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senescent cells
senescent cells

... care expenditures in developed nations. New findings suggest that aging is a modifiable risk factor, and it may be feasible to delay age-related diseases as a group by modulating fundamental aging mechanisms. One such mechanism is cellular senescence, which can cause chronic inflammation through the ...
Bone Marrow Norepinephrine Release in the Spleen and Cells and
Bone Marrow Norepinephrine Release in the Spleen and Cells and

... is influenced (2– 4). Thus, lymphocyte function is closely regulated by a combination of mechanisms associated with both the immune and nervous systems. Norepinephrine (NE)4 is a signaling molecule of the sympathetic nervous system that is released from sympathetic nerve terminals, which are found i ...
Segregation of object and background motion in the
Segregation of object and background motion in the

... reversal of black and white bars, did not significantly alter the cell’s response (Fig. 5a). Nor did such a phase reversal alter the response of polyaxonal amacrine cells (Fig. 5a). Varying the spatial frequency of the grating also had little effect on the firing pattern of the ganglion cells (Fig. ...
Evolution and Development
Evolution and Development

... turbinata, rather than focus on species such as Ciona. Ciona has small larvae that exhibit the conventional mode of development; the larvae exist as free swimming members of the plankton, and during metamorphosis, the larval head attaches to the substrate, the tail is lost and the tissues of the hea ...
Minus End-Directed Kinesin-Like Motor Protein
Minus End-Directed Kinesin-Like Motor Protein

... undergo a profound transformation during mitosis. In Haemanthus endosperm, the metaphase spindle is predominantly barrel-shaped, but usually becomes convergent in anaphase [Smirnova and Bajer, 1994]. The change of spindle shape in anaphase is an important feature of mitosis, preventing formation of ...
The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate
The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate

... which lack the b-subunit of PFT (Cutler et al., 1996; Pei et al., 1998). In addition, two geranylgeranylated proteins, ROP2 and ROP6, have been shown to be involved in negative regulation of ABA signaling (Lemichez et al., 2001; Li et al., 2001; Yang, 2002). Similarly, the existence of geranylgerany ...
TORC2 Signaling is Antagonized by Protein Phosphatase
TORC2 Signaling is Antagonized by Protein Phosphatase

... (Inoki et al. 2005; Wullschleger et al. 2006; Zoncu et al. 2011). The TOR kinase is inhibited by the complex of rapamycin and Fpr1, a peptidyl-prolyl cis-trans isomerase. The TOR kinase is conserved in eukaryotes. Unlike fungal species, which may possess two TOR kinases, higher eukaryotes such as h ...
2281-MC-025 Bax 6A7 for pdf
2281-MC-025 Bax 6A7 for pdf

... Description: The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bax has been shown to redistribute from the cytosol to the mitochondria during apoptosis, and overexpression of Bax can accelerate cell death. Coregulation of Bax dimer formatio ...
video slide - Issaquah Connect
video slide - Issaquah Connect

... Pellet rich in researchers used microscopy to identify the organelles mitochondria in each pellet, establishing a baseline for further experiments. (and chloroplasts if cells In the next series of experiments, researchers used are from a Pellet rich in biochemical methods to determine the metabolic ...
Plant architecture without multicellularity: quandaries over patterning
Plant architecture without multicellularity: quandaries over patterning

Characterization of Dependencies Between Growth and
Characterization of Dependencies Between Growth and

... S/G2 /M’s for mothers compared with daughters for both 6×CLN3 cells and wild-type cells growing in glucose. We also found suggestive (but not significant) differences in S/G2 /M for wild-type cells growing in glycerol/ethanol. One potential explanation for these differences in S/G2 /M duration is th ...
Biology I – 9 weeks review
Biology I – 9 weeks review

... What type of cells in the body does Meiosis take place in? _______________________ How many divisions does a cell go through in Meiosis? ____________________________ What is the process where homologous chromosomes trade parts? _______________________ How many cells are produced after Meiosis? _____ ...
Fate mapping and cell lineage analysis of Hensen`s node in the
Fate mapping and cell lineage analysis of Hensen`s node in the

... dissecting microscope the confocal microscope, and in paraffin sections. A stereo-pair image of a stage 4 Hensen's node obtained by confocal microscopy is shown in Fig. 1. At this stage, the node is a bulbous mass of cells lying at the rostral end of the primitive streak immediately anterior to the ...
Genetic Regulation of Embryonic Pattern Formation
Genetic Regulation of Embryonic Pattern Formation

... of parent-specific imprinting. For example, heterozygosity for mutations in the MEDEA (MEA) gene results in 50% aborted embryos that cannot be rescued by one or even two copies of the paternal wild-type allele, indicating that MEA supports embryo development only when supplied from the female gameto ...
Development
Development

... Development of the primary sexual characteristics depends directly on the endocrine environment during development. An individual can be forced into either a female development or a male development by application of the appropriate hormones, regardless of genetic makeup. In the absence of hormonal ...
Embryonic development of the human hematopoietic system
Embryonic development of the human hematopoietic system

... tissues depends on the colonization of their rudiments by bloodborne hematopoietic progenitor cells. This observation first set the basis for the prevailing idea that the YS be the unique provider of HSC, since no intrinsic hematopoietic potential could be attributed to any other tissue inside the e ...
Powerpoint sel
Powerpoint sel

... Chapter 2 : Cell as a Unit of Life 2.3 Cell Organisation in the Human Body  The types and functions of human cells  Organisation of cells  The system of the human body and their functions 2.4 The Human Being − a Complex Organism  The human being − a complex organism ...
Confocal imaging of glial cells in the intact rat optic nerve
Confocal imaging of glial cells in the intact rat optic nerve

... a number of reasons. 1) Single mature dye-filled glia can be imaged, because LRD does not pass through gap junctions. 2) The entire process field of astrocytes can be visualized in a single two-dimensional image. 3) Cell images can be rotated through 360" in all planes to provide a new perspective o ...
Chapter 2
Chapter 2

... Chapter 2 : Cell as a Unit of Life 2.3 Cell Organisation in the Human Body  The types and functions of human cells  Organisation of cells  The system of the human body and their functions 2.4 The Human Being − a Complex Organism  The human being − a complex organism BM Version ...
Production of final product
Production of final product

... Widely used for assay of various proteins (antigen and antibody) Ex) EPO assay, interferons, therapeutic antibodies, antibody against HIV, Hepatitis B, C virus, Use of enzyme-conjugated secondary antibody for signal amplification : HRP or Alkaline Phosphatase Automated measurement using a 96 well pl ...
01 Signal transduction
01 Signal transduction

... The ability to recognize and respond to pathogen threat is a fundamental requirement of the host to ensure survival. In humans, the innate immune system provides the initial response to this threat via the Tolllike receptor (TLR) family of pattern recognition receptors. TLR activation however is a d ...
a comparative cytological and morphometric analysis of vacuolation
a comparative cytological and morphometric analysis of vacuolation

... Vacuoles play very important physiological roles in plant cells. Pea root nodules, which exhibit distinct zonation (meristematic zone and central tissue zones), may serve as a good experimental model for the investigations of vacuole development and its importance to cell and tissue functioning. Mor ...
Chapter 12 The Cell Cycle Multiple-Choice Questions
Chapter 12 The Cell Cycle Multiple-Choice Questions

... into the culture of dividing human cells at specific times. 41) Which of the following questions might be answered by such a method? A) How many cells are produced by the culture per hour? B) What is the length of the S phase of the cell cycle? C) When is the S chromosome synthesized? D) How many pi ...
The alternative oxidase lowers mitochondrial reactive oxygen
The alternative oxidase lowers mitochondrial reactive oxygen

... work with these transgenic plants showed that changes in the level of AOX within the mitochondria did not have a significant effect on growth rate, except in the presence of antimycin A (10). Under those conditions, cells overexpressing Aox1 grew significantly faster than wild type (WT), whereas cel ...
Microtechnologies for Cell Microenvironment Control and
Microtechnologies for Cell Microenvironment Control and

... Abstract: A great breadth of questions remains in cellular biology. Some questions cannot be answered using traditional analytical techniques and so demand the development of new tools for research. In the near future, the development of highly integrated microfluidic analytical platforms will enabl ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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