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Unprocessed Proinsulin Promotes Cell Survival During
... related to or independent of hyperglycemia, may contribute to the appearence of congenital abnormalities. Low insulin levels were reported to cause retarded growth and development in cultured rat embryos (9), but the inaccessibility of neurulating embryos in mammalian models limits in vivo studies. ...
... related to or independent of hyperglycemia, may contribute to the appearence of congenital abnormalities. Low insulin levels were reported to cause retarded growth and development in cultured rat embryos (9), but the inaccessibility of neurulating embryos in mammalian models limits in vivo studies. ...
Specification of the C. elegans MS blastomere by the T
... figure (Mello et al., 1992). The table summarizing the major embryonic cell types made by descendants of AB, MS, D and C was adapted from lineage data (Sulston et al., 1983). In embryo diagrams, anterior is towards the left, and dorsal is upwards. ...
... figure (Mello et al., 1992). The table summarizing the major embryonic cell types made by descendants of AB, MS, D and C was adapted from lineage data (Sulston et al., 1983). In embryo diagrams, anterior is towards the left, and dorsal is upwards. ...
Germ lineage specification from a pluripotent primitive ectoderm
... respond by forming any of the cell types found in the adult organism. Cell lines have been derived from human blastocysts that share many of the characteristics of mouse ES cells (Thomson, Itskovitz-Eldor et al. 1998). Although it is not possible to undertake chimera formation using human ES cells, ...
... respond by forming any of the cell types found in the adult organism. Cell lines have been derived from human blastocysts that share many of the characteristics of mouse ES cells (Thomson, Itskovitz-Eldor et al. 1998). Although it is not possible to undertake chimera formation using human ES cells, ...
Nucleolytic processing of aberrant replication intermediates by an
... the one-step polymerase chain reaction method (26). Growing conditions, synchronization and western blotting Yeast strains were grown in YPDA media at 28◦ C unless otherwise stated. For G1 arrests, early log phase cells were arrested with 4 g/ml ␣-Factor and then released in S phase by centrifugati ...
... the one-step polymerase chain reaction method (26). Growing conditions, synchronization and western blotting Yeast strains were grown in YPDA media at 28◦ C unless otherwise stated. For G1 arrests, early log phase cells were arrested with 4 g/ml ␣-Factor and then released in S phase by centrifugati ...
Full Text - The International Journal of Developmental Biology
... show a range of defects that include a loss of endodermal structures, and varying degrees of loss of targets of 4d inductive signaling. Veligers that result from injection of Ilyanassa Delta siRNAi in general mimic the defects observed in the DAPT treated larvae. The most severe DAPT phenotypes mimi ...
... show a range of defects that include a loss of endodermal structures, and varying degrees of loss of targets of 4d inductive signaling. Veligers that result from injection of Ilyanassa Delta siRNAi in general mimic the defects observed in the DAPT treated larvae. The most severe DAPT phenotypes mimi ...
Interactions between plant endomembrane systems and the actin
... physically connected to the ER surface in higher plants (Sparkes et al., 2009b); they interact with the ER through a proteinaceous bridge, which contains COPII proteins required for ER exit site (ERES) formation (Hanton et al., 2009). Interactions between microtubules and ERES components have been d ...
... physically connected to the ER surface in higher plants (Sparkes et al., 2009b); they interact with the ER through a proteinaceous bridge, which contains COPII proteins required for ER exit site (ERES) formation (Hanton et al., 2009). Interactions between microtubules and ERES components have been d ...
Essential roles of the Fas-Fas ligand pathway in the development of
... predominantly in activated T lymphocytes and natural killer cells (2). FasL is one of the major effector molecules of cytotoxic T lymphocytes such as CD8+ T cells and CD4+ T-helper 1–type T cells (3–5). In the immune system, Fas and FasL are involved in downregulation of immune reactions (6–8). Malf ...
... predominantly in activated T lymphocytes and natural killer cells (2). FasL is one of the major effector molecules of cytotoxic T lymphocytes such as CD8+ T cells and CD4+ T-helper 1–type T cells (3–5). In the immune system, Fas and FasL are involved in downregulation of immune reactions (6–8). Malf ...
A1980KJ36700001
... Mew Zealand in a small laboratory with very limited resources. There were no workers there with interests similar to my own for discussion of the literature. If there had been, a few publications mentioned in the review might have evoked more criticism than they did! "The King's English was always m ...
... Mew Zealand in a small laboratory with very limited resources. There were no workers there with interests similar to my own for discussion of the literature. If there had been, a few publications mentioned in the review might have evoked more criticism than they did! "The King's English was always m ...
Figure 6.1: Toluidine blue (TB) resin section illustrating the full
... presented with exocytosis of secretory granules (Fig. 6.7 B) or a total loss of cell apices (Figs. 6.9 A & B). More dark cells were present in the areas where secretion or recovery was seen to be taking place (Fig. 6.7 A), and in one gallbladder large lipid-like globules (Fig. 6.9 B) were seen in th ...
... presented with exocytosis of secretory granules (Fig. 6.7 B) or a total loss of cell apices (Figs. 6.9 A & B). More dark cells were present in the areas where secretion or recovery was seen to be taking place (Fig. 6.7 A), and in one gallbladder large lipid-like globules (Fig. 6.9 B) were seen in th ...
Nucleoredoxin and Wnt signaling in F9 cells
... Fortunately, researchers have been able to utilize the mouse F9 teratocarinoma cell line as a model to mimic this process in vitro [6, 7]. The F9 cell line was established by grafting 6-day old embryos into host testes of the mouse 129/Sv strain to produce teratomas [8]. Normally, these F9 teratocar ...
... Fortunately, researchers have been able to utilize the mouse F9 teratocarinoma cell line as a model to mimic this process in vitro [6, 7]. The F9 cell line was established by grafting 6-day old embryos into host testes of the mouse 129/Sv strain to produce teratomas [8]. Normally, these F9 teratocar ...
A proteomic chronology of gene expression through the cell cycle in
... redistribution provided that the original author and source are credited. ...
... redistribution provided that the original author and source are credited. ...
Mouse models of graft-versus-host disease: advances and limitations
... expressing receptors specific for that antigen. Cytokine storm: elevated levels of inflammatory mediators (such as TNF, IL-1 and IL-6) that potentiate further stimulation of immune effector cells such as T cells and macrophages in a positive-feedback loop; can occur in GvHD or in response to infect ...
... expressing receptors specific for that antigen. Cytokine storm: elevated levels of inflammatory mediators (such as TNF, IL-1 and IL-6) that potentiate further stimulation of immune effector cells such as T cells and macrophages in a positive-feedback loop; can occur in GvHD or in response to infect ...
Exploring the Potential of Whole Cell Biosensor
... integrated into Escherichia coli through DNA recombinant. Although it is good news to be able to develop such simple, rapid and portable method to determine the location for new gold mines, it is an irony that the mining processes caused more contaminations in the ground water and it requires extens ...
... integrated into Escherichia coli through DNA recombinant. Although it is good news to be able to develop such simple, rapid and portable method to determine the location for new gold mines, it is an irony that the mining processes caused more contaminations in the ground water and it requires extens ...
Microbiology with basis immunology_1
... A. an anaphylactic reaction B. degranulation and release of mediators C. a humoral immune response D. an IgG-complement mediated reaction E. a delayed hypersensitivity reaction ANSWER: E Monoclonal antibodies are produced by: A. lymphocytes B. myeloma cells. C. spleen cells. D. plasma cells. E. hybr ...
... A. an anaphylactic reaction B. degranulation and release of mediators C. a humoral immune response D. an IgG-complement mediated reaction E. a delayed hypersensitivity reaction ANSWER: E Monoclonal antibodies are produced by: A. lymphocytes B. myeloma cells. C. spleen cells. D. plasma cells. E. hybr ...
emboj200925-sup
... The ORF encoding the C-terminus of LtaS (residues 215-649) was cloned in pET11a (Invitrogen), and E. coli BL21 was transformed with the resultant plasmid pSG5918. Expression of the protein was induced by addition of 1mM IPTG final concentration to exponentially growing cells. The cells were harveste ...
... The ORF encoding the C-terminus of LtaS (residues 215-649) was cloned in pET11a (Invitrogen), and E. coli BL21 was transformed with the resultant plasmid pSG5918. Expression of the protein was induced by addition of 1mM IPTG final concentration to exponentially growing cells. The cells were harveste ...
PDF
... during and after sex determination. It is self-evident that an investigation of the ultrastructure of primordial germ cells, oogonia and very early oocytes is essential for a complete understanding of the events which lead to the production of the unfertilized egg. As far as is known, the only obser ...
... during and after sex determination. It is self-evident that an investigation of the ultrastructure of primordial germ cells, oogonia and very early oocytes is essential for a complete understanding of the events which lead to the production of the unfertilized egg. As far as is known, the only obser ...
Adenosine affects expression of membrane molecules, cytokine and
... the 20-100 mM or µM range have been detected in injured tissues (13-16). In addition, about 20% of the intracellular ATP content from eukariotic cells can be released by type III secretion machinery (17). Moreover, there is accumulating evidence that activated neutrophils release AMP in the 10 µM ra ...
... the 20-100 mM or µM range have been detected in injured tissues (13-16). In addition, about 20% of the intracellular ATP content from eukariotic cells can be released by type III secretion machinery (17). Moreover, there is accumulating evidence that activated neutrophils release AMP in the 10 µM ra ...
PAK1 Mediates Resistance to PI3 Kinase Inhibition in Lymphomas
... different oncogenic pathways frequently overlap among different lymphoma types (3,7). Activation of the phosphatidyl-inositol tri-phosphate pathway (PI3K) is known to be a key oncogenic event in a number of malignancies (8) and results in increased proliferation and cell survival (9). Multiple PI3K ...
... different oncogenic pathways frequently overlap among different lymphoma types (3,7). Activation of the phosphatidyl-inositol tri-phosphate pathway (PI3K) is known to be a key oncogenic event in a number of malignancies (8) and results in increased proliferation and cell survival (9). Multiple PI3K ...
Monoclonal antibodies - Federation of American Societies for
... causes an explosion in the number of cells producing the highest antigen-binding ability (antibody affinity). This could explain why antibodies to a specific toxin or microbe dramatically increase in the blood after the foreign invader enters the body, and within a short period of time evolve such t ...
... causes an explosion in the number of cells producing the highest antigen-binding ability (antibody affinity). This could explain why antibodies to a specific toxin or microbe dramatically increase in the blood after the foreign invader enters the body, and within a short period of time evolve such t ...
in pdf format
... significant reduction (p<0.001) was observed in the volume of uterine fluid when comparing the mares at pre-treatment to the mares at day 7 posttreatment. One hundred percent of the control mares still had a bacterial endometritis at day 7, whereas those mares treated with mycobacterial cell wall fo ...
... significant reduction (p<0.001) was observed in the volume of uterine fluid when comparing the mares at pre-treatment to the mares at day 7 posttreatment. One hundred percent of the control mares still had a bacterial endometritis at day 7, whereas those mares treated with mycobacterial cell wall fo ...
14 March 2017 ABI Response to FCA CP17/3: Proposed Handbook
... Service’s (FOS) jurisdiction The Consultation Paper proposes a rule in the FCA Dispute Resolution Complaints ("DISP") sourcebook, to bring offering and issuing of ILS within the scope of the FOS jurisdiction. If the FCA implements this proposal, persons claiming to be ‘eligible complainants’ should ...
... Service’s (FOS) jurisdiction The Consultation Paper proposes a rule in the FCA Dispute Resolution Complaints ("DISP") sourcebook, to bring offering and issuing of ILS within the scope of the FOS jurisdiction. If the FCA implements this proposal, persons claiming to be ‘eligible complainants’ should ...
The Arabidopsis repressor of light signaling SPA1
... showing that the direct substrate of the COP1/SPA complex, CONSTANS, also acts in the phloem. Taken together, our results highlight the importance of phloem vascular tissue in coordinating growth and development. Because the SPA1 protein itself is incapable of moving from cell to cell, we suggest th ...
... showing that the direct substrate of the COP1/SPA complex, CONSTANS, also acts in the phloem. Taken together, our results highlight the importance of phloem vascular tissue in coordinating growth and development. Because the SPA1 protein itself is incapable of moving from cell to cell, we suggest th ...
Glossary - Zoology
... Spawning - fertilisation (outside, is sexually) - formation of blastula (with cilia inside) - gastrulation - free swimming planula larvae - settlement - stationary polyp - differentiation into a stack of polyps (asexually formed colony, strobolisation) - each saucer forms tentacles and seperates to ...
... Spawning - fertilisation (outside, is sexually) - formation of blastula (with cilia inside) - gastrulation - free swimming planula larvae - settlement - stationary polyp - differentiation into a stack of polyps (asexually formed colony, strobolisation) - each saucer forms tentacles and seperates to ...
in Murine Mast Cells by Recombinant Stem Cell Factor
... to cellular membranes in response to nanomolar calcium concentrations (21-24). There also exists a family of low molecular weight PLA2 (- 14 kD in size, called group II PLA2) that is released from cells during activation (25-28). Group II PLA2 are present in high quantities in inflammatory sites suc ...
... to cellular membranes in response to nanomolar calcium concentrations (21-24). There also exists a family of low molecular weight PLA2 (- 14 kD in size, called group II PLA2) that is released from cells during activation (25-28). Group II PLA2 are present in high quantities in inflammatory sites suc ...
Cell culture
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Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.