Fe Sparing and Fe Recycling Contribute to
... Plants require water, CO2, light, and a few inorganic nutrients for growth. Changes in the availability of each/any of these essential ingredients of plant life generate system-wide changes in gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, wher ...
... Plants require water, CO2, light, and a few inorganic nutrients for growth. Changes in the availability of each/any of these essential ingredients of plant life generate system-wide changes in gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, wher ...
Staphylococcus aureus-induced G2/M phase transition delay
... To stain phosphorylated H3 histone (p-Ser10 Histone H3), synchronized HeLa cells were exposed to the MW2 strain (MOI 20:1) and immunofluorescence was performed as described [38]. After 4% PFA fixation, cells were permeabilized with 0.3% Triton X-100/PBS solution and then incubated in 10% goat serum ...
... To stain phosphorylated H3 histone (p-Ser10 Histone H3), synchronized HeLa cells were exposed to the MW2 strain (MOI 20:1) and immunofluorescence was performed as described [38]. After 4% PFA fixation, cells were permeabilized with 0.3% Triton X-100/PBS solution and then incubated in 10% goat serum ...
Evaluation of the lactic acid consumption in yeast cultures by
... currently recognized are associated with foods and beverages [3]. In addition, about one-half of the total listed yeast species contains strains that are able to use organic acids as carbon and energy source [4] and thus they can potentially be a serious threat in the food industry. ...
... currently recognized are associated with foods and beverages [3]. In addition, about one-half of the total listed yeast species contains strains that are able to use organic acids as carbon and energy source [4] and thus they can potentially be a serious threat in the food industry. ...
Glucocorticoid-Induced Plasma Membrane Depolarization during
... intracellular environment caused by the loss of potassium is required for subsequent activation of caspase 3-like enzymes and DNA degradation (15–17). Recent studies from our laboratory have shown that antiFas antibody treatment of human Jurkat T cells led to sustained depolarization of the plasma m ...
... intracellular environment caused by the loss of potassium is required for subsequent activation of caspase 3-like enzymes and DNA degradation (15–17). Recent studies from our laboratory have shown that antiFas antibody treatment of human Jurkat T cells led to sustained depolarization of the plasma m ...
Inhibition of Replication of Herpes Simplex Virus
... interferon (IFN). Different types of mouse IFN (c~, fl, ~,) exhibited similar antiviral activities. However, treatment of cells with IFN-~ in combination with IEN-~ or IFN/3 resulted in a synergistic inhibition of virus growth. As shown by assaying HSV D N A polymerase, IFN inhibited expression of t ...
... interferon (IFN). Different types of mouse IFN (c~, fl, ~,) exhibited similar antiviral activities. However, treatment of cells with IFN-~ in combination with IEN-~ or IFN/3 resulted in a synergistic inhibition of virus growth. As shown by assaying HSV D N A polymerase, IFN inhibited expression of t ...
IL-10 transcription is negatively regulated by BAF180, a component
... Aging Intramural Research Program, National Institutes of Health, Baltimore, USA Full list of author information is available at the end of the article ...
... Aging Intramural Research Program, National Institutes of Health, Baltimore, USA Full list of author information is available at the end of the article ...
Interplay between cell growth and cell cycle in
... blocks required for plant growth, but the space that can be occupied by these building blocks is constrained by the cell walls. Cell enlargement requires that the walls yield to the cell’s turgor pressure and cell-wall relaxation appears to be the major control point in this process (Wolf et al., 20 ...
... blocks required for plant growth, but the space that can be occupied by these building blocks is constrained by the cell walls. Cell enlargement requires that the walls yield to the cell’s turgor pressure and cell-wall relaxation appears to be the major control point in this process (Wolf et al., 20 ...
TRANSPORT OF SODIUM, POTASSIUM, AND CALCIUM ACROSS
... whether or not the observed concentration gradients of Na § and K + across rabbit leukocyte membranes are maintained by means of a "Na, K pump". The results of two representative experiments on the effect of ouabain on the time course of K § influx and Na § efflux are depicted in Figs. 5 and 6, resp ...
... whether or not the observed concentration gradients of Na § and K + across rabbit leukocyte membranes are maintained by means of a "Na, K pump". The results of two representative experiments on the effect of ouabain on the time course of K § influx and Na § efflux are depicted in Figs. 5 and 6, resp ...
Expression of Recombinant Myelin
... cell to develop polarized cell surfaces, and thereby allow a critical number of MAG molecules to interact with receptors on an axon destined to be myelinated (26). Recently, we have found that Schwann cells arrested at the 1:1 stage of Schwann cell/axon association by depletion of galactocerebroside ...
... cell to develop polarized cell surfaces, and thereby allow a critical number of MAG molecules to interact with receptors on an axon destined to be myelinated (26). Recently, we have found that Schwann cells arrested at the 1:1 stage of Schwann cell/axon association by depletion of galactocerebroside ...
Role for a bidentate ribonuclease in the initiation step of RNA
... gene silencing (PTGS) is accomplished by a multicomponent nuclease that targets mRNAs for degradation6,8,11. The speci®city of this complex may derive from the incorporation of a small guide sequence that is homologous to the mRNA substrate6. These ,22-nucleotide RNAs, originally identi®ed in plants ...
... gene silencing (PTGS) is accomplished by a multicomponent nuclease that targets mRNAs for degradation6,8,11. The speci®city of this complex may derive from the incorporation of a small guide sequence that is homologous to the mRNA substrate6. These ,22-nucleotide RNAs, originally identi®ed in plants ...
Sample Chapter
... symbolized µm. A human egg cell is about 140 µm in diameter and is just barely visible to an unaided eye. This is large when compared to a red blood cell, which is about 7.5 µm in diameter, or the most common types of white blood cells, which vary from 10 to 12 µm in diameter. On the other hand, smo ...
... symbolized µm. A human egg cell is about 140 µm in diameter and is just barely visible to an unaided eye. This is large when compared to a red blood cell, which is about 7.5 µm in diameter, or the most common types of white blood cells, which vary from 10 to 12 µm in diameter. On the other hand, smo ...
Calmodulin-binding protein disrupts mitosis
... The yeast spindle pole body (SPB) is a microtubule organising centre, functionally equivalent to the centrosome in more complex eukaryotes, that is responsible for the ordered generation of the microtubular spindle in M phase (for a recent review see Hagan, 1998). A recent report highlighted the imp ...
... The yeast spindle pole body (SPB) is a microtubule organising centre, functionally equivalent to the centrosome in more complex eukaryotes, that is responsible for the ordered generation of the microtubular spindle in M phase (for a recent review see Hagan, 1998). A recent report highlighted the imp ...
Sample
... 5. What is the primary factor governing penetration of alcohols (and similar compounds) through biological membranes? Their lipid solubility. 6. Why are different concentrations of alcohols used in this experiment? To shorten the penetration times so that the experiment can be completed during the l ...
... 5. What is the primary factor governing penetration of alcohols (and similar compounds) through biological membranes? Their lipid solubility. 6. Why are different concentrations of alcohols used in this experiment? To shorten the penetration times so that the experiment can be completed during the l ...
Synthesis and Breakdown of Poly -p-Hydroxybutyric
... The occurrence of poly-P-hydroxybutyric acid in the genus Bacillus was reported by Lemoigne, Delaporte & Croson (1944), who were able to correlate the presence of prominent refractile granules in the cells with the isolation of a wax-like, chloroform-soluble, ether-insoluble polymer from the large-c ...
... The occurrence of poly-P-hydroxybutyric acid in the genus Bacillus was reported by Lemoigne, Delaporte & Croson (1944), who were able to correlate the presence of prominent refractile granules in the cells with the isolation of a wax-like, chloroform-soluble, ether-insoluble polymer from the large-c ...
Input of DNA microarrays to identify novel mechanisms - HAL
... majority of the patients surviving more that 10 years after initiation of total therapy (43 ). Identification of myeloma growth factors Based on GEP data, we have identified new families of proteins which play a major role in the biology of the MM, and which are therefore promising therapeutic targe ...
... majority of the patients surviving more that 10 years after initiation of total therapy (43 ). Identification of myeloma growth factors Based on GEP data, we have identified new families of proteins which play a major role in the biology of the MM, and which are therefore promising therapeutic targe ...
Yantar, a conserved arginine-rich protein is involved in Drosophila
... tumor phenotype, and we named their corresponding genes yantar (ytr), polka dots (pkdt), and splotchy (splo). Hemocytes from these mutants were visualized after immunostaining using two hemocyte-specific monoclonal antibodies, MAb H2 and L1. MAb H2 recognizes the hemese antigen present in all Drosop ...
... tumor phenotype, and we named their corresponding genes yantar (ytr), polka dots (pkdt), and splotchy (splo). Hemocytes from these mutants were visualized after immunostaining using two hemocyte-specific monoclonal antibodies, MAb H2 and L1. MAb H2 recognizes the hemese antigen present in all Drosop ...
Synthesis and Breakdown of Poly -p-Hydroxybutyric
... The occurrence of poly-P-hydroxybutyric acid in the genus Bacillus was reported by Lemoigne, Delaporte & Croson (1944), who were able to correlate the presence of prominent refractile granules in the cells with the isolation of a wax-like, chloroform-soluble, ether-insoluble polymer from the large-c ...
... The occurrence of poly-P-hydroxybutyric acid in the genus Bacillus was reported by Lemoigne, Delaporte & Croson (1944), who were able to correlate the presence of prominent refractile granules in the cells with the isolation of a wax-like, chloroform-soluble, ether-insoluble polymer from the large-c ...
Lesson 4 Reading Material: Angiogenesis and Metastasis
... cell can travel through the blood and eventually exit out into another organ. For example, a breast cancer cell that has recruited new blood vessels, can enter the blood vessels and travel to a distant site such as the lung, and exit there and begin growing another tumor in the lung. This process is ...
... cell can travel through the blood and eventually exit out into another organ. For example, a breast cancer cell that has recruited new blood vessels, can enter the blood vessels and travel to a distant site such as the lung, and exit there and begin growing another tumor in the lung. This process is ...
A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose
... Actin were used as internal controls. All data are means of three replicates from independent experiments, with error bars indicating SE. ...
... Actin were used as internal controls. All data are means of three replicates from independent experiments, with error bars indicating SE. ...
Botanical Gazette
... concentrated near the epidermis that stained NINand PAS-positive; microsporophylls were not examined. All idioblasts and epidermal cells stained for tannins. Most idioblasts showed transfer cell wall-like structures and evidence of breakdown. Few druses were found in the sporophyll tissues. No starc ...
... concentrated near the epidermis that stained NINand PAS-positive; microsporophylls were not examined. All idioblasts and epidermal cells stained for tannins. Most idioblasts showed transfer cell wall-like structures and evidence of breakdown. Few druses were found in the sporophyll tissues. No starc ...
Associative learning increases adult neurogenesis during a critical
... likely to survive in response to learning than cells in animals that are exposed to training but do not learn. Because neurogenesis is an ongoing and overlapping process, it is possible that learning differentially affects new cells as a function of their maturity. To address this issue, we examined ...
... likely to survive in response to learning than cells in animals that are exposed to training but do not learn. Because neurogenesis is an ongoing and overlapping process, it is possible that learning differentially affects new cells as a function of their maturity. To address this issue, we examined ...
The plant formin AtFH4 interacts with both actin and microtubules
... material Fig. S3), suggesting that the presence of AtFH4D4 promotes microtubule bundling. Together, these data show that the GOE domain exhibits microtubule-binding activity in vivo and in vitro, and that the neighbouring FH1 region also influences AtFH4microtubule interactions. In animals, three fo ...
... material Fig. S3), suggesting that the presence of AtFH4D4 promotes microtubule bundling. Together, these data show that the GOE domain exhibits microtubule-binding activity in vivo and in vitro, and that the neighbouring FH1 region also influences AtFH4microtubule interactions. In animals, three fo ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.