Cellartis® iPS Cell to Hepatocyte Differentiation System
... This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc. This product may not be resold or transferred, modified for resale or transfer, or used to manufac ...
... This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc. This product may not be resold or transferred, modified for resale or transfer, or used to manufac ...
Gene transcription is coordinated with, but not dependent on, cell
... transgenes. clk-1(qm30); itIs38; stIs10116 worms were grown at 20°C for over two generations before embryos were imaged at 20°C. Strains containing the temperature-sensitive alleles div-1(or148), emb16(g19) or emb-29(g52) were grown at 15°C before L4 hermaphrodites were incubated overnight at 25°C a ...
... transgenes. clk-1(qm30); itIs38; stIs10116 worms were grown at 20°C for over two generations before embryos were imaged at 20°C. Strains containing the temperature-sensitive alleles div-1(or148), emb16(g19) or emb-29(g52) were grown at 15°C before L4 hermaphrodites were incubated overnight at 25°C a ...
A conditional mutation in Arabidopsis thaliana
... separase activity is regulated and to what extent the protein has acquired other functions. Separase is an essential gene; therefore, studies of separase lossof-function have, for the most part, been limited to tissue culture or early embryos. We know little about how separase acts in the cells of a ...
... separase activity is regulated and to what extent the protein has acquired other functions. Separase is an essential gene; therefore, studies of separase lossof-function have, for the most part, been limited to tissue culture or early embryos. We know little about how separase acts in the cells of a ...
A quantitative atlas of mitotic phosphorylation
... We grew asynchronous HeLa cells in media containing 13C615N2lysine and 13C615N4-arginine (‘‘heavy’’). Cells were mixed with either G1 or M-phase arrested cells grown in conventional media (‘‘light’’) and lysed in 8 M urea (Fig. 1). The mixed whole-cell extracts were directly proteolyzed with trypsin ...
... We grew asynchronous HeLa cells in media containing 13C615N2lysine and 13C615N4-arginine (‘‘heavy’’). Cells were mixed with either G1 or M-phase arrested cells grown in conventional media (‘‘light’’) and lysed in 8 M urea (Fig. 1). The mixed whole-cell extracts were directly proteolyzed with trypsin ...
Bacillus globigii cell size is influenced by
... Next we assessed whether this effect was irreversible or only during the presence of the peptides. For this, treated cells were 1009 diluted in fresh medium without peptides and grown for another 6 h. Then, the cell size was determined again. The white bars in Fig. 2 represent this recovery and it c ...
... Next we assessed whether this effect was irreversible or only during the presence of the peptides. For this, treated cells were 1009 diluted in fresh medium without peptides and grown for another 6 h. Then, the cell size was determined again. The white bars in Fig. 2 represent this recovery and it c ...
Virus-transformed Pre-B Cells Show Ordered Activation but Not
... presence of a hybridizing amplified germline fragment as well as several fragments of predicted lengths using both V and D primers showed that DJ and VDJ rearrangements must have occurred during the growth of these clonal cell lines in culture. Single cell clones of HAFTL A, cultured for several ...
... presence of a hybridizing amplified germline fragment as well as several fragments of predicted lengths using both V and D primers showed that DJ and VDJ rearrangements must have occurred during the growth of these clonal cell lines in culture. Single cell clones of HAFTL A, cultured for several ...
pharmaceutical effects on gene expresson Edited Tambellini
... phenotype. • This technique allows for the quick and easy detection of successful gene integration into plasmid, without the need to individually test each colony. • White colonies = AMPr, LAC Z disrupted • Blue colonies = AMPr , and LAC-z intact ...
... phenotype. • This technique allows for the quick and easy detection of successful gene integration into plasmid, without the need to individually test each colony. • White colonies = AMPr, LAC Z disrupted • Blue colonies = AMPr , and LAC-z intact ...
pdf: Wu et al. 2010
... separase activity is regulated and to what extent the protein has acquired other functions. Separase is an essential gene; therefore, studies of separase lossof-function have, for the most part, been limited to tissue culture or early embryos. We know little about how separase acts in the cells of a ...
... separase activity is regulated and to what extent the protein has acquired other functions. Separase is an essential gene; therefore, studies of separase lossof-function have, for the most part, been limited to tissue culture or early embryos. We know little about how separase acts in the cells of a ...
Developmental plasticity, cell fate specification and morphogenesis
... and compensate for perturbations in the positions and numbers of cells. Removing blastomeres, rearranging them or making chimaeras of more than one embryo can all result in the formation of a blastocyst, indicating a flexibility in cell potential until the 32-cell stage [4–7]. This ability of cells ...
... and compensate for perturbations in the positions and numbers of cells. Removing blastomeres, rearranging them or making chimaeras of more than one embryo can all result in the formation of a blastocyst, indicating a flexibility in cell potential until the 32-cell stage [4–7]. This ability of cells ...
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and
... the C terminus with green fluorescent protein (GFP). After immunoprecipitation using the anti-GFP antibody, we detected SEC10, SEC15b, and EXO70A1 by matrix-assisted laserdesorption ionization (MALDI) time-of-flight mass spectrometry in bands excised from the polyacrylamide gel; these bands were not ...
... the C terminus with green fluorescent protein (GFP). After immunoprecipitation using the anti-GFP antibody, we detected SEC10, SEC15b, and EXO70A1 by matrix-assisted laserdesorption ionization (MALDI) time-of-flight mass spectrometry in bands excised from the polyacrylamide gel; these bands were not ...
Comparison of cryofixation and aldehyde fixation for plant actin
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
PROGRAMMED CELL DEATH IN PLANT DISEASE
... Suicide, in human terms, is intellectually difficult to accept and generally viewed as impulsive or irrational and inconsistent with balanced behavior. Suicide in cellular terms, however, is exactly the converse: it is pervasive, organized, rational, and leads to organismal balance, both in developm ...
... Suicide, in human terms, is intellectually difficult to accept and generally viewed as impulsive or irrational and inconsistent with balanced behavior. Suicide in cellular terms, however, is exactly the converse: it is pervasive, organized, rational, and leads to organismal balance, both in developm ...
differentiation and proliferation of embryonic mast
... cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue--safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based ...
... cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue--safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based ...
7-1 Life Is Cellular
... plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from the reproduction of existing cells. These discoveries led to the cell theory. Slide 7 of 31 Copyright Pearson Prentice Hall ...
... plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from the reproduction of existing cells. These discoveries led to the cell theory. Slide 7 of 31 Copyright Pearson Prentice Hall ...
Some Recent Work on the Structure of the Plant Cell Wall
... contribution that microchemistry is making toward solving the problems of cell wall structure. Microchemical studies of the plant cell wall have revealed it as a complex structure composed of distinct lamellae, the lamellae in turn being composed of one or more different chemical substances. The uni ...
... contribution that microchemistry is making toward solving the problems of cell wall structure. Microchemical studies of the plant cell wall have revealed it as a complex structure composed of distinct lamellae, the lamellae in turn being composed of one or more different chemical substances. The uni ...
Shp1 regulates T cell homeostasis by limiting IL
... accumulation of memory phenotype cells is not driven by a response to a specific endogenous antigen (Fig. 3 D). T cells respond normally to TCR stimulation in the absence of Shp1 To test whether Shp1 plays a role in regulating TCR sensitivity, T cells from Shp1fl/fl and Shp1fl/fl CD4-cre mice were s ...
... accumulation of memory phenotype cells is not driven by a response to a specific endogenous antigen (Fig. 3 D). T cells respond normally to TCR stimulation in the absence of Shp1 To test whether Shp1 plays a role in regulating TCR sensitivity, T cells from Shp1fl/fl and Shp1fl/fl CD4-cre mice were s ...
Robust Genome Editing in Stem Cells Using CRISPR
... effective for generating disease models for both monogenic and complex genetic disorders. For successful genome editing and downstream application of iPSCs, many factors need to be considered, such as choice of growth media, extracellular matrix, genome editing tools, and nucleic acid (NA) delivery ...
... effective for generating disease models for both monogenic and complex genetic disorders. For successful genome editing and downstream application of iPSCs, many factors need to be considered, such as choice of growth media, extracellular matrix, genome editing tools, and nucleic acid (NA) delivery ...
Krebs Cycle Intermediates Protective against Oxidative Stress by
... triphosphate molecules. Many types of fuel molecules can be drawn into and utilized by the cycle, including acetyl coenzyme A, derived from glycolysis or fatty acid oxidation. In eukaryotic cells, most of the enzymes catalyzing the reactions of the Krebs cycle are found in the mitochondrial matrixes ...
... triphosphate molecules. Many types of fuel molecules can be drawn into and utilized by the cycle, including acetyl coenzyme A, derived from glycolysis or fatty acid oxidation. In eukaryotic cells, most of the enzymes catalyzing the reactions of the Krebs cycle are found in the mitochondrial matrixes ...
Viruses and Viral Like Agents
... • About 5, 000 viruses have been identified, but there is believed to be around 400, 000 different viruses. • Believed to be the most abundant biological entity on Earth!! • Viruses are very small- they do not “grow,” just reproduce • They cannot reproduce on their own (obligate, intracellular parti ...
... • About 5, 000 viruses have been identified, but there is believed to be around 400, 000 different viruses. • Believed to be the most abundant biological entity on Earth!! • Viruses are very small- they do not “grow,” just reproduce • They cannot reproduce on their own (obligate, intracellular parti ...
Some Recent Work on the Structure of the Plant Cell Wall
... contribution that microchemistry is making toward solving the problems of cell wall structure. Microchemical studies of the plant cell wall have revealed it as a complex structure composed of distinct lamellae, the lamellae in turn being composed of one or more different chemical substances. The uni ...
... contribution that microchemistry is making toward solving the problems of cell wall structure. Microchemical studies of the plant cell wall have revealed it as a complex structure composed of distinct lamellae, the lamellae in turn being composed of one or more different chemical substances. The uni ...
Show and tell: cell biology of pathogen invasion
... Understanding how plants and pathogens recognize each other and differentiate to establish either a successful or unsuccessful relationship has been a central question in the field of plant–pathogen interactions. Significant advances have been made in the past decade in identifying plant and microbe ...
... Understanding how plants and pathogens recognize each other and differentiate to establish either a successful or unsuccessful relationship has been a central question in the field of plant–pathogen interactions. Significant advances have been made in the past decade in identifying plant and microbe ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.