![Anticancer Properties of Cardiac Glycosides](http://s1.studyres.com/store/data/006796137_1-df991d9ad3b0c7023f971f7b78a49ca2-300x300.png)
Anticancer Properties of Cardiac Glycosides
... that an increase in the intracellular Ca2+ concentration following treatment with digoxin, digitoxin, or ouabain is associated with the antiproliferative effects of these compounds in androgen-dependent and androgen-independent prostate cancer cell lines [40]. Because Ca2+ serves as a mediator in se ...
... that an increase in the intracellular Ca2+ concentration following treatment with digoxin, digitoxin, or ouabain is associated with the antiproliferative effects of these compounds in androgen-dependent and androgen-independent prostate cancer cell lines [40]. Because Ca2+ serves as a mediator in se ...
Suppression of Pyk2 Kinase and Cellular Activities by FIP200
... dissociation of endogenous FIP200–Pyk2 complex, which provided further support for inhibition of Pyk2 by FIP200 in intact cells. Together, these results suggest that FIP200 functions as an inhibitor of Pyk2 via binding to its kinase domain. Key words: phosphorylation • FAK • tyrosine kinase • inhibi ...
... dissociation of endogenous FIP200–Pyk2 complex, which provided further support for inhibition of Pyk2 by FIP200 in intact cells. Together, these results suggest that FIP200 functions as an inhibitor of Pyk2 via binding to its kinase domain. Key words: phosphorylation • FAK • tyrosine kinase • inhibi ...
Print - Circulation Research
... be stressed that the myocardium is a heterogeneous structure containing vascular endothelium, vascular smooth muscle, nerves, and fibroblasts in addition to cardiac muscle. It is not possible to determine, at the present time, whether any of these other cell types contribute significantly to the ext ...
... be stressed that the myocardium is a heterogeneous structure containing vascular endothelium, vascular smooth muscle, nerves, and fibroblasts in addition to cardiac muscle. It is not possible to determine, at the present time, whether any of these other cell types contribute significantly to the ext ...
The Gram Reaction and Cell Composition: Nucleic
... We included Bacillus brevis in the Gram-negativegroup of organisms because, in spite of its staining character, it possesses all the normal physiological characteristics of the Gram-positive aerobic spore-forming rods. The fact that B . brevis lacks XSP adds considerable weight to the correlation be ...
... We included Bacillus brevis in the Gram-negativegroup of organisms because, in spite of its staining character, it possesses all the normal physiological characteristics of the Gram-positive aerobic spore-forming rods. The fact that B . brevis lacks XSP adds considerable weight to the correlation be ...
N-Linked glycans on dengue viruses grown in mammalian and
... This study compared the ability of mosquito and mammalian cell-derived dengue virus (DENV) to infect human dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN)-expressing cells and characterized the structure of envelope (E) protein N-linked glycans on DENV derived from the two cell types. ...
... This study compared the ability of mosquito and mammalian cell-derived dengue virus (DENV) to infect human dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN)-expressing cells and characterized the structure of envelope (E) protein N-linked glycans on DENV derived from the two cell types. ...
145 CHAPTER SUMMARY
... 2. The decrease in mass and viability seen in most tissues during old age often reflects circulatory deficits or poor nutrition. ...
... 2. The decrease in mass and viability seen in most tissues during old age often reflects circulatory deficits or poor nutrition. ...
Development of Conjunctival Goblet Cells and Their
... weight glycoconjugates including mucins, which are secreted mainly by conjunctival goblet cells. This layer plays an important role in protecting the ocular surface from exogenous agents (bacterial or chemical) and provides lubrication during all types of eye movements.1 Goblet cells can release the ...
... weight glycoconjugates including mucins, which are secreted mainly by conjunctival goblet cells. This layer plays an important role in protecting the ocular surface from exogenous agents (bacterial or chemical) and provides lubrication during all types of eye movements.1 Goblet cells can release the ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
... antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody responses (unpublished data). Thus, it is unlikely that the data can be exp ...
... antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody responses (unpublished data). Thus, it is unlikely that the data can be exp ...
An In vitro Study on Chick Somite Ability to Express Cerberus
... these factors may be translated and exert a specific role in neural differentiation. Therefore, it seems that somites could retain their in vitro ability for expression of such factors at mRNA level. In another study, it was reported that noggin 4 was expressed during the early development of the ch ...
... these factors may be translated and exert a specific role in neural differentiation. Therefore, it seems that somites could retain their in vitro ability for expression of such factors at mRNA level. In another study, it was reported that noggin 4 was expressed during the early development of the ch ...
How to present a scientific paper
... How might ErbB2 regulate glycolysis? • Lactate dehydrogenase A (LDH-A) is a key glycolytic enzyme, and its expression is increased in mouse mammary epithelial cells that overexpress a form of ErbB2 • Heat shock factor 1 (HSF1) is a transcription factor that regulates glucose metabolism which is its ...
... How might ErbB2 regulate glycolysis? • Lactate dehydrogenase A (LDH-A) is a key glycolytic enzyme, and its expression is increased in mouse mammary epithelial cells that overexpress a form of ErbB2 • Heat shock factor 1 (HSF1) is a transcription factor that regulates glucose metabolism which is its ...
Cell-surface location of Listeria-specific protein p60
... of immunogold-labelled cells (Fig. 3 d ) . The maximum density of gold grains on the cell surface was estimated to be about 300 per pm2 for both ATCC 19111 and the rough mutant SLCC 5782. The specificity of immunogold labelling was established by control experiments in which the anti-p60 antiserum w ...
... of immunogold-labelled cells (Fig. 3 d ) . The maximum density of gold grains on the cell surface was estimated to be about 300 per pm2 for both ATCC 19111 and the rough mutant SLCC 5782. The specificity of immunogold labelling was established by control experiments in which the anti-p60 antiserum w ...
Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells
... was confined to the BM KSL HSCs and progenitor cells, and, importantly, long-term repopulating (LTR)–HSCs were exclusively present in the EndomucinCD34KSL population. Notably, in the yolk sac, Endomucin expression separated multipotential hematopoietic cells from committed erythroid progenitors in ...
... was confined to the BM KSL HSCs and progenitor cells, and, importantly, long-term repopulating (LTR)–HSCs were exclusively present in the EndomucinCD34KSL population. Notably, in the yolk sac, Endomucin expression separated multipotential hematopoietic cells from committed erythroid progenitors in ...
FUNGI
... Heterokaryotic - Hetero refers to different; and karyo refers to the nuclei. Heterokaryotic means that there are nuclei from different individuals in the same hyphae. Homothallic - Refers to species in which individuals can mate with themselves Hyphae - Thin filaments of a fungus that form its body ...
... Heterokaryotic - Hetero refers to different; and karyo refers to the nuclei. Heterokaryotic means that there are nuclei from different individuals in the same hyphae. Homothallic - Refers to species in which individuals can mate with themselves Hyphae - Thin filaments of a fungus that form its body ...
jxb.oxfordjournals.org
... Fig. S1I). qRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by ...
... Fig. S1I). qRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by ...
Early Cell Cycle Entry and Cell Division Murine Neonatal
... In the CFSE experiments described in the previous section, division peaks were not detectable among either neonatal or adult cultures until ⱖ40 h. The cell population present at this time is the cumulative product of the ongoing processes of cell survival, cell proliferation, and cell death. For exa ...
... In the CFSE experiments described in the previous section, division peaks were not detectable among either neonatal or adult cultures until ⱖ40 h. The cell population present at this time is the cumulative product of the ongoing processes of cell survival, cell proliferation, and cell death. For exa ...
Accurate Cell Division in E. Coli: How Does a Bacterium Know
... Modelling the Min System • Many mathematical models of the Min system Howard et al: Phys. Rev. Lett. (2001) ...
... Modelling the Min System • Many mathematical models of the Min system Howard et al: Phys. Rev. Lett. (2001) ...
The importance of Src homology 2 domain-containing
... 14 eukaryotic proteins.22 They were predicted to feature a high content of ␣-helices and to participate in protein-protein interactions.22,23 Since then, more than 1300 SAM-containing proteins have been identified in various organisms, ranging from yeast to humans. SAM domains can be found in all su ...
... 14 eukaryotic proteins.22 They were predicted to feature a high content of ␣-helices and to participate in protein-protein interactions.22,23 Since then, more than 1300 SAM-containing proteins have been identified in various organisms, ranging from yeast to humans. SAM domains can be found in all su ...
Blue Light-Dependent Nuclear Positioning in
... that the nucleus was located at the center of the bottom of each cell (Fig. 1D). For convenience, this position is termed the ‘dark position’. Chloroplasts were distributed along the inner peliclinal wall and the lower half of the anticlinal walls. On the other hand, after irradiation of dark-adapte ...
... that the nucleus was located at the center of the bottom of each cell (Fig. 1D). For convenience, this position is termed the ‘dark position’. Chloroplasts were distributed along the inner peliclinal wall and the lower half of the anticlinal walls. On the other hand, after irradiation of dark-adapte ...
... tips were continuous and relatively straight (Figure 1a). On the contrary, in B-deficient plants the xylem vessels were tortuous and there were discontinuities. The same was true for the main and secondary veins of the leaves (Figures 2, 3). The vascular bundles in B deficient leaf veins were ...
The VirE3 protein of Agrobacterium mimics a host cell function
... Agrobacterium tumefaciens, Agrobacterium rhizogenes, and Rhizobium etli (Winans et al, 1987; Kalogeraki et al, 2000), but it shows no homologies to any other known genes. Recent studies suggest that, during transformation, VirE3 is exported into the host yeast (Schrammeijer et al, 2003) and plant ce ...
... Agrobacterium tumefaciens, Agrobacterium rhizogenes, and Rhizobium etli (Winans et al, 1987; Kalogeraki et al, 2000), but it shows no homologies to any other known genes. Recent studies suggest that, during transformation, VirE3 is exported into the host yeast (Schrammeijer et al, 2003) and plant ce ...
AAV User Manual - Vigene Biosciences
... transducible. With some cell lines a higher MOI might be needed. Look for the highest transduction with minimal cell death. With some cell lines, high transduction levels cannot be achieved. 4. Use the minimum concentration of FBS that the cells can withstand when performing the transduction. F ...
... transducible. With some cell lines a higher MOI might be needed. Look for the highest transduction with minimal cell death. With some cell lines, high transduction levels cannot be achieved. 4. Use the minimum concentration of FBS that the cells can withstand when performing the transduction. F ...
Transendothelial Migration Surface Determinants During Activated T
... previously described (2), using a mixture of mAb with specificities against CD8 (OKT8), HLA-DR (L243), CD14 (63D3), and CD11b (OKM1), all purchased from the American Type Culture Collection (ATCC, Manassas, VA); and CD16 (B73.1), kindly provided by Dr. G. Trinchieri (Fox Chase Cancer Center, Philade ...
... previously described (2), using a mixture of mAb with specificities against CD8 (OKT8), HLA-DR (L243), CD14 (63D3), and CD11b (OKM1), all purchased from the American Type Culture Collection (ATCC, Manassas, VA); and CD16 (B73.1), kindly provided by Dr. G. Trinchieri (Fox Chase Cancer Center, Philade ...
isolation and genetic analysis of mutant strains in gametic
... to preclude their genetic characterization. Unfortunately, the unsuspected presence of non-selective lethal genes in the parental strain has limited the kinds of genetic information we can obtain from these zygotes. Nonetheless, the present report serves to establish that sexual differentiation in C ...
... to preclude their genetic characterization. Unfortunately, the unsuspected presence of non-selective lethal genes in the parental strain has limited the kinds of genetic information we can obtain from these zygotes. Nonetheless, the present report serves to establish that sexual differentiation in C ...
Factors PU.1, Spi-B, and Spi-C Transformation
... mice, Em–Spi-C transgenic stud males were bred with PUB females to generate Em–Spi-C+ Sfpi1+/2 Spib+/2 mice, which were back-crossed to PUB mice to generate Em–Spi-C+ PUB mice. Mice were generated for the experiments described in this paper by continuous pair mating of Em– Spi-C+ PUB males with PUB ...
... mice, Em–Spi-C transgenic stud males were bred with PUB females to generate Em–Spi-C+ Sfpi1+/2 Spib+/2 mice, which were back-crossed to PUB mice to generate Em–Spi-C+ PUB mice. Mice were generated for the experiments described in this paper by continuous pair mating of Em– Spi-C+ PUB males with PUB ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.