Mechanism of efficient transfection of the nasal airway
... Effect of tonicity of the DNA solution on transfection of the nasal tissue We evaluated gene transfer in the mouse nose, a commonly used model of the airway epithelium because of the easy access,14 with plasmid DNA (pDNA) in the deionized water, sodium chloride and phosphatebuffered saline (PBS). Lu ...
... Effect of tonicity of the DNA solution on transfection of the nasal tissue We evaluated gene transfer in the mouse nose, a commonly used model of the airway epithelium because of the easy access,14 with plasmid DNA (pDNA) in the deionized water, sodium chloride and phosphatebuffered saline (PBS). Lu ...
Stomatal development - The Company of Biologists
... Bergmann and Sack, 2007; Pillitteri and Torii, 2012). This stomatal lineage consists of five major cell types (Fig. 1): meristemoid mother cells (MMCs; see Glossary, Box 1), meristemoids (see Glossary, Box 1), stomatal lineage ground cells (SLGCs; see Glossary, Box 1), guard mother cells (GMCs; see ...
... Bergmann and Sack, 2007; Pillitteri and Torii, 2012). This stomatal lineage consists of five major cell types (Fig. 1): meristemoid mother cells (MMCs; see Glossary, Box 1), meristemoids (see Glossary, Box 1), stomatal lineage ground cells (SLGCs; see Glossary, Box 1), guard mother cells (GMCs; see ...
Effects of dietary components on Tight junctions (TJ) Lauric acid
... pathogenesis is induced by gliadin, a glycoprotein present in wheat. When IEC6 and Caco2 cells are exposed to gliadin in vitro, interaction between occludin and ZO-1 is compromised and the cytoskeleton is rearranged, leading to increased monolayer permeability (73). The mechanism for this has been l ...
... pathogenesis is induced by gliadin, a glycoprotein present in wheat. When IEC6 and Caco2 cells are exposed to gliadin in vitro, interaction between occludin and ZO-1 is compromised and the cytoskeleton is rearranged, leading to increased monolayer permeability (73). The mechanism for this has been l ...
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... Bergmann and Sack, 2007; Pillitteri and Torii, 2012). This stomatal lineage consists of five major cell types (Fig. 1): meristemoid mother cells (MMCs; see Glossary, Box 1), meristemoids (see Glossary, Box 1), stomatal lineage ground cells (SLGCs; see Glossary, Box 1), guard mother cells (GMCs; see ...
... Bergmann and Sack, 2007; Pillitteri and Torii, 2012). This stomatal lineage consists of five major cell types (Fig. 1): meristemoid mother cells (MMCs; see Glossary, Box 1), meristemoids (see Glossary, Box 1), stomatal lineage ground cells (SLGCs; see Glossary, Box 1), guard mother cells (GMCs; see ...
Genome-Wide RNAi Screen for Host Factors Required for
... This phenotype was similar to the phenotype observed during infection by DH-L1137 (fig. S1A) and is consistent with a defect in vacuole escape. However, bacteria in dsRNA treated wells appeared to be capable of increased replication within the localized clusters (Fig. 1E). (iii) Increased GFP fluor ...
... This phenotype was similar to the phenotype observed during infection by DH-L1137 (fig. S1A) and is consistent with a defect in vacuole escape. However, bacteria in dsRNA treated wells appeared to be capable of increased replication within the localized clusters (Fig. 1E). (iii) Increased GFP fluor ...
Thiorhodospira sibirica gen. nov., sp. nov., a new alkaliphilic purple
... plant mass in the near-shore area of the soda lake Malyi Kasytui (pH 9 5 , 0 2 % salinity) located in the steppe of the Chita region of south-east Siberia. Single cells were vibrioid- or spiral-shaped ( 3 4 pm wide and 7-20 pm long) and motile by means of a polar tuft of flagella. Internal photosynt ...
... plant mass in the near-shore area of the soda lake Malyi Kasytui (pH 9 5 , 0 2 % salinity) located in the steppe of the Chita region of south-east Siberia. Single cells were vibrioid- or spiral-shaped ( 3 4 pm wide and 7-20 pm long) and motile by means of a polar tuft of flagella. Internal photosynt ...
Gibberellins accumulate in the elongating endodermal cells of
... University of California at San Diego, La Jolla, CA 92093; and cPlant Biology Laboratory, and dHoward Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037 ...
... University of California at San Diego, La Jolla, CA 92093; and cPlant Biology Laboratory, and dHoward Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037 ...
Hemocyte marker proteins and regulation of the proPO system in a
... also that transglutaminase is essential for the maintenance of the Hpt cells in their stem cell form. Further we recently also isolated and characterised the membrane receptor for the cytokine, astakine which is invoved in hematopoiesis (Lin et al., 2007). Prophenoloxidase activating system (proPO-s ...
... also that transglutaminase is essential for the maintenance of the Hpt cells in their stem cell form. Further we recently also isolated and characterised the membrane receptor for the cytokine, astakine which is invoved in hematopoiesis (Lin et al., 2007). Prophenoloxidase activating system (proPO-s ...
Quantitative reverse transcription polymerase chain reaction
... et al., 2001). However, proteins are more stable and have a longer half-life inside the cell than bacterial RNAs. Thus, RNAs are more suitable as cell viability markers for the study of the VBNC state than proteins (Sheridan et al., 1998; Lleo et al., 2000; Fischer-Le Saux et al., 2002). This fact w ...
... et al., 2001). However, proteins are more stable and have a longer half-life inside the cell than bacterial RNAs. Thus, RNAs are more suitable as cell viability markers for the study of the VBNC state than proteins (Sheridan et al., 1998; Lleo et al., 2000; Fischer-Le Saux et al., 2002). This fact w ...
Chapter 3: Observing Microorganisms Through a Microscope
... u The smaller the distance between objects at which they can be distinguished as separate, the greater the resolving power. u Light must pass between two objects in order for them to be seen as separate. u Depends on light wavelength. If wavelength is too long to pass between objects, they will appe ...
... u The smaller the distance between objects at which they can be distinguished as separate, the greater the resolving power. u Light must pass between two objects in order for them to be seen as separate. u Depends on light wavelength. If wavelength is too long to pass between objects, they will appe ...
Gibberellin-Induced α
... to a particular form (gibberellin A1) before they can have any biological effect. Gibberellins can substitute for the dormancy breaking treatments (cold or light) in certain seeds like lettuce tobacco and wild oats. Gibberellins promotes in germination and radicle growth in malting barley seeds. Ext ...
... to a particular form (gibberellin A1) before they can have any biological effect. Gibberellins can substitute for the dormancy breaking treatments (cold or light) in certain seeds like lettuce tobacco and wild oats. Gibberellins promotes in germination and radicle growth in malting barley seeds. Ext ...
Heterarchy of transcription factors driving basal and luminal
... gamma (PPARg) is widely known as an essential and sufficient driver of adipogenesis,1,2 but it also plays roles in M1 to M2 polarisation of macrophages3 and differentiation of human urothelial cells of the bladder and associated urinary tract.4–6 When grown in vitro in the absence of serum or other ...
... gamma (PPARg) is widely known as an essential and sufficient driver of adipogenesis,1,2 but it also plays roles in M1 to M2 polarisation of macrophages3 and differentiation of human urothelial cells of the bladder and associated urinary tract.4–6 When grown in vitro in the absence of serum or other ...
Melanization and Hemocyte Homeostasis in the Freshwater
... form of antibodies, which are proteins that are produced by cells of lymphoid lineage within the adaptive immune system (Jiravanichpaisal et al. 2006). The innate system is an important first line of defense that discriminates self from non-self molecules and recognizes conserved molecules produced ...
... form of antibodies, which are proteins that are produced by cells of lymphoid lineage within the adaptive immune system (Jiravanichpaisal et al. 2006). The innate system is an important first line of defense that discriminates self from non-self molecules and recognizes conserved molecules produced ...
Class I MHC Molecule Protein in Association with an Allogeneic
... proteins (3). 2C CTL specifically responds to Ld in association with the naturally processed peptide LSPFPFDL (p2Ca), isolated from spleen and other tissues (4), or the longer natural peptide VAITRIEQLSPFPFDL (p2Cb), isolated from the same source and containing the entire sequence of p2Ca (5). Both ...
... proteins (3). 2C CTL specifically responds to Ld in association with the naturally processed peptide LSPFPFDL (p2Ca), isolated from spleen and other tissues (4), or the longer natural peptide VAITRIEQLSPFPFDL (p2Cb), isolated from the same source and containing the entire sequence of p2Ca (5). Both ...
Patterning and morphogenesis of the follicle cell epithelium during
... synthesized in follicle cells and fat bodies. In a stage-10A egg chamber, the oocyte occupies about half of the egg chamber. A sheet of somatic follicle cells is uniformly distributed over the germ line cells from stage 1 to 8 (Fig. 1A). However, starting at stage 9, a series of follicle cell migrat ...
... synthesized in follicle cells and fat bodies. In a stage-10A egg chamber, the oocyte occupies about half of the egg chamber. A sheet of somatic follicle cells is uniformly distributed over the germ line cells from stage 1 to 8 (Fig. 1A). However, starting at stage 9, a series of follicle cell migrat ...
BioWire_Progress_Report_Week_One_Rev_1
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
... [Components will be ordered Monday] Replicate Weiss sender cell, adding GFP as a reporter Manually add aTc to the system in varying concentrations Control: Add water instead of aTc Expected Result: GFP is expressed, but not in control plates. ...
Get cached
... azole and polyene drugs (Canuto & Rodero, 2002) there is a requirement for new drugs with alternative modes of action or with the ability to increase the efficacy of existing prescription drugs. Many metalbased drugs display modes of action distinct to those of the prescription anti-fungals (Coyle e ...
... azole and polyene drugs (Canuto & Rodero, 2002) there is a requirement for new drugs with alternative modes of action or with the ability to increase the efficacy of existing prescription drugs. Many metalbased drugs display modes of action distinct to those of the prescription anti-fungals (Coyle e ...
Developmental roles of Rhomboid proteases
... ARTICLE IN PRESS B.-Z. Shilo / Seminars in Cell & Developmental Biology xxx (2016) xxx–xxx ...
... ARTICLE IN PRESS B.-Z. Shilo / Seminars in Cell & Developmental Biology xxx (2016) xxx–xxx ...
Chapter 4
... Three fates of substances made in the membrane system: 1. Secreted proteins made in RER, travel through Golgi, then are exported through plasma membrane – The following figure illustrates this process for antibodies – Antibodies are proteins produced by white blood cells to inactivate foreign diseas ...
... Three fates of substances made in the membrane system: 1. Secreted proteins made in RER, travel through Golgi, then are exported through plasma membrane – The following figure illustrates this process for antibodies – Antibodies are proteins produced by white blood cells to inactivate foreign diseas ...
Nematode lecture
... Functions of Parasitism Proteins Nematodes need to penetrate and migrate through the roots ! ...
... Functions of Parasitism Proteins Nematodes need to penetrate and migrate through the roots ! ...
Mudskipper gill MR cell ion-transport proteins
... (0.8 mg ml−1). Fixed/frozen dogfish (Squalus acanthias) rectal gland tissue was used as a positive control. The T4 monoclonal antibody has greater species cross-reactivity than the J3 monoclonal antibody, the immunoreactivity of which is limited to shark tissues (Lytle et al., 1995). Na+/H+ exchange ...
... (0.8 mg ml−1). Fixed/frozen dogfish (Squalus acanthias) rectal gland tissue was used as a positive control. The T4 monoclonal antibody has greater species cross-reactivity than the J3 monoclonal antibody, the immunoreactivity of which is limited to shark tissues (Lytle et al., 1995). Na+/H+ exchange ...
Staphylococcus aureus-induced G2/M phase transition delay
... To stain phosphorylated H3 histone (p-Ser10 Histone H3), synchronized HeLa cells were exposed to the MW2 strain (MOI 20:1) and immunofluorescence was performed as described [38]. After 4% PFA fixation, cells were permeabilized with 0.3% Triton X-100/PBS solution and then incubated in 10% goat serum ...
... To stain phosphorylated H3 histone (p-Ser10 Histone H3), synchronized HeLa cells were exposed to the MW2 strain (MOI 20:1) and immunofluorescence was performed as described [38]. After 4% PFA fixation, cells were permeabilized with 0.3% Triton X-100/PBS solution and then incubated in 10% goat serum ...
Expression of a Nondegradable Cyclin B1 Affects
... cyclin construct was poorly efficient, but 10 independent transformants could finally be recovered (called CycB1Mut1-to-10). One of these plants, CycB1Mut8, was not fertile and thus was not studied further. The other nine CycB1Mut plants were selfpollinated, and segregation analyses indicated a vari ...
... cyclin construct was poorly efficient, but 10 independent transformants could finally be recovered (called CycB1Mut1-to-10). One of these plants, CycB1Mut8, was not fertile and thus was not studied further. The other nine CycB1Mut plants were selfpollinated, and segregation analyses indicated a vari ...
Fe Sparing and Fe Recycling Contribute to
... Plants require water, CO2, light, and a few inorganic nutrients for growth. Changes in the availability of each/any of these essential ingredients of plant life generate system-wide changes in gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, wher ...
... Plants require water, CO2, light, and a few inorganic nutrients for growth. Changes in the availability of each/any of these essential ingredients of plant life generate system-wide changes in gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, wher ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.