Exploring glycoside hydrolase family 5 (GH5) enzymes
... commercial processes. For example, the durable and long-lasting wood with high percentage of lignins is a very suitable building material, while low lignified materials are ideally utilized in paper production and in biomass saccharification for producing ethanol [35]. ...
... commercial processes. For example, the durable and long-lasting wood with high percentage of lignins is a very suitable building material, while low lignified materials are ideally utilized in paper production and in biomass saccharification for producing ethanol [35]. ...
7 Notes (Kingdom Fungi).
... plants. Endophytes sometimes produce toxins which discourage herbivores and insects from eating the leaves. Fungi-Animal Mutualism: Many animals and insects that eat either grass or wood (ie-cows and termites) have symbiotic fungi living in their guts (usually Chytrids) that produce cellulase enzyme ...
... plants. Endophytes sometimes produce toxins which discourage herbivores and insects from eating the leaves. Fungi-Animal Mutualism: Many animals and insects that eat either grass or wood (ie-cows and termites) have symbiotic fungi living in their guts (usually Chytrids) that produce cellulase enzyme ...
How and why does β-actin mRNA target?
... apical structures. The contribution of local translation has not been evaluated because the translation of targeted mRNA has not been observed in vivo. Thus, the timing and location of translation relative to the appearance of β-actin in apical structures has not been determined. However, the mechan ...
... apical structures. The contribution of local translation has not been evaluated because the translation of targeted mRNA has not been observed in vivo. Thus, the timing and location of translation relative to the appearance of β-actin in apical structures has not been determined. However, the mechan ...
- Wiley Online Library
... Isolated GCs and SCs were spherical, with similar size (35–40 lm in diameter) but different cytological features (Figure 1e,f). Most GN appeared to be positioned in the middle of the cell, and SN were close to the cell membrane (Figure 1e,f). In addition, the cytoplasm appearance differed (Zhao et a ...
... Isolated GCs and SCs were spherical, with similar size (35–40 lm in diameter) but different cytological features (Figure 1e,f). Most GN appeared to be positioned in the middle of the cell, and SN were close to the cell membrane (Figure 1e,f). In addition, the cytoplasm appearance differed (Zhao et a ...
Monitoring Growth of Beer Brewing Strains of
... monitors the incubation temperature and provides heat from multiple points within the read chamber, providing uniform temperatures across the plate. An often overlooked point is evaporation and the need for plate sealers. Long kinetic assays such as these require a better means of evaporation preven ...
... monitors the incubation temperature and provides heat from multiple points within the read chamber, providing uniform temperatures across the plate. An often overlooked point is evaporation and the need for plate sealers. Long kinetic assays such as these require a better means of evaporation preven ...
Selenium incorporation using recombinant techniques
... preparation. In addition, it has recently been reported that the use of auto-inducing media with a non-auxotrophic strain is also a viable system for producing SeMet-labelled proteins (Studier, 2005). This system entails the transformation of cells and then culturing them in a smaller volume for gre ...
... preparation. In addition, it has recently been reported that the use of auto-inducing media with a non-auxotrophic strain is also a viable system for producing SeMet-labelled proteins (Studier, 2005). This system entails the transformation of cells and then culturing them in a smaller volume for gre ...
ANALYSIS OF NF-κB, CASPASE-3, MMP-2, MMP
... (n=9) patients. There were 27 (67.5%) male and 13 (32.5%) female patients. There was a significant association between histological type and age groups. MCCHL type was significantly higher in children (80%), older adults (92.3%) and older age groups (100%), while NSCHL was higher in young adult grou ...
... (n=9) patients. There were 27 (67.5%) male and 13 (32.5%) female patients. There was a significant association between histological type and age groups. MCCHL type was significantly higher in children (80%), older adults (92.3%) and older age groups (100%), while NSCHL was higher in young adult grou ...
starry night regulates tissue polarity - Development
... 5422 J. Chae and others function of the protocadherins. Results of previous cell biological experiments suggest that protocadherins only weakly promote cell adhesion. The most interesting genetic data comes from the analysis of the dachsous (ds) and ft genes of Drosophila, which have recently been ...
... 5422 J. Chae and others function of the protocadherins. Results of previous cell biological experiments suggest that protocadherins only weakly promote cell adhesion. The most interesting genetic data comes from the analysis of the dachsous (ds) and ft genes of Drosophila, which have recently been ...
838 Woodend Road, Stratford, Connecticut 06615 PAGE 1
... In ancient times, the science of transformation was called “Alchima.” “Alchima” is the stream of processes through which matter can be elevated from one state to another. One stage of the alchemic process, called “Nigredo,” is linked with fermentation and is key to the transformation of life force w ...
... In ancient times, the science of transformation was called “Alchima.” “Alchima” is the stream of processes through which matter can be elevated from one state to another. One stage of the alchemic process, called “Nigredo,” is linked with fermentation and is key to the transformation of life force w ...
Structure-Function Relationships of Stem Cell Factor
... Western blot analysis. The presence and relative level of specific recombinant SCF proteins were determined by Western blotting, as described previo~sly.'~Aliquots of conditioned medium from appropriate cell lines were denatured, size-fractionated by NaDodS04/10% polyacrylamide gel electrophoresis, ...
... Western blot analysis. The presence and relative level of specific recombinant SCF proteins were determined by Western blotting, as described previo~sly.'~Aliquots of conditioned medium from appropriate cell lines were denatured, size-fractionated by NaDodS04/10% polyacrylamide gel electrophoresis, ...
Extracellular ATP Functions as an
... ATP is a ubiquitous, energy-rich compound in all cells of living organisms. It is found both within organelles, such as mitochondria and chloroplasts, and in the cytoplasm of higher organisms. The energy derived from ATP is used to drive a myriad of vital biochemical reactions that are fundamental t ...
... ATP is a ubiquitous, energy-rich compound in all cells of living organisms. It is found both within organelles, such as mitochondria and chloroplasts, and in the cytoplasm of higher organisms. The energy derived from ATP is used to drive a myriad of vital biochemical reactions that are fundamental t ...
Eph/ephrins and N-cadherin coordinate to control the
... Embryos electroporated with EGFP at HH St. 10 (previously described) were used for these experiments. EGFP-labeled embryos at E3 were surveyed using a fluorescence dissecting microscope (Carl Zeiss, Stemi SV11). EphB2-Fc (100 g/ml), ephrinB1-Fc (100 g/ml), and Fc-control (100 g/ml) fusion protein ...
... Embryos electroporated with EGFP at HH St. 10 (previously described) were used for these experiments. EGFP-labeled embryos at E3 were surveyed using a fluorescence dissecting microscope (Carl Zeiss, Stemi SV11). EphB2-Fc (100 g/ml), ephrinB1-Fc (100 g/ml), and Fc-control (100 g/ml) fusion protein ...
Melanocytes and melanogenesis - Our Dermatology Online journal
... in melanin content. It also increases levels of PAR 2 (Protease activated receptor) in keratinocytes which increases uptake and distribution of melanosomes by keratinocytes in the epidermis. It also increases Alpha-MSH, endothelin and ACTH which upregulate MC-1R, thereby enhancing melanocytes respon ...
... in melanin content. It also increases levels of PAR 2 (Protease activated receptor) in keratinocytes which increases uptake and distribution of melanosomes by keratinocytes in the epidermis. It also increases Alpha-MSH, endothelin and ACTH which upregulate MC-1R, thereby enhancing melanocytes respon ...
Indirect ELISA
... • Serum or some other sample containing primary antibody (Ab1) is added to an antigen-coated microtiter well and allowed to react with the antigen attached to the well. After any free Ab1 is washed away, the presence of antibody bound to the antigen is detected by adding an enzyme-conjugated seconda ...
... • Serum or some other sample containing primary antibody (Ab1) is added to an antigen-coated microtiter well and allowed to react with the antigen attached to the well. After any free Ab1 is washed away, the presence of antibody bound to the antigen is detected by adding an enzyme-conjugated seconda ...
BIO205 - National Open University of Nigeria
... The idea about cell structure expatiates that the membranes are fluid, with components that move, change, and perform vital physiological roles as they allow cells to communicate with each other and their environment. The course introduces you to the cell as the fundamental unit of life. The Cell Cy ...
... The idea about cell structure expatiates that the membranes are fluid, with components that move, change, and perform vital physiological roles as they allow cells to communicate with each other and their environment. The course introduces you to the cell as the fundamental unit of life. The Cell Cy ...
Journal of Bacteriology
... 169:4294-4301, 1987). In contrast to the attachment of carbon-limited cells, accumulation of manganeselimited rhizobia (cap formation) was already in full progress after 10 min of incubation; significantly delayed by 3-0-methyl-D-glucose, a pea lectin haptenic monosaccharide; partially resistant to ...
... 169:4294-4301, 1987). In contrast to the attachment of carbon-limited cells, accumulation of manganeselimited rhizobia (cap formation) was already in full progress after 10 min of incubation; significantly delayed by 3-0-methyl-D-glucose, a pea lectin haptenic monosaccharide; partially resistant to ...
Production of Antimicrobial Metabolites by Bacillus subtilis
... Production of Antimicrobial Metabolites by Bacillus subtilis Immobilized in Polyacrylamide Gel M. Awais*, A. Pervez, Asim Yaqub and M.M. Shah Life Sciences Services (MA, AP, AY), and Biotechnology Programme (MMS), Department of Environmental Sciences, COMSATS Institute of Information Technology, Abb ...
... Production of Antimicrobial Metabolites by Bacillus subtilis Immobilized in Polyacrylamide Gel M. Awais*, A. Pervez, Asim Yaqub and M.M. Shah Life Sciences Services (MA, AP, AY), and Biotechnology Programme (MMS), Department of Environmental Sciences, COMSATS Institute of Information Technology, Abb ...
Remodeling of endosomes during lysosome biogenesis involves
... To determine the effect of rab5(Q79L) on endosome morphology, 10 mg/ml HRP (Sigma) was internalized in transfected and control cells for 30 minutes. Cells were then fixed in 1% glutaraldehyde. HRP was revealed by a DAB (Sigma, St Louis, MO) reaction as described previously (Desjardins et al., 1994). ...
... To determine the effect of rab5(Q79L) on endosome morphology, 10 mg/ml HRP (Sigma) was internalized in transfected and control cells for 30 minutes. Cells were then fixed in 1% glutaraldehyde. HRP was revealed by a DAB (Sigma, St Louis, MO) reaction as described previously (Desjardins et al., 1994). ...
"Allosteric Activation of Kinases: Design and Application of RapR
... kinases enable targeted control, but do not provide temporal control. Application of pharmacological inhibitors allows excellent temporal control, but is often not sufficiently specific. An elegant method developed by Shokat overcomes the problem of kinase inhibitor specificity by utilizing mutant k ...
... kinases enable targeted control, but do not provide temporal control. Application of pharmacological inhibitors allows excellent temporal control, but is often not sufficiently specific. An elegant method developed by Shokat overcomes the problem of kinase inhibitor specificity by utilizing mutant k ...
Expanded GAA repeats impair FXN gene expression and reposition
... Figure 1. The expanded GAA repeat FXN transgene associates with the NL more frequently in an FXN-GAA-MS2-Luc cell model. (A) Schematic representation of the pBACFXN-MS2-Luc and pBAC-FXN-GAA-MS2-Luc vectors. Each vector carries either six or ∼310 GAA repeats in intron 1, an array of 24 MBS in exon 2 ...
... Figure 1. The expanded GAA repeat FXN transgene associates with the NL more frequently in an FXN-GAA-MS2-Luc cell model. (A) Schematic representation of the pBACFXN-MS2-Luc and pBAC-FXN-GAA-MS2-Luc vectors. Each vector carries either six or ∼310 GAA repeats in intron 1, an array of 24 MBS in exon 2 ...
Amphibian gastrulation: history and evolution of a 125 year
... was to be investigated for decades. Mesoderm induction at a pregastrula stage was characterized later (Nieuwkoop, 1969). At the present time, an immense number of articles have dealt with cell inductive interactions in the Amphibian gastrula, a topic that has been renewed during the last decade by t ...
... was to be investigated for decades. Mesoderm induction at a pregastrula stage was characterized later (Nieuwkoop, 1969). At the present time, an immense number of articles have dealt with cell inductive interactions in the Amphibian gastrula, a topic that has been renewed during the last decade by t ...
Tissue Lab Practical Review
... Tissue Lab Practical Review For Mrs. Nemanic’s Stellar Students! ...
... Tissue Lab Practical Review For Mrs. Nemanic’s Stellar Students! ...
Homeostasis and the Importance for a Balance
... phosphoinositide-dependent kinase-1 (PDK1), and Ser473 is phosphorylated by mTORC2. AKT regulates several cellular processes such as survival and cell proliferation through a variety of downstream proteins, including BAD (Bcl-2-antagonist of cell death), FOXO (Forkhead Box O), NFB, and GSK-3 (glyc ...
... phosphoinositide-dependent kinase-1 (PDK1), and Ser473 is phosphorylated by mTORC2. AKT regulates several cellular processes such as survival and cell proliferation through a variety of downstream proteins, including BAD (Bcl-2-antagonist of cell death), FOXO (Forkhead Box O), NFB, and GSK-3 (glyc ...
Involvement of antimicrobial peptides in mussel defence
... Antimicrobial peptides are one of the innate immunity actors which were conserved along evolution. Last decade, they were purified from plants (Broekaert et al., 1995), invertebrates (Iwanaga et al., 1998; Bulet et al., 1999) and vertebrates (Lehrer and Ganz, 1999) and are suspected to be part of im ...
... Antimicrobial peptides are one of the innate immunity actors which were conserved along evolution. Last decade, they were purified from plants (Broekaert et al., 1995), invertebrates (Iwanaga et al., 1998; Bulet et al., 1999) and vertebrates (Lehrer and Ganz, 1999) and are suspected to be part of im ...
Antibodies in plantscience
... Primary screening of antibodies. Measurement of antigens; usual to ...
... Primary screening of antibodies. Measurement of antigens; usual to ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.