Caveolae as potential macromolecule trafficking
... structure which affords spatial continuity with the extracellular environment (Fig. 1a). At least in endothelial cells caveolae-like vesicles may also be observed as fused lines or clusters of vesicles at the plasmalemma [7]. The term ‘‘smooth coated’’ or ‘‘non-coated’’ vesicles has long been used t ...
... structure which affords spatial continuity with the extracellular environment (Fig. 1a). At least in endothelial cells caveolae-like vesicles may also be observed as fused lines or clusters of vesicles at the plasmalemma [7]. The term ‘‘smooth coated’’ or ‘‘non-coated’’ vesicles has long been used t ...
Dependence of an Adenosine-Activated Potassium Current on a
... intracellular GTP. Our resultssuggestthat the adenosinereceptor acts on a potassiumchannel through a G-protein and that adenylate cyclase modulation is not critically involved in this effect. A presentationof this result hasappearedin abstract form (Trussell and Jackson, 1986). Materials and Methods ...
... intracellular GTP. Our resultssuggestthat the adenosinereceptor acts on a potassiumchannel through a G-protein and that adenylate cyclase modulation is not critically involved in this effect. A presentationof this result hasappearedin abstract form (Trussell and Jackson, 1986). Materials and Methods ...
7th Grade Science - Pflugerville ISD
... • Most things, like water and oxygen, can diffuse directly through the lipid (fat) cell membrane because they are so small. • Some things are too large (sugars) to squeeze through the cell membrane. • The things that are too big to go through the membrane use passive transport, active transport, end ...
... • Most things, like water and oxygen, can diffuse directly through the lipid (fat) cell membrane because they are so small. • Some things are too large (sugars) to squeeze through the cell membrane. • The things that are too big to go through the membrane use passive transport, active transport, end ...
Binding Sites for Bacterial Flagella at the Surface of the Soil Amoeba
... numbers of motile bacteria at its periphery. These agglutinated bacteria were endocytosed after being swept backwards into what she termed a ‘cap’. The wider significance of this finding was not appreciated, and it is ironic that it was 20 years later that what is generally considered to be the firs ...
... numbers of motile bacteria at its periphery. These agglutinated bacteria were endocytosed after being swept backwards into what she termed a ‘cap’. The wider significance of this finding was not appreciated, and it is ironic that it was 20 years later that what is generally considered to be the firs ...
Evidence for the presence of calsequestrin in two structurally
... field as shown in Fig. 2, d and g, respectively. Specific fluorescent staining in transverse sections was present throughout the cytoplasmic region of the myofibers and consisted of small punctate foci (Fig. 2, a and c). Although the fluorescent foci were present at the cell periphery they did not a ...
... field as shown in Fig. 2, d and g, respectively. Specific fluorescent staining in transverse sections was present throughout the cytoplasmic region of the myofibers and consisted of small punctate foci (Fig. 2, a and c). Although the fluorescent foci were present at the cell periphery they did not a ...
Epithelial enhancement of connective tissue
... In chick embryos, epithelial somites are formed from the segmental plate mesoderm in an anterior to posterior fashion (Williams, 1910). The epithelial somite subsequently differentiates into three identifiable regions, each of which will give rise to a specific type of connective tissue. The cells i ...
... In chick embryos, epithelial somites are formed from the segmental plate mesoderm in an anterior to posterior fashion (Williams, 1910). The epithelial somite subsequently differentiates into three identifiable regions, each of which will give rise to a specific type of connective tissue. The cells i ...
Quantitative analysis of changes in spatial distribution and plus
... daughter nuclei (n). The growing cell plate (cp) is surrounded by many vesicles and is sandwiched between the two sets of microtubules. (D) Transitionalphragmoplast stage (2.8⫻2.8⫻0.8 m), which lacks a cell-plate assembly matrix and displays many short microtubules on both sides of the maturing cel ...
... daughter nuclei (n). The growing cell plate (cp) is surrounded by many vesicles and is sandwiched between the two sets of microtubules. (D) Transitionalphragmoplast stage (2.8⫻2.8⫻0.8 m), which lacks a cell-plate assembly matrix and displays many short microtubules on both sides of the maturing cel ...
Monolignol glucosides as intermediate compounds in
... synthesis of coniferin and a slow turnover of this compound (Marcinowski and Grisebach 1977). The concentration of coniferin in differentiating xylem of Japanese black pine (Pinus thunbergii Parl.) is highest at the beginning of May, before the most active lignifying season, June and July, and there ...
... synthesis of coniferin and a slow turnover of this compound (Marcinowski and Grisebach 1977). The concentration of coniferin in differentiating xylem of Japanese black pine (Pinus thunbergii Parl.) is highest at the beginning of May, before the most active lignifying season, June and July, and there ...
Endothelial Cells Exposed To Fluid Shear Stress
... Intimal hyperplasia, a typical feature of atherosclerosis, is found to occur in response to hemodynamic conditions such as shear stress. In the hyperplastic lesion, it is well known that smooth muscle cells (SMCs) migrate from media to intima. Migration of SMCs may be associated with endothelial cel ...
... Intimal hyperplasia, a typical feature of atherosclerosis, is found to occur in response to hemodynamic conditions such as shear stress. In the hyperplastic lesion, it is well known that smooth muscle cells (SMCs) migrate from media to intima. Migration of SMCs may be associated with endothelial cel ...
The Relation Between Scale and the Completeness of Pattern in
... 1) How precise is the maintenance of the experimentally by creating two different proportions between pattern parts, in sets of abnormal conditions. terms of the relative space or cell numbers In the first test, so many presumptively devoted to them, under development at ventral cells (—those furthe ...
... 1) How precise is the maintenance of the experimentally by creating two different proportions between pattern parts, in sets of abnormal conditions. terms of the relative space or cell numbers In the first test, so many presumptively devoted to them, under development at ventral cells (—those furthe ...
Alveolar macrophages are the main source for tumour necrosis H. Fehrenbach
... Bronchoscopy and BAL were performed as described previously [8, 19]. A volume of 200–300 mL of sterile saline (0.9% NaCl) was instilled into a lingula or middle-lobe segment in 25 mL aliquots. Each aliquot was immediately aspirated. Recovery was (mean¡SEM) 68.9¡9.9% in the nonsarcoidosis group versu ...
... Bronchoscopy and BAL were performed as described previously [8, 19]. A volume of 200–300 mL of sterile saline (0.9% NaCl) was instilled into a lingula or middle-lobe segment in 25 mL aliquots. Each aliquot was immediately aspirated. Recovery was (mean¡SEM) 68.9¡9.9% in the nonsarcoidosis group versu ...
Biology EOCEP Review - Teacher Copy
... Laurens District 55 High School – 2010 Answer If plants are exposed to 2, 4, 6, 8, 10 and 12 hours of light a day then the plants that are exposed to 10 hours will grow the most. ...
... Laurens District 55 High School – 2010 Answer If plants are exposed to 2, 4, 6, 8, 10 and 12 hours of light a day then the plants that are exposed to 10 hours will grow the most. ...
Cytoplasmic Actin in Neuronal Processes as a Possible Mediator of
... signals to neurons that were modified by various experimental treatments may change the physicochemical properties of the cytoplasm which may induce the assembly or disassembly of the actin lattice with a consequent contraction or relaxation of the element involved. The dentate fascia of the hippoca ...
... signals to neurons that were modified by various experimental treatments may change the physicochemical properties of the cytoplasm which may induce the assembly or disassembly of the actin lattice with a consequent contraction or relaxation of the element involved. The dentate fascia of the hippoca ...
PDF
... containing lmgml" 1 of collagenase (Type 1; Sigma Ltd) for 10-15 min. This facilitated dissection of specific somites or other tissues which in most cases were transferred to a Ca2+/Mg2+-free Steinberg's solution containing 0-4mM-EDTA (Sigma) for 20-30 min. The dissociated tissues then were drawn up ...
... containing lmgml" 1 of collagenase (Type 1; Sigma Ltd) for 10-15 min. This facilitated dissection of specific somites or other tissues which in most cases were transferred to a Ca2+/Mg2+-free Steinberg's solution containing 0-4mM-EDTA (Sigma) for 20-30 min. The dissociated tissues then were drawn up ...
The Assimilation of Amino-acids by Bacteria
... Investigation of nutrition of organisms. The nitrogen requirements of staphylococci were investigated by Gladstone (1937); his methods were followed in this work. I n general, a complete nutrient medium consisting of salts, nicotinamide, aneurin and pure amino-acids (Gladstone, 1937) was prepared an ...
... Investigation of nutrition of organisms. The nitrogen requirements of staphylococci were investigated by Gladstone (1937); his methods were followed in this work. I n general, a complete nutrient medium consisting of salts, nicotinamide, aneurin and pure amino-acids (Gladstone, 1937) was prepared an ...
Sample pages 1 PDF
... tion of tissues. Hence, it is always recommended that a fresh formaldehyde solution is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldeh ...
... tion of tissues. Hence, it is always recommended that a fresh formaldehyde solution is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldeh ...
Craniofacial Development and the Evolution of the Vertebrates: the
... modern treatment of the issue). However, this was never truly integrated with the transcendental morphology until recently, when molecular developmental biology became the glue to unite them (see de Beer, 1926, 1937, 1958; Jarvik, 1980; Couly et al., 1993, 1998; see Hanken and Hall, 1993, for studie ...
... modern treatment of the issue). However, this was never truly integrated with the transcendental morphology until recently, when molecular developmental biology became the glue to unite them (see de Beer, 1926, 1937, 1958; Jarvik, 1980; Couly et al., 1993, 1998; see Hanken and Hall, 1993, for studie ...
ARVO 2017 Annual Meeting Abstracts 106 Development of the
... UAS-CkaRNAi; UAS-StripRNAi; UAS-twsRNAi; UAS-PP2A-B’RNAi; UAS-wdbRNAi; overexpression transgenes: UAS-Hpo, UAS-DIAP1, UAS-CycE. cLOF was induced via ey-FLP, whereas UAS expression was driven by the eye-disc specific constitutive ey-FLP act>interruption cassette>GAL4 driver. Tissue was assessed by IH ...
... UAS-CkaRNAi; UAS-StripRNAi; UAS-twsRNAi; UAS-PP2A-B’RNAi; UAS-wdbRNAi; overexpression transgenes: UAS-Hpo, UAS-DIAP1, UAS-CycE. cLOF was induced via ey-FLP, whereas UAS expression was driven by the eye-disc specific constitutive ey-FLP act>interruption cassette>GAL4 driver. Tissue was assessed by IH ...
Molecular Microbiology
... with similar retention times were submitted to mass spectrometric analysis, amino acid analysis and NMR analysis. Mass spectrometric analysis of the lipopeptides Diode array UV of the HPLC fractions showed no evidence for the presence of aromatic amino acids (that absorb at 254 nm). Electrospray mas ...
... with similar retention times were submitted to mass spectrometric analysis, amino acid analysis and NMR analysis. Mass spectrometric analysis of the lipopeptides Diode array UV of the HPLC fractions showed no evidence for the presence of aromatic amino acids (that absorb at 254 nm). Electrospray mas ...
Plant Physiology
... the Fahraeus slide assemblies were examined under the light microscope at daily intervals for 4 d. Roots were examined every 4 to 6 h during the first day of culture in the Fahraeus slide assemblies. The total root system of the seedling was examined during the experiments. Photographs were taken of ...
... the Fahraeus slide assemblies were examined under the light microscope at daily intervals for 4 d. Roots were examined every 4 to 6 h during the first day of culture in the Fahraeus slide assemblies. The total root system of the seedling was examined during the experiments. Photographs were taken of ...
Mesoderm migration in Drosophila is a multi-step process
... molecules that could contribute to specific steps during mesoderm migration to test the hypothesis that mesoderm migration has temporally distinct inputs. We chose to examine the small GTPase Rap1 and integrins, as both have been implicated in migration and linked to FGF signaling (Mori et al., 2008 ...
... molecules that could contribute to specific steps during mesoderm migration to test the hypothesis that mesoderm migration has temporally distinct inputs. We chose to examine the small GTPase Rap1 and integrins, as both have been implicated in migration and linked to FGF signaling (Mori et al., 2008 ...
Plant Gravitropism. Unraveling the Ups and
... plasma membrane. Network disruption would modify the tension of actin filaments within the statocytes, thereby activating mechanosensitive ion channels at the plasma membrane (Yoder et al., 2001). In turn, channel activation could be responsible, directly or indirectly, for the rapid transients in C ...
... plasma membrane. Network disruption would modify the tension of actin filaments within the statocytes, thereby activating mechanosensitive ion channels at the plasma membrane (Yoder et al., 2001). In turn, channel activation could be responsible, directly or indirectly, for the rapid transients in C ...
Synovial fibroblasts: key players in rheumatoid
... As an obvious advantage of this model, both processes occur in the absence of inflammatory cells or other pro-inflammatory mediators [10, 24]. However, as with every animal model, the extrapolation of these data to human RA patients is difficult, thus limiting direct conclusions. Angiogenesis is anothe ...
... As an obvious advantage of this model, both processes occur in the absence of inflammatory cells or other pro-inflammatory mediators [10, 24]. However, as with every animal model, the extrapolation of these data to human RA patients is difficult, thus limiting direct conclusions. Angiogenesis is anothe ...
On the Morphology of the Drosophila Heart
... Abstract: The circulatory system of Drosophila melanogaster represents an easily amenable genetic model whose analysis at different levels, i.e., from single molecules up to functional anatomy, has provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to na ...
... Abstract: The circulatory system of Drosophila melanogaster represents an easily amenable genetic model whose analysis at different levels, i.e., from single molecules up to functional anatomy, has provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to na ...
Nerve activates contraction
... Connective Tissue Types Dense connective tissue Main matrix element is collagen fibers Cells are fibroblasts ...
... Connective Tissue Types Dense connective tissue Main matrix element is collagen fibers Cells are fibroblasts ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.