Functional coupling of microtubules to membranes
... frequent changes of direction. The saltatory (‘stop–start’) motility could relate to the competing activity of opposing motors (i.e. a ‘tug-of-war’ between those motors generating force towards the minus or plus ends, respectively) (Soppina et al., 2009). Alternatively, there are examples where such ...
... frequent changes of direction. The saltatory (‘stop–start’) motility could relate to the competing activity of opposing motors (i.e. a ‘tug-of-war’ between those motors generating force towards the minus or plus ends, respectively) (Soppina et al., 2009). Alternatively, there are examples where such ...
PrimeFlow™ RNA Assay Technology Validation Paper
... PrimeFlow™ RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cells change over time or in response to stimulation. This novel assay uses fluorescent in situ hybridization (FISH) to enable simultaneous ...
... PrimeFlow™ RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cells change over time or in response to stimulation. This novel assay uses fluorescent in situ hybridization (FISH) to enable simultaneous ...
Intracellular calcium: friend or foe?
... outside, the cell is able to accumulate up to millimolar concentrations of the indicator inside. Though these fluors have now been used widely in many cells types their precise intracellular location has rarely been fully documented. Other complications can be ester hydrolysis, occurring extracellul ...
... outside, the cell is able to accumulate up to millimolar concentrations of the indicator inside. Though these fluors have now been used widely in many cells types their precise intracellular location has rarely been fully documented. Other complications can be ester hydrolysis, occurring extracellul ...
Adipocyte lipolysis links obesity to breast cancer growth: adipocyte
... Metabolic reprogramming is considered an emerging hallmark of cancer cells and has attracted significant renewed interest both from the perspective of understanding tumorigenesis and as a potential therapeutic target [1]. An important outcome of this metabolic shift is activation of pathways that ge ...
... Metabolic reprogramming is considered an emerging hallmark of cancer cells and has attracted significant renewed interest both from the perspective of understanding tumorigenesis and as a potential therapeutic target [1]. An important outcome of this metabolic shift is activation of pathways that ge ...
CELLS, ORGANS & THE HUMAN BODY SYSTEMS TEACHER
... Key Idea 1: Living things are both similar to and different from each other and from nonliving things. Introduction: Living things are similar to each other yet different from nonliving things. The cell is a basic unit of structure and function of living things (cell theory). For all living things, ...
... Key Idea 1: Living things are both similar to and different from each other and from nonliving things. Introduction: Living things are similar to each other yet different from nonliving things. The cell is a basic unit of structure and function of living things (cell theory). For all living things, ...
Chapter 7 Membrane Structure and Function
... 12) According to the fluid mosaic model of cell membranes, which of the following is a true statement about membrane phospholipids? A) They can move laterally along the plane of the membrane. B) They frequently flip-flop from one side of the membrane to the other. C) They occur in an uninterrupted b ...
... 12) According to the fluid mosaic model of cell membranes, which of the following is a true statement about membrane phospholipids? A) They can move laterally along the plane of the membrane. B) They frequently flip-flop from one side of the membrane to the other. C) They occur in an uninterrupted b ...
Review - Trade Science Inc
... mal conditions? (B) Is that the genes for hyaluronidase Streptococcal cells[8]. It has been estimated that for a are substantially regulated??? Such that it doesn’t de- group of 100 Streptococcus pyogenes strains only 23 grade its own HA under normal conditions or (C) Is are capable of producing the ...
... mal conditions? (B) Is that the genes for hyaluronidase Streptococcal cells[8]. It has been estimated that for a are substantially regulated??? Such that it doesn’t de- group of 100 Streptococcus pyogenes strains only 23 grade its own HA under normal conditions or (C) Is are capable of producing the ...
Intracellular Triggering of Fas Aggregation and Recruitment of
... death receptors was analyzed by immunofluorescence flow cytometry in 4 105 cells as described previously (12, 31) in a Becton Dickinson FACSCalibur™ flow cytometer using anti-Fas SM1/1 mAb (Bender MedSystems) and specific antibodies against TNFR1 and TRAIL-R2/DR5 (Qbiogene, Oncogene Research Produ ...
... death receptors was analyzed by immunofluorescence flow cytometry in 4 105 cells as described previously (12, 31) in a Becton Dickinson FACSCalibur™ flow cytometer using anti-Fas SM1/1 mAb (Bender MedSystems) and specific antibodies against TNFR1 and TRAIL-R2/DR5 (Qbiogene, Oncogene Research Produ ...
From signal to form: aspects of the cytoskeleton
... made with molecular genetics that will allow the unravelling of the signal transduction pathways in root hair morphogenesis leading to growth. This paper first discusses cytological phenomena involved in the process of polar growth, such as cytoplasmic polarity, cytoplasmic streaming and the organiz ...
... made with molecular genetics that will allow the unravelling of the signal transduction pathways in root hair morphogenesis leading to growth. This paper first discusses cytological phenomena involved in the process of polar growth, such as cytoplasmic polarity, cytoplasmic streaming and the organiz ...
Cell Wall, Cytoskeleton, and Cell Expansion in Higher Plants
... The plant cell wall is composed of cellulose, non-cellulosic wall polysaccharide polymers such as hemicellulose and pectin, and a small amount of protein. The architecture of the cell wall is required not only to be strong and rigid to provide the structural support for the plant, but also to be for ...
... The plant cell wall is composed of cellulose, non-cellulosic wall polysaccharide polymers such as hemicellulose and pectin, and a small amount of protein. The architecture of the cell wall is required not only to be strong and rigid to provide the structural support for the plant, but also to be for ...
TOR SIGNALLING IN BUGS, BRAIN AND BRAWN
... coupled to give rise to cells of a specific size. There are certain exceptions, however, in which one process occurs independently of the other. For example, division without growth occurs during early development when embryonic cells divide before commencing growth. Growth without division occurs i ...
... coupled to give rise to cells of a specific size. There are certain exceptions, however, in which one process occurs independently of the other. For example, division without growth occurs during early development when embryonic cells divide before commencing growth. Growth without division occurs i ...
Treadmilling by FtsZ filaments drives peptidoglycan
... the motions of the division-specific PG synthases and associated cytoskeletal polymers. Total internal reflection microscopy (TIRFM) of a functional mNeonGreen-FtsZ fusion expressed from the native locus (Fig. S3A-C) frequently revealed directional movements of FtsZ signal within newly assembled Z r ...
... the motions of the division-specific PG synthases and associated cytoskeletal polymers. Total internal reflection microscopy (TIRFM) of a functional mNeonGreen-FtsZ fusion expressed from the native locus (Fig. S3A-C) frequently revealed directional movements of FtsZ signal within newly assembled Z r ...
A Nanobody-Based System Using Fluorescent Proteins as Scaffolds
... Transgenic lines expressing driver genes such as transcription factors or site-specific recombinases in specific cell populations can then be used to control the expression of genes in responder cassettes. However, the utility of individual lines is limited by a transgene’s functional abilities; rep ...
... Transgenic lines expressing driver genes such as transcription factors or site-specific recombinases in specific cell populations can then be used to control the expression of genes in responder cassettes. However, the utility of individual lines is limited by a transgene’s functional abilities; rep ...
Tansley review - Professor Gero Steinberg
... A deeper understanding of the MT-based transport mechanisms that underlie polar growth and plant infection requires detailed knowledge of the cellular organization of the cytoskeleton. Intensive work along these lines in U. maydis has been undertaken. Unexpectedly, these studies reveal that molecula ...
... A deeper understanding of the MT-based transport mechanisms that underlie polar growth and plant infection requires detailed knowledge of the cellular organization of the cytoskeleton. Intensive work along these lines in U. maydis has been undertaken. Unexpectedly, these studies reveal that molecula ...
A feeling for the micro-organism: structure on a small scale. Biofilms
... & Monier, 2003). In this report, we will use the term ‘biofilm’, which has been defined as a structured community of microbial cells consisting of either a single species or multiple species, adherent to a surface or interface, and according to the canonical definition ‘encased in an extracellular m ...
... & Monier, 2003). In this report, we will use the term ‘biofilm’, which has been defined as a structured community of microbial cells consisting of either a single species or multiple species, adherent to a surface or interface, and according to the canonical definition ‘encased in an extracellular m ...
PDF - Oxford Academic - Oxford University Press
... least five parameters influence the final leaf size: (i) the number of cells recruited from the meristem to the primordium; (ii) the rate of cell division; (iii) the extent of cell proliferation; (iv) cell expansion; and (v) the duration of meristemoid division (Gonzalez et al., 2012). Through tradi ...
... least five parameters influence the final leaf size: (i) the number of cells recruited from the meristem to the primordium; (ii) the rate of cell division; (iii) the extent of cell proliferation; (iv) cell expansion; and (v) the duration of meristemoid division (Gonzalez et al., 2012). Through tradi ...
Identification and Characterization of Genes Required for
... 2007; Chera et al. 2009; Pellettieri et al. 2010). Thus, an important and unresolved question is whether there are genetic pathways that specifically regulate regeneration and compensatory proliferation. Alternatively, pathways that are required for growth under normal conditions may function in diff ...
... 2007; Chera et al. 2009; Pellettieri et al. 2010). Thus, an important and unresolved question is whether there are genetic pathways that specifically regulate regeneration and compensatory proliferation. Alternatively, pathways that are required for growth under normal conditions may function in diff ...
FEMS Microbiology Ecology 24
... following to 900 µl of bacterial suspension: 1 mg ml-1 of proteinase K (from Triterachium album); trypsin (type 1 from bovine pancreas); protease (type XIV from Streptomyces griseus); or chymotrypsin (type 1-S from bovine pancreas) (all from Sigma). These suspensions had a final concentration of 100 ...
... following to 900 µl of bacterial suspension: 1 mg ml-1 of proteinase K (from Triterachium album); trypsin (type 1 from bovine pancreas); protease (type XIV from Streptomyces griseus); or chymotrypsin (type 1-S from bovine pancreas) (all from Sigma). These suspensions had a final concentration of 100 ...
FREE Sample Here
... full file at http://testbankcorner.eu 33) Which of the following is most likely true of a protein that cotransports glucose and sodium ions into the intestinal cells of an animal? A) Sodium and glucose compete for the same binding site in the cotransporter. B) Glucose entering the cell down its con ...
... full file at http://testbankcorner.eu 33) Which of the following is most likely true of a protein that cotransports glucose and sodium ions into the intestinal cells of an animal? A) Sodium and glucose compete for the same binding site in the cotransporter. B) Glucose entering the cell down its con ...
384 The non-canonical Wnt ligand, Wnt4, is highly expressed in
... The non-canonical Wnt ligand, Wnt4, is highly expressed in pancreatic beta cells and its expression is negatively correlated with cell growth H.J. Welters, A. Henderson, A. Bowen; Institute of Biomedical and Clinical Science, University of Exeter Medical School, UK. Background and aims: We have prev ...
... The non-canonical Wnt ligand, Wnt4, is highly expressed in pancreatic beta cells and its expression is negatively correlated with cell growth H.J. Welters, A. Henderson, A. Bowen; Institute of Biomedical and Clinical Science, University of Exeter Medical School, UK. Background and aims: We have prev ...
Manuscrit en préparation (soumission prévue à Int - HAL
... amounts of omega-3 (n-3) fatty acids, display a lower breast cancer risk [1, 2]. This effect seems to be related to specific n-3 polyunsaturated fatty acids and/or to the ratio of n-6 to n-3 fatty acids [3-6]. Long-chain n-3 fatty acids found in fatty cold-water fish, particularly eicosapentaenoic a ...
... amounts of omega-3 (n-3) fatty acids, display a lower breast cancer risk [1, 2]. This effect seems to be related to specific n-3 polyunsaturated fatty acids and/or to the ratio of n-6 to n-3 fatty acids [3-6]. Long-chain n-3 fatty acids found in fatty cold-water fish, particularly eicosapentaenoic a ...
Anion homeostasis is important for non
... 2.1. Inhibition of anion channels decreases release of infectious BK The effect of DIDS on BKPyV release was tested in renal proximal tubule epithelial (RPTE) cells, a primary human cell line that supports efficient replication of BKPyV and the closest cell culture system to the natural site of infe ...
... 2.1. Inhibition of anion channels decreases release of infectious BK The effect of DIDS on BKPyV release was tested in renal proximal tubule epithelial (RPTE) cells, a primary human cell line that supports efficient replication of BKPyV and the closest cell culture system to the natural site of infe ...
OPTIMISATION OF CHO CELL GROWTH AND
... economic feasibility of these processes. Simultaneously with product yield, product authenticity is a crucial aspect to consider as it may per se affect the therapeutic value of such proteins. More defmed culture media are being developed, particularly to ensure batch product consistency. A Chinese ...
... economic feasibility of these processes. Simultaneously with product yield, product authenticity is a crucial aspect to consider as it may per se affect the therapeutic value of such proteins. More defmed culture media are being developed, particularly to ensure batch product consistency. A Chinese ...
A primer on the mouse basal body
... While it is unclear whether basal bodies of distinct types of cilia contain sets of proteins unique to that ciliary type, the structure of the cilium itself can vary in ways that may be dependent on the basal body [17]. One example is the transition zone, a region between the basal body and cilium c ...
... While it is unclear whether basal bodies of distinct types of cilia contain sets of proteins unique to that ciliary type, the structure of the cilium itself can vary in ways that may be dependent on the basal body [17]. One example is the transition zone, a region between the basal body and cilium c ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.