![Characterization of the pH of Folate Receptor](http://s1.studyres.com/store/data/014872380_1-4c3a59361c2ac36baa38430c7bc560db-300x300.png)
Characterization of the pH of Folate Receptor
... Cell Culture. KB cells, a human nasopharyngeal cancer cell line, was grown continuously as a monolayer using folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) containing 10% heat-inactivated fetal calf serum (Hyclone, Logan, UT) at 37°C in a 5% CO2/95% air-humidified atmosphere in the presence ...
... Cell Culture. KB cells, a human nasopharyngeal cancer cell line, was grown continuously as a monolayer using folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) containing 10% heat-inactivated fetal calf serum (Hyclone, Logan, UT) at 37°C in a 5% CO2/95% air-humidified atmosphere in the presence ...
Detection of secreted peptides by using hypothesis
... left spectra in Fig. 1 show partial MALDI mass spectra of the extracts obtained by placing a small quantity of each of the three cell types in the MALDI matrix solution. The mass spectra encompass the region m兾z 1,550–1,750, where we expect to observe the Mf peptides. In no case do we observe the Mf ...
... left spectra in Fig. 1 show partial MALDI mass spectra of the extracts obtained by placing a small quantity of each of the three cell types in the MALDI matrix solution. The mass spectra encompass the region m兾z 1,550–1,750, where we expect to observe the Mf peptides. In no case do we observe the Mf ...
Cells are Either Prokaryotic or Eukaryotic
... between plant cells, the plasmodesmata, allow for the movement of materials among cells. Thanks to the plasmodesmata channels, the cytoplasm of one cell is continuous with the cytoplasm of the next; the plant as a whole can be thought of as having a single complement of continuous ...
... between plant cells, the plasmodesmata, allow for the movement of materials among cells. Thanks to the plasmodesmata channels, the cytoplasm of one cell is continuous with the cytoplasm of the next; the plant as a whole can be thought of as having a single complement of continuous ...
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... for methanogenic rods, and probably also for the methanogenic rods of Mastigella. As a result of methane production by these endosymbionts, the intracellular hydrogen levels may remain very low and, as a consequence, the eukaryote can easily reoxidize its reduced electron acceptors and profit from a ...
... for methanogenic rods, and probably also for the methanogenic rods of Mastigella. As a result of methane production by these endosymbionts, the intracellular hydrogen levels may remain very low and, as a consequence, the eukaryote can easily reoxidize its reduced electron acceptors and profit from a ...
RNA Tumour Virus Phosphoproteins
... distribution patterns of its variously charged molecular species by urea-polyacrylamide gradient gel electrophoresis. The distribution patterns of the pI 2 of two different field isolates of WM viruses, 292 and I5O4, and the mouse-tropic and amphotropic clonal sub-populations of 15o4 field isolate w ...
... distribution patterns of its variously charged molecular species by urea-polyacrylamide gradient gel electrophoresis. The distribution patterns of the pI 2 of two different field isolates of WM viruses, 292 and I5O4, and the mouse-tropic and amphotropic clonal sub-populations of 15o4 field isolate w ...
Chapter 1: The Microbial World and You
... Light must pass between two objects in order for them to be seen as separate. Depends on light wavelength. If wavelength is too long to pass between objects, they will appear as one. White light has a relatively long wavelength (550 nm), and cannot resolve structures less than 220 nm (0.2 um) a ...
... Light must pass between two objects in order for them to be seen as separate. Depends on light wavelength. If wavelength is too long to pass between objects, they will appear as one. White light has a relatively long wavelength (550 nm), and cannot resolve structures less than 220 nm (0.2 um) a ...
Science as a way of learning
... o Growth and Development: Possess DNA (inherited information) to function o Energy utilization: can transform food into energy o Response to environment: can respond to stumli o Reproduction: can reproduce through information in DNA & make more cells o They evolved from other things Life is Also O ...
... o Growth and Development: Possess DNA (inherited information) to function o Energy utilization: can transform food into energy o Response to environment: can respond to stumli o Reproduction: can reproduce through information in DNA & make more cells o They evolved from other things Life is Also O ...
PPT - Altogen Biosystems
... Products > Transfection Reagent for BALB/3T3 Cells (Embryo Fibroblast Cells, CCL-163) Altogen Biosystems offers the BALB/3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The BALB/3T3 Transfection Reagent is a nanoparticle-based liposome formulation reagent ...
... Products > Transfection Reagent for BALB/3T3 Cells (Embryo Fibroblast Cells, CCL-163) Altogen Biosystems offers the BALB/3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The BALB/3T3 Transfection Reagent is a nanoparticle-based liposome formulation reagent ...
Ch. 5 ppt
... A. Cells are arranged in tissues that provide specific functions for the body. B. Cells of different tissues are structured differently, which leads to their differences in function. C. The tissues of the human body include four major types: epithelial, connective, muscle, and nervous. ...
... A. Cells are arranged in tissues that provide specific functions for the body. B. Cells of different tissues are structured differently, which leads to their differences in function. C. The tissues of the human body include four major types: epithelial, connective, muscle, and nervous. ...
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... that give rise to each of these cells and by their characteristic positions within the embryo. Following injection of any of these identified cells with a cell lineage tracer during stages 6 or 7, a distinct and invariant segmentally repeated pattern of labeled progeny cells is observed later in the ...
... that give rise to each of these cells and by their characteristic positions within the embryo. Following injection of any of these identified cells with a cell lineage tracer during stages 6 or 7, a distinct and invariant segmentally repeated pattern of labeled progeny cells is observed later in the ...
Cytotoxic T Cells Sufficient to Induce Primary and Memory Soluble
... priming (11). The factors that empower the DC are unknown, but it is known that CD40 triggering can up-regulate costimulatory molecules and inflammatory cytokines such as IL-12 (22). The essential costimulatory requirements for generation of T cell memory are not defined. Although CD28-B7 interactio ...
... priming (11). The factors that empower the DC are unknown, but it is known that CD40 triggering can up-regulate costimulatory molecules and inflammatory cytokines such as IL-12 (22). The essential costimulatory requirements for generation of T cell memory are not defined. Although CD28-B7 interactio ...
Ch. 5 ppt - USD305.com
... A. Cells are arranged in tissues that provide specific functions for the body. B. Cells of different tissues are structured differently, which leads to their differences in function. C. The tissues of the human body include four major types: epithelial, connective, muscle, and nervous. ...
... A. Cells are arranged in tissues that provide specific functions for the body. B. Cells of different tissues are structured differently, which leads to their differences in function. C. The tissues of the human body include four major types: epithelial, connective, muscle, and nervous. ...
The src
... What Causes Cancer? - Random mutations (mistakes at the assembly line) - Inherited mutations (pre-disposition) - Viral infections - Environmental factors (chemical; physical) Viral infection as a cause for cancer is not common in humans, but it does exist (human papillomavirus can cause carcinoma o ...
... What Causes Cancer? - Random mutations (mistakes at the assembly line) - Inherited mutations (pre-disposition) - Viral infections - Environmental factors (chemical; physical) Viral infection as a cause for cancer is not common in humans, but it does exist (human papillomavirus can cause carcinoma o ...
Drug development in oncology assisted by - HAL
... linked to the RAFT-RGD part was mainly localized at cell junctions but was also found in small vesicles, as previously described for RAFT-RGD alone (Sancey et al., 2009). Most of the KLA, pseudo-colored in “red” was in the close vicinity of the vector RAFT-RGD or detached into the medium near the ce ...
... linked to the RAFT-RGD part was mainly localized at cell junctions but was also found in small vesicles, as previously described for RAFT-RGD alone (Sancey et al., 2009). Most of the KLA, pseudo-colored in “red” was in the close vicinity of the vector RAFT-RGD or detached into the medium near the ce ...
Gram Stain - American Proficiency Institute
... Certain conditions are known to damage the cell wall, causing gram-positive bacteria to falsely appear gram-negative or gram-variable. These include antibiotic treatment, cultures more than 48 hours old, inflammatory responses in the host, and autolytic enzymes (e.g., S. pneumoniae). To minimize amb ...
... Certain conditions are known to damage the cell wall, causing gram-positive bacteria to falsely appear gram-negative or gram-variable. These include antibiotic treatment, cultures more than 48 hours old, inflammatory responses in the host, and autolytic enzymes (e.g., S. pneumoniae). To minimize amb ...
Corneal epithelial cell biocompatibility to silicone
... materials, they have the potential to sorb components of the packaging solution during storage, which could then be subsequently released upon insertion of the CL on the eye. The purpose of this study was to investigate the effect of various packaging solutions on HCECs, using an in vitro model. Met ...
... materials, they have the potential to sorb components of the packaging solution during storage, which could then be subsequently released upon insertion of the CL on the eye. The purpose of this study was to investigate the effect of various packaging solutions on HCECs, using an in vitro model. Met ...
Tropism of human cytomegalovirus for endothelial cells is
... Cells. Human foreskin fibroblasts (HFF) were cultured in MEM containing 2n4 mmol\l glutamine, 100 µg\ml gentamicin and 5 % foetal calf serum (MEM5). Fibroblasts were used for experiments between passages 10 and 25. Human umbilical vein endothelial cells (HUVEC) were cultured in RPMI 1640 containing ...
... Cells. Human foreskin fibroblasts (HFF) were cultured in MEM containing 2n4 mmol\l glutamine, 100 µg\ml gentamicin and 5 % foetal calf serum (MEM5). Fibroblasts were used for experiments between passages 10 and 25. Human umbilical vein endothelial cells (HUVEC) were cultured in RPMI 1640 containing ...
Hematopoiesis
... decrease. The kidneys will sense that, and produce EPO, which stimulates stem cells to divide faster, and hastens the release of immature RBCs into the bloodstream, so we will see more reticulocytes in a blood smear. ...
... decrease. The kidneys will sense that, and produce EPO, which stimulates stem cells to divide faster, and hastens the release of immature RBCs into the bloodstream, so we will see more reticulocytes in a blood smear. ...
Cooperative organization of bacterial colonies: from genotype to
... fluid. In the event a walker’s step would lead to its movement outside the boundary, the step is not performed, and a counter on the appropriate segment of the envelope is increased by one. When a segment counter reaches a threshold [N.sub.c], the envelope segment propagates, adding one lattice area ...
... fluid. In the event a walker’s step would lead to its movement outside the boundary, the step is not performed, and a counter on the appropriate segment of the envelope is increased by one. When a segment counter reaches a threshold [N.sub.c], the envelope segment propagates, adding one lattice area ...
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... if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional cells in which there was a clear heterogeneous distribution of surface stain but it was not organized into a coherent pole. % Polarized includes all ...
... if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional cells in which there was a clear heterogeneous distribution of surface stain but it was not organized into a coherent pole. % Polarized includes all ...
Activation of β-Catenin in Dendritic Cells Regulates
... Whether β-catenin signaling in DCs is critical for intestinal homeostasis. We compared the frequencies of Treg cells and TH17/TH1 cells in the intestine of β-catDC−/− and β-catfl/fl mice because intestinal DCs and macrophages play an important role in the induction of these subsets. β-catDC−/− mice ...
... Whether β-catenin signaling in DCs is critical for intestinal homeostasis. We compared the frequencies of Treg cells and TH17/TH1 cells in the intestine of β-catDC−/− and β-catfl/fl mice because intestinal DCs and macrophages play an important role in the induction of these subsets. β-catDC−/− mice ...
Mutations affecting development of zebrafish digestive organs
... formation. The pancreas and liver originate as endodermal buds from the foregut. During maturation of the intestine, the lining cells develop from a simple cuboidal to a polarized columnar epithelium composed of several distinctive cell types. Outside the basement membrane there is growth of connect ...
... formation. The pancreas and liver originate as endodermal buds from the foregut. During maturation of the intestine, the lining cells develop from a simple cuboidal to a polarized columnar epithelium composed of several distinctive cell types. Outside the basement membrane there is growth of connect ...
Ectopic expression of fibroblast growth factor
... resulted in IL-6 independence, decreased apoptosis, and an enhanced proliferative response to IL-6. In the presence of ligand, wild-type FGFR3-expressing cells also exhibited enhanced proliferation and survival in comparison to controls. B9 clones expressing either wild-type FGFR3 at high levels or ...
... resulted in IL-6 independence, decreased apoptosis, and an enhanced proliferative response to IL-6. In the presence of ligand, wild-type FGFR3-expressing cells also exhibited enhanced proliferation and survival in comparison to controls. B9 clones expressing either wild-type FGFR3 at high levels or ...
transcription factor foxc2 demarcates the jugular lymphangiogenic
... lymphangiogenesis, when lymphatic collectors become invested with contractile smooth muscle cells and develop valves, which control the direction of lymph flow. Early functions of FoxC2 seem to reside in the induction of lymphatic sprouts from embryonic veins, and loss of FoxC2 can be rescued by the ...
... lymphangiogenesis, when lymphatic collectors become invested with contractile smooth muscle cells and develop valves, which control the direction of lymph flow. Early functions of FoxC2 seem to reside in the induction of lymphatic sprouts from embryonic veins, and loss of FoxC2 can be rescued by the ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.