Changes in Cell Morphology and the Cellular Localization of Protein
... Nurse, 1987). Paul Nurse demonstrated the power of this concept when he applied a crossspecies complementation assay to fission yeast. Using the cdc2ts mutant strain, he systematically transfected an entire human cDNA library into fission yeast. The strain he used had a temperature sensitive mutatio ...
... Nurse, 1987). Paul Nurse demonstrated the power of this concept when he applied a crossspecies complementation assay to fission yeast. Using the cdc2ts mutant strain, he systematically transfected an entire human cDNA library into fission yeast. The strain he used had a temperature sensitive mutatio ...
The Plant Host–Pathogen Interface Cell Wall and Membrane
... factors.2,3 In the absence of the cognate resistance (R) proteins, effectors can function to disable host basal defenses and to release nutrients from host cells, rendering the host susceptible to pathogen proliferation. However, if these effectors are recognized by plant surveillance systems, usual ...
... factors.2,3 In the absence of the cognate resistance (R) proteins, effectors can function to disable host basal defenses and to release nutrients from host cells, rendering the host susceptible to pathogen proliferation. However, if these effectors are recognized by plant surveillance systems, usual ...
T-Cell Activation by Recombinant Receptors
... the two-signal paradigm, resting T cells can be either preactivated by incubation with an anti-CD3 mab in addition to exogenous IL-2 or by anti-CD28-specific mabs (6) that mimick signaling mediated by receptor molecules. CD28 costimulation, in addition to signaling through the TCR/CD3 complex, is on ...
... the two-signal paradigm, resting T cells can be either preactivated by incubation with an anti-CD3 mab in addition to exogenous IL-2 or by anti-CD28-specific mabs (6) that mimick signaling mediated by receptor molecules. CD28 costimulation, in addition to signaling through the TCR/CD3 complex, is on ...
GFP-tagging of cell components reveals the dynamics of subcellular
... 776 Daigo Takemoto et al. myces (Heath and Heath, 1971), barley–Erysiphe (Kunoh et al., 1985), parsley–Phytophthora (Gross et al., 1993), flax– Melampsora (Kobayashi et al., 1994), onion–Magnaporthe (Xu et al., 1998), and onion–Botrytis (McLusky et al., 1999) interactions. This indicates that cytop ...
... 776 Daigo Takemoto et al. myces (Heath and Heath, 1971), barley–Erysiphe (Kunoh et al., 1985), parsley–Phytophthora (Gross et al., 1993), flax– Melampsora (Kobayashi et al., 1994), onion–Magnaporthe (Xu et al., 1998), and onion–Botrytis (McLusky et al., 1999) interactions. This indicates that cytop ...
The Extracellular Matrix
... • Isolated due to its ability to cluster pre-existing acetylcholine receptors in skeletal muscle fibers • BM form binds to laminin ...
... • Isolated due to its ability to cluster pre-existing acetylcholine receptors in skeletal muscle fibers • BM form binds to laminin ...
Wnt Signaling Translocates Lys48-Linked
... degradation (Nathan et al., 2013). Ubiquitin molecules are recycled back by proteasome-associated deubiquitinating enzymes (Lee et al., 2011), and proteins targeted for endolysosomal degradation are thought to be deubiquitinated before incorporation into MVBs (Piper and Katzmann, 2007). Thus, ubiqui ...
... degradation (Nathan et al., 2013). Ubiquitin molecules are recycled back by proteasome-associated deubiquitinating enzymes (Lee et al., 2011), and proteins targeted for endolysosomal degradation are thought to be deubiquitinated before incorporation into MVBs (Piper and Katzmann, 2007). Thus, ubiqui ...
Shape engineering vs organic modification of cellular internalization
... Full list of author information is available at the end of the article ...
... Full list of author information is available at the end of the article ...
CpG-ODN 2006 and human parvovirus B19 genome consensus
... erythroid (GPA⫹), neutrophilic (CD15⫹), and megakaryocytic (CD61⫹) progenitors occurred. We found that 2006-PO inhibited the growth of erythroid progenitors in a dose-dependent manner but did not affect the growth of neutrophilic and megakaryocytic progenitors (Figure 1A). To investigate the specifi ...
... erythroid (GPA⫹), neutrophilic (CD15⫹), and megakaryocytic (CD61⫹) progenitors occurred. We found that 2006-PO inhibited the growth of erythroid progenitors in a dose-dependent manner but did not affect the growth of neutrophilic and megakaryocytic progenitors (Figure 1A). To investigate the specifi ...
Campbell Biology, 10e (Reece) Chapter 7 Membrane Structure and
... 22) Which of the following allows water to move much faster across cell membranes? A) the sodium-potassium pump B) ATP C) peripheral proteins D) aquaporins Answer: D Bloom's Taxonomy: Knowledge/Comprehension Section: 7.2 23) You are working on a team that is designing a new drug. For this drug to wo ...
... 22) Which of the following allows water to move much faster across cell membranes? A) the sodium-potassium pump B) ATP C) peripheral proteins D) aquaporins Answer: D Bloom's Taxonomy: Knowledge/Comprehension Section: 7.2 23) You are working on a team that is designing a new drug. For this drug to wo ...
Effect of n-butanol and cold pretreatment on the cytoskeleton and
... Production of double haploid (DH) plants is a valuable tool in conventional breeding. This technique reduces the time needed for the development of new, improved varieties. Developing microspores of higher plants possess the ability to switch their default gametophytic developmental program to the s ...
... Production of double haploid (DH) plants is a valuable tool in conventional breeding. This technique reduces the time needed for the development of new, improved varieties. Developing microspores of higher plants possess the ability to switch their default gametophytic developmental program to the s ...
The neural tube origin of ventral root sheath cells in
... labelled crestless neural tube grafts, he again observed that sheath cells emigrate from the neural tube with the motor axons, but could not determine the full extent of this emigration because of the transitory nature of the marker. Using the stable quail marker (Le Douarin, 1973), our results conf ...
... labelled crestless neural tube grafts, he again observed that sheath cells emigrate from the neural tube with the motor axons, but could not determine the full extent of this emigration because of the transitory nature of the marker. Using the stable quail marker (Le Douarin, 1973), our results conf ...
The effect of temperature shifts on protein synthesis
... times with 5 ml cold 10% TCA. These fractions were treated for counting of radioactivity as described above. Analysis ofproteins by two-dimensional gel electrophoresis. Cells grown to the mid-exponential phase at 0 "C were immediately labelled with [ I4C]leucine (12.9 GBq mmol-l, 74 KBq ml-l) for 60 ...
... times with 5 ml cold 10% TCA. These fractions were treated for counting of radioactivity as described above. Analysis ofproteins by two-dimensional gel electrophoresis. Cells grown to the mid-exponential phase at 0 "C were immediately labelled with [ I4C]leucine (12.9 GBq mmol-l, 74 KBq ml-l) for 60 ...
Structural determinants for rCNT2 sorting to the plasma membrane
... 1 % antibiotic mixture (100 units/ml penicillin G, 0.1 mg/ml streptomycin and 0.25 μg/ml fungizone), 2 mM glutamine, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate. All reagents were purchased from Invitrogen. The MDCK (Madin–Darby canine kidney) II cell line was cultured in DMEM (Dulbecc ...
... 1 % antibiotic mixture (100 units/ml penicillin G, 0.1 mg/ml streptomycin and 0.25 μg/ml fungizone), 2 mM glutamine, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate. All reagents were purchased from Invitrogen. The MDCK (Madin–Darby canine kidney) II cell line was cultured in DMEM (Dulbecc ...
Cyclin synthesis and degradation and the embryonic cell cycle
... required for the exit from meiosis. To address these issues, we turned to in vitro cell cycle extracts of frog eggs that were prepared by an adaptation of the method pioneered by Lohka and Masui (1983). These cell cycle extracts are capable of several cell cycles that proceed at rates similar to tho ...
... required for the exit from meiosis. To address these issues, we turned to in vitro cell cycle extracts of frog eggs that were prepared by an adaptation of the method pioneered by Lohka and Masui (1983). These cell cycle extracts are capable of several cell cycles that proceed at rates similar to tho ...
The Mechanisms and Mechanics of Archenteron Elongation during
... walls, and which have a relatively short distance to traverse during secondary invagination. The first result of the mechanical simulations is that when such shapes are modeled as elastic structures solely under the influence of filopodial pulling, the correct morphology cannot be obtained (Figs. Za ...
... walls, and which have a relatively short distance to traverse during secondary invagination. The first result of the mechanical simulations is that when such shapes are modeled as elastic structures solely under the influence of filopodial pulling, the correct morphology cannot be obtained (Figs. Za ...
Inducibility and Expression of Microvascular
... the epidermis. One part was immediately embedded in OCT (Miles, Naperville, IL), snap frozen, and stored at -70 ' C. The other half was placed in skin organ cultures as described previously [15]. In brief, skin specimens were placed in C24-well plates (Falcon, Bedford, MA) with the epidermis facing ...
... the epidermis. One part was immediately embedded in OCT (Miles, Naperville, IL), snap frozen, and stored at -70 ' C. The other half was placed in skin organ cultures as described previously [15]. In brief, skin specimens were placed in C24-well plates (Falcon, Bedford, MA) with the epidermis facing ...
Moss Systems Biology en Route
... all stages of its life cycle can be grown axenically and photoautotrophically in a simple medium consisting of inorganic salts and water. Moreover, moss cells offer an exceptionally high regeneration capacity under hormone-free conditions (Hohe and Reski, 2005). In contrast to cultivation of seed pl ...
... all stages of its life cycle can be grown axenically and photoautotrophically in a simple medium consisting of inorganic salts and water. Moreover, moss cells offer an exceptionally high regeneration capacity under hormone-free conditions (Hohe and Reski, 2005). In contrast to cultivation of seed pl ...
Gram stain reagents - Bakersfield College
... for staining is important to ensure good results. Remember, you cannot see the material you are working with so you must develop good technique based upon principles. Always start with clean slides using lens paper to clean them. Slides can be made from direct clinical material (a wound, sputum, kne ...
... for staining is important to ensure good results. Remember, you cannot see the material you are working with so you must develop good technique based upon principles. Always start with clean slides using lens paper to clean them. Slides can be made from direct clinical material (a wound, sputum, kne ...
jaf op den kamp*, w. van - Utrecht University Repository
... reported to be identical to lysyl phosphatidyl glycerol n. Chromatographic comparison with a chemically synthesized substance 1~ confirmed this conclusion. The two other major phospholipids were demonstrated to be identical with phosphatidyl glycerol and phosphatidyl ethanolamine. A minor spot exhib ...
... reported to be identical to lysyl phosphatidyl glycerol n. Chromatographic comparison with a chemically synthesized substance 1~ confirmed this conclusion. The two other major phospholipids were demonstrated to be identical with phosphatidyl glycerol and phosphatidyl ethanolamine. A minor spot exhib ...
Product Manual - GenTarget Inc
... Note: at the time of transduction, cells should be 50%-75% confluent. For example, seed HeLa cells at 0.5 x 105/ml x 0.5ml in a well of a 24well plate. Day 1: Thaw the pre-made lentiviral stock at room temperature and add the appropriate amount of virus stock to obtain the desired MOI. Return ce ...
... Note: at the time of transduction, cells should be 50%-75% confluent. For example, seed HeLa cells at 0.5 x 105/ml x 0.5ml in a well of a 24well plate. Day 1: Thaw the pre-made lentiviral stock at room temperature and add the appropriate amount of virus stock to obtain the desired MOI. Return ce ...
Cdc2-mediated Schwann cell migration during peripheral nerve
... Fig. 2. Cdc2 expression in cultured Schwann cells. (A) Western blot analysis of Cdc2 in cultured Schwann cells. Schwann cells were prepared from sciatic nerves given crush injury for 0-14 days or from the sciatic nerve of the rat of postnatal day 3 (pnd3). Cells were incubated for 2 days in 10% seru ...
... Fig. 2. Cdc2 expression in cultured Schwann cells. (A) Western blot analysis of Cdc2 in cultured Schwann cells. Schwann cells were prepared from sciatic nerves given crush injury for 0-14 days or from the sciatic nerve of the rat of postnatal day 3 (pnd3). Cells were incubated for 2 days in 10% seru ...
Cell polarity and tissue patterning in plants - Development
... An advantage of the "canalization hypothesis' is that it suggests various experimental approaches to cellular patterning (Sachs, 1981) and the one to be followed here is to search for additional cellular expressions of the same canalized controls of polarization. The 'canalization hypothesis' consid ...
... An advantage of the "canalization hypothesis' is that it suggests various experimental approaches to cellular patterning (Sachs, 1981) and the one to be followed here is to search for additional cellular expressions of the same canalized controls of polarization. The 'canalization hypothesis' consid ...
Archaea - The Ancient Oddities
... Cell wall structure (for example, some archaeal cell walls contain pseudomurein) ...
... Cell wall structure (for example, some archaeal cell walls contain pseudomurein) ...
BD Pharmingen™ Transcription Factor Buffer Set
... Where can I “stop” in the protocol? Investigators should determine their own “stop” condition for their application. However, here are a few tips: 1. Storing fix/perm samples: We have had success using the 1X fix/perm buffer and then storing overnight at 2–8°C with no manipulations, and then continu ...
... Where can I “stop” in the protocol? Investigators should determine their own “stop” condition for their application. However, here are a few tips: 1. Storing fix/perm samples: We have had success using the 1X fix/perm buffer and then storing overnight at 2–8°C with no manipulations, and then continu ...
Oxidation of Hg(0) - Department of Marine and Coastal Sciences
... frequently physically confined and gas exchange with surroundings is limited. Thus, Hg(0) can potentially interact with metabolically diverse microorganisms including nitrate-reducing, iron-reducing and fermentative bacteria. Because many anaerobes lack catalase enzymes, the mechanism of Hg(0) oxidat ...
... frequently physically confined and gas exchange with surroundings is limited. Thus, Hg(0) can potentially interact with metabolically diverse microorganisms including nitrate-reducing, iron-reducing and fermentative bacteria. Because many anaerobes lack catalase enzymes, the mechanism of Hg(0) oxidat ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.