Biology YLP 1415 - Revere Public Schools
... further classified into a hierarchical taxonomic systems (kingdom, phylum, class, order, family, genus, species) based on morphological, behavioral, and molecular similarities. Describe the role that geographic isolation can play in speciation. Essential Questions: ...
... further classified into a hierarchical taxonomic systems (kingdom, phylum, class, order, family, genus, species) based on morphological, behavioral, and molecular similarities. Describe the role that geographic isolation can play in speciation. Essential Questions: ...
DBexam1Pt2KEY - Society for Developmental Biology
... (E) [2 pts] accepted ßcatenin (because of sea urchins) but was looking for noggin, chordin, and other “organizer molecules” that, admittedly, are more vertebrate-‐specific (F) [6 pts] Most obvious experimen ...
... (E) [2 pts] accepted ßcatenin (because of sea urchins) but was looking for noggin, chordin, and other “organizer molecules” that, admittedly, are more vertebrate-‐specific (F) [6 pts] Most obvious experimen ...
An Introduction to the Reference Module in Life Sciences
... applications of living systems to derive products predate this definition by millennia. The earliest applications of living organisms to create novel products are in the area of food fermentation. Beer making in China is believed to have occurred at B7000 BCE, followed by yogurt and cheese at B6000 B ...
... applications of living systems to derive products predate this definition by millennia. The earliest applications of living organisms to create novel products are in the area of food fermentation. Beer making in China is believed to have occurred at B7000 BCE, followed by yogurt and cheese at B6000 B ...
Anthraquinone emodin inhibits human cancer
... progression is likely to depend on the acquisition by cancer cells of a more invasive potency (2). At the time being, there is no specific treatment targeting metastases or preventing metastasis development. Moreover, metastatic cells often exhibit resistance to conventional therapies such as chemot ...
... progression is likely to depend on the acquisition by cancer cells of a more invasive potency (2). At the time being, there is no specific treatment targeting metastases or preventing metastasis development. Moreover, metastatic cells often exhibit resistance to conventional therapies such as chemot ...
Geranylgeranylated Proteins are Involved in the Regulation of
... migration, cytoskeletal reorganization, stimulation of cell proliferation, and survival. Activating Ras mutations are frequently detected in myeloma (15 – 17) and contribute to reduced apoptosis (18, 19), increased cell proliferation (18, 19), and an adverse clinical outcome (15, 17). Rac-1, Cdc42, ...
... migration, cytoskeletal reorganization, stimulation of cell proliferation, and survival. Activating Ras mutations are frequently detected in myeloma (15 – 17) and contribute to reduced apoptosis (18, 19), increased cell proliferation (18, 19), and an adverse clinical outcome (15, 17). Rac-1, Cdc42, ...
Lysis by Agents of Microbial Origin
... determined. It might prevent entry into the cell of a key substance necessary for cell-wallsynthesis, or it might inhibit an enzyme taking part in this process. It might also disorganize the cytoplasmic membrane, a structure which is almost certainly involved in the formation of the cell wall. In fa ...
... determined. It might prevent entry into the cell of a key substance necessary for cell-wallsynthesis, or it might inhibit an enzyme taking part in this process. It might also disorganize the cytoplasmic membrane, a structure which is almost certainly involved in the formation of the cell wall. In fa ...
Comparative transcriptional profiling analysis of the two daughter
... model to investigate early embryogenesis because of their development free of maternal tissue [8-10]. However, embryo sac in higher plants is typically surrounded by the sporophytic tissues of ovule and ovary, thus access to the embryo is hampered. To overcome these difficulties, the researchers uti ...
... model to investigate early embryogenesis because of their development free of maternal tissue [8-10]. However, embryo sac in higher plants is typically surrounded by the sporophytic tissues of ovule and ovary, thus access to the embryo is hampered. To overcome these difficulties, the researchers uti ...
Yersinia pestis and plague - Biochemical Society Transactions
... the production of the pro-inflammatory cytokines such as tumour necrosis factor α and interferon γ [13]. ...
... the production of the pro-inflammatory cytokines such as tumour necrosis factor α and interferon γ [13]. ...
What sort of Science is Glycoscience?
... < Sialyl-Tn is a carbohydrate associated with MUC1. It is a unique tumor-associated antigen, present on many adenocarcinomas including breast, ovarian, colorectal, gastric and pancreatic. Consequently, SialylTN is an ideal candidate for boosting the patient’s immune system specifically against a uni ...
... < Sialyl-Tn is a carbohydrate associated with MUC1. It is a unique tumor-associated antigen, present on many adenocarcinomas including breast, ovarian, colorectal, gastric and pancreatic. Consequently, SialylTN is an ideal candidate for boosting the patient’s immune system specifically against a uni ...
A Novel Transfection Method for Mammalian Cells Using Calcium
... have been reported for the transfection of mammalian cells including microinjection (1), particle bombardment (2), the calcium phosphate method (3), the liposome method (4) and the virus-mediated method (5). However, there are limitations in every technique, with some methods requiring complicated p ...
... have been reported for the transfection of mammalian cells including microinjection (1), particle bombardment (2), the calcium phosphate method (3), the liposome method (4) and the virus-mediated method (5). However, there are limitations in every technique, with some methods requiring complicated p ...
Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of
... multimer and cluster formation which is important for many signalling events at the PM. Nevertheless, the exact role of lipid rafts in cellular signalling, trafficking, and structure has yet to be determined. Lipid rafts and raft-associated proteins have also been identified in plants (Borner et al., ...
... multimer and cluster formation which is important for many signalling events at the PM. Nevertheless, the exact role of lipid rafts in cellular signalling, trafficking, and structure has yet to be determined. Lipid rafts and raft-associated proteins have also been identified in plants (Borner et al., ...
Hedgehog proteins: expression and function in the thymus
... and in the formation of peripheral nerve ensheathment(33,34) has been demonstrated. All the described developmental processes are regulated by Hh molecules not only because of their capacity to determine cell fate but also by their ability to modulate cell survival and proliferation. The first evide ...
... and in the formation of peripheral nerve ensheathment(33,34) has been demonstrated. All the described developmental processes are regulated by Hh molecules not only because of their capacity to determine cell fate but also by their ability to modulate cell survival and proliferation. The first evide ...
Voltage-dependent Ionic Conductances of Type I Spiral Ganglion
... bottom. A modified Leibovitz medium (NaCl, 142.2 mM; KCl, 5.37 mM; CaCl,, 1.25 mM; MgCl,, 1.48 mM; HEPES, 5.0 mM; dextrose, 5.0 mM; pH 7.2) was used as the normal perfusate. Modifications to the extracellular medium are noted in figure captions, with NaCl adjusted to maintain osmolarity (300 mOsm). ...
... bottom. A modified Leibovitz medium (NaCl, 142.2 mM; KCl, 5.37 mM; CaCl,, 1.25 mM; MgCl,, 1.48 mM; HEPES, 5.0 mM; dextrose, 5.0 mM; pH 7.2) was used as the normal perfusate. Modifications to the extracellular medium are noted in figure captions, with NaCl adjusted to maintain osmolarity (300 mOsm). ...
Tumor-specific T cells in human Merkel cell carcinomas: a possible
... 2008). A subset of MCC lacked CLA+ T cells, suggesting skin-homing T cells were excluded (Figure 1f). Immunostaining of MCC tumors demonstrated that CLA expression correlated with the pattern of T cell infiltration into tumors. 3/3 MCC with higher levels of T cell CLA expression were infiltrated by ...
... 2008). A subset of MCC lacked CLA+ T cells, suggesting skin-homing T cells were excluded (Figure 1f). Immunostaining of MCC tumors demonstrated that CLA expression correlated with the pattern of T cell infiltration into tumors. 3/3 MCC with higher levels of T cell CLA expression were infiltrated by ...
A theoretical model based upon mast cells and histamine to explain
... dermatitis’. MCs are also present in the heart tissue and their localization is of particular relevance to their function. Data from studies made on interactions of EMFs with the cardiac function have demonstrated that highly interesting changes are present in the heart after exposure to EMFs. One c ...
... dermatitis’. MCs are also present in the heart tissue and their localization is of particular relevance to their function. Data from studies made on interactions of EMFs with the cardiac function have demonstrated that highly interesting changes are present in the heart after exposure to EMFs. One c ...
Formative Cell Divisions: Principal Determinants of Plant
... Plant cells are surrounded by the rigid cell walls and, as a result, they are immobile. Descendant cells are placed next to the mother cell and remain there throughout their lifespan. The orientation of the cell division plane is critical as it determines not only the positions of daughter cells but ...
... Plant cells are surrounded by the rigid cell walls and, as a result, they are immobile. Descendant cells are placed next to the mother cell and remain there throughout their lifespan. The orientation of the cell division plane is critical as it determines not only the positions of daughter cells but ...
extracellular matrix remodeling and integrin
... salivary gland and other organs requires fibronectin. Acting through integrin a5b1, fibronectin locally decreases E-cadherin to stimulate cleft formation. In the lung, cytoskeletal contraction mediated through ROCK signaling is critical for branching morphogenesis. Cytoskeletal contraction leads to ...
... salivary gland and other organs requires fibronectin. Acting through integrin a5b1, fibronectin locally decreases E-cadherin to stimulate cleft formation. In the lung, cytoskeletal contraction mediated through ROCK signaling is critical for branching morphogenesis. Cytoskeletal contraction leads to ...
Mitosis in the Mouse: A Study of Living and
... 2 per cent, osmium tetroxide, usually after being rinsed in warm Tyrode; it is important carefully to drain off the surplus saline in order to ensure instantaneous coagulation of the cells. Tissue cultures are ideal material for cytological purposes, because their extreme thinness allows of almost p ...
... 2 per cent, osmium tetroxide, usually after being rinsed in warm Tyrode; it is important carefully to drain off the surplus saline in order to ensure instantaneous coagulation of the cells. Tissue cultures are ideal material for cytological purposes, because their extreme thinness allows of almost p ...
Origin of muscle satellite cells in the Xenopus embryo
... identify satellite cells. There are also some Pax7-positive cells in the central nervous system at this stage (Chen et al., 2006) but these cannot be confused with muscle satellite cells. The ventrolateral grafts never contributed any cells to the myotomes, but the paraxial and dorsolateral grafts b ...
... identify satellite cells. There are also some Pax7-positive cells in the central nervous system at this stage (Chen et al., 2006) but these cannot be confused with muscle satellite cells. The ventrolateral grafts never contributed any cells to the myotomes, but the paraxial and dorsolateral grafts b ...
THINK ABOUT IT
... The process by which particles move from an area of high concentration to an area of lower concentration is known as diffusion. Diffusion is the driving force behind the movement of many substances across the cell membrane. ...
... The process by which particles move from an area of high concentration to an area of lower concentration is known as diffusion. Diffusion is the driving force behind the movement of many substances across the cell membrane. ...
7. Biology Glossary
... one-celled organisms that form phytoplankton as well as the seaweeds. Although algae photosynthesise and their cells are surrounded by a cell wall, they are not plants. They belong to a group of organisms called the Protoctista. Allele: One of the different forms of a particular gene.In humans, a ge ...
... one-celled organisms that form phytoplankton as well as the seaweeds. Although algae photosynthesise and their cells are surrounded by a cell wall, they are not plants. They belong to a group of organisms called the Protoctista. Allele: One of the different forms of a particular gene.In humans, a ge ...
Roles of ARFRP1 (ADP-ribosylation factor
... play a role in the endosome-to-TGN transport (Yoshino et al., 2003). In addition, Lu et al. have very recently demonstrated that Arl1 and golgin-97 are required for transport from early/recycling endosomes to the TGN (Lu et al., 2004). Mammalian ARFRP1 (ARF-related protein 1), previously designated ...
... play a role in the endosome-to-TGN transport (Yoshino et al., 2003). In addition, Lu et al. have very recently demonstrated that Arl1 and golgin-97 are required for transport from early/recycling endosomes to the TGN (Lu et al., 2004). Mammalian ARFRP1 (ARF-related protein 1), previously designated ...
Microinjection into plant cells of etiolated - Products
... or plant protoplasts is easy to learn, the injection of single cells within plant organs is more challenging. Usually plant cells possess a very rigid cell wall that is per se a problem for microinjection since it requires stable injection capillaries with small diameters. In combination with the ce ...
... or plant protoplasts is easy to learn, the injection of single cells within plant organs is more challenging. Usually plant cells possess a very rigid cell wall that is per se a problem for microinjection since it requires stable injection capillaries with small diameters. In combination with the ce ...
Skeletal muscle contraction
... strands called tropomyosin. When the muscle is at rest, the proteins cover the binding sites for the head of myosin. A third regulatory protein, called troponin, is made up of three subunits—troponin , which binds with Ca++; which binds to the tropomyosin; which then exposes the binding site on acti ...
... strands called tropomyosin. When the muscle is at rest, the proteins cover the binding sites for the head of myosin. A third regulatory protein, called troponin, is made up of three subunits—troponin , which binds with Ca++; which binds to the tropomyosin; which then exposes the binding site on acti ...
Molecular Identification of a SNAP-25
... as a ⬃300-bp fragment from genomic DNA or as a full-length cDNA sequence, were amplified by PCR using the PtSNAP-specific oligonucleotides (Table 1) and cloned into the double T7 promoter plasmid pL4440 (71) over the SpeI and XhoI restriction sites. Plasmids were introduced in the E. coli Ht115 stra ...
... as a ⬃300-bp fragment from genomic DNA or as a full-length cDNA sequence, were amplified by PCR using the PtSNAP-specific oligonucleotides (Table 1) and cloned into the double T7 promoter plasmid pL4440 (71) over the SpeI and XhoI restriction sites. Plasmids were introduced in the E. coli Ht115 stra ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.