Bone marrow side population cells are enriched for progenitors
... proliferate in response to an acute injury such as CTX (Polesskaya et al., 2003), while the large populations of GFP+/CD45+ cells are due to the influx of donor-derived immune/inflammatory cells that migrate to the muscle after chemical damage. As expected, these populations decreased substantially ...
... proliferate in response to an acute injury such as CTX (Polesskaya et al., 2003), while the large populations of GFP+/CD45+ cells are due to the influx of donor-derived immune/inflammatory cells that migrate to the muscle after chemical damage. As expected, these populations decreased substantially ...
Nonlysosomal Vesicles (Acidosomes) Are Involved
... vacuole still attached to the oral region (or) of Paramecium caudatum. This cell had been pulsed 15 s with polystyrene latex beads (0.8/am diam) and horseradish peroxidase whose reaction product following incubation in 3,3'-diaminobenzidine and H202 formed the electron-opaque deposit lining the lumi ...
... vacuole still attached to the oral region (or) of Paramecium caudatum. This cell had been pulsed 15 s with polystyrene latex beads (0.8/am diam) and horseradish peroxidase whose reaction product following incubation in 3,3'-diaminobenzidine and H202 formed the electron-opaque deposit lining the lumi ...
Cardiac optogenetics - Biomedical Engineering
... (K⫹) channel conformations are possible with direct fluorescence labeling and total internal reflection fluorescence microscopy imaging to infer membrane voltage changes (87, 113, 116, 117). Certain applications of genetics for development of new imaging probes, i.e., genetically encoded calcium and ...
... (K⫹) channel conformations are possible with direct fluorescence labeling and total internal reflection fluorescence microscopy imaging to infer membrane voltage changes (87, 113, 116, 117). Certain applications of genetics for development of new imaging probes, i.e., genetically encoded calcium and ...
Rewiring Host Lipid Metabolism by Large Viruses
... at four time points (4, 24, 32, and 48 hpi) (see Supplemental Data Sets 3 and 4 for details). Nearly half of these (86 signals) were identified as specific metabolites by library comparison and/or coinjection with authentic standards. Forty-seven signals were tentatively assigned to a metabolite class ...
... at four time points (4, 24, 32, and 48 hpi) (see Supplemental Data Sets 3 and 4 for details). Nearly half of these (86 signals) were identified as specific metabolites by library comparison and/or coinjection with authentic standards. Forty-seven signals were tentatively assigned to a metabolite class ...
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... they were still asymmetrically distributed, with more in the anterior region of each ependymal cell, in agreement with a recent report on translational polarity (Fig. 1D) (Mirzadeh et al., 2010). We next compared the centroid position of the area containing basal bodies with that of the whole cell, ...
... they were still asymmetrically distributed, with more in the anterior region of each ependymal cell, in agreement with a recent report on translational polarity (Fig. 1D) (Mirzadeh et al., 2010). We next compared the centroid position of the area containing basal bodies with that of the whole cell, ...
Non-coding RNAs - Biology Department | UNC Chapel Hill
... Figure 2 | Biogenesis of Sm-class small nuclear RNPs. The life-cycle of Sm-class small nuclear ribonucleoproteins (snRNPs) includes both nuclear and cytoplasmic phases. Following transcription by a specialized form of RNA polymerase II, pre-snRNA transcripts are exported to the cytoplasm, with a pot ...
... Figure 2 | Biogenesis of Sm-class small nuclear RNPs. The life-cycle of Sm-class small nuclear ribonucleoproteins (snRNPs) includes both nuclear and cytoplasmic phases. Following transcription by a specialized form of RNA polymerase II, pre-snRNA transcripts are exported to the cytoplasm, with a pot ...
Cellular uptake of long-chain fatty acids: role of membrane
... The spontaneously hypertensive rat (SHR) is insulin resistant and a good model of these human syndromes. Quantitative trait loci for SHR defects in glucose and fatty acid metabolism, hypertriglyceridaemia and hypertension map to a single locus on rat chromosome 4. cDNA microarrays, congenic mapping ...
... The spontaneously hypertensive rat (SHR) is insulin resistant and a good model of these human syndromes. Quantitative trait loci for SHR defects in glucose and fatty acid metabolism, hypertriglyceridaemia and hypertension map to a single locus on rat chromosome 4. cDNA microarrays, congenic mapping ...
Lessons on longevity from budding yeast
... is blocked by deletion of SIR2. A complicating factor in the study by Lin et al.27 is that the strain background used does not show increased RLS in response to overexpression of Sir2 (ref. 26). In a strain background that responds to both dietary restriction and overexpression of Sir2, combining th ...
... is blocked by deletion of SIR2. A complicating factor in the study by Lin et al.27 is that the strain background used does not show increased RLS in response to overexpression of Sir2 (ref. 26). In a strain background that responds to both dietary restriction and overexpression of Sir2, combining th ...
Systems-Level Comparison of Host-Responses Elicited by Avian
... ligand 10 (CXCL10), IFN-a1 and -b1, and suppressor of cytokine signaling 1 and 3 (SOCS1, SOCS3), were verified by the use of real-time PCR, and the data are shown in Figure 2. To compare the cellular response to H1N1 infection versus H5N1 infection, Gene Ontology and pathway over-representation anal ...
... ligand 10 (CXCL10), IFN-a1 and -b1, and suppressor of cytokine signaling 1 and 3 (SOCS1, SOCS3), were verified by the use of real-time PCR, and the data are shown in Figure 2. To compare the cellular response to H1N1 infection versus H5N1 infection, Gene Ontology and pathway over-representation anal ...
Convergence and Extension Movements During Vertebrate
... 2. The Regional and Temporal Pattern of C&E Movements in the Mesoderm During the first 3 hours of development, the zebrafish embryo undergoes rapid synchronous cell divisions to form a mound of blastomeres atop a large syncytial yolk cell (Kimmel et al., 1995). Maternally contributed transcripts and ...
... 2. The Regional and Temporal Pattern of C&E Movements in the Mesoderm During the first 3 hours of development, the zebrafish embryo undergoes rapid synchronous cell divisions to form a mound of blastomeres atop a large syncytial yolk cell (Kimmel et al., 1995). Maternally contributed transcripts and ...
Aluminum-Induced 133-ß-D-Glucan Inhibits Cell
... transient level of callose within controls, which was also localized preferentially at PD regions (Fig. 3A). A sharp decrease to callose levels in control plants (Fig. 4B) fixed after DDG pre-treatment compared with absolute controls supports this notion. In general, in comparison with the immunogol ...
... transient level of callose within controls, which was also localized preferentially at PD regions (Fig. 3A). A sharp decrease to callose levels in control plants (Fig. 4B) fixed after DDG pre-treatment compared with absolute controls supports this notion. In general, in comparison with the immunogol ...
STUDY OF THE CELLS PROLIFERATING IN PARENT VERSUS F1
... synthesis in MLC (Table II) . In some experiments, a very weak increase in SI was still found, but since this could also be observed in control cultures containing syngeneic parental cells instead of F, cells (Table II and legend), it appears to be related to the cell treatment and not to the MLC. T ...
... synthesis in MLC (Table II) . In some experiments, a very weak increase in SI was still found, but since this could also be observed in control cultures containing syngeneic parental cells instead of F, cells (Table II and legend), it appears to be related to the cell treatment and not to the MLC. T ...
Selective kinase inhibitors as tools for neuroscience research
... the in vitro specificity of a compound against a significant proportion of the kinome either through academic facilities or via commercial inhibitor screening companies. In addition screening data has been published for many commonly used kinases inhibitors (Anastassiadis et al., 2011; Bain et al., 20 ...
... the in vitro specificity of a compound against a significant proportion of the kinome either through academic facilities or via commercial inhibitor screening companies. In addition screening data has been published for many commonly used kinases inhibitors (Anastassiadis et al., 2011; Bain et al., 20 ...
Cell Membrane Electroporation—Part 2
... and chemical extraction (such as alkaline lysis) are the principal methods. The main disadvantage of both is that cellular organelles (which are special subunits within a cell with specific functions, such as cell breathing, protein production, DNA maintenance) are also destroyed, and residual cell ...
... and chemical extraction (such as alkaline lysis) are the principal methods. The main disadvantage of both is that cellular organelles (which are special subunits within a cell with specific functions, such as cell breathing, protein production, DNA maintenance) are also destroyed, and residual cell ...
Submembraneous microtubule cytoskeleton: biochemical and
... become ‘cold stable’ as these microtubules do not depolymerise further at low temperature [28]. The exact mechanism how Ca2+ modulates these physicochemical properties in vitro are not clear. In this regard, it is important to mention that tubulin has been shown to bind two Ca2+ ions to its C-termin ...
... become ‘cold stable’ as these microtubules do not depolymerise further at low temperature [28]. The exact mechanism how Ca2+ modulates these physicochemical properties in vitro are not clear. In this regard, it is important to mention that tubulin has been shown to bind two Ca2+ ions to its C-termin ...
Cytokinesis of neuroepithelial cells can divide their basal process
... Figure 1 Basal processes of NE cells in M phase can be visualized by MPM-2 immunostaining or expression of membrane-targeted GFP. (A) Overview of the ventricular zone. A frozen section of E10.5 mouse telencephalic neuroepithelium was immunostained with MPM-2 antibody. Eight 1-mm optical sections wer ...
... Figure 1 Basal processes of NE cells in M phase can be visualized by MPM-2 immunostaining or expression of membrane-targeted GFP. (A) Overview of the ventricular zone. A frozen section of E10.5 mouse telencephalic neuroepithelium was immunostained with MPM-2 antibody. Eight 1-mm optical sections wer ...
Pap and HPV Testing
... cells and that the cells look very different from normal cells. When examined under a microscope, the cells from HSILs are often found to have more extensive changes that may be classified as moderate or severe dysplasia or as CIN-2, CIN-2/3, or CIN-3 (in order of increasing severity). Microscopic ...
... cells and that the cells look very different from normal cells. When examined under a microscope, the cells from HSILs are often found to have more extensive changes that may be classified as moderate or severe dysplasia or as CIN-2, CIN-2/3, or CIN-3 (in order of increasing severity). Microscopic ...
Embryonic stem cells in non-human primates An overview of neural
... (Evans and Kaufman, 1981). These properties render them an ideal resource for the in vitro study of developmental processes, and for transplantation therapy in numerous pathologies (Lerou and Daley, 2005). The successful derivation of ES cells was not straightforward (Hogan and Tilly, 1977). A promi ...
... (Evans and Kaufman, 1981). These properties render them an ideal resource for the in vitro study of developmental processes, and for transplantation therapy in numerous pathologies (Lerou and Daley, 2005). The successful derivation of ES cells was not straightforward (Hogan and Tilly, 1977). A promi ...
The roles of microtubules in tropisms
... examples). In addition, there are several mutations that alter cortical microtubule organization and plants with these mutations have defects in the control of directional cell elongation. For example, microtubules become very short and cells expand radially when the temperature-sensitive mor1-1 mut ...
... examples). In addition, there are several mutations that alter cortical microtubule organization and plants with these mutations have defects in the control of directional cell elongation. For example, microtubules become very short and cells expand radially when the temperature-sensitive mor1-1 mut ...
Derivation of naïve human embryonic stem cells
... resources, Agilent expression data were compared with archived array data. A set of 628 naïve Elf1 specific enriched (fourfold or greater) genes and of 639 primed Elf1 specific enriched genes were used as a yardstick against which to compare our data with that in the literature. Naïve Elf1 expressio ...
... resources, Agilent expression data were compared with archived array data. A set of 628 naïve Elf1 specific enriched (fourfold or greater) genes and of 639 primed Elf1 specific enriched genes were used as a yardstick against which to compare our data with that in the literature. Naïve Elf1 expressio ...
Cellular damage induced by cadmium and
... treatments were long enough to attain substantial metabolic changes. In such experiments, the effects caused by relatively long exposure to metal might reflect a general failure of the plant metabolism. Little is known about the early stages. Even though toxic metals can clearly induce oxidative str ...
... treatments were long enough to attain substantial metabolic changes. In such experiments, the effects caused by relatively long exposure to metal might reflect a general failure of the plant metabolism. Little is known about the early stages. Even though toxic metals can clearly induce oxidative str ...
UNIT 1 – FORCE AND MOTION (SEPUP Force and
... B-4.1 Compare DNA and RNA in terms of structure, nucleotides, and base pairs. B-4.2 Summarize the relationship among DNA, genes, and chromosomes. B-4.3 Explain how DNA functions as the code of life and the blueprint for proteins B-4.4 Summarize the basic processes involved in protein synthesis (incl ...
... B-4.1 Compare DNA and RNA in terms of structure, nucleotides, and base pairs. B-4.2 Summarize the relationship among DNA, genes, and chromosomes. B-4.3 Explain how DNA functions as the code of life and the blueprint for proteins B-4.4 Summarize the basic processes involved in protein synthesis (incl ...
Interesting Facts - Raleigh Charter High School
... It has a mutalistic symbiotic relationship with green algae called Zoochlorella. The algae live inside the paramecium in its cytoplasm and provides the Paramecium with food, while the Paramecium provides algae with movement. ...
... It has a mutalistic symbiotic relationship with green algae called Zoochlorella. The algae live inside the paramecium in its cytoplasm and provides the Paramecium with food, while the Paramecium provides algae with movement. ...