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Quantitative phase microscopy – a new tool for investigating
... cellular morphology under experimental conditions. As an example, the proliferative properties of smooth muscle cells have been evaluated using QPM to track growth and confluency of cell cultures. QPM has also been used to investigate erythrocyte cell volume and morphology in different osmotic envir ...
... cellular morphology under experimental conditions. As an example, the proliferative properties of smooth muscle cells have been evaluated using QPM to track growth and confluency of cell cultures. QPM has also been used to investigate erythrocyte cell volume and morphology in different osmotic envir ...
- Mochtar Riady Institute
... note that they were still functional in regulating MDDC function similarly to the fulllength AFP derived from purified human cord blood serum, which presumably are glycosylated. These results indicated that glycosylation of AFP seems to be unnecessary in their immunoregulatory function. Our result ...
... note that they were still functional in regulating MDDC function similarly to the fulllength AFP derived from purified human cord blood serum, which presumably are glycosylated. These results indicated that glycosylation of AFP seems to be unnecessary in their immunoregulatory function. Our result ...
1. This cartoon shows Complex I in the ETC, in its two alternative
... b. The two conformations of Complex I depend on whether Q is reduced or oxidized. Indicate which is which, by writing “Reduced” or “Oxidized” next to each Q. 3pts c. Rotenone is a poison that works by blocking electron transfer from proteins with Fe-S centers. These proteins pass electrons from NADH ...
... b. The two conformations of Complex I depend on whether Q is reduced or oxidized. Indicate which is which, by writing “Reduced” or “Oxidized” next to each Q. 3pts c. Rotenone is a poison that works by blocking electron transfer from proteins with Fe-S centers. These proteins pass electrons from NADH ...
Stimulated release of fluorescently labeled IgE fragments that
... immunoglobulin E (IgE) results in slow but highly efficient accumulation of labeled IgE fragments in a pool of acidic peripheral vesicles that are visible by fluorescence microscopy after raising endosomal pH with ammonium chloride. Stimulation of cells containing these FITC-IgE fragments by aggrega ...
... immunoglobulin E (IgE) results in slow but highly efficient accumulation of labeled IgE fragments in a pool of acidic peripheral vesicles that are visible by fluorescence microscopy after raising endosomal pH with ammonium chloride. Stimulation of cells containing these FITC-IgE fragments by aggrega ...
Molecular signatures of T-cell inhibition in HIV-1 infection
... [21,27,28]. The control of viral load in HIV and SIV infections correlates with reduced PD-1 expression on virusspecific CTLs, and PD-1 blockade results in enhanced HIV- or SIV-specific CTL proliferative responses [21,27,28]. Recent findings have extended the observation that T cells primed by HIV-p ...
... [21,27,28]. The control of viral load in HIV and SIV infections correlates with reduced PD-1 expression on virusspecific CTLs, and PD-1 blockade results in enhanced HIV- or SIV-specific CTL proliferative responses [21,27,28]. Recent findings have extended the observation that T cells primed by HIV-p ...
Nonlysosomal Vesicles (Acidosomes) Are Involved
... (1) to follow the time course of the pH changes in phagosomes (2-5) and endosomes ( l, 4, 6-8) of various cells suggest that the pH of a vesicle or vacuole that derives from the cell surface often becomes acid before such an organelle fuses with lysosomes. Thus the mechanism for the initial acidific ...
... (1) to follow the time course of the pH changes in phagosomes (2-5) and endosomes ( l, 4, 6-8) of various cells suggest that the pH of a vesicle or vacuole that derives from the cell surface often becomes acid before such an organelle fuses with lysosomes. Thus the mechanism for the initial acidific ...
Nanomaterials for Cardiac Myocyte Tissue Engineering
... abundant extracardiac protein, developing scaffolds with this material should provide a physiologically relevant scenario when experimenting with CMs [38]. Furthermore, collagen types I and III make up the majority of scar tissue formed following an infarction [39]. Gelatin is a common natural mater ...
... abundant extracardiac protein, developing scaffolds with this material should provide a physiologically relevant scenario when experimenting with CMs [38]. Furthermore, collagen types I and III make up the majority of scar tissue formed following an infarction [39]. Gelatin is a common natural mater ...
The maternal muscle determinant in the ascidian egg
... (posterior-vegetal, shown in green) blastomeres, while the secondary muscle cells originate from A-line (anterior-vegetal, red) and b-line (posterior-animal, orange) blastomeres. macho-1 is involved only in fate specification of the primary muscle cells, as the mRNA is partitioned into the B-line bl ...
... (posterior-vegetal, shown in green) blastomeres, while the secondary muscle cells originate from A-line (anterior-vegetal, red) and b-line (posterior-animal, orange) blastomeres. macho-1 is involved only in fate specification of the primary muscle cells, as the mRNA is partitioned into the B-line bl ...
Emerging regulatory mechanisms in ubiquitin
... Cell Sci. 125, 249-254). ʻThe role of ubiquitylation in receptor endocytosis and endosomal sortingʼ by Kaisa Haglund and Ivan Dikic (J. Cell Sci. 125, 265-275). ʻCellular functions of the DUBsʼ by Michael J. Clague et al. (J. Cell Sci. 125, 277-286). ʻHECT and RING finger families of E3 ubiquitin li ...
... Cell Sci. 125, 249-254). ʻThe role of ubiquitylation in receptor endocytosis and endosomal sortingʼ by Kaisa Haglund and Ivan Dikic (J. Cell Sci. 125, 265-275). ʻCellular functions of the DUBsʼ by Michael J. Clague et al. (J. Cell Sci. 125, 277-286). ʻHECT and RING finger families of E3 ubiquitin li ...
Parasitic fungi on roses - Formatex Research Center
... harvested commodities, such as grapes, tomatoes, strawberries, cucumbers, bulb flowers, cut flowers and ornamental plants [24]. The broad host range of B. cinerea results in great economic losses, not only during growth but also during storage and transportation of products [25]. Necrotrophs kill th ...
... harvested commodities, such as grapes, tomatoes, strawberries, cucumbers, bulb flowers, cut flowers and ornamental plants [24]. The broad host range of B. cinerea results in great economic losses, not only during growth but also during storage and transportation of products [25]. Necrotrophs kill th ...
Apigenin
... effects of various flavonoids on antigen-specific proliferation and interferon-gamma (IFN-gamma) production by human and murine autoreactive T cells were evaluated in vitro. T-cell responses were evaluated for the human autoantigen alpha B-crystallin, a candidate autoantigen in multiple sclerosis, a ...
... effects of various flavonoids on antigen-specific proliferation and interferon-gamma (IFN-gamma) production by human and murine autoreactive T cells were evaluated in vitro. T-cell responses were evaluated for the human autoantigen alpha B-crystallin, a candidate autoantigen in multiple sclerosis, a ...
Cell Lineage and Fate Map of the Primary
... of early development known as spiral cleavage. Animals with spiral cleavage have diverse body plans, and homologous embryonic cells can be readily identified among distantly related animals. Spiralian embryos are particularly amenable to studies of fate-mapping, and larval fates of identified cells ...
... of early development known as spiral cleavage. Animals with spiral cleavage have diverse body plans, and homologous embryonic cells can be readily identified among distantly related animals. Spiralian embryos are particularly amenable to studies of fate-mapping, and larval fates of identified cells ...
Formation of Lys63-linked polyubiquitin chains on Gap1p permease
... was measured by incorporation of [14C]-citrulline (20 µM) before (t=0) and at several times after addition of (NH4)2SO4. Wt (䊏); npi2 (ⵧ), and npi2 transformed with YCpJYS-22 carrying the cloned NPI2 gene (䊉). Results are percentages of initial activities per ml culture. (B) Total protein extracts w ...
... was measured by incorporation of [14C]-citrulline (20 µM) before (t=0) and at several times after addition of (NH4)2SO4. Wt (䊏); npi2 (ⵧ), and npi2 transformed with YCpJYS-22 carrying the cloned NPI2 gene (䊉). Results are percentages of initial activities per ml culture. (B) Total protein extracts w ...
Gastric Acid Secretion: Activation and Inhibition
... increase in gene transcription [15]. Gastrin also stimulates ECL cell HDC activity in vitro [16]. Its rapid action in vitro suggests that perhaps, besides increase of gene transcription, there is also activation of pre-existing enzyme, similar to the regulation of other decarboxylases [17]. Growth o ...
... increase in gene transcription [15]. Gastrin also stimulates ECL cell HDC activity in vitro [16]. Its rapid action in vitro suggests that perhaps, besides increase of gene transcription, there is also activation of pre-existing enzyme, similar to the regulation of other decarboxylases [17]. Growth o ...
Audible Sound in Form of Music Can Influence Microbial Growth
... can be viewed as a disturbance transported through a medium via particle interaction and hence it can be characterized as a mechanical wave. Based on its frequency, sound can be broadly categorized into three categories: infrasound (up to 20 Hz), audible sound (20-20,000 Hz) and ultrasound (>20,000 ...
... can be viewed as a disturbance transported through a medium via particle interaction and hence it can be characterized as a mechanical wave. Based on its frequency, sound can be broadly categorized into three categories: infrasound (up to 20 Hz), audible sound (20-20,000 Hz) and ultrasound (>20,000 ...
9 NCD Ch 6 | The cortical plate
... In our Golgi material, translocating cells were rarely seen in the intermediate zone, but cortical plate neu rons, predominantly pyramidal cells, were impreg nated in large numbers. The majority of pyramidal tells on day E17 (Fig. 6-1A-C) had a single, short apical dendrite growing into the outer ...
... In our Golgi material, translocating cells were rarely seen in the intermediate zone, but cortical plate neu rons, predominantly pyramidal cells, were impreg nated in large numbers. The majority of pyramidal tells on day E17 (Fig. 6-1A-C) had a single, short apical dendrite growing into the outer ...
SYSTEM ANALYSIS MORPHOGENESIS BREAST CANCER
... cells Excludes qualitative uniqueness of these states at the "cell." Second, in systems such as EPE properties of any cell are determined by all the other cells. Hence, for any of many differentiated cells need some preliminary development of type EPE increase integrity and which was evident in the ...
... cells Excludes qualitative uniqueness of these states at the "cell." Second, in systems such as EPE properties of any cell are determined by all the other cells. Hence, for any of many differentiated cells need some preliminary development of type EPE increase integrity and which was evident in the ...
The allocation of early blastomeres to the ectoderm and endoderm
... labeled cells within the gut. SMCs such as the blastocoelar and pigment cells disperse throughout the embryo, but because veg2 descendants always form some foregut tissues, a position of origin could be assigned to these mesenchymal cells. Where multiple veg1 cells were labeled within a single tier, ...
... labeled cells within the gut. SMCs such as the blastocoelar and pigment cells disperse throughout the embryo, but because veg2 descendants always form some foregut tissues, a position of origin could be assigned to these mesenchymal cells. Where multiple veg1 cells were labeled within a single tier, ...
The Pore Size of Non-Graminaceous Plant Cell
... and Skoog medium (Murashige and Skoog, 1962) containing KH2PO4 (0.4 g L21) and Suc (40 g L21). Cells were maintained at a high specific mean growth rate by subculturing every 2 d into fresh medium (1 volume of cells into 2.5 volumes of medium) containing boric acid (100 mm, control cells) or no adde ...
... and Skoog medium (Murashige and Skoog, 1962) containing KH2PO4 (0.4 g L21) and Suc (40 g L21). Cells were maintained at a high specific mean growth rate by subculturing every 2 d into fresh medium (1 volume of cells into 2.5 volumes of medium) containing boric acid (100 mm, control cells) or no adde ...
Introduction
... The tight junctions regulate the passage of ions between the cells. They do not always form an absolute diffusion barrier, but are semipermeable and allow selective passage of certain solutes, but not others (Balda and Matter, 1998). Exchange of larger molecules from one domain to the other is perfo ...
... The tight junctions regulate the passage of ions between the cells. They do not always form an absolute diffusion barrier, but are semipermeable and allow selective passage of certain solutes, but not others (Balda and Matter, 1998). Exchange of larger molecules from one domain to the other is perfo ...
MagneHis™ Protein Purification System Technical Manual
... Samples can also be processed using a robotic platform for high-throughput applications. The MagneHis™ System is useful for screening multiple clones for expression, optimizing expression conditions (temperature, media, host strain, etc.) and the initial screening of mutant clones. Bacterial, insect ...
... Samples can also be processed using a robotic platform for high-throughput applications. The MagneHis™ System is useful for screening multiple clones for expression, optimizing expression conditions (temperature, media, host strain, etc.) and the initial screening of mutant clones. Bacterial, insect ...
JMS 68/1 pp. 087-094 FINAL - Oxford Academic
... The opening of the pericardium into the renopericardial duct is about 5 µm wide. The renopericardial duct is composed of very flat cells with an irregularly shaped nucleus occupying most of the cytoplasm. Apically, there are belt desmosomes and septate junctions between adjacent cells. Whereas the c ...
... The opening of the pericardium into the renopericardial duct is about 5 µm wide. The renopericardial duct is composed of very flat cells with an irregularly shaped nucleus occupying most of the cytoplasm. Apically, there are belt desmosomes and septate junctions between adjacent cells. Whereas the c ...
Isolation, Characterization, and Expression of cDNAs Encoding
... through the Golgi complex (42). A number of the mammalian Golgi glycosyltransferases havebeen cloned recently (1, 7, 17, 21, 25, 32, 45, 46, 53, 56) allowing a comparison of the polypeptide structures and the ability to examine the regulation of transferase expression in relation to terminal oligosa ...
... through the Golgi complex (42). A number of the mammalian Golgi glycosyltransferases havebeen cloned recently (1, 7, 17, 21, 25, 32, 45, 46, 53, 56) allowing a comparison of the polypeptide structures and the ability to examine the regulation of transferase expression in relation to terminal oligosa ...
the Cytoskeleton in Plant Development1
... these MTOCs is unknown, but it seems that they contain microtubute-associated proteins (MAPs) that lower the critical concentration of tubutin necessary for microtubute assembly. Plants do possess y-tubulin as well but it is not confined to the MTOC, but associated with all microtubute arrays (Liu e ...
... these MTOCs is unknown, but it seems that they contain microtubute-associated proteins (MAPs) that lower the critical concentration of tubutin necessary for microtubute assembly. Plants do possess y-tubulin as well but it is not confined to the MTOC, but associated with all microtubute arrays (Liu e ...
Mice lacking the homeodomain transcription factor
... unusual string-like morphology, whereas the exocrine cells appear unaffected (data not shown). To study the effect of Nkx2.2 deficiency on the endocrine pancreas, we examined hormone expression from the four endocrine cell types using immunohistochemistry. Throughout all stages of embryonic developm ...
... unusual string-like morphology, whereas the exocrine cells appear unaffected (data not shown). To study the effect of Nkx2.2 deficiency on the endocrine pancreas, we examined hormone expression from the four endocrine cell types using immunohistochemistry. Throughout all stages of embryonic developm ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.