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Now! - ambition classes
... studying human evolution are called anthropologists. Studies have revealed that human evolution started in Africa and earliest human type was Austaloplthecus Africanus. African ape man fossil was discovered by Prof. Raymond Dart fossil of skull of 5-6 years old baby from old pliocene rock of Tuang r ...
... studying human evolution are called anthropologists. Studies have revealed that human evolution started in Africa and earliest human type was Austaloplthecus Africanus. African ape man fossil was discovered by Prof. Raymond Dart fossil of skull of 5-6 years old baby from old pliocene rock of Tuang r ...
Ciliary neurotrophic factor maintains the pluripotentiality of
... LIF is thus far the only factor known that can maintain ES cells, which are derived from the inner cell mass of a preimplantation mouse blastocyst, in an undifferentiated state capable of contributing to all cell lineages upon reintroduction into the embryo (Smith et al., 1988; Williams et al., 1988 ...
... LIF is thus far the only factor known that can maintain ES cells, which are derived from the inner cell mass of a preimplantation mouse blastocyst, in an undifferentiated state capable of contributing to all cell lineages upon reintroduction into the embryo (Smith et al., 1988; Williams et al., 1988 ...
Isolation and purification of cell wall polysaccharide of Bacillus
... A polysaccharide fraction was isolated from sodium-dodecyl-sulfate (SDS) treated cell walls of Bacillus anthrac& (A Sterne) by hydrofluoric acid (HF) hydrolysis and ethanolic precipitation. The polysaccharide fraction was subsequently purified by several washings with absolute ethanol. Purity of the ...
... A polysaccharide fraction was isolated from sodium-dodecyl-sulfate (SDS) treated cell walls of Bacillus anthrac& (A Sterne) by hydrofluoric acid (HF) hydrolysis and ethanolic precipitation. The polysaccharide fraction was subsequently purified by several washings with absolute ethanol. Purity of the ...
Molecular General and Genetics
... swpn1, were isolated and characterized from suspension-cultured cells of sweet potato in order to understand the physiological function of POD isozymes. Sequence analysis showed that swpa1 encoded an anionic POD and swpn1 encoded a neutral POD. The swpa1 and swpn1 genes were both highly expressed in ...
... swpn1, were isolated and characterized from suspension-cultured cells of sweet potato in order to understand the physiological function of POD isozymes. Sequence analysis showed that swpa1 encoded an anionic POD and swpn1 encoded a neutral POD. The swpa1 and swpn1 genes were both highly expressed in ...
Tolerance to mutations in the foot-and
... integrins, and 213hs”, a derivative highly adapted to cell culture whose ability to infect cells using the glycosaminoglycan heparan sulfate (HS) as receptor, acquired by multiple passage on BHK21 cells, has been abolished. The capsid sequence context determined infectivity in cultured cells and dir ...
... integrins, and 213hs”, a derivative highly adapted to cell culture whose ability to infect cells using the glycosaminoglycan heparan sulfate (HS) as receptor, acquired by multiple passage on BHK21 cells, has been abolished. The capsid sequence context determined infectivity in cultured cells and dir ...
Directional Terms, Planes, and Cavities Flash Cards Anatomy and
... List the two classifications of glandular epithelia. A18 Endocrine Exocrine Q19 What is the difference between endocrine and exocrine glands? A19 Endocrine Ductless Secretes hormones directly into extracellular space (or blood) Exocrine May or may not have ducts Secretes product directly on body su ...
... List the two classifications of glandular epithelia. A18 Endocrine Exocrine Q19 What is the difference between endocrine and exocrine glands? A19 Endocrine Ductless Secretes hormones directly into extracellular space (or blood) Exocrine May or may not have ducts Secretes product directly on body su ...
The Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and
... Cell Culture—The cell lines used for these studies have been described previously (11, 19, 20). 143B.TK⫺ is a human osteosarcomaderived cell line (ATCC CRL 8303); the 94I, 43B, 2SA, 2SC, 4H1, and pT1 cell lines are mitochondrial transformants of an mtDNA-less (0) derivative of 143B.TK⫺, i.e. 0206 ...
... Cell Culture—The cell lines used for these studies have been described previously (11, 19, 20). 143B.TK⫺ is a human osteosarcomaderived cell line (ATCC CRL 8303); the 94I, 43B, 2SA, 2SC, 4H1, and pT1 cell lines are mitochondrial transformants of an mtDNA-less (0) derivative of 143B.TK⫺, i.e. 0206 ...
FIP2 and Rip11 specify Rab11a-mediated cellular
... second interesting member of the class I FIPs is Rip11 which also co-localises with Rab11a in endosomal membranes [15]. To investigate the functional role of Rab11a and its effector proteins Rip11 and FIP2 in GLUT4 and FAT/ CD36 translocation we established a small interfering RNA (siRNA) knockdown a ...
... second interesting member of the class I FIPs is Rip11 which also co-localises with Rab11a in endosomal membranes [15]. To investigate the functional role of Rab11a and its effector proteins Rip11 and FIP2 in GLUT4 and FAT/ CD36 translocation we established a small interfering RNA (siRNA) knockdown a ...
Lysosomal Heterogeneity in Exocrine Acinar Cells
... the American and Japanese Histochemical Societies, held in Vancouver, British Columbia, Canada, July 20-24, 1982. ...
... the American and Japanese Histochemical Societies, held in Vancouver, British Columbia, Canada, July 20-24, 1982. ...
LETTERS
... (Fig. 2u). In similar experiments in which only control red and green reporters were electroporated, cells grafted anteriorly in the streak were always found to lie more anterior in the somitic series (data not shown, Supplementary Table 1). These experiments indicate that overexpressing more poster ...
... (Fig. 2u). In similar experiments in which only control red and green reporters were electroporated, cells grafted anteriorly in the streak were always found to lie more anterior in the somitic series (data not shown, Supplementary Table 1). These experiments indicate that overexpressing more poster ...
Asymmetric cell division in C. elegans - Development
... Here, we show that, in addition to regulating cell migrations, EGL-20 also influences the polarity of certain asymmetric cell divisions in the lateral epidermis of the animal. The six epidermal V cells (V1-V6), also known as seam cells, divide in a polarized pattern along the A/P axis during the pos ...
... Here, we show that, in addition to regulating cell migrations, EGL-20 also influences the polarity of certain asymmetric cell divisions in the lateral epidermis of the animal. The six epidermal V cells (V1-V6), also known as seam cells, divide in a polarized pattern along the A/P axis during the pos ...
Nuclear Factor of Activated T Cells - JEM
... plasmid (NFAT3x-Luc) containing three copies of the distal NFAT site of the murine IL-2 promoter (21), was provided by Dr. D. McKean (Mayo Clinic, Rochester, MN). The GAL4luciferase reporter plasmid (GAL4-Luc) (22), the cDNA plasmids pCMVb-HA-p300, pCMVb-E1A (23), the E1A mutant defective in binding ...
... plasmid (NFAT3x-Luc) containing three copies of the distal NFAT site of the murine IL-2 promoter (21), was provided by Dr. D. McKean (Mayo Clinic, Rochester, MN). The GAL4luciferase reporter plasmid (GAL4-Luc) (22), the cDNA plasmids pCMVb-HA-p300, pCMVb-E1A (23), the E1A mutant defective in binding ...
Lineage-restricted neural precursors survive, migrate, and
... the culturing period, the cells showed the characteristic morphology of small, phase-bright, lineage-restricted precursors (Fig. 1E). Before transplantation, the phenotypic composition of the grafted cells was again verified. We found that at the time of grafting, all cells in the NRP/GRP cultures e ...
... the culturing period, the cells showed the characteristic morphology of small, phase-bright, lineage-restricted precursors (Fig. 1E). Before transplantation, the phenotypic composition of the grafted cells was again verified. We found that at the time of grafting, all cells in the NRP/GRP cultures e ...
Standard PDF - Wiley Online Library
... CD45RA, or CD69 is shown for LN cells (A). Dot plots are representative of 34 independent experiments using one freshly collected LN biopsy per experiment. This strategy is similar for PBMCs. Frequencies of PBMCs (B) and LN (C) total CD4+ T cells within the CD3+ T cells and subsets within the CD4+ T ...
... CD45RA, or CD69 is shown for LN cells (A). Dot plots are representative of 34 independent experiments using one freshly collected LN biopsy per experiment. This strategy is similar for PBMCs. Frequencies of PBMCs (B) and LN (C) total CD4+ T cells within the CD3+ T cells and subsets within the CD4+ T ...
the Adult Drosophila Fat Body
... tetroxide, and prepared for electron microscopy, following methods listed in Butterworth et ai. (1988). Thin sections were stained with uranyl acetate and lead citrate. In some cases morphometric analysis was performed at the light- and electron-microscopic level according to the methods in Butterwo ...
... tetroxide, and prepared for electron microscopy, following methods listed in Butterworth et ai. (1988). Thin sections were stained with uranyl acetate and lead citrate. In some cases morphometric analysis was performed at the light- and electron-microscopic level according to the methods in Butterwo ...
Cellular Maturation Defects in Bruton`s Tyrosine
... In Btk-deficient mice, ⫹ and ⫹ immature B cells show differences in cellular maturation During the progression of large cycling into small resting c H chain-positive pre-B cells, Btk-deficient mice manifest defective down-regulation of BP-1, CD43, and SLC and impaired up-regulation of CD2, CD25, ...
... In Btk-deficient mice, ⫹ and ⫹ immature B cells show differences in cellular maturation During the progression of large cycling into small resting c H chain-positive pre-B cells, Btk-deficient mice manifest defective down-regulation of BP-1, CD43, and SLC and impaired up-regulation of CD2, CD25, ...
Electrophysiological properties of mouse bone marrow c
... Recent reports have shown that ES cells can be induced to differentiate into cardiac myocytes by embryonic activation pathways like those elicited by BMP-4 and TGF-h molecules [3] or visceral endoderm tissue [2]. Additionally, HSCs are affected in differentiation and proliferation/self-renewal pathw ...
... Recent reports have shown that ES cells can be induced to differentiate into cardiac myocytes by embryonic activation pathways like those elicited by BMP-4 and TGF-h molecules [3] or visceral endoderm tissue [2]. Additionally, HSCs are affected in differentiation and proliferation/self-renewal pathw ...
Choose your fate: artery, vein or lymphatic vessel?
... which the expression of genes is gained, or repressed, to induce arterial or lymphatic cell fate. It is also possible that AECs might differentiate directly from angioblasts without proceeding via a venous phase. In mammals, LECs originate from the cardinal veins following the induction of expressio ...
... which the expression of genes is gained, or repressed, to induce arterial or lymphatic cell fate. It is also possible that AECs might differentiate directly from angioblasts without proceeding via a venous phase. In mammals, LECs originate from the cardinal veins following the induction of expressio ...
Detoxification of Arsenic by Phytochelatins in Plants
... weight, respectively), corresponding to an approximate ratio of three thiol groups to 1 As. A similar toxicity of As was observed in Arabidopsis seedlings (Fig. 3) by using inhibition of root growth and a well-established marker for metal toxicity (Howden and Cobbett, 1992). Root growth was analyzed ...
... weight, respectively), corresponding to an approximate ratio of three thiol groups to 1 As. A similar toxicity of As was observed in Arabidopsis seedlings (Fig. 3) by using inhibition of root growth and a well-established marker for metal toxicity (Howden and Cobbett, 1992). Root growth was analyzed ...
PPT - Altogen Biosystems
... Transfection Reagent (Prostate Cancer Cells) Altogen Biosystems offers the PC-3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The PC-3 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transf ...
... Transfection Reagent (Prostate Cancer Cells) Altogen Biosystems offers the PC-3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The PC-3 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transf ...
Plasma Membrane Permeability of Root
... 0.5 h pretreatment with Al. K+ release from and Al permeation into the protoplasts isolated from the root-tip of Alsensitive plant species were more significant than those for Al-tolerant plant species (rice and maize) on 10 or 30 min treatment with Al. The permeability of the plasma membrane for pr ...
... 0.5 h pretreatment with Al. K+ release from and Al permeation into the protoplasts isolated from the root-tip of Alsensitive plant species were more significant than those for Al-tolerant plant species (rice and maize) on 10 or 30 min treatment with Al. The permeability of the plasma membrane for pr ...
Transcriptional insights into the CD8+ T cell response to infection
... expression of key gene signatures can be used to predict the memory-precursor potential of CD8 + effector cells. Long-lived memory CD8+ cells ultimately expressed a small subset of genes shared by natural killer T and gd T cells. Although distinct inflammatory milieu and T cell precursor frequencies ...
... expression of key gene signatures can be used to predict the memory-precursor potential of CD8 + effector cells. Long-lived memory CD8+ cells ultimately expressed a small subset of genes shared by natural killer T and gd T cells. Although distinct inflammatory milieu and T cell precursor frequencies ...
Tissues
... Functions of Connective Tissue o Establishing a structural framework for the body o Transporting fluids and dissolved materials o Protecting delicate organs o Supporting, surrounding, and interconnecting other types of tissue o Storing energy reserves, especially in the form of triglycerides o Defen ...
... Functions of Connective Tissue o Establishing a structural framework for the body o Transporting fluids and dissolved materials o Protecting delicate organs o Supporting, surrounding, and interconnecting other types of tissue o Storing energy reserves, especially in the form of triglycerides o Defen ...
Detection of secreted peptides by using hypothesis
... left spectra in Fig. 1 show partial MALDI mass spectra of the extracts obtained by placing a small quantity of each of the three cell types in the MALDI matrix solution. The mass spectra encompass the region m兾z 1,550–1,750, where we expect to observe the Mf peptides. In no case do we observe the Mf ...
... left spectra in Fig. 1 show partial MALDI mass spectra of the extracts obtained by placing a small quantity of each of the three cell types in the MALDI matrix solution. The mass spectra encompass the region m兾z 1,550–1,750, where we expect to observe the Mf peptides. In no case do we observe the Mf ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.