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Isolation, Characterization, and Expression of cDNAs Encoding
... through the Golgi complex (42). A number of the mammalian Golgi glycosyltransferases havebeen cloned recently (1, 7, 17, 21, 25, 32, 45, 46, 53, 56) allowing a comparison of the polypeptide structures and the ability to examine the regulation of transferase expression in relation to terminal oligosa ...
... through the Golgi complex (42). A number of the mammalian Golgi glycosyltransferases havebeen cloned recently (1, 7, 17, 21, 25, 32, 45, 46, 53, 56) allowing a comparison of the polypeptide structures and the ability to examine the regulation of transferase expression in relation to terminal oligosa ...
Studies on the Bacterial Component of Soybean Root
... The organization. of bacteroids in mature, nitrogen-$xing nodules In microtome sections of nodules examined in the light microscope, the bacteriods were apparently randomly packed into the cytoplasm in the host cells. Remnants of host nuclei were still visible and there were several small vacuoles i ...
... The organization. of bacteroids in mature, nitrogen-$xing nodules In microtome sections of nodules examined in the light microscope, the bacteriods were apparently randomly packed into the cytoplasm in the host cells. Remnants of host nuclei were still visible and there were several small vacuoles i ...
wingless acts through the shaggy/zeste-white 3
... Fig. 1. Spatial organization of the leg imaginal disc revealed by wg expression. (A) Schematic representation of the spatial relationship between cells expressing wg and the imaginal disc primordium in the embryo. The secreted Wg protein provides a positional signal to recruit nearby cells into the ...
... Fig. 1. Spatial organization of the leg imaginal disc revealed by wg expression. (A) Schematic representation of the spatial relationship between cells expressing wg and the imaginal disc primordium in the embryo. The secreted Wg protein provides a positional signal to recruit nearby cells into the ...
अध्ययन-सामग्री केन्द्रीय विद्यालय संगठन अहमदाबाद संभाग
... Q.1 Which of the following is an example of physical change? a. Mixing baking soda and vinegar together, and this causes bubbles and foam. b. A glass cup falls from the counter and shatters on the ground. c. Lighting a piece of paper on fire and the paper burns up and leaves ashes. d. Baking a birth ...
... Q.1 Which of the following is an example of physical change? a. Mixing baking soda and vinegar together, and this causes bubbles and foam. b. A glass cup falls from the counter and shatters on the ground. c. Lighting a piece of paper on fire and the paper burns up and leaves ashes. d. Baking a birth ...
Cell Biology
... How much water do you use? You could not live without a supply of freshwater. You drink water when you’re thirsty because every cell in your body needs it. You also use water every day for other things besides drinking. Do you know how much water you use each day? Find out by following the steps bel ...
... How much water do you use? You could not live without a supply of freshwater. You drink water when you’re thirsty because every cell in your body needs it. You also use water every day for other things besides drinking. Do you know how much water you use each day? Find out by following the steps bel ...
Challenges in Endometrial biopsy
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
Identification of a Mid-anaphase Checkpoint in
... length, the nucleus remained rounded in shape as it traversed the neck of the dividing cell (Fig. 2 C). The time from nuclear protrusion to the formation of this rounded sausage nucleus took 10 6 6 min (Fig. 4 A). Interestingly, the nucleus continued to maintain this round to sausageshaped morpholog ...
... length, the nucleus remained rounded in shape as it traversed the neck of the dividing cell (Fig. 2 C). The time from nuclear protrusion to the formation of this rounded sausage nucleus took 10 6 6 min (Fig. 4 A). Interestingly, the nucleus continued to maintain this round to sausageshaped morpholog ...
ARVO 2017 Annual Meeting Abstracts 306 Lens Physiology and
... Methods: Lenses from wild type mice and Cx46fs380 heterozygotes and homozygotes were studied at 1-3 months of age to determine refractive properties (by photographing the lenses above a 200-mesh electron microscopy grid), focusing ability (by laser scanning), and membrane potential, gap junctional c ...
... Methods: Lenses from wild type mice and Cx46fs380 heterozygotes and homozygotes were studied at 1-3 months of age to determine refractive properties (by photographing the lenses above a 200-mesh electron microscopy grid), focusing ability (by laser scanning), and membrane potential, gap junctional c ...
The ANGUSTIFOLIA gene of Arabidopsis, a
... Tsukaya, 1998, 2002). Using the techniques of developmental genetics to unravel the details of leaf morphogenesis (Tsukaya, 1995) we previously showed that two genes are responsible in Arabidopsis for the polarityspeci®c expansion of leaves (Tsukaya et al., 1994; Tsuge et al., 1996). They are the AN ...
... Tsukaya, 1998, 2002). Using the techniques of developmental genetics to unravel the details of leaf morphogenesis (Tsukaya, 1995) we previously showed that two genes are responsible in Arabidopsis for the polarityspeci®c expansion of leaves (Tsukaya et al., 1994; Tsuge et al., 1996). They are the AN ...
Zasp is required for the assembly of functional integrin adhesion sites
... staining typical of integrin adhesion sites, with bright foci along the cell edge and streaks in areas of potentially increased local forces (Fig. 1 A). In contrast, S2 cells spread on concanavalin A do not exhibit these integrin adhesion sites (Fig. 1 B). Instead of distinct foci and streaks, βPS i ...
... staining typical of integrin adhesion sites, with bright foci along the cell edge and streaks in areas of potentially increased local forces (Fig. 1 A). In contrast, S2 cells spread on concanavalin A do not exhibit these integrin adhesion sites (Fig. 1 B). Instead of distinct foci and streaks, βPS i ...
The lysosomal signaling anchor p18/LAMTOR1 controls epidermal
... glycogen granules in the mutant cells (Fig. 4C). These unusual accumulations of undigested materials suggest that loss of p18 suppresses degradation of cellular components via autophagy and/or the endosome system. To address this possibility, we examined the effects of loss of p18 on autophagy in pr ...
... glycogen granules in the mutant cells (Fig. 4C). These unusual accumulations of undigested materials suggest that loss of p18 suppresses degradation of cellular components via autophagy and/or the endosome system. To address this possibility, we examined the effects of loss of p18 on autophagy in pr ...
MECHANISMS OF PATTERN FORMATION IN PLANT
... 2. Feedback signaling in boundary formation: The engrailed (en) gene, which encodes a homeodomain transcription factor, is expressed in cells along the anterior margin of the parasegment. These cells also express the segment polarity gene hedgehog (hh) and secrete Hh protein. Hh activates and mainta ...
... 2. Feedback signaling in boundary formation: The engrailed (en) gene, which encodes a homeodomain transcription factor, is expressed in cells along the anterior margin of the parasegment. These cells also express the segment polarity gene hedgehog (hh) and secrete Hh protein. Hh activates and mainta ...
microcircuitry of bipolar cells in cat retina1
... the method requiresaccumulating cells at different eccentricities and from different retinas, it is unknown how regular the features of a particular type might be and whether all types are present at every point in the retina. We have taken a complementary approach, that of reconstructing a group of ...
... the method requiresaccumulating cells at different eccentricities and from different retinas, it is unknown how regular the features of a particular type might be and whether all types are present at every point in the retina. We have taken a complementary approach, that of reconstructing a group of ...
COVENANT UNIVERSITY COLLEGE OF SCIENCE AND
... The third module, explains steroids transformation, whole cell and immobilized cells, bioassays in industrial production, quality control, microbiological standards and specifications. B. ...
... The third module, explains steroids transformation, whole cell and immobilized cells, bioassays in industrial production, quality control, microbiological standards and specifications. B. ...
Direct Comparison between T Cell Control of Hepatitis B Virus +CD8
... (Cambridge Research Biochemicals, Cleveland, U.K). rIL-2 (R&D Systems, Abingdon, U.K.) was added at 30 U/ml on days 3 and 6 of culture. After 10 d of stimulation, the specificity of the lines was ascertained by using a specific 18–27 tetramer that allowed enumeration of the number of epitope-specifi ...
... (Cambridge Research Biochemicals, Cleveland, U.K). rIL-2 (R&D Systems, Abingdon, U.K.) was added at 30 U/ml on days 3 and 6 of culture. After 10 d of stimulation, the specificity of the lines was ascertained by using a specific 18–27 tetramer that allowed enumeration of the number of epitope-specifi ...
Meristem-Specific Suppression of Mitosis and a
... which separate in large numbers from the caps of species such as cereals and legumes, are not a degenerate waste product (Knudson, 1919). Instead, they represent the ultimate step in root cap development. Upon separation from the cap, these unusual cells develop into a uniquely differentiated and li ...
... which separate in large numbers from the caps of species such as cereals and legumes, are not a degenerate waste product (Knudson, 1919). Instead, they represent the ultimate step in root cap development. Upon separation from the cap, these unusual cells develop into a uniquely differentiated and li ...
Human cytomegalovirus reactivation from latency
... 1 % L-glutamine, 1 % sodium pyruvate, 50 μM βmercaptoethanol, 10 % foetal bovine serum (FBS) and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin). For infection experiments, THP-1 cells were seeded into 6-well plates to produce a final density of 1.7 × 106 cells/ml. Cell differentiation int ...
... 1 % L-glutamine, 1 % sodium pyruvate, 50 μM βmercaptoethanol, 10 % foetal bovine serum (FBS) and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin). For infection experiments, THP-1 cells were seeded into 6-well plates to produce a final density of 1.7 × 106 cells/ml. Cell differentiation int ...
... 2. Vascular tissue also provide structural support to plant. Vascular tissues forms a sort of skeleton and provides support to root, stem and leaves. It also enable plant to grow upright i.e. become taller. Q59: Is xylem (or phloem) homogenous tissue or heterogeneous tissue? Answer: Xylem and phloem ...
Skin
... – Basal cell • Most common skin cancer – sun exposure • 30% of all Caucasians in their lifetime • Cells in the stratum basale layer invade the dermis & epidermis • Shiny, dome shaped nodules that develop a central ulcer • Metastasis seldom occurs ...
... – Basal cell • Most common skin cancer – sun exposure • 30% of all Caucasians in their lifetime • Cells in the stratum basale layer invade the dermis & epidermis • Shiny, dome shaped nodules that develop a central ulcer • Metastasis seldom occurs ...
Understanding the role of cholesterol in cellular biomechanics and
... functions [92]. They have been shown to work as platforms involved with signaling in diverse cellular processes, such as immune regulation, cytoskeleton reorganization, cell cycle control, as well as membrane trafficking and fusion events, among others [27,70,95,105,109]. The association or dissocia ...
... functions [92]. They have been shown to work as platforms involved with signaling in diverse cellular processes, such as immune regulation, cytoskeleton reorganization, cell cycle control, as well as membrane trafficking and fusion events, among others [27,70,95,105,109]. The association or dissocia ...
A Cell Based Immunocytochemical Assay for Monitoring Kinase
... ABSTRACT Protein kinases play an important role in many disease processes, and are a primary target for drug development. Because cellular phosphorylation cascades are complex multidirectional pathways, the behavior of a drug in a biochemical enzyme assay may not accurately reflect its performance i ...
... ABSTRACT Protein kinases play an important role in many disease processes, and are a primary target for drug development. Because cellular phosphorylation cascades are complex multidirectional pathways, the behavior of a drug in a biochemical enzyme assay may not accurately reflect its performance i ...
The integrin–actin connection, an eternal love affair
... into the cytoplasmic domain of b1 and b7-integrins reduced membrane protrusion, cell polarization and consequently cell migration (Calderwood et al., 2001). Filamin can induce reorganization of the cytoskeleton by different pathways. First, ®lamin might stimulate changes of the cytoskeleton by provi ...
... into the cytoplasmic domain of b1 and b7-integrins reduced membrane protrusion, cell polarization and consequently cell migration (Calderwood et al., 2001). Filamin can induce reorganization of the cytoskeleton by different pathways. First, ®lamin might stimulate changes of the cytoskeleton by provi ...
salinity change and cell volume: the response of tissues from the
... perfusion chamber and the cells were allowed to settle for 15 min, during which time some of the free-floating cells attached to the coverslip. Cells that attached could be categorized into two basic morphologies: (1) larger cells (approximately 20 µm diameter) that settled on the coverslip and deve ...
... perfusion chamber and the cells were allowed to settle for 15 min, during which time some of the free-floating cells attached to the coverslip. Cells that attached could be categorized into two basic morphologies: (1) larger cells (approximately 20 µm diameter) that settled on the coverslip and deve ...
Protection of Nitrate-Reducing Fe(II)
... form of biological soil crusts (BSCs), a cohesive surface layer of soil (millimeters thick) in which mineral particles are held together by various microbial filaments, their sheaths, and extracellular polysaccharides (Belnap et al., 2003). Today, BSCs are restricted to arid and semiarid environment ...
... form of biological soil crusts (BSCs), a cohesive surface layer of soil (millimeters thick) in which mineral particles are held together by various microbial filaments, their sheaths, and extracellular polysaccharides (Belnap et al., 2003). Today, BSCs are restricted to arid and semiarid environment ...
Materials and methods - HAL
... Primary culture of cerebellar granule neurons - Media used for the culture of cerebellar granule neurons (CGN) was based on Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10 unit/ml penicillin, 10 µg/ml streptomycin, 2 mM L-glutamine, and 10 mM HEPES (K5 medium). KCl was added to a ...
... Primary culture of cerebellar granule neurons - Media used for the culture of cerebellar granule neurons (CGN) was based on Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10 unit/ml penicillin, 10 µg/ml streptomycin, 2 mM L-glutamine, and 10 mM HEPES (K5 medium). KCl was added to a ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.