is highly vascularized) – Cells are suspended/embed ...
siRNA Screen Identifies Trafficking Host Factors that Modulate
... Viral infection requires extensive subcellular trafficking, including cell entry, delivery of the ...
... Viral infection requires extensive subcellular trafficking, including cell entry, delivery of the ...
ATM and - Cancer Research
... phosphorylation, we examined foci formation in F02-98 (ATRm/m) cells in the presence and absence of LY294002. In all cases, a similar level of IR-induced H2AX foci relative to control cells was observed (Fig. 1C). Thus, we conclude that ATR does not significantly contribute to IR-induced H2AX foci f ...
... phosphorylation, we examined foci formation in F02-98 (ATRm/m) cells in the presence and absence of LY294002. In all cases, a similar level of IR-induced H2AX foci relative to control cells was observed (Fig. 1C). Thus, we conclude that ATR does not significantly contribute to IR-induced H2AX foci f ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.